Juanma García-Arcos
@juanmagararc.bsky.social
Group leader at EPFL, Lausanne. Formerly, Postdoc Roux lab, Geneva; PhD Piel lab, Paris. #cytoplasts #actomyosin #cellbio #microscopy #membranes #biophysics #extracellular_vesicles #sciart
Lab website: http://celldynamicslab.com
Lab website: http://celldynamicslab.com
Pinned
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM
Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...
www.biorxiv.org
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
Reposted by Juanma García-Arcos
Felicidades querido JuanMa !
El Breaking lab, o el lab FLIPado (de FLIPR) será enorme...
El Breaking lab, o el lab FLIPado (de FLIPR) será enorme...
elmo from sesame street is surrounded by flames and smoke
ALT: elmo from sesame street is surrounded by flames and smoke
media.tenor.com
November 15, 2025 at 6:50 PM
Felicidades querido JuanMa !
El Breaking lab, o el lab FLIPado (de FLIPR) será enorme...
El Breaking lab, o el lab FLIPado (de FLIPR) será enorme...
Reposted by Juanma García-Arcos
Maria Tettamanti shows that inhibition of TORC2 by the small amphipath PalmC induces the TORC2-dependent internalization of sterols. Congrats to Maria and all coworkers involved, and to EMBOj for the efficient review process: use review commons! @biology-unige.bsky.social @sciencesunige.bsky.social
Yeast cells utilize a TORC2 feedback loop for plasma membrane adaptation to mechanical stress, with increased sterol transport from plasma membrane to endoplasmic reticulum activating TORC2 signaling
Robbie Loewith, Aurélien Roux @rouxlab.bsky.social and coworkers
www.embopress.org/doi/full/10....
Robbie Loewith, Aurélien Roux @rouxlab.bsky.social and coworkers
www.embopress.org/doi/full/10....
November 14, 2025 at 10:35 AM
Maria Tettamanti shows that inhibition of TORC2 by the small amphipath PalmC induces the TORC2-dependent internalization of sterols. Congrats to Maria and all coworkers involved, and to EMBOj for the efficient review process: use review commons! @biology-unige.bsky.social @sciencesunige.bsky.social
Launching celldynamicslab.com, the homepage of our new group at EPFL working on cell fragmentation, membrane and cortex mechanics, FLIM imaging, and microfluidics tools. MSc/PhD or postdocs interested in quantitative cell biology are welcome to reach out. We're also hiring a lab manager in 2026!
Main - Cell Dynamics and Fragmentation Lab
The Cell Dynamics and Fragmentation lab at We study how single cells move and fragment in complex environments using microfluidics. We bring tools and concep...
celldynamicslab.com
November 11, 2025 at 5:10 PM
Launching celldynamicslab.com, the homepage of our new group at EPFL working on cell fragmentation, membrane and cortex mechanics, FLIM imaging, and microfluidics tools. MSc/PhD or postdocs interested in quantitative cell biology are welcome to reach out. We're also hiring a lab manager in 2026!
Reposted by Juanma García-Arcos
Actin dynamics sustains spatial gradients of membrane tension in adherent cells https://www.biorxiv.org/content/10.1101/2024.07.15.603517v1
Actin dynamics sustains spatial gradients of membrane tension in adherent cells https://www.biorxiv.org/content/10.1101/2024.07.15.603517v1
Tension propagates extremely fast in lipid bilayers, precluding the formation of tension gradients.
www.biorxiv.org
July 16, 2024 at 5:30 PM
Actin dynamics sustains spatial gradients of membrane tension in adherent cells https://www.biorxiv.org/content/10.1101/2024.07.15.603517v1
Reposted by Juanma García-Arcos
Super moon. 8”x6” oil
November 9, 2025 at 4:48 PM
Super moon. 8”x6” oil
Reposted by Juanma García-Arcos
Emerging trends of fluorescence lifetime imaging microscopy (FLIM): advances, challenges, and prospects
www.biophysics-reports.org/article/doi/...
www.biophysics-reports.org/article/doi/...
November 3, 2025 at 11:24 AM
Emerging trends of fluorescence lifetime imaging microscopy (FLIM): advances, challenges, and prospects
www.biophysics-reports.org/article/doi/...
www.biophysics-reports.org/article/doi/...
Reposted by Juanma García-Arcos
Check the new preprint of @juanmagararc.bsky.social !
All you wanted to know about FLIPER and all the things you need to take in account before doing a FLIPER-TR experiment :)
All you wanted to know about FLIPER and all the things you need to take in account before doing a FLIPER-TR experiment :)
October 15, 2025 at 1:24 PM
Check the new preprint of @juanmagararc.bsky.social !
