Sahar
@saharsci.bsky.social
graduate student @torontometu 🇨🇦
cell biology, EGFR regulation, single particle tracking
cell biology, EGFR regulation, single particle tracking
Reposted by Sahar
Super collaboration with @raffaeleieva.bsky.social and @fronzeslab.bsky.social on the LptDEMY complex.
rdcu.be/eRxNf here our new study on the characterization of the lipopolysaccharide holotranslocon, LptDE and the newly identified subunits LptM and LptY. Great collaboration with @fronzeslab.bsky.social @pstansfeld.bsky.social @JulienMarcoux @YvesQuentin
Structural basis of lipopolysaccharide assembly by the outer membrane translocon holo-complex
Nature Communications - The asymmetric distribution of lipopolysaccharide (LPS) on the surface of the bacterial outer membrane is essential and crucial for antibiotic resistance. Here, authors...
rdcu.be
November 24, 2025 at 4:56 PM
Super collaboration with @raffaeleieva.bsky.social and @fronzeslab.bsky.social on the LptDEMY complex.
Reposted by Sahar
Some cells are just in it for the drama!
The bottom 5 microns of cells videoed through a microscope by @EmmaKoory. The middle cell rounds up (for fun?) and subsequently rounds up to divide. We missed so much of the action by just sampling the bottom. @CellBiology
The bottom 5 microns of cells videoed through a microscope by @EmmaKoory. The middle cell rounds up (for fun?) and subsequently rounds up to divide. We missed so much of the action by just sampling the bottom. @CellBiology
November 24, 2025 at 9:19 PM
Some cells are just in it for the drama!
The bottom 5 microns of cells videoed through a microscope by @EmmaKoory. The middle cell rounds up (for fun?) and subsequently rounds up to divide. We missed so much of the action by just sampling the bottom. @CellBiology
The bottom 5 microns of cells videoed through a microscope by @EmmaKoory. The middle cell rounds up (for fun?) and subsequently rounds up to divide. We missed so much of the action by just sampling the bottom. @CellBiology
Reposted by Sahar
u-Segment3D
Universal consensus 3D segmentation of cells from 2D segmented stacks
Work on complex networks, organelles & even endothelial filopodia🤯
A solid step toward single-cell 3D spatial omics 👹
@natmethods.nature.com 2025
www.nature.com/articles/s41...
Universal consensus 3D segmentation of cells from 2D segmented stacks
Work on complex networks, organelles & even endothelial filopodia🤯
A solid step toward single-cell 3D spatial omics 👹
@natmethods.nature.com 2025
www.nature.com/articles/s41...
November 24, 2025 at 12:44 PM
u-Segment3D
Universal consensus 3D segmentation of cells from 2D segmented stacks
Work on complex networks, organelles & even endothelial filopodia🤯
A solid step toward single-cell 3D spatial omics 👹
@natmethods.nature.com 2025
www.nature.com/articles/s41...
Universal consensus 3D segmentation of cells from 2D segmented stacks
Work on complex networks, organelles & even endothelial filopodia🤯
A solid step toward single-cell 3D spatial omics 👹
@natmethods.nature.com 2025
www.nature.com/articles/s41...
Reposted by Sahar
📢Check out our latest #preprint list on FocalPlane
This list focusses on the latest research on bioimage analysis and data management. Let us know if there are any preprints that we should add to our list and send us recommendation for the next one!
focalplane.biologists.com/2025/11/14/m...
This list focusses on the latest research on bioimage analysis and data management. Let us know if there are any preprints that we should add to our list and send us recommendation for the next one!
focalplane.biologists.com/2025/11/14/m...
Microscopy preprints – bioimage analysis - FocalPlane
Microscopy preprints – bioimage analysis - News
focalplane.biologists.com
November 18, 2025 at 10:07 AM
📢Check out our latest #preprint list on FocalPlane
This list focusses on the latest research on bioimage analysis and data management. Let us know if there are any preprints that we should add to our list and send us recommendation for the next one!
focalplane.biologists.com/2025/11/14/m...
This list focusses on the latest research on bioimage analysis and data management. Let us know if there are any preprints that we should add to our list and send us recommendation for the next one!
focalplane.biologists.com/2025/11/14/m...
Reposted by Sahar
Free ASCB session Dec 2, 12 p.m. ET: Exploring Cellular Dynamics with Advanced Microscopy with Suliana Manley, Ben Loos & Lindy O’Claire.
