Dimitrios L. Wagner
@dlwagner.bsky.social
Physician & science enthusiast @BCMHouston @ChariteBerlin @BIHregeneration // Genome Editing and Immunology // 50% of @immunoboys.bsky.social / @dlwagnerlabs.bsky.social
Pinned
Dimitrios L. Wagner
@dlwagner.bsky.social
· Sep 19
Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases
Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. H...
www.biorxiv.org
You like engineering T cells, but you are missing the right flavor?
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Reposted by Dimitrios L. Wagner
November 24, 2025 at 11:46 PM
Reposted by Dimitrios L. Wagner
Adapting the 10x workflow to detect EBV reads, Younis et al show that EBV-transcribing B cells are increased in SLE patients, especially among CD27+CD21low B cells. They have autoreactive BCRs and are more poised for antigen-presentation.
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
November 13, 2025 at 10:48 PM
Adapting the 10x workflow to detect EBV reads, Younis et al show that EBV-transcribing B cells are increased in SLE patients, especially among CD27+CD21low B cells. They have autoreactive BCRs and are more poised for antigen-presentation.
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
Reposted by Dimitrios L. Wagner
What mechanisms drive checkpoint inhibitor-associated interstitial nephritis (ICI-AIN)?
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
November 5, 2025 at 6:24 PM
What mechanisms drive checkpoint inhibitor-associated interstitial nephritis (ICI-AIN)?
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
Reposted by Dimitrios L. Wagner
A monoclonal antibody that inhibits the shedding of CD16a and CD16b and promotes antibody-dependent cellular cytotoxicity against tumors @natcomms.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 12, 2025 at 12:56 AM
A monoclonal antibody that inhibits the shedding of CD16a and CD16b and promotes antibody-dependent cellular cytotoxicity against tumors @natcomms.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Dimitrios L. Wagner
(1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.
November 4, 2025 at 5:58 PM
(1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.
Reposted by Dimitrios L. Wagner
Ubiquitinylation is more complex than just a signal for proteasomal degradation: Linear ubiquitinylation of Methionine-1 is important for NFkB signalling.
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
October 20, 2025 at 7:51 PM
Ubiquitinylation is more complex than just a signal for proteasomal degradation: Linear ubiquitinylation of Methionine-1 is important for NFkB signalling.
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
Reposted by Dimitrios L. Wagner
Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
Cell-stereotyped DNA repair outcomes are widespread during genome editing
Genome editing outcomes are governed by DNA repair pathways that vary with cell type and state. We developed scOUT-seq (single-cell Outcomes Using Transcript sequencing), a scalable approach that join...
www.biorxiv.org
October 24, 2025 at 8:44 AM
Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
Reposted by Dimitrios L. Wagner
A new subspecialty takes off! Astroimmunology is focused on the effects of #spaceflight on the #immunesystem and defines avenues for countermeasures. A bonus: the work will also inform research on #aging @natrevimmunol.nature.com evimmunol.nature.com www.buckinstitute.org/news/a-hitch...
A hitchhiker’s guide to the galaxy of space immunology
Buck scientists help define the scope of Astroimmunology With the advent of commercial spaceflight, an increasing number of people may be heading into space in the coming years. Some will even get ...
www.buckinstitute.org
October 16, 2025 at 4:20 PM
A new subspecialty takes off! Astroimmunology is focused on the effects of #spaceflight on the #immunesystem and defines avenues for countermeasures. A bonus: the work will also inform research on #aging @natrevimmunol.nature.com evimmunol.nature.com www.buckinstitute.org/news/a-hitch...
Reposted by Dimitrios L. Wagner
BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)
October 15, 2025 at 12:22 PM
BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)
Reposted by Dimitrios L. Wagner
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Enhancer-promoter compatibility is mediated by the promoter-proximal region
Gene promoters induce transcription in response to distal enhancers. How enhancers specifically activate their target promoter while bypassing other promoters remains unclear. Here, we find that the p...
www.biorxiv.org
October 16, 2025 at 3:06 PM
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Reposted by Dimitrios L. Wagner
Honored to be part of the 2025 #STATWunderkinds! Grateful for the many supportive mentors, colleagues, and friends with special shoutout to @bkleinstiver.bsky.social.
Full list: www.statnews.com/wunderkinds/
@statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social
Full list: www.statnews.com/wunderkinds/
@statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social
October 15, 2025 at 11:55 AM
Honored to be part of the 2025 #STATWunderkinds! Grateful for the many supportive mentors, colleagues, and friends with special shoutout to @bkleinstiver.bsky.social.
Full list: www.statnews.com/wunderkinds/
@statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social
Full list: www.statnews.com/wunderkinds/
@statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social
“ ‘Off the shelf’ Treg may soon be… on the shelf. “ - awesome quote by Prof. Fadi Issa.
Check out our new story: 👇
www.nature.com/articles/s41...
One week after the Nobel prize for Treg 🍀
Check out our new story: 👇
www.nature.com/articles/s41...
One week after the Nobel prize for Treg 🍀
October 13, 2025 at 11:09 PM
“ ‘Off the shelf’ Treg may soon be… on the shelf. “ - awesome quote by Prof. Fadi Issa.
