Here, the authors discuss principles of experimental design that are relevant for all biology research, along with special considerations for projects using -omics approaches, highlighting common expe...

Mass spectrometry plays a crucial role for qualitative and quantitative analysis in various scientific fields. For LC-MS, ESI is the most frequently used ionization technique. However, ionization processes are still not fully understood, and ion enhancement or ion suppression effects can usually be found during ESI. In the current work, adduct formation by coeluting (endogenous) compounds was observed for the diuretic hydrochlorothiazide during negative mode ionization, decreasing the corresponding deprotonated hydrochlorothiazide signal. After identification of the corresponding adduct partners, being hippuric acid and indoxyl sulfate, systematic investigations on this formation were conducted using different concentrations of both compounds. Additionally, APCI and different ESI sources, as well as different mobile phase compositions, were tested. It was shown that the adduct formation was only found by ESI and was independent of vendor-specific source design. The adduct formation was also independent of the composition of the mobile phases and was found to be dependent on the concentration of the coeluting compounds. An over-additive effect on hydrochlorothiazide signal loss was found if hippuric acid and indoxyl sulfate adducts were formed. Finally, adduct formation was studied for other structural related and non-structural related compounds. For compounds with a high(er) structural similarity to hydrochlorothiazide (namely: bendroflumethiazide, buthiazide, cyclopenthiazide, and dorzolamide), adduct formation was observed. This study provided new insights into the ESI process. Graphical Abstract

Lipid dysregulation is increasingly recognized as a key feature of Parkinson′s disease (PD). A central unresolved question, however, is whether lipidomic signatures identified in accessible peripheral...

Many pressing medical challenges, such as diagnosing disease, enhancing directed stem-cell differentiation, and classifying cancers, have long been hindered by limitations in our ability to quantify p...

Cells tightly control the homeostatic levels and subcellular localizations of membrane phospholipids through the regulation of the activities of numerous lipid-metabolizing enzymes and lipid transfer ...

This study reveals how cardiolipin governs mitochondrial morphology by modulating the activity of human OPA1 and how its replacement by monolyso-cardiolipin, as observed in Barth syndrome, impacts mitochondrial membrane-shaping mechanisms.

The cell membrane facilitates interactions with the environment and serves as an organizational platform for coordinating cellular processes, with lipids playing a central role in determining membrane...

Meyer and colleagues refute the idea that, as aging can be tracked precisely by clocks, it must be driven by a biological program. They propose that imperfect maintenance and repair processes resultin...

An approach combining bioorthogonal chemistry with genetically encoded fluorogen-activating proteins enables subcellular imaging of phospholipids and glycans, as well as the visualization of lipid tra...

Membranes are protein and lipid structures that surround cells and other biological compartments. We present a conceptual model wherein all membranes are organized into structural and functional zones...

MSnLib is a large-scale, open MSn spectral library featuring >2.3 million MSn and >357,000 MS2 spectra for 30,008 unique small molecules.

Over the past decade the use of electrospray ionization mass spectrometry (ESI-MS) has been extended from the domain of chemical analysis to that of chemical synthesis. This development was spurred by...

We, researchers, students, and members of the administrative and technical staff of the University of Lausanne (UNIL), firmly oppose the budget cuts announced for UNIL by the government of Vaud on 24t...

These findings suggest that wearable sensors provide a non-invasive method to monitor pregnancy-related physiological and behavioural changes, which align with hormonal shifts. This study provides population-level insights in live birth pregnancies, and an exploratory analysis of adverse outcomes reflecting the feasibility of recruiting and capturing physiological signals also in these cases. This approach may enable early risk assessment for adverse pregnancy outcomes, including miscarriage and preterm birth.