Conor Sugden
@conorjsugden.bsky.social
Postdoc Research Associate in the Lennon Lab, Manchester Cell-Matrix Centre, University of Manchester
Lover of extracellular matrix, basement membranes, microscopy 🔬
Lover of extracellular matrix, basement membranes, microscopy 🔬
i had a great time presenting my work at the American Society for Matrix Biology 2025 Biennial Meeting in Baltimore, MA!
As always, great to see fantastic developments within the community and make new connections!
As always, great to see fantastic developments within the community and make new connections!
November 20, 2025 at 1:15 PM
i had a great time presenting my work at the American Society for Matrix Biology 2025 Biennial Meeting in Baltimore, MA!
As always, great to see fantastic developments within the community and make new connections!
As always, great to see fantastic developments within the community and make new connections!
Reposted by Conor Sugden
The annual course “Basics in Fluorescence Microscopy”, will be organised by the van Leeuwenhoek Centre for Advanced Microscopy from January 27th - 30th, 2026.
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Basics in fluorescence microscopy – Microscopycourse
www.microscopycourse.nl
November 19, 2025 at 1:48 PM
The annual course “Basics in Fluorescence Microscopy”, will be organised by the van Leeuwenhoek Centre for Advanced Microscopy from January 27th - 30th, 2026.
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Reposted by Conor Sugden
Local #mRNAtranslation in polarized cells is well studied in neurons but what about #microglia? This study shows that #inflammation enhances microglial #RNA localization & translation via RNA-binding protein IMP1/ZBP1, regulating morphology, motility & phagocytosis @plosbiology.org 🧪 plos.io/486ZLMS
November 11, 2025 at 9:48 AM
Local #mRNAtranslation in polarized cells is well studied in neurons but what about #microglia? This study shows that #inflammation enhances microglial #RNA localization & translation via RNA-binding protein IMP1/ZBP1, regulating morphology, motility & phagocytosis @plosbiology.org 🧪 plos.io/486ZLMS
Reposted by Conor Sugden
The ECM is no longer in the back seat when it comes to development. Check out our special issue "Dynamics of the extracellular matrix in development, cell physiology and disease" with 8 selected articles to learn more: A 🧵 www.sciencedirect.com/special-issu...
Cells & Development | Dynamics of the extracellular matrix in development, cell physiology and disease. | ScienceDirect.com by Elsevier
Extracellular matrices (ECMs) are everywhere in our body– from the sheet-like basement membranes that line epithelia and other tissues - to the loose stromal arrays of mesenchyme - to the rigid struct...
www.sciencedirect.com
October 30, 2025 at 5:48 PM
The ECM is no longer in the back seat when it comes to development. Check out our special issue "Dynamics of the extracellular matrix in development, cell physiology and disease" with 8 selected articles to learn more: A 🧵 www.sciencedirect.com/special-issu...
Reposted by Conor Sugden
The IMAG'IC team is back to offer you the "Introduction to the Use of #ImageJ" training course.
October 24, 2025: Session in French
October 29, 2025: Session in English
institutcochin.fr/formations/f...
@institutcochin.bsky.social
@upcite.bsky.social
@inserm.fr
October 24, 2025: Session in French
October 29, 2025: Session in English
institutcochin.fr/formations/f...
@institutcochin.bsky.social
@upcite.bsky.social
@inserm.fr
October 6, 2025 at 3:07 PM
The IMAG'IC team is back to offer you the "Introduction to the Use of #ImageJ" training course.
October 24, 2025: Session in French
October 29, 2025: Session in English
institutcochin.fr/formations/f...
@institutcochin.bsky.social
@upcite.bsky.social
@inserm.fr
October 24, 2025: Session in French
October 29, 2025: Session in English
institutcochin.fr/formations/f...
@institutcochin.bsky.social
@upcite.bsky.social
@inserm.fr
Reposted by Conor Sugden
We're hiring new faculty! Looking for talented scientists that are interested in pushing the boundaries of Cell Biology. Please spread the word!