Check out our new story: 👇
www.nature.com/articles/s41...
One week after the Nobel prize for Treg 🍀
Check out our new story: 👇
www.nature.com/articles/s41...
One week after the Nobel prize for Treg 🍀
“Good things take time”
Allogeneic Treg do not prevent their own rejection in immunocompetent hosts - HLA matching or HLA editing required for optional function!
A beautiful collaboration with Fadi Issa & Joanna Hester’s team @ox.ac.uk. @dlwagnerlabs.bsky.social
Allogeneic Treg do not prevent their own rejection in immunocompetent hosts - HLA matching or HLA editing required for optional function!
A beautiful collaboration with Fadi Issa & Joanna Hester’s team @ox.ac.uk. @dlwagnerlabs.bsky.social
HLA matching or CRISPR editing HLA-I/II enables engraftment & function of allo human Treg therapy in a humanized mouse transplantation model @natcomms.nature.com @dlwagner.bsky.social @bcmhouston.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
October 13, 2025 at 9:30 PM
“Good things take time”
Allogeneic Treg do not prevent their own rejection in immunocompetent hosts - HLA matching or HLA editing required for optional function!
A beautiful collaboration with Fadi Issa & Joanna Hester’s team @ox.ac.uk. @dlwagnerlabs.bsky.social
Allogeneic Treg do not prevent their own rejection in immunocompetent hosts - HLA matching or HLA editing required for optional function!
A beautiful collaboration with Fadi Issa & Joanna Hester’s team @ox.ac.uk. @dlwagnerlabs.bsky.social
Reposted by Dimitrios L. Wagner
HLA matching or CRISPR editing HLA-I/II enables engraftment & function of allo human Treg therapy in a humanized mouse transplantation model @natcomms.nature.com @dlwagner.bsky.social @bcmhouston.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
October 13, 2025 at 5:39 PM
HLA matching or CRISPR editing HLA-I/II enables engraftment & function of allo human Treg therapy in a humanized mouse transplantation model @natcomms.nature.com @dlwagner.bsky.social @bcmhouston.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
@dlwagnerlabs.bsky.social on tour! 🌍🇩🇪🇺🇸
We had a great time at the Annual Congress of the European Society for Gene and Cell Therapy #ESGCT2025 in Sevilla. 🇪🇸
Super proud of everyone who presented their projects & grateful for working with this amazing team: science and good vibes! 🧬🧪💙
We had a great time at the Annual Congress of the European Society for Gene and Cell Therapy #ESGCT2025 in Sevilla. 🇪🇸
Super proud of everyone who presented their projects & grateful for working with this amazing team: science and good vibes! 🧬🧪💙
October 12, 2025 at 1:45 PM
@dlwagnerlabs.bsky.social on tour! 🌍🇩🇪🇺🇸
We had a great time at the Annual Congress of the European Society for Gene and Cell Therapy #ESGCT2025 in Sevilla. 🇪🇸
Super proud of everyone who presented their projects & grateful for working with this amazing team: science and good vibes! 🧬🧪💙
We had a great time at the Annual Congress of the European Society for Gene and Cell Therapy #ESGCT2025 in Sevilla. 🇪🇸
Super proud of everyone who presented their projects & grateful for working with this amazing team: science and good vibes! 🧬🧪💙
Reposted by Dimitrios L. Wagner
October 11, 2025 at 11:02 AM
Reposted by Dimitrios L. Wagner
TCR activation impairs CAR-T cytotoxicity against separate target cells https://www.biorxiv.org/content/10.1101/2025.10.07.680634v1
October 8, 2025 at 12:15 AM
TCR activation impairs CAR-T cytotoxicity against separate target cells https://www.biorxiv.org/content/10.1101/2025.10.07.680634v1
Reposted by Dimitrios L. Wagner
Our team is on their way to the ESGCT conference 2025! Excited for this upcoming week full of science, talks, and inspiring discussions with the cell & gene therapy community!
Starting with the HDR workshop - talks by Isabell (‘one-pot’ PASTA) & Viktor
(BEKI) + posters by Ana, Lily, Jonas and Laura
Starting with the HDR workshop - talks by Isabell (‘one-pot’ PASTA) & Viktor
(BEKI) + posters by Ana, Lily, Jonas and Laura
October 5, 2025 at 8:51 PM
Our team is on their way to the ESGCT conference 2025! Excited for this upcoming week full of science, talks, and inspiring discussions with the cell & gene therapy community!
Starting with the HDR workshop - talks by Isabell (‘one-pot’ PASTA) & Viktor
(BEKI) + posters by Ana, Lily, Jonas and Laura
Starting with the HDR workshop - talks by Isabell (‘one-pot’ PASTA) & Viktor
(BEKI) + posters by Ana, Lily, Jonas and Laura
Reposted by Dimitrios L. Wagner
Excited to share our preprint📯:
We introduce #IntegrateALL, an open-source RNA-seq pipeline for interpretable molecular diagnostics in acute lymphoblastic leukemia!🧬
Big thanks to Nadine Wolgast & team @ www.uksh.de
, catchall-kfo5010.com!
