Lennard Ostendorf
@lostendorf.bsky.social
MD/PhD – Postdoc Brigham/Harvard Med
Immunology. Kidneys. Autoimmune Disorders. Basketball. Jazz.
50% of the @immunoboys.bsky.social
Immunology. Kidneys. Autoimmune Disorders. Basketball. Jazz.
50% of the @immunoboys.bsky.social
Our case report of a patient developing drug-induced systemic sclerosis after bleomycin exposure – still showing antibodies after 5 years. Nicely done Philipp 🎉 #rheumsky
authors.elsevier.com/sd/article/S...
authors.elsevier.com/sd/article/S...
December 6, 2025 at 10:47 PM
Our case report of a patient developing drug-induced systemic sclerosis after bleomycin exposure – still showing antibodies after 5 years. Nicely done Philipp 🎉 #rheumsky
authors.elsevier.com/sd/article/S...
authors.elsevier.com/sd/article/S...
Reposted by Lennard Ostendorf
Join us at Charité University and the IMPRS-IDI Graduate Program in Berlin to unravel NK cell clonality and memory! We are looking for motivated PhD candidates who are passionate about immunology. @romagnanilab.bsky.social
@timorckert.bsky.social
Apply now 👉 www.imprs-idi.org/home/
@timorckert.bsky.social
Apply now 👉 www.imprs-idi.org/home/
November 14, 2025 at 2:39 PM
Join us at Charité University and the IMPRS-IDI Graduate Program in Berlin to unravel NK cell clonality and memory! We are looking for motivated PhD candidates who are passionate about immunology. @romagnanilab.bsky.social
@timorckert.bsky.social
Apply now 👉 www.imprs-idi.org/home/
@timorckert.bsky.social
Apply now 👉 www.imprs-idi.org/home/
Reposted by Lennard Ostendorf
Our paper on the second Berlin patient came out in Nature today and I’m really happy we can share this story on World AIDS Day. I want to thank everyone on the team and all co-authors for their work over the years.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Sustained HIV-1 remission after heterozygous CCR5Δ32 stem cell transplantation - Nature
Nature - Sustained HIV-1 remission after heterozygous CCR5Δ32 stem cell transplantation
www.nature.com
December 1, 2025 at 10:40 PM
Our paper on the second Berlin patient came out in Nature today and I’m really happy we can share this story on World AIDS Day. I want to thank everyone on the team and all co-authors for their work over the years.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Lennard Ostendorf
Reposted by Lennard Ostendorf
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
#protistsonsky 🧵
November 25, 2025 at 8:41 PM
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
#protistsonsky 🧵
Reposted by Lennard Ostendorf
Reposted by Lennard Ostendorf
Just 4 days until the start of I2K and its 33 totally free image analysis tutorials and events! Please share with your "home networks", especially early career researchers - the videos will be amazing and high-impact no matter how many people attend live, BUT (1/x)
November 13, 2025 at 2:32 PM
Just 4 days until the start of I2K and its 33 totally free image analysis tutorials and events! Please share with your "home networks", especially early career researchers - the videos will be amazing and high-impact no matter how many people attend live, BUT (1/x)
Reposted by Lennard Ostendorf
Adapting the 10x workflow to detect EBV reads, Younis et al show that EBV-transcribing B cells are increased in SLE patients, especially among CD27+CD21low B cells. They have autoreactive BCRs and are more poised for antigen-presentation.
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
November 13, 2025 at 10:48 PM
Adapting the 10x workflow to detect EBV reads, Younis et al show that EBV-transcribing B cells are increased in SLE patients, especially among CD27+CD21low B cells. They have autoreactive BCRs and are more poised for antigen-presentation.
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
What mechanisms drive checkpoint inhibitor-associated interstitial nephritis (ICI-AIN)?
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
November 5, 2025 at 6:24 PM
What mechanisms drive checkpoint inhibitor-associated interstitial nephritis (ICI-AIN)?