September 4, 2025 at 2:22 PM
We're hiring new faculty! Looking for talented scientists that are interested in pushing the boundaries of Cell Biology. Please spread the word!
Reposted by Conor Sugden
🔴✨ Exciting opportunity : we're hiring a PhD student! ✨🔴
👉 Don't hesitate to apply or spread the word!
⏰ Deadline 30th November 2025
ℹ️ More information and application : www.crg.eu/en/content/t...
@crg.eu @prbb.org
👉 Don't hesitate to apply or spread the word!
⏰ Deadline 30th November 2025
ℹ️ More information and application : www.crg.eu/en/content/t...
@crg.eu @prbb.org
Centre for Genomic Regulation Website
www.crg.eu
September 30, 2025 at 3:12 PM
🔴✨ Exciting opportunity : we're hiring a PhD student! ✨🔴
👉 Don't hesitate to apply or spread the word!
⏰ Deadline 30th November 2025
ℹ️ More information and application : www.crg.eu/en/content/t...
@crg.eu @prbb.org
👉 Don't hesitate to apply or spread the word!
⏰ Deadline 30th November 2025
ℹ️ More information and application : www.crg.eu/en/content/t...
@crg.eu @prbb.org
Reposted by Conor Sugden
[Commentary] Distal enhancers loop to proximal enhancers, not to promoters by the one and only Kevin Struhl in #genesky @natrevmcb.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Distal enhancers loop to proximal enhancers, not to promoters - Nature Reviews Molecular Cell Biology
This Comment posits that enhancers stimulate the transcription machinery only through short-range interactions and, therefore, that enhancer activity at a distance is mediated by chromatin loops betwe...
www.nature.com
September 9, 2025 at 9:56 AM
[Commentary] Distal enhancers loop to proximal enhancers, not to promoters by the one and only Kevin Struhl in #genesky @natrevmcb.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Conor Sugden
Super-Resolution Imaging Reveals Stretch-Induced Architectural Rearrangement of Desmoplakin in Desmosomes https://www.biorxiv.org/content/10.1101/2025.08.26.672431v1
August 27, 2025 at 9:31 PM
Super-Resolution Imaging Reveals Stretch-Induced Architectural Rearrangement of Desmoplakin in Desmosomes https://www.biorxiv.org/content/10.1101/2025.08.26.672431v1
Reposted by Conor Sugden
Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩
Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9 #protocol #starprotocols #cellpress
Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9
Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency. Here, we present a protocol for fast antibiotic resistance-based gene editing with CRISPR-Cas9 (FAB-CRISPR), which streamlines N/C-terminal tagging using an antibiotic resistance cassette for rapid selection and enrichment of gene-edited cells. We describe in detail guide RNA and HDR donor plasmid cloning, transfection of editin...
dlvr.it
August 13, 2025 at 10:22 AM
Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩
Reposted by Conor Sugden
Lentiviral vectors have stably integrated themselves as a favorite in the viral vector toolbox. In our recently refreshed lentiviral guide, we cover the basics of lentiviral vectors including generations, production, common uses, and safety. Check it out!
https://twp.ai/4ipGPa
https://twp.ai/4ipGPa
Addgene: Lentiviral Vector Guide
Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and common uses.
twp.ai
August 12, 2025 at 6:00 PM
Lentiviral vectors have stably integrated themselves as a favorite in the viral vector toolbox. In our recently refreshed lentiviral guide, we cover the basics of lentiviral vectors including generations, production, common uses, and safety. Check it out!
https://twp.ai/4ipGPa
https://twp.ai/4ipGPa
Reposted by Conor Sugden
Wonderful to be at the 15th International Podocyte Conference in Hamburg! Exciting science & fabulous networking ❤️
Huge 🙏 to @tobias-b-huber.bsky.social & @isgd.bsky.social teams 🙏
Huge 🙏 to @tobias-b-huber.bsky.social & @isgd.bsky.social teams 🙏
June 13, 2025 at 9:57 AM
Wonderful to be at the 15th International Podocyte Conference in Hamburg! Exciting science & fabulous networking ❤️
Huge 🙏 to @tobias-b-huber.bsky.social & @isgd.bsky.social teams 🙏
Huge 🙏 to @tobias-b-huber.bsky.social & @isgd.bsky.social teams 🙏
Reposted by Conor Sugden
🖱️ Picking out individual cells in distorted microscopy images is now as easy as clicking a button, thanks to Cellpose3: www.janelia.org/news/newest-...