All you wanted to know about FLIPER and all the things you need to take in account before doing a FLIPER-TR experiment :)
All you wanted to know about FLIPER and all the things you need to take in account before doing a FLIPER-TR experiment :)
Reposted by Juanma García-Arcos
If you are planning to do Flipper-TR experiments or FLIM imaging in general, I strongly recommend reading this beautiful method paper first! Lots of information about optimal data acquisition for reliable data analysis! Nice work from Tithi, @rouxlab.bsky.social and @juanmagararc.bsky.social !
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM
Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...
www.biorxiv.org
October 22, 2025 at 8:54 AM
If you are planning to do Flipper-TR experiments or FLIM imaging in general, I strongly recommend reading this beautiful method paper first! Lots of information about optimal data acquisition for reliable data analysis! Nice work from Tithi, @rouxlab.bsky.social and @juanmagararc.bsky.social !
Reposted by Juanma García-Arcos
All you've ever wanted to know about FLIM analysis (and its application in Flipper-TR)!😱🤩🔬
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM
Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...
www.biorxiv.org
October 7, 2025 at 1:54 PM
All you've ever wanted to know about FLIM analysis (and its application in Flipper-TR)!😱🤩🔬
Reposted by Juanma García-Arcos
An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM
Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...
www.biorxiv.org
October 7, 2025 at 8:21 AM
An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
Reposted by Juanma García-Arcos
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM by Juan Manuel Garcia-Arcos and team: www.biorxiv.org/content/10.1...
September 26, 2025 at 1:29 PM
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM by Juan Manuel Garcia-Arcos and team: www.biorxiv.org/content/10.1...
Reposted by Juanma García-Arcos
Dr. Jane Goodall filmed an interview with Netflix in March 2025 that she understood would only be released after her death.
October 5, 2025 at 9:08 AM
Dr. Jane Goodall filmed an interview with Netflix in March 2025 that she understood would only be released after her death.
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM
Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...
www.biorxiv.org
October 5, 2025 at 4:04 PM
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
Reposted by Juanma García-Arcos
📢New Actin Probe available at Spirochrome!
Introducing SPY650-FastAct_X our latest actin probe with highly improved kinetics and no toxicity!
spirochrome.com/product/spy6...
The product is now available from our distributors
Preprint from the @kjohnsson.bsky.social lab: doi.org/10.1101/2025...
Introducing SPY650-FastAct_X our latest actin probe with highly improved kinetics and no toxicity!
spirochrome.com/product/spy6...
The product is now available from our distributors
Preprint from the @kjohnsson.bsky.social lab: doi.org/10.1101/2025...
February 6, 2025 at 11:45 AM
📢New Actin Probe available at Spirochrome!
Introducing SPY650-FastAct_X our latest actin probe with highly improved kinetics and no toxicity!
spirochrome.com/product/spy6...
The product is now available from our distributors
Preprint from the @kjohnsson.bsky.social lab: doi.org/10.1101/2025...
Introducing SPY650-FastAct_X our latest actin probe with highly improved kinetics and no toxicity!
spirochrome.com/product/spy6...
The product is now available from our distributors
Preprint from the @kjohnsson.bsky.social lab: doi.org/10.1101/2025...
Reposted by Juanma García-Arcos
How cells decide where to go? #CellMigration
Last year we published our 1st paper @cellcommlab.bsky.social, showing how actin-based membrane oscillations define a new direction ( #cancer & non-cancer #cells )
Full www.nature.com/articles/s41...
@naturephysics.bsky.social @focalplane.bsky.social 🧪🔬
Last year we published our 1st paper @cellcommlab.bsky.social, showing how actin-based membrane oscillations define a new direction ( #cancer & non-cancer #cells )
Full www.nature.com/articles/s41...
@naturephysics.bsky.social @focalplane.bsky.social 🧪🔬
January 18, 2025 at 11:24 PM
How cells decide where to go? #CellMigration
Last year we published our 1st paper @cellcommlab.bsky.social, showing how actin-based membrane oscillations define a new direction ( #cancer & non-cancer #cells )
Full www.nature.com/articles/s41...
@naturephysics.bsky.social @focalplane.bsky.social 🧪🔬
Last year we published our 1st paper @cellcommlab.bsky.social, showing how actin-based membrane oscillations define a new direction ( #cancer & non-cancer #cells )
Full www.nature.com/articles/s41...
@naturephysics.bsky.social @focalplane.bsky.social 🧪🔬
Reposted by Juanma García-Arcos
#CellMigration happens all the time in our bodies. This may (or not) rely on adhesion molecules
#Cancer cells switch strategies during metastasis to invade other tissues
Naomi Wee (naomiwee.com) did this neat movie to explain it:
www.youtube.com/watch?v=D9aD...
#SciSky 🧪🔬 @cellcommlab.bsky.social
#Cancer cells switch strategies during metastasis to invade other tissues
Naomi Wee (naomiwee.com) did this neat movie to explain it:
www.youtube.com/watch?v=D9aD...