See: doi.org/p7k5 and 🧵
We introduce #IntegrateALL, an open-source RNA-seq pipeline for interpretable molecular diagnostics in acute lymphoblastic leukemia!🧬
Big thanks to Nadine Wolgast & team @ www.uksh.de
, catchall-kfo5010.com!
See: doi.org/p7k5 and 🧵
September 28, 2025 at 8:24 AM
Excited to share our preprint📯:
We introduce #IntegrateALL, an open-source RNA-seq pipeline for interpretable molecular diagnostics in acute lymphoblastic leukemia!🧬
Big thanks to Nadine Wolgast & team @ www.uksh.de
, catchall-kfo5010.com!
See: doi.org/p7k5 and 🧵
We introduce #IntegrateALL, an open-source RNA-seq pipeline for interpretable molecular diagnostics in acute lymphoblastic leukemia!🧬
Big thanks to Nadine Wolgast & team @ www.uksh.de
, catchall-kfo5010.com!
See: doi.org/p7k5 and 🧵
Reposted by Dimitrios L. Wagner
Big shoutout to the whole group who made #IntegrateALL possible - especially @alinamh.bsky.social, Thomas Beder and Claudia Baldus at www.catchall-kfo5010.com
September 28, 2025 at 8:36 AM
Big shoutout to the whole group who made #IntegrateALL possible - especially @alinamh.bsky.social, Thomas Beder and Claudia Baldus at www.catchall-kfo5010.com
Reposted by Dimitrios L. Wagner
Potentiating CAR-T bystander killing by enhanced Fas/FasL signaling mitigates antigen escape in heterogeneous tumors https://www.biorxiv.org/content/10.1101/2025.09.22.677496v1
September 25, 2025 at 2:16 AM
Potentiating CAR-T bystander killing by enhanced Fas/FasL signaling mitigates antigen escape in heterogeneous tumors https://www.biorxiv.org/content/10.1101/2025.09.22.677496v1
Reposted by Dimitrios L. Wagner
Check out one of our latest stories about combining our non viral knock-in strategy with integrases for an efficient, targeted integration of transgenes >8kb in human T cells!
‘One-pot’ PASTA: programmable and site specific transgene addition - all in just one reaction 🫕
‘One-pot’ PASTA: programmable and site specific transgene addition - all in just one reaction 🫕
September 23, 2025 at 4:58 AM
Check out one of our latest stories about combining our non viral knock-in strategy with integrases for an efficient, targeted integration of transgenes >8kb in human T cells!
‘One-pot’ PASTA: programmable and site specific transgene addition - all in just one reaction 🫕
‘One-pot’ PASTA: programmable and site specific transgene addition - all in just one reaction 🫕
Reposted by Dimitrios L. Wagner
This has been such a great collaboration with @dlwagnerlabs.bsky.social! You really need to check it out
One-pot PASTA 🍝 - a one-step protocol to knock-in large transgenes.
I feel tempted to think about one-pot PIZZA 🍕 next!
First authors Isabell and @therealjonaskath.bsky.social were amazing!!
One-pot PASTA 🍝 - a one-step protocol to knock-in large transgenes.
I feel tempted to think about one-pot PIZZA 🍕 next!
First authors Isabell and @therealjonaskath.bsky.social were amazing!!
You like engineering T cells, but you are missing the right flavor?
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases
Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. H...
www.biorxiv.org
September 19, 2025 at 3:37 PM
This has been such a great collaboration with @dlwagnerlabs.bsky.social! You really need to check it out
One-pot PASTA 🍝 - a one-step protocol to knock-in large transgenes.
I feel tempted to think about one-pot PIZZA 🍕 next!
First authors Isabell and @therealjonaskath.bsky.social were amazing!!
One-pot PASTA 🍝 - a one-step protocol to knock-in large transgenes.
I feel tempted to think about one-pot PIZZA 🍕 next!
First authors Isabell and @therealjonaskath.bsky.social were amazing!!
Reposted by Dimitrios L. Wagner
Super-large targeted Knock-Ins in primary T cells, with multigene inserts >8kb, in a single reaction. HOW?? 🙀
Combine simple CRISPR-HDR & Integrases for one-pot PASTA🍝
= Programmable And Site-specific Transgene Addition. 😎
Combine simple CRISPR-HDR & Integrases for one-pot PASTA🍝
= Programmable And Site-specific Transgene Addition. 😎
September 19, 2025 at 1:33 PM
Super-large targeted Knock-Ins in primary T cells, with multigene inserts >8kb, in a single reaction. HOW?? 🙀
Combine simple CRISPR-HDR & Integrases for one-pot PASTA🍝
= Programmable And Site-specific Transgene Addition. 😎
Combine simple CRISPR-HDR & Integrases for one-pot PASTA🍝
= Programmable And Site-specific Transgene Addition. 😎
You like engineering T cells, but you are missing the right flavor?
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases
Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. H...
www.biorxiv.org
September 19, 2025 at 5:30 AM
You like engineering T cells, but you are missing the right flavor?
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