Tim Feinstein
@timnfeinstein.bsky.social
Ph.D. ex-academic, self-published author of “A Heuristic Guide To Quantitative Imaging”. Lead, operations and technical sales at Coastal Microscopes.
And then single blind it so the doctors running the study, assuming they are not already biased, know exactly how to give the funding source the results it asked for.
It is unethical to do a randomized controlled trial in which you withhold a proven, life-saving vaccine from newborn babies.
December 18, 2025 at 1:14 PM
And then single blind it so the doctors running the study, assuming they are not already biased, know exactly how to give the funding source the results it asked for.
Someone finally read a book about Rome.
Possible answer: the supplemental DOD funds in the earlier reconciliation had few strings attached and included several billion intended for housing allowances which DOD says it is tapping www.defenseone.com/policy/2025/...
December 18, 2025 at 12:28 PM
Someone finally read a book about Rome.
Reposted by Tim Feinstein
We have a ✨new website✨ - go and take a look to meet our team, find out how you can work with us, and see our upcoming training events!
www.crick.ac.uk/research/pla...
www.crick.ac.uk/research/pla...
Image Analysis
The golden rule of image analysis is “garbage in, garbage out”, so we strongly encourage researchers to come and talk to us about their experimental plans before acquiring data.
www.crick.ac.uk
December 12, 2025 at 11:00 AM
We have a ✨new website✨ - go and take a look to meet our team, find out how you can work with us, and see our upcoming training events!
www.crick.ac.uk/research/pla...
www.crick.ac.uk/research/pla...
>:^|
Grading and googling hallucinated citations, as one does nowadays, and now that LLMs have been around for a while, I've discovered new horrors: hallucinated journals are now appearing in Google Scholar with dozens of citations bc so many people are citing these fake things
December 16, 2025 at 12:43 PM
>:^|
Reposted by Tim Feinstein
Getting SMART about FRAP enabled us to make many FRAP curves in one imaging session. Now we are doing things like 18 conditions n = 30 in a single overnight session.
Study protein dynamics in high throughput.
Curious on how? see our publication:
febs.onlinelibrary.wiley.com/doi/full/10....
Study protein dynamics in high throughput.
Curious on how? see our publication:
febs.onlinelibrary.wiley.com/doi/full/10....
FEBS Press
RoboMic is an automated confocal microscopy pipeline for high-throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI-driv...
febs.onlinelibrary.wiley.com
December 16, 2025 at 9:53 AM
Getting SMART about FRAP enabled us to make many FRAP curves in one imaging session. Now we are doing things like 18 conditions n = 30 in a single overnight session.
Study protein dynamics in high throughput.
Curious on how? see our publication:
febs.onlinelibrary.wiley.com/doi/full/10....
Study protein dynamics in high throughput.
Curious on how? see our publication:
febs.onlinelibrary.wiley.com/doi/full/10....
Reposted by Tim Feinstein
Woop Woop, it's out!!! Congratulations everyone. A beautiful paper
December 15, 2025 at 2:52 PM
Woop Woop, it's out!!! Congratulations everyone. A beautiful paper
Reposted by Tim Feinstein
From paleoart to fine art,
comics to medical animation,
bioart to space imagery,
explore the rich SciArt ecosystem on Bluesky with the SciArt Starter Pack.
150 artists, plus the entire popular SciArt Feed 🐡 to pin to your skyline.
And no gen AI or NFTs. 🥳
comics to medical animation,
bioart to space imagery,
explore the rich SciArt ecosystem on Bluesky with the SciArt Starter Pack.
150 artists, plus the entire popular SciArt Feed 🐡 to pin to your skyline.
And no gen AI or NFTs. 🥳
December 11, 2025 at 3:48 PM
From paleoart to fine art,
comics to medical animation,
bioart to space imagery,
explore the rich SciArt ecosystem on Bluesky with the SciArt Starter Pack.
150 artists, plus the entire popular SciArt Feed 🐡 to pin to your skyline.
And no gen AI or NFTs. 🥳
comics to medical animation,
bioart to space imagery,
explore the rich SciArt ecosystem on Bluesky with the SciArt Starter Pack.
150 artists, plus the entire popular SciArt Feed 🐡 to pin to your skyline.
And no gen AI or NFTs. 🥳
Gozer: CHOOSE the form of your [AI] destructor
Venkman: Nobody choose anything!
Ray: I couldn’t help it
Venkman: Nobody choose anything!
Ray: I couldn’t help it
December 12, 2025 at 3:42 PM
Gozer: CHOOSE the form of your [AI] destructor
Venkman: Nobody choose anything!
Ray: I couldn’t help it
Venkman: Nobody choose anything!