Newest version of Cellpose can spot cell boundaries even in cloudy conditions
Picking out individual cells in distorted microscopy images is now as easy as clicking a button. A new version of Cellpose – the popular tool that maps the boundaries of diverse cells in microscopy
www.janelia.org
May 9, 2025 at 1:22 PM
🖱️ Picking out individual cells in distorted microscopy images is now as easy as clicking a button, thanks to Cellpose3: www.janelia.org/news/newest-...
Reposted by Conor Sugden
Amazing postdoctoral position at Pernaute’s lab
Fully funded for 3 year by European Research Council (#ERC) grant EARLYFATE #ERCCoG
At the #CABD a research center recognized with a national excellence award ‘María de Maeztu’ located in Seville, Spain
Share or apply
#JobOffer
#Postdoctoraljob
Fully funded for 3 year by European Research Council (#ERC) grant EARLYFATE #ERCCoG
At the #CABD a research center recognized with a national excellence award ‘María de Maeztu’ located in Seville, Spain
Share or apply
#JobOffer
#Postdoctoraljob
🚨🚨#Hiring!
3 year #postdoc position open in my lab @cabd-upo-csic.bsky.social to study the interplay between DNA damage responses and #preimplantation embryo development. Fully funded by EARLYFATE #ERCCoG
📆 Apply by 23rd May 2023
🔗Details below
@sebioldev.bsky.social
Please RT!🙏
3 year #postdoc position open in my lab @cabd-upo-csic.bsky.social to study the interplay between DNA damage responses and #preimplantation embryo development. Fully funded by EARLYFATE #ERCCoG
📆 Apply by 23rd May 2023
🔗Details below
@sebioldev.bsky.social
Please RT!🙏
April 23, 2025 at 7:44 PM
Amazing postdoctoral position at Pernaute’s lab
Fully funded for 3 year by European Research Council (#ERC) grant EARLYFATE #ERCCoG
At the #CABD a research center recognized with a national excellence award ‘María de Maeztu’ located in Seville, Spain
Share or apply
#JobOffer
#Postdoctoraljob
Fully funded for 3 year by European Research Council (#ERC) grant EARLYFATE #ERCCoG
At the #CABD a research center recognized with a national excellence award ‘María de Maeztu’ located in Seville, Spain
Share or apply
#JobOffer
#Postdoctoraljob
Reposted by Conor Sugden
For clear thinking, cultivate passionate indifference. Buddhists call it non-attachment. We all have hopes, desires and ambitions. Non-attachment means acknowledging them, accepting them, and then not inserting them into a process that has nothing to do with you. podcasts.apple.com/us/podcast/n...
April 22, 2025 at 3:03 PM
For clear thinking, cultivate passionate indifference. Buddhists call it non-attachment. We all have hopes, desires and ambitions. Non-attachment means acknowledging them, accepting them, and then not inserting them into a process that has nothing to do with you. podcasts.apple.com/us/podcast/n...
Reposted by Conor Sugden
On April 19, 1898, Camillo Golgi described what we now know as the #Golgi apparatus - a structure so pretty and unusual it took over fifty years for the scientific community to agree it wasn’t just an artefact.
April 19 is #GolgiDay! Mark your calendars to honour the best organelle.
April 19 is #GolgiDay! Mark your calendars to honour the best organelle.
April 16, 2025 at 7:39 PM
Reposted by Conor Sugden
Here we share our opinions on "Practical considerations for data exploration in quantitative cell biology"
journals.biologists.com/jcs/article/...
Joanna, Hanna and I worked quite a bit on this piece and I hope you find it useful
Many thanks to @jcellsci.bsky.social for publishing it
journals.biologists.com/jcs/article/...