🎓 Live attendees get a Certificate.
Register: www.ascb.org/ascb-meeting...
🎓 Live attendees get a Certificate.
Register: www.ascb.org/ascb-meeting...
November 19, 2025 at 8:01 PM
Free ASCB session Dec 2, 12 p.m. ET: Exploring Cellular Dynamics with Advanced Microscopy with Suliana Manley, Ben Loos & Lindy O’Claire.
🎓 Live attendees get a Certificate.
Register: www.ascb.org/ascb-meeting...
🎓 Live attendees get a Certificate.
Register: www.ascb.org/ascb-meeting...
Reposted by Sahar
Traffic deaths are policy choices.
“Helsinki hasn’t registered a single traffic-related fatality in the past year…Citing data that shows the risk of pedestrian fatality is cut in half by reducing a car’s speed from 40 to 30km/hr, city officials imposed the lower limit in most of Helsinki’s residential areas & city center in 2021.”
Helsinki just went a full year without a single traffic death
The capital city is Finnish’ed with car-related fatalities.
www.politico.eu
November 17, 2025 at 5:59 PM
Traffic deaths are policy choices.
Reposted by Sahar
This week is particularly remarkable in terms of #CellDeath and infection biology-related discoveries: biomed.news/bims-cediti/...
Highlight 1: SIGLEC12 mediates plasma membrane rupture during necroptotic cell death:
Highlight 1: SIGLEC12 mediates plasma membrane rupture during necroptotic cell death:
SIGLEC12 mediates plasma membrane rupture during necroptotic cell death - Nature
CRISPR–Cas9-based genome-wide screening shows that SIGLEC12 mediates plasma membrane rupture in cells undergoing necroptosis, possibly through a human-specific mechanism, indicating its potential as a...
www.nature.com
November 16, 2025 at 9:09 AM
This week is particularly remarkable in terms of #CellDeath and infection biology-related discoveries: biomed.news/bims-cediti/...
Highlight 1: SIGLEC12 mediates plasma membrane rupture during necroptotic cell death:
Highlight 1: SIGLEC12 mediates plasma membrane rupture during necroptotic cell death:
Reposted by Sahar
Flashback #FluorescenceFriday to some of the first light-sheet imaging we were doing. Video shows the collecting duct system of the developing kidney labeled with a pan-cytokeratin antibody. Courtesy of former talented technician Deanna Hardesty.
November 7, 2025 at 4:43 PM
Flashback #FluorescenceFriday to some of the first light-sheet imaging we were doing. Video shows the collecting duct system of the developing kidney labeled with a pan-cytokeratin antibody. Courtesy of former talented technician Deanna Hardesty.
Reposted by Sahar
Color-coded CODA maps of fallopian tubes from premenopausal (left) and postmenopausal women.
Magenta: secretory epithelial cells,
Green: ciliated epithelial cells.
More here: www.biorxiv.org/content/10.1...
Magenta: secretory epithelial cells,
Green: ciliated epithelial cells.
More here: www.biorxiv.org/content/10.1...
November 16, 2025 at 3:46 PM
Color-coded CODA maps of fallopian tubes from premenopausal (left) and postmenopausal women.
Magenta: secretory epithelial cells,
Green: ciliated epithelial cells.
More here: www.biorxiv.org/content/10.1...
Magenta: secretory epithelial cells,
Green: ciliated epithelial cells.
More here: www.biorxiv.org/content/10.1...
Reposted by Sahar
I guess it never gets old...bc we love #CellMigration
ATP has many roles: energy currency, hydrotrope, and *danger signal*
Immune cells are faster after ATP exposure, F-actin (cyan) goes to the rear for nitro boost!
www.science.org/doi/full/10....
@focalplane.bsky.social #fluorescencefriday 🧪🔬
ATP has many roles: energy currency, hydrotrope, and *danger signal*
Immune cells are faster after ATP exposure, F-actin (cyan) goes to the rear for nitro boost!
www.science.org/doi/full/10....
@focalplane.bsky.social #fluorescencefriday 🧪🔬
November 14, 2025 at 8:38 PM
I guess it never gets old...bc we love #CellMigration
ATP has many roles: energy currency, hydrotrope, and *danger signal*
Immune cells are faster after ATP exposure, F-actin (cyan) goes to the rear for nitro boost!
www.science.org/doi/full/10....