Ray: I couldn’t help it
December 4, 2025 at 2:38 AM
Reposted by Tim Feinstein
Hey folks in Dresden, I have a very good friend that is a microscopy expert there in a bit of a tight spot - anyone have any openings ?
DM me!
DM me!
a close up of a feather on a rock with the words `` hello '' written in white letters .
ALT: a close up of a feather on a rock with the words `` hello '' written in white letters .
media.tenor.com
December 2, 2025 at 1:20 PM
Hey folks in Dresden, I have a very good friend that is a microscopy expert there in a bit of a tight spot - anyone have any openings ?
DM me!
DM me!
Reposted by Tim Feinstein
That’s the point.
LLM owners want exclusivity for institutional expertise, among other things, so they can charge everyone who wants to access it.
Wikipedia is one of their most hated foes since it provides free knowledge to the masses. And everyone should be supporting Wikipedia for that reason.
LLM owners want exclusivity for institutional expertise, among other things, so they can charge everyone who wants to access it.
Wikipedia is one of their most hated foes since it provides free knowledge to the masses. And everyone should be supporting Wikipedia for that reason.
November 30, 2025 at 5:43 PM
That’s the point.
LLM owners want exclusivity for institutional expertise, among other things, so they can charge everyone who wants to access it.
Wikipedia is one of their most hated foes since it provides free knowledge to the masses. And everyone should be supporting Wikipedia for that reason.
LLM owners want exclusivity for institutional expertise, among other things, so they can charge everyone who wants to access it.
Wikipedia is one of their most hated foes since it provides free knowledge to the masses. And everyone should be supporting Wikipedia for that reason.
“…malware that can use large language models to write custom code for each hacking attempt”
That’s basically a digital version of Stephen King’s flu from The Stand.
That’s basically a digital version of Stephen King’s flu from The Stand.
November 26, 2025 at 12:50 PM
“…malware that can use large language models to write custom code for each hacking attempt”
That’s basically a digital version of Stephen King’s flu from The Stand.
That’s basically a digital version of Stephen King’s flu from The Stand.
Reposted by Tim Feinstein
I’ve thought a lot about this problem. Yes, AI garbage will soon be near impossible to distinguish from real microscope images.
You could probably still use a decon-like PSF model to flag raw image data that doesn’t follow known optical physics. $10 says they’re talking about it at JCB.
1/4
You could probably still use a decon-like PSF model to flag raw image data that doesn’t follow known optical physics. $10 says they’re talking about it at JCB.
1/4
I tried an even harder example on Gemini Pro image generation and this is quite scary/amazing. I asked for a microscopy image of around 20 HeLa cells, GFP tagged 20% nuclear, 10% membrane, +1 nuclear staining, + overlap. Image below and prompt in the following post.
November 23, 2025 at 12:13 PM
I’ve thought a lot about this problem. Yes, AI garbage will soon be near impossible to distinguish from real microscope images.
You could probably still use a decon-like PSF model to flag raw image data that doesn’t follow known optical physics. $10 says they’re talking about it at JCB.
1/4
You could probably still use a decon-like PSF model to flag raw image data that doesn’t follow known optical physics. $10 says they’re talking about it at JCB.
1/4
I’ve thought a lot about this problem. Yes, AI garbage will soon be near impossible to distinguish from real microscope images.
You could probably still use a decon-like PSF model to flag raw image data that doesn’t follow known optical physics. $10 says they’re talking about it at JCB.
1/4
You could probably still use a decon-like PSF model to flag raw image data that doesn’t follow known optical physics. $10 says they’re talking about it at JCB.
1/4
I tried an even harder example on Gemini Pro image generation and this is quite scary/amazing. I asked for a microscopy image of around 20 HeLa cells, GFP tagged 20% nuclear, 10% membrane, +1 nuclear staining, + overlap. Image below and prompt in the following post.
November 23, 2025 at 12:13 PM
I’ve thought a lot about this problem. Yes, AI garbage will soon be near impossible to distinguish from real microscope images.
You could probably still use a decon-like PSF model to flag raw image data that doesn’t follow known optical physics. $10 says they’re talking about it at JCB.
1/4
You could probably still use a decon-like PSF model to flag raw image data that doesn’t follow known optical physics. $10 says they’re talking about it at JCB.
1/4
For people old enough to remember, a big inflection point for the NY Times happened when Sulzberger chose to go full USA hoo-rah after 9/11, at the expense of integrity & standards. The Judith Miller saga just capped off a long, sad slide. Willing coordination w neocon agitprop got pretty bad.
November 13, 2025 at 8:58 AM
For people old enough to remember, a big inflection point for the NY Times happened when Sulzberger chose to go full USA hoo-rah after 9/11, at the expense of integrity & standards. The Judith Miller saga just capped off a long, sad slide. Willing coordination w neocon agitprop got pretty bad.