Joanna, Hanna and I worked quite a bit on this piece and I hope you find it useful
Many thanks to @jcellsci.bsky.social for publishing it
Practical considerations for data exploration in quantitative cell biology
Summary: We present practical guidelines for data exploration in quantitative cell biology that are designed to help yield reliable conclusions and promote a collaborative, transparent approach to dat...
journals.biologists.com
April 7, 2025 at 9:15 AM
Here we share our opinions on "Practical considerations for data exploration in quantitative cell biology"
journals.biologists.com/jcs/article/...
Joanna, Hanna and I worked quite a bit on this piece and I hope you find it useful
Many thanks to @jcellsci.bsky.social for publishing it
journals.biologists.com/jcs/article/...
Joanna, Hanna and I worked quite a bit on this piece and I hope you find it useful
Many thanks to @jcellsci.bsky.social for publishing it
Reposted by Conor Sugden
Contrary to popular belief, what is important in science is as much its spirit as its product: it is as much the openmindedness, the primacy of criticism, the submission to the unforeseen, however upsetting, as the result, however new that may be. – Francois Jacob
www.nature.com/articles/s41...
www.nature.com/articles/s41...
April 7, 2025 at 1:07 AM
Contrary to popular belief, what is important in science is as much its spirit as its product: it is as much the openmindedness, the primacy of criticism, the submission to the unforeseen, however upsetting, as the result, however new that may be. – Francois Jacob
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Conor Sugden
Join the biocontrol seminar tomorrow to hear about the fun things we are doing with controlling cells!
March 24, 2025 at 4:55 PM
Join the biocontrol seminar tomorrow to hear about the fun things we are doing with controlling cells!
Reposted by Conor Sugden
This will never stop blowing my mind.
I know that the mRNA is larger than the protein it codes for but I don't believe it
March 20, 2025 at 12:31 AM
This will never stop blowing my mind.
Reposted by Conor Sugden
Looking for #MaterialStiffness of #BasementMembrane across tissues?
Excellent literature resource here😆👇
The basement membrane as a structured surface – role in vascular health and disease
@claireleclech.bsky.social @jcellsci.bsky.social 2020
journals.biologists.com/jcs/article/...
Excellent literature resource here😆👇
The basement membrane as a structured surface – role in vascular health and disease
@claireleclech.bsky.social @jcellsci.bsky.social 2020
journals.biologists.com/jcs/article/...
March 7, 2025 at 12:51 PM
Looking for #MaterialStiffness of #BasementMembrane across tissues?
Excellent literature resource here😆👇
The basement membrane as a structured surface – role in vascular health and disease
@claireleclech.bsky.social @jcellsci.bsky.social 2020
journals.biologists.com/jcs/article/...
Excellent literature resource here😆👇
The basement membrane as a structured surface – role in vascular health and disease
@claireleclech.bsky.social @jcellsci.bsky.social 2020
journals.biologists.com/jcs/article/...
Reposted by Conor Sugden
A terrific perspective from Vivek Malhotra @naturecomms.bsky.social on the fundamental importance of pathways and mechanisms by which cells secrete proteins, ensuring their delivery in the correct quantities and at the appropriate times.
Interesting section on unanswered questions.
rdcu.be/ecb2B
Interesting section on unanswered questions.
rdcu.be/ecb2B
The pathways of secretory cargo export at the endoplasmic reticulum
Nature Communications - The author presents an overview of the complex challenge of protein export from the Endoplasmic Reticulum, and discusses how cells may deploy novel pathways utilizing...
rdcu.be
March 4, 2025 at 10:00 PM
A terrific perspective from Vivek Malhotra @naturecomms.bsky.social on the fundamental importance of pathways and mechanisms by which cells secrete proteins, ensuring their delivery in the correct quantities and at the appropriate times.
Interesting section on unanswered questions.
rdcu.be/ecb2B
Interesting section on unanswered questions.
rdcu.be/ecb2B
Reposted by Conor Sugden
Rhobo6👹
An omnipotent cell-impermeable dye labels diol/glycan of #ExtraCellularMatrix
✅collagen/fibron, BM, proteoglycan
Retro-orbital injection to label the ECM throughout WHOLE mouse body▶️intravital 2P
Also work on🪰🪱🐟🌱
@naturemethods.bsky.social 2025
www.nature.com/articles/s41...