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
Reposted by Lennard Ostendorf
science is a TEAM SPORT
there is no ISOLEUCINE in THREONINE GLUTAMATE ALANINE METHIONINE
there is no ISOLEUCINE in THREONINE GLUTAMATE ALANINE METHIONINE
October 31, 2025 at 6:58 PM
science is a TEAM SPORT
there is no ISOLEUCINE in THREONINE GLUTAMATE ALANINE METHIONINE
there is no ISOLEUCINE in THREONINE GLUTAMATE ALANINE METHIONINE
Thylacine – very cool multi-instrumental electronic session #musicsky
www.youtube.com/watch?v=NM51...
www.youtube.com/watch?v=NM51...
THYLACINE - Live session 2024 @ Intuitive Studio
YouTube video by Thylacine
www.youtube.com
October 23, 2025 at 1:14 PM
Thylacine – very cool multi-instrumental electronic session #musicsky
www.youtube.com/watch?v=NM51...
www.youtube.com/watch?v=NM51...
Reposted by Lennard Ostendorf
🧠🌟🐭 Excited to share some of my postdoc work on the evolution of dexterity!
We compared deer mice evolved in forest vs prairie habitats. We found that forest mice have:
(1) more corticospinal neurons (CSNs)
(2) better hand dexterity
(3) more dexterous climbing, which is linked to CSN number🧵
We compared deer mice evolved in forest vs prairie habitats. We found that forest mice have:
(1) more corticospinal neurons (CSNs)
(2) better hand dexterity
(3) more dexterous climbing, which is linked to CSN number🧵
October 22, 2025 at 8:41 PM
🧠🌟🐭 Excited to share some of my postdoc work on the evolution of dexterity!
We compared deer mice evolved in forest vs prairie habitats. We found that forest mice have:
(1) more corticospinal neurons (CSNs)
(2) better hand dexterity
(3) more dexterous climbing, which is linked to CSN number🧵
We compared deer mice evolved in forest vs prairie habitats. We found that forest mice have:
(1) more corticospinal neurons (CSNs)
(2) better hand dexterity
(3) more dexterous climbing, which is linked to CSN number🧵
Reposted by Lennard Ostendorf
Ubiquitinylation is more complex than just a signal for proteasomal degradation: Linear ubiquitinylation of Methionine-1 is important for NFkB signalling.
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
October 20, 2025 at 7:51 PM
Ubiquitinylation is more complex than just a signal for proteasomal degradation: Linear ubiquitinylation of Methionine-1 is important for NFkB signalling.
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
Reposted by Lennard Ostendorf
Tregs in the skin reduce the activation of sensory neurons by producing enkephalin. This inhibits secondary inflammatory changes mediated by the neurons. (in 🐭)
🧪 ⑂
Mendoza et al 2025 in Science Immunology
www.science.org/doi/full/10....
🧪 ⑂
Mendoza et al 2025 in Science Immunology
www.science.org/doi/full/10....
October 18, 2025 at 12:17 AM
Tregs in the skin reduce the activation of sensory neurons by producing enkephalin. This inhibits secondary inflammatory changes mediated by the neurons. (in 🐭)
🧪 ⑂
Mendoza et al 2025 in Science Immunology
www.science.org/doi/full/10....
🧪 ⑂
Mendoza et al 2025 in Science Immunology
www.science.org/doi/full/10....