Reposted by Tim Feinstein
We are looking for a PhD student to work on an exciting plastid endosymbiosis in microbial eukaryotes. This position involves sampling, exciting microscopy such as CARDFISH, ExM and FIBSEM, single-cell transcriptomics and more. #protistsonsky 1/2
November 12, 2025 at 9:50 AM
We are looking for a PhD student to work on an exciting plastid endosymbiosis in microbial eukaryotes. This position involves sampling, exciting microscopy such as CARDFISH, ExM and FIBSEM, single-cell transcriptomics and more. #protistsonsky 1/2
Reposted by Tim Feinstein
My book 'The Tree of Life' is published in the USA and Canada today.
Available as book, on kindle and as audio.
I would be really grateful for reposts.
www.amazon.com/Tree-Life-So...
www.amazon.ca/Tree-Life-So...
Available as book, on kindle and as audio.
I would be really grateful for reposts.
www.amazon.com/Tree-Life-So...
www.amazon.ca/Tree-Life-So...
November 11, 2025 at 12:01 PM
My book 'The Tree of Life' is published in the USA and Canada today.
Available as book, on kindle and as audio.
I would be really grateful for reposts.
www.amazon.com/Tree-Life-So...
www.amazon.ca/Tree-Life-So...
Available as book, on kindle and as audio.
I would be really grateful for reposts.
www.amazon.com/Tree-Life-So...
www.amazon.ca/Tree-Life-So...
Reposted by Tim Feinstein
Okay, it is still open enrollment and i am still trying to convince you to go and sign up for health insurance. I know it is expensive and scary. But you can do it. The thread embedded here is a tutorial on how to understand and make that decision. I will now write a quickie version.
It's open enrollment time so here's a convenient peg to hang a little tutorial about how to choose your health insurance.
Most of the advice you get is bad. Most of your instincts are bad. I however used to work for a health insurance company and I do arithmetic for a living so I can help.
Most of the advice you get is bad. Most of your instincts are bad. I however used to work for a health insurance company and I do arithmetic for a living so I can help.
Personally I think it's great that my company's HR sends out an official looking tool where they even ask you to pre-populate your healthcare providers and instead of being the actual insurance enrollment process it's just some bullshit checklist to try and convince me I actually don't want a PPO
November 9, 2025 at 9:10 PM
Okay, it is still open enrollment and i am still trying to convince you to go and sign up for health insurance. I know it is expensive and scary. But you can do it. The thread embedded here is a tutorial on how to understand and make that decision. I will now write a quickie version.
Our 12yo determined that her current favorite animal, the jerboa, go on our pumpkin this year. But talk about a cool Golgi pumpkin! Following convention, it looks like cis- is down.
November 8, 2025 at 9:27 PM
Our 12yo determined that her current favorite animal, the jerboa, go on our pumpkin this year. But talk about a cool Golgi pumpkin! Following convention, it looks like cis- is down.
Not looking to disrupt anyone’s day, but a whole new endomembrane system just dropped. What does that stuff do? No one knows yet!
We have wondered what a complex archaeal cell might look like ever since 2014. It’s been a long road (and the journey is far from over), but it’s a good time to pause for breath and look. These Asgard archaeal cells are a surprise! And that is the joy of being a cell biologist.
November 8, 2025 at 7:43 PM
Not looking to disrupt anyone’s day, but a whole new endomembrane system just dropped. What does that stuff do? No one knows yet!
Does anyone know if Democritus actually wrote a book about chameleons? This might be kind of niche.
November 8, 2025 at 1:32 AM
Does anyone know if Democritus actually wrote a book about chameleons? This might be kind of niche.
Reposted by Tim Feinstein
Required reading for cell biologists to get a sense of basic statistical principles!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Ten essential tips for robust statistics in cell biology - Nature Cell Biology
Statistical thinking is a core part of solid, trustworthy biology. However, many studies still include insufficient sample sizes, have poor experimental design or select an incorrect statistical metho...
www.nature.com
November 4, 2025 at 7:23 PM
Required reading for cell biologists to get a sense of basic statistical principles!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Tim Feinstein
My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
November 4, 2025 at 10:40 PM
My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
Reposted by Tim Feinstein
Registration closes Friday at noon! Are you in South-Western Ontario and interested in fluorescence microscopy? If so, we are offering a 2-day course on designing, implementing and analysing microscopy experiments. Visit our website for more information: micrographiauwo.ca/cc2025/ #microscopy
October 29, 2025 at 11:22 AM
Registration closes Friday at noon! Are you in South-Western Ontario and interested in fluorescence microscopy? If so, we are offering a 2-day course on designing, implementing and analysing microscopy experiments. Visit our website for more information: micrographiauwo.ca/cc2025/ #microscopy