@focalplane.bsky.social #fluorescencefriday 🧪🔬
ATP has many roles: energy currency, hydrotrope, and *danger signal*
Immune cells are faster after ATP exposure, F-actin (cyan) goes to the rear for nitro boost!
www.science.org/doi/full/10....
@focalplane.bsky.social #fluorescencefriday 🧪🔬
Reposted by Sahar
Multimodal learning enables chat-based exploration of single-cell data www.nature.com/articles/s41... 🧬🖥️🧪 github.com/epigen/cellw...
November 14, 2025 at 7:01 PM
Multimodal learning enables chat-based exploration of single-cell data www.nature.com/articles/s41... 🧬🖥️🧪 github.com/epigen/cellw...
Reposted by Sahar
Independent validation of the SnxA biosensor as a sensitive reporter of PI(3,5)P₂ dynamics by Gerald R Hammond and team: www.biorxiv.org/content/10.1...
November 12, 2025 at 6:57 PM
Independent validation of the SnxA biosensor as a sensitive reporter of PI(3,5)P₂ dynamics by Gerald R Hammond and team: www.biorxiv.org/content/10.1...
Reposted by Sahar
I wrote this insight for eLife. A friend said, "Why would you want to step on that rake?". Well, I guess that's just who I am. Enjoy. elifesciences.org/articles/109...
Transgenerational Epigenetic Inheritance: Twists and turns in the story of learned avoidance
Evidence that learned avoidance of a pathogenic bacterium can be transmitted to future generations in C. elegans is growing.
elifesciences.org
November 11, 2025 at 7:37 PM
I wrote this insight for eLife. A friend said, "Why would you want to step on that rake?". Well, I guess that's just who I am. Enjoy. elifesciences.org/articles/109...
Reposted by Sahar
A new #ScienceImmunology study in mice provides insight into why malaria vaccines that contain the whole Plasmodium parasite may have limited efficacy in humans who live in endemic regions. https://scim.ag/4nONXn9
B cells targeting parasites capture spatially linked antigens to secure T cell help
Antigen location orchestrates T cell–B cell collaboration in response to large pathogens.
scim.ag
November 11, 2025 at 4:00 PM
A new #ScienceImmunology study in mice provides insight into why malaria vaccines that contain the whole Plasmodium parasite may have limited efficacy in humans who live in endemic regions. https://scim.ag/4nONXn9
Reposted by Sahar
See study by @kirstin-rollins.bsky.social, @sareenfiaz.bsky.social, Ishwaree Datta and @mmorrissey.bsky.social: rupress.org/jcb/article/...
Target cell adhesion limits macrophage phagocytosis and promotes trogocytosis
Macrophages are presumed to attack antibody-opsonized cancer cells by phagocytosis. Rollins et al. found that macrophages attack adherent cells using trogo
rupress.org
November 10, 2025 at 3:04 PM
See study by @kirstin-rollins.bsky.social, @sareenfiaz.bsky.social, Ishwaree Datta and @mmorrissey.bsky.social: rupress.org/jcb/article/...
www.sciencedirect.com/science/arti...
sometimes a review is just so good you have to share it
sometimes a review is just so good you have to share it
EGFR Activation and Conformational “Convergence”: An Emerging Allosteric Model From Cancer Mutations
Since its isolation nearly 50 years ago, the Epidermal Growth Factor Receptor (EGFR) has become one of the most extensively studied oncogenes. However…
www.sciencedirect.com
November 7, 2025 at 3:31 AM
www.sciencedirect.com/science/arti...
sometimes a review is just so good you have to share it
sometimes a review is just so good you have to share it
performance anxiety is so silly cuz why is it the one time im observed i mess up in ways ive never had before doing the thing a million times 😬😂
November 6, 2025 at 7:57 PM
performance anxiety is so silly cuz why is it the one time im observed i mess up in ways ive never had before doing the thing a million times 😬😂
Reposted by Sahar
I love finding a paper published years ago (5, 10, 15, 20) that isn't famous or field changing or has a ton of citations but when you read it is just awesome. Like, beautifully designed & executed & written. Best part is that there are so many papers like that lurking in the literature.