Reposted by Lennard Ostendorf
HLA matching or CRISPR editing HLA-I/II enables engraftment & function of allo human Treg therapy in a humanized mouse transplantation model @natcomms.nature.com @dlwagner.bsky.social @bcmhouston.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
October 13, 2025 at 5:39 PM
HLA matching or CRISPR editing HLA-I/II enables engraftment & function of allo human Treg therapy in a humanized mouse transplantation model @natcomms.nature.com @dlwagner.bsky.social @bcmhouston.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Lennard Ostendorf
CRISPR screening factors influencing Th1 vs Th2 differentiation, RAMP3 which forms part of a CGRP receptor is a positive regulator of Th1 differention. CGRP acts by increasing intracellular cAMP and ultimately ATF3 activity it induces STAT1 and thereby Th1 differentiation. in 🐭
🧪 ⑂ 1/2
🧪 ⑂ 1/2
October 13, 2025 at 12:27 AM
CRISPR screening factors influencing Th1 vs Th2 differentiation, RAMP3 which forms part of a CGRP receptor is a positive regulator of Th1 differention. CGRP acts by increasing intracellular cAMP and ultimately ATF3 activity it induces STAT1 and thereby Th1 differentiation. in 🐭
🧪 ⑂ 1/2
🧪 ⑂ 1/2
Reposted by Lennard Ostendorf
Friday Papers Club! 🍿In light of Pubmed going down, the collapse of social media, and my recent success at getting recommendations for interesting papers from those still around, I am starting a new tradition! - Recommend a good scientific manuscript! 👇
October 3, 2025 at 9:10 AM
Friday Papers Club! 🍿In light of Pubmed going down, the collapse of social media, and my recent success at getting recommendations for interesting papers from those still around, I am starting a new tradition! - Recommend a good scientific manuscript! 👇
Reposted by Lennard Ostendorf
You like engineering T cells, but you are missing the right flavor?
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases
Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. H...
www.biorxiv.org
September 19, 2025 at 5:30 AM
You like engineering T cells, but you are missing the right flavor?
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Reposted by Lennard Ostendorf
A conference of three primates, discussing videography and their varying perspectives on nature documentaries.
Jane Goodall and husband Hugo van Lawick, 1974.
Third primate's name unknown.
Jane Goodall and husband Hugo van Lawick, 1974.
Third primate's name unknown.
August 30, 2024 at 11:30 AM
A conference of three primates, discussing videography and their varying perspectives on nature documentaries.
Jane Goodall and husband Hugo van Lawick, 1974.
Third primate's name unknown.
Jane Goodall and husband Hugo van Lawick, 1974.
Third primate's name unknown.
Reposted by Lennard Ostendorf
Jane Goodall, the trailblazing naturalist whose intimate observations of chimpanzees in the African wild produced powerful insights that transformed basic conceptions of humankind, has died. She was 91.
Read more about her legacy www.latimes.com/obituaries/s...
Read more about her legacy www.latimes.com/obituaries/s...
October 1, 2025 at 6:11 PM
Jane Goodall, the trailblazing naturalist whose intimate observations of chimpanzees in the African wild produced powerful insights that transformed basic conceptions of humankind, has died. She was 91.
Read more about her legacy www.latimes.com/obituaries/s...
Read more about her legacy www.latimes.com/obituaries/s...
Reposted by Lennard Ostendorf
For this #FluorescenceFriday, a gorgeous image of an adult mouse kidney labeled with AQP2 and alpha SMA antibodies. AQP2 (green) marks the collecting duct and distal connecting segment while SMA marks the arterial tree. Courtesy of talented postdoc Sarah McLarnon.
September 26, 2025 at 5:51 PM
For this #FluorescenceFriday, a gorgeous image of an adult mouse kidney labeled with AQP2 and alpha SMA antibodies. AQP2 (green) marks the collecting duct and distal connecting segment while SMA marks the arterial tree. Courtesy of talented postdoc Sarah McLarnon.
Rarely is a taped live performance so much better than the original. Love this Maribou State recording start to finish 🔥
Great for doing annoying point and click data analysis today.
www.youtube.com/watch?v=oQjg2QEvEX0
Great for doing annoying point and click data analysis today.
www.youtube.com/watch?v=oQjg2QEvEX0
Maribou State Live From Alexandra Palace, London
YouTube video by DJ Mag
www.youtube.com
September 26, 2025 at 4:30 PM
Rarely is a taped live performance so much better than the original. Love this Maribou State recording start to finish 🔥
Great for doing annoying point and click data analysis today.
www.youtube.com/watch?v=oQjg2QEvEX0
Great for doing annoying point and click data analysis today.
www.youtube.com/watch?v=oQjg2QEvEX0
Reposted by Lennard Ostendorf
For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
currentprotocols.onlinelibrary.wiley.com
September 19, 2025 at 2:39 PM
For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...