#SciSky 🧪🔬 @cellcommlab.bsky.social
February 6, 2025 at 10:44 AM
#CellMigration happens all the time in our bodies. This may (or not) rely on adhesion molecules
#Cancer cells switch strategies during metastasis to invade other tissues
Naomi Wee (naomiwee.com) did this neat movie to explain it:
www.youtube.com/watch?v=D9aD...
#SciSky 🧪🔬 @cellcommlab.bsky.social
#Cancer cells switch strategies during metastasis to invade other tissues
Naomi Wee (naomiwee.com) did this neat movie to explain it:
www.youtube.com/watch?v=D9aD...
#SciSky 🧪🔬 @cellcommlab.bsky.social
Reposted by Juanma García-Arcos
New preprint! When and how do active flows become turbulent? We found that, in unconfined active nematics, the transition to turbulence is discontinuous! With @the-chaotician.bsky.social @mpipks.bsky.social
arxiv.org/abs/2501.060...
arxiv.org/abs/2501.060...
January 15, 2025 at 5:22 PM
New preprint! When and how do active flows become turbulent? We found that, in unconfined active nematics, the transition to turbulence is discontinuous! With @the-chaotician.bsky.social @mpipks.bsky.social
arxiv.org/abs/2501.060...
arxiv.org/abs/2501.060...
Reposted by Juanma García-Arcos
I mostly see the protein sculptures I build in the lighting of my workshop. It’s fairly static and dull lighting. When they are close to being finished, I bring them into the house and enjoy watching how they change under different natural lighting throughout the day. They are so much more dynamic!
November 23, 2024 at 1:45 PM
I mostly see the protein sculptures I build in the lighting of my workshop. It’s fairly static and dull lighting. When they are close to being finished, I bring them into the house and enjoy watching how they change under different natural lighting throughout the day. They are so much more dynamic!
Reposted by Juanma García-Arcos
Would you prefer to lead a lab alone or together with a science buddy? How interesting would be a research institute where joint labs are the standard?!
November 22, 2024 at 11:42 PM
Would you prefer to lead a lab alone or together with a science buddy? How interesting would be a research institute where joint labs are the standard?!
Reposted by Juanma García-Arcos
i might be biased, but this place is amazing: great colleagues, vibrant campus, brilliant students/postdocs - and Vienna! perfect for starting an ambitious project. apply now for a GL position at the IMP …
www.imp.ac.at/career/open-...
www.imp.ac.at/career/open-...
Group leader positions at the IMP | Research Institute of Molecular Pathology
www.imp.ac.at
November 19, 2024 at 5:30 PM
i might be biased, but this place is amazing: great colleagues, vibrant campus, brilliant students/postdocs - and Vienna! perfect for starting an ambitious project. apply now for a GL position at the IMP …
www.imp.ac.at/career/open-...
www.imp.ac.at/career/open-...
Reposted by Juanma García-Arcos
Ok, trying to bring my community back together... I created a starter pack for lipids and membrane protein aficionados!
Feel free to share, add and distribute, so we can come together again! 😍
go.bsky.app/AEeXh86
Feel free to share, add and distribute, so we can come together again! 😍
go.bsky.app/AEeXh86
November 16, 2024 at 3:53 AM
Ok, trying to bring my community back together... I created a starter pack for lipids and membrane protein aficionados!
Feel free to share, add and distribute, so we can come together again! 😍
go.bsky.app/AEeXh86
Feel free to share, add and distribute, so we can come together again! 😍
go.bsky.app/AEeXh86
Reposted by Juanma García-Arcos
I started a list of people working broadly on cell adhesion and cell migration. I likely missed many, please drop me a message if you want to be added
go.bsky.app/5Gzo7oZ
go.bsky.app/5Gzo7oZ
November 11, 2024 at 1:09 PM
I started a list of people working broadly on cell adhesion and cell migration. I likely missed many, please drop me a message if you want to be added
go.bsky.app/5Gzo7oZ
go.bsky.app/5Gzo7oZ
Reposted by Juanma García-Arcos
I have created a starter pack of scientists in der Schweiz so we can all hopefully find each other 🇨🇭🧪
go.bsky.app/8rF765Z
go.bsky.app/8rF765Z
November 19, 2024 at 8:46 PM
I have created a starter pack of scientists in der Schweiz so we can all hopefully find each other 🇨🇭🧪
go.bsky.app/8rF765Z
go.bsky.app/8rF765Z
Reposted by Juanma García-Arcos
This is a movie of actin filaments in a cultured cell. I like watching it and I hope you do to.
November 18, 2024 at 5:50 AM
This is a movie of actin filaments in a cultured cell. I like watching it and I hope you do to.