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Reposted by Luis Bonet-Ponce

Christophe 🔬 L @christlet.bsky.social · 4h

Interesting question. For me as a visually-driven person, consistency and formatting qualities have a direct effect on my understanding. I see high quality as an inclusion strategy - bad presentation will keep some people from getting it

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Reposted by Luis Bonet-Ponce

Manuel Thery @manuelthery.bsky.social · 1d

We hire!
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...

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Luis Bonet-Ponce @lysoluis.bsky.social · 1d

Really cool work, congrats Heidi!

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Luis Bonet-Ponce @lysoluis.bsky.social · 1d

🚨 We’re hiring a postdoc! 🚨
Interested in lysosomes, Parkinson’s disease, cell biology, microscopy — or all of the above? Come work with us at the Bonet-Ponce Lab!
Reach out directly: [email protected]
Learn more: bonetponcelab.com
Let’s chat!

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Reposted by Luis Bonet-Ponce

Heidi M McBride @mitodynamics.bsky.social · 1d

New paper - MAPL strikes again! Interested in mitochondrial signalling, inflammation, lysosome biology, pyroptosis, and Parkinson's disease? Have a look, there's something for everyone! Feeling grateful! @mitocollier.bsky.social Funded by #CIHR, @asapresearch.parkinsonsroadmap.org.
rdcu.be/eKKz1 🇨🇦

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Reposted by Luis Bonet-Ponce

Kevin Terretaz @kwolbachia.bsky.social · 11d

I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager!
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...

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Reposted by Luis Bonet-Ponce

Victor Tatarskiy @greenrat.bsky.social · 13d

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky

On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.

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Reposted by Luis Bonet-Ponce

Christophe 🔬 L @christlet.bsky.social · 12d

Nanotubes everywhere between neurons! A bit skeptical about these "dendrite-dendrite nanotubes" from a phalloidin staining in culture, but let's read on… www.science.org/doi/10.1126/...

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Reposted by Luis Bonet-Ponce

De Camilli Lab @pdc-lab.bsky.social · 16d

"Impaired hematopoiesis and embryonic lethality at midgestation of mice lacking both lipid transfer proteins VPS13A and VPS13C" out now in PLOS Biology
journals.plos.org/plosbiology/...

Impaired hematopoiesis and embryonic lethality at midgestation of mice lacking both lipid transfer proteins VPS13A and VPS13C

VPS13A and VPS13C are bridge-like lipid transport proteins with distinct subcellular localization and function, and their absence is linked with chorea-acanthocytosis and Parkinson's disease, respecti...

journals.plos.org

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Luis Bonet-Ponce @lysoluis.bsky.social · 19d

Huge compliment coming from you, tempted to put it on my CV 🙂!

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Reposted by Luis Bonet-Ponce

Christophe 🔬 L @christlet.bsky.social · 19d

Beautiful live-cell imaging of lysosome tubulation along microtubules in this work just out from @lysoluis.bsky.social et al:
rupress.org/jcb/article/...

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Reposted by Luis Bonet-Ponce

Rockefeller University Press @rupress.org · 21d

In @jcb.org, @lysoluis.bsky.social, @markrcookson.bsky.social et al. show how two distinct pRAB effectors and RHD members, JIP4 and RILPL1, provide antagonistic motor force to regulate #lysosome tubulation. rupress.org/jcb/article/...

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Reposted by Luis Bonet-Ponce

Journal of Cell Biology @jcb.org · 21d

@lysoluis.bsky.social, @markrcookson.bsky.social et al. show how two distinct pRAB effectors and RHD members, JIP4 and RILPL1, provide antagonistic motor force to regulate #lysosome tubulation. rupress.org/jcb/article/...

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Luis Bonet-Ponce @lysoluis.bsky.social · 21d

Much needed good news!! Our paper is just out in @jcb.org. We found a crazy antagonistic motor function that explains the elongation/retraction phenotype we see during LRRK2-dependent lysosomal tubulation. I am especially proud of Tsion, Nuria, Mia and Irene's contribution. Check it out!

Journal of Cell Biology @jcb.org · 21d

@lysoluis.bsky.social, @markrcookson.bsky.social et al. show how two distinct pRAB effectors and RHD members, JIP4 and RILPL1, provide antagonistic motor force to regulate #lysosome tubulation. rupress.org/jcb/article/...

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Reposted by Luis Bonet-Ponce

Christophe 🔬 L @christlet.bsky.social · 21d

I'm microscope-starved these days so I jumped on the occasion to help @bertrandvernay.bsky.social quality-check our SIM scope at the @ncis-marseille.bsky.social! COS cell stained for DNA 🔵, ER 🟠, mitochondria 🟢, and actin ⚪ (an 8-month old sample!)