November 6, 2025 at 6:13 PM
I love finding a paper published years ago (5, 10, 15, 20) that isn't famous or field changing or has a ton of citations but when you read it is just awesome. Like, beautifully designed & executed & written. Best part is that there are so many papers like that lurking in the literature.
mind blown
Peiyao Fan, Yang Chen et al. @pku1898.bsky.social show that tubular ER extends into retraction fibers and #migrasomes through #microtubule-dependent ER extension and ER-plasma membrane contact sites in migrating cells. rupress.org/jcb/article/...
#ER_literature #Migration #Organelles
#ER_literature #Migration #Organelles
November 6, 2025 at 5:09 PM
mind blown
Reposted by Sahar
Janelia is hosting a course on DL for bioimage analysis www.janelia.org/you-janelia/...
Deep Learning for Microscopy Image Analysis
Topics The following will be covered extensively during lectures, exercises, and project work: Image denoising and restoration (fully supervised and self-supervised) Image translation (e.g.,
www.janelia.org
November 6, 2025 at 5:02 PM
Janelia is hosting a course on DL for bioimage analysis www.janelia.org/you-janelia/...
Reposted by Sahar
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!
www.fpbase.org/spectra/
www.fpbase.org/spectra/
FPbase Fluorescence Spectra Viewer
An interactive fluorescence spectra viewer to evaluate the spectral properties of fluorescent proteins, organic dyes, filters, and detectors.
www.fpbase.org
November 4, 2025 at 3:03 PM
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!
www.fpbase.org/spectra/
www.fpbase.org/spectra/
Reposted by Sahar
My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
November 4, 2025 at 10:40 PM
My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
Reposted by Sahar
Proud of our latest #glycotime review. Co-authored by my amazing cancer collaborator Dr. Eva Hernando and our students @faezehjame.bsky.social , Tigist Batu and Kylie Prutisto-Chang. 🧪
Glycosylation in Cancer: From Functional Roles to Therapeutic Implications - www.annualreviews.org/content/jour...
Glycosylation in Cancer: From Functional Roles to Therapeutic Implications - www.annualreviews.org/content/jour...
Glycosylation in Cancer: From Functional Roles to Therapeutic Implications
The field of cancer glycobiology aims to understand how aberrant glycosylation contributes to the development of cancer. While the significance of glycosylation to normal and pathological cellular fun...
www.annualreviews.org
November 5, 2025 at 1:03 AM
Proud of our latest #glycotime review. Co-authored by my amazing cancer collaborator Dr. Eva Hernando and our students @faezehjame.bsky.social , Tigist Batu and Kylie Prutisto-Chang. 🧪
Glycosylation in Cancer: From Functional Roles to Therapeutic Implications - www.annualreviews.org/content/jour...
Glycosylation in Cancer: From Functional Roles to Therapeutic Implications - www.annualreviews.org/content/jour...
Reposted by Sahar
Dr Donna Senger gave the next talk: Harnessing the power of macrophages for cancer therapy #CCRC2025
- 40% of a glioblastoma tumour can be recruited pro-tumour macrophages!
- inhibiting the expression of nuclear IL-33 reduces this recruitment/transformation
www.nature.com/articles/s41...
- 40% of a glioblastoma tumour can be recruited pro-tumour macrophages!
- inhibiting the expression of nuclear IL-33 reduces this recruitment/transformation
www.nature.com/articles/s41...
Glioma-derived IL-33 orchestrates an inflammatory brain tumor microenvironment that accelerates glioma progression - Nature Communications
Elevated levels of interleukin-33 have been associated with poor prognosis in patients with glioma. Here the authors show that glioma-derived IL-33 modulates a pro-tumorigenic immune microenvironment ...
www.nature.com
November 2, 2025 at 6:50 PM
Dr Donna Senger gave the next talk: Harnessing the power of macrophages for cancer therapy #CCRC2025
- 40% of a glioblastoma tumour can be recruited pro-tumour macrophages!
- inhibiting the expression of nuclear IL-33 reduces this recruitment/transformation
www.nature.com/articles/s41...
- 40% of a glioblastoma tumour can be recruited pro-tumour macrophages!
- inhibiting the expression of nuclear IL-33 reduces this recruitment/transformation
www.nature.com/articles/s41...
cant imagine the sadness at union station tonight 🫠
November 2, 2025 at 4:29 AM
cant imagine the sadness at union station tonight 🫠