An omnipotent cell-impermeable dye labels diol/glycan of #ExtraCellularMatrix
✅collagen/fibron, BM, proteoglycan
Retro-orbital injection to label the ECM throughout WHOLE mouse body▶️intravital 2P
Also work on🪰🪱🐟🌱
@naturemethods.bsky.social 2025
www.nature.com/articles/s41...
March 1, 2025 at 12:16 PM
Rhobo6👹
An omnipotent cell-impermeable dye labels diol/glycan of #ExtraCellularMatrix
✅collagen/fibron, BM, proteoglycan
Retro-orbital injection to label the ECM throughout WHOLE mouse body▶️intravital 2P
Also work on🪰🪱🐟🌱
@naturemethods.bsky.social 2025
www.nature.com/articles/s41...
An omnipotent cell-impermeable dye labels diol/glycan of #ExtraCellularMatrix
✅collagen/fibron, BM, proteoglycan
Retro-orbital injection to label the ECM throughout WHOLE mouse body▶️intravital 2P
Also work on🪰🪱🐟🌱
@naturemethods.bsky.social 2025
www.nature.com/articles/s41...
Reposted by Conor Sugden
🔬✨ SCIENCE THAT GLOWS💡🔴
Conor Sugden, Shota Kaseda, Sherwood Lab & UoM’s Genome Editing Unit made basement membranes glow red with mScarlet. This helps scientists see damage & repair in real-time—leading to better treatments! 🏥💙
@conorjsugden.bsky.social
@rachellennon.bsky.social
Conor Sugden, Shota Kaseda, Sherwood Lab & UoM’s Genome Editing Unit made basement membranes glow red with mScarlet. This helps scientists see damage & repair in real-time—leading to better treatments! 🏥💙
@conorjsugden.bsky.social
@rachellennon.bsky.social
February 26, 2025 at 1:42 PM
🔬✨ SCIENCE THAT GLOWS💡🔴
Conor Sugden, Shota Kaseda, Sherwood Lab & UoM’s Genome Editing Unit made basement membranes glow red with mScarlet. This helps scientists see damage & repair in real-time—leading to better treatments! 🏥💙
@conorjsugden.bsky.social
@rachellennon.bsky.social
Conor Sugden, Shota Kaseda, Sherwood Lab & UoM’s Genome Editing Unit made basement membranes glow red with mScarlet. This helps scientists see damage & repair in real-time—leading to better treatments! 🏥💙
@conorjsugden.bsky.social
@rachellennon.bsky.social
Reposted by Conor Sugden
🔔🧪Let’s support our colleagues:
👉“The upcoming weeks will challenge the US #scientific #community, emphasizing the need for #unity and #support among its members. Upholding principles of #independence, #peer evaluation, and #inclusion is vital for future #success.”
www.science.org/doi/10.1126/...
👉“The upcoming weeks will challenge the US #scientific #community, emphasizing the need for #unity and #support among its members. Upholding principles of #independence, #peer evaluation, and #inclusion is vital for future #success.”
www.science.org/doi/10.1126/...
Come together, right now
The chaos, conflicting information, firings, and hurtful rhetoric of the Trump administration’s approach to science over the past month are causing anxiety, grief, and concern for the scientific commu...
www.science.org
February 25, 2025 at 7:15 PM
🔔🧪Let’s support our colleagues:
👉“The upcoming weeks will challenge the US #scientific #community, emphasizing the need for #unity and #support among its members. Upholding principles of #independence, #peer evaluation, and #inclusion is vital for future #success.”
www.science.org/doi/10.1126/...
👉“The upcoming weeks will challenge the US #scientific #community, emphasizing the need for #unity and #support among its members. Upholding principles of #independence, #peer evaluation, and #inclusion is vital for future #success.”
www.science.org/doi/10.1126/...