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Reposted by Luis Bonet-Ponce

Journal of Cell Biology @jcb.org · 22d

ATG16L strikes again! New findings link #lysosome stress and physiology. Alison Klein and Michael Overholtzer @mskcancercenter.bsky.social discuss new study from @thabataduque.bsky.social et al. of the Deretic group (rupress.org/jcb/article/...) in Spotlight: rupress.org/jcb/article/...

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Reposted by Luis Bonet-Ponce

Zhicheng Cui | 崔志成 @chen3dem.bsky.social · 27d

Finally in @nature.com 🎉
We uncovered step-wise mTORC1 activation on membrane: RAG–Ragulator recruits, RHEB pushes closer, and direct membrane contacts by RAPTOR + mTOR trigger full kinase activity.👉 The membrane is not just a platform — it’s part of the mechanism.
www.nature.com/articles/s41...

Structural basis for mTORC1 activation on the lysosomal membrane - Nature

Cryo-electron microscopy was used to study human mechanistic target of rapamycin complex 1 (mTORC1) activation on lysosomal membranes, showing progressive recruitment by RAG–Ragulator, RHEB and R...

www.nature.com

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Reposted by Luis Bonet-Ponce

Christophe 🔬 L @christlet.bsky.social · 28d

Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11

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Michael Hanna @mike-hanna.bsky.social · Sep 12

New paper alert! I’m very excited to share our paper published in @embojournal.org! @pdc-lab.bsky.social @yaleneuro.bsky.social

The EMBO Journal @embojournal.org · Sep 12

Bridge-like lipid transfer protein 3A (BLTP3A) participates in endomembrane repair as an effector of #ATG8 conjugation to single membranes (CASM) - helping repair or minimize #lysosome damage
@mike-hanna.bsky.social et al, @pdc-lab.bsky.social
www.embopress.org/doi/full/10....

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Reposted by Luis Bonet-Ponce

$pelletfraction.bsky.social$

Cole Sitron @pelletfraction.bsky.social · Sep 10

Why are α-synuclein aggregates in Parkinson’s disease (PD) toxic at the cell biological level?

Our new study shows that α-syn fibrils hijack the ESCRT membrane repair system, triggering a feedback loop that worsens aggregation.

You can find it at: authors.elsevier.com/sd/article/S...

ScienceDirect.com | Science, health and medical journals, full text articles and books.

authors.elsevier.com

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Daniel Krentzel @danielkrentzel.bsky.social · Sep 10

Super happy to see CLEM-Reg out in @natmethods.nature.com! A short thread on the algorithm behind it and how to try it out for yourself🧵(1/9)

www.nature.com/articles/s41...

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy - Nature Methods

CLEM-Reg automates the three-dimensional alignment of volume correlative light and electron microscopy datasets by leveraging probabilistic point cloud registration techniques for fast and accurate re...

www.nature.com

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Lotte B. Pedersen @lottepedersen.bsky.social · Sep 10

KIF13B controls ciliary protein content by promoting endocytic retrieval and suppressing release of large extracellular vesicles from cilia: Current Biology www.cell.com/current-biol...

KIF13B controls ciliary protein content by promoting endocytic retrieval and suppressing release of large extracellular vesicles from cilia

Rezi et al. show that kinesin-3 KIF13B regulates ciliary protein content by promoting endocytic retrieval and small extracellular vesicle (EV) release of ciliary proteins while suppressing their relea...

www.cell.com

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Jonathan Friedman @labfriedman.bsky.social · Sep 3

Thrilled to announce my official promotion to associate professor with tenure at UTSW! So grateful to all the trainees in the lab for their hard work and proud of their accomplishments over the last several years. This an individual recognition but truly a team effort!

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Juan S. Bonifacino @juanbonifacino.bsky.social · Aug 25

Happy to share an exciting study from Yihong Ye’s lab at NIH, with a minor contribution from our lab: ceroid lipofuscinosis-4 (CLN4)-linked DNAJC5 mutations cause lysosomal damage as a driver of neurodegeneration in iPSC-derived neurons. CHIP safeguards lysosomes via microautophagy 👉 rdcu.be/eChof

CHIP protects lysosomes from CLN4 mutant-induced membrane damage

Nature Cell Biology - Lee et al. use an aggregation-prone CLN4 mutant that causes lysosomal damage in neurons and show that in non-neurons, the ubiquitin ligase CHIP prevents CLN4-dependent...

rdcu.be

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Juan S. Bonifacino @juanbonifacino.bsky.social · Aug 21

👉 New publication ahead of print from our lab: tinyurl.com/ye7pntte
We show that the protein SPG21, mutated in hereditary spastic paraplegia 21, localizes to endolysosomes via RAB7A, where it promotes mTORC1-dependent TFEB phosphorylation, reducing expression of a subset of TFEB regulated genes

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