Juanma García-Arcos
@juanmagararc.bsky.social
Group leader at EPFL, Lausanne. Formerly, Postdoc Roux lab, Geneva; PhD Piel lab, Paris. #cytoplasts #actomyosin #cellbio #microscopy #membranes #biophysics #extracellular_vesicles #sciart
Lab website: http://celldynamicslab.com
Lab website: http://celldynamicslab.com
Reposted by Juanma García-Arcos
Felicidades querido JuanMa !
El Breaking lab, o el lab FLIPado (de FLIPR) será enorme...
El Breaking lab, o el lab FLIPado (de FLIPR) será enorme...
elmo from sesame street is surrounded by flames and smoke
ALT: elmo from sesame street is surrounded by flames and smoke
media.tenor.com
November 15, 2025 at 6:50 PM
Felicidades querido JuanMa !
El Breaking lab, o el lab FLIPado (de FLIPR) será enorme...
El Breaking lab, o el lab FLIPado (de FLIPR) será enorme...
*ALL*source data and Python notebooks are available (you can reproduce all figures!)
🔗 doi.org/10.6084/m9.f...
We hope this helps make lifetime analysis transparent and comparable across setups.
📄 www.biorxiv.org/content/10.1...
@rouxlab.bsky.social
#FLIM #FlipperTR #Microscopy #OpenScience
🔗 doi.org/10.6084/m9.f...
We hope this helps make lifetime analysis transparent and comparable across setups.
📄 www.biorxiv.org/content/10.1...
@rouxlab.bsky.social
#FLIM #FlipperTR #Microscopy #OpenScience
Data and codes
Data and codes corresponding to the preprint:
doi.org
October 5, 2025 at 4:04 PM
*ALL*source data and Python notebooks are available (you can reproduce all figures!)
🔗 doi.org/10.6084/m9.f...
We hope this helps make lifetime analysis transparent and comparable across setups.
📄 www.biorxiv.org/content/10.1...
@rouxlab.bsky.social
#FLIM #FlipperTR #Microscopy #OpenScience
🔗 doi.org/10.6084/m9.f...
We hope this helps make lifetime analysis transparent and comparable across setups.
📄 www.biorxiv.org/content/10.1...
@rouxlab.bsky.social
#FLIM #FlipperTR #Microscopy #OpenScience
Even at realistic photon-limited conditions (~5–10 photons/pixel):
• Phasor analysis remains robust and resilient to noise, although harder to quantify in commercial software.
• Fast FLIM gives clean kinetics but depends on setup
• Fitting yields the most amount of information in ideal situations
• Phasor analysis remains robust and resilient to noise, although harder to quantify in commercial software.
• Fast FLIM gives clean kinetics but depends on setup
• Fitting yields the most amount of information in ideal situations
October 5, 2025 at 4:04 PM
Even at realistic photon-limited conditions (~5–10 photons/pixel):
• Phasor analysis remains robust and resilient to noise, although harder to quantify in commercial software.
• Fast FLIM gives clean kinetics but depends on setup
• Fitting yields the most amount of information in ideal situations
• Phasor analysis remains robust and resilient to noise, although harder to quantify in commercial software.
• Fast FLIM gives clean kinetics but depends on setup
• Fitting yields the most amount of information in ideal situations
We also compared Fast FLIM, Fitting and Phasor estimators during osmotic shocks, a direct test of membrane tension.
All track the expected lifetime shifts, but with distinct sensitivities and noise behaviors.
All track the expected lifetime shifts, but with distinct sensitivities and noise behaviors.
October 5, 2025 at 4:04 PM
We also compared Fast FLIM, Fitting and Phasor estimators during osmotic shocks, a direct test of membrane tension.
All track the expected lifetime shifts, but with distinct sensitivities and noise behaviors.
All track the expected lifetime shifts, but with distinct sensitivities and noise behaviors.
We quantified the shot-noise constant (c), the factor translating photon number (N) to lifetime standard deviation (σ): σ ∝ c N⁻⁰·⁵.
📊 Shot-noise constant results: Leica yields consistent photon efficiency; PicoQuant varies by estimator.
📊 Shot-noise constant results: Leica yields consistent photon efficiency; PicoQuant varies by estimator.
October 5, 2025 at 4:04 PM
We quantified the shot-noise constant (c), the factor translating photon number (N) to lifetime standard deviation (σ): σ ∝ c N⁻⁰·⁵.
📊 Shot-noise constant results: Leica yields consistent photon efficiency; PicoQuant varies by estimator.
📊 Shot-noise constant results: Leica yields consistent photon efficiency; PicoQuant varies by estimator.
We show the behavior of tau average and tau 1 across a range of packing standards (GUV with known lipid compositions), showing that tau average has less variability than tau 1.
October 5, 2025 at 4:04 PM
We show the behavior of tau average and tau 1 across a range of packing standards (GUV with known lipid compositions), showing that tau average has less variability than tau 1.
Flipper-TR is now a standard probe for lipid packing and membrane tension, but everyone analyses it differently.
We compared:
• Fast FLIM (mean photon arrival)
• Bi-exponential reconvolution & tail fits
• Phasor analysis
…across photon counts, repetition rates (20–80 MHz), and different set-ups.
We compared:
• Fast FLIM (mean photon arrival)
• Bi-exponential reconvolution & tail fits
• Phasor analysis
…across photon counts, repetition rates (20–80 MHz), and different set-ups.
October 5, 2025 at 4:04 PM
Flipper-TR is now a standard probe for lipid packing and membrane tension, but everyone analyses it differently.
We compared:
• Fast FLIM (mean photon arrival)
• Bi-exponential reconvolution & tail fits
• Phasor analysis
…across photon counts, repetition rates (20–80 MHz), and different set-ups.
We compared:
• Fast FLIM (mean photon arrival)
• Bi-exponential reconvolution & tail fits
• Phasor analysis
…across photon counts, repetition rates (20–80 MHz), and different set-ups.
We first review what we know about the photophysics of the Flipper-TR probe and how this lipid packing probe can be used as a tension reporter
October 5, 2025 at 4:04 PM
We first review what we know about the photophysics of the Flipper-TR probe and how this lipid packing probe can be used as a tension reporter