Author | Lightnews

Reposted by Alejandro Adam

Ben Goult @bengoult.bsky.social · 6d

Vinculin is a simple adapter protein right? Wrong!

That’s been the prevailing view for the 46 years since it was discovered.

Here we provide a new paradigm and show that vinculin is a complex mechanosensor itself, containing 6 binary switch domains.

www.science.org/doi/10.1126/...

The mechanical response of vinculin

Vinculin buffers force via dynamic domain unfolding/refolding under physiological load and lacks a stable head-tail lock.

www.science.org

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Alejandro Adam @aleadam.bsky.social · 3d

10/10 EC-specific deletion of STING or a global deletion of STAT1 not only block the interferon-like response, but decrease the severity of the response to LPS

Alejandro Adam @aleadam.bsky.social · 3d

9/10 The transcriptional response involves IRF1, through a mechanism we do not yet fully understand

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Alejandro Adam @aleadam.bsky.social · 3d

8/10 In parallel, IL-6 leads to mitochondrial dysfunction and mtDNA release that leads to cGAS/STING signaling

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Alejandro Adam @aleadam.bsky.social · 3d

7/10 Notably, this response does not require an autocrine IFN signaling, but is a direct transcriptional response to IL-6

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Alejandro Adam @aleadam.bsky.social · 3d

6/10 In vitro work showed that IL-6 leads to an IFN-like response that requires expression of STAT1, but not the canonical STAT3

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Alejandro Adam @aleadam.bsky.social · 3d

5/10 This IFN signature was further increased by the deletion of the IL-6 signaling inhibitor SOCS3 in the endothelium

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Alejandro Adam @aleadam.bsky.social · 3d

4/10 Analysis of the response to LPS suggested a strong interferon activation

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Alejandro Adam @aleadam.bsky.social · 3d

3/10 This enables great enrichment of endothelial RNA

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Alejandro Adam @aleadam.bsky.social · 3d

2/10 To better understand how endothelial cells respond to a cytokine storm, we performed endothelial-specific translating ribosome affinity purification (TRAP) on endotoxemic mice

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Alejandro Adam @aleadam.bsky.social · 3d

1/10 Shock drives vascular dysfunction, with in turn leads to organ injury and potentially death, by inducing a ‘cytokine storm’. Chief among them, IL-6 is an excellent marker for septic shock severity and predicts for mortality

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Alejandro Adam @aleadam.bsky.social · 3d

Here's a quick thread with pics summarizing the paper. Many thanks for all the hard work at Albany Med and by our collaborator Pilar Alcaide!

Alejandro Adam @aleadam.bsky.social · 3d

Very proud of this work that is now out on JCI describing a new #signaling pathway downstream of the #cytokine IL-6 in the #endothelium with implications in #sepsis and #shock. Short thread with a summary coming later today.
For now just enjoy that gorgeous image taken by my student Fatma Awadalla!

Journal of Clinical Investigation @jci.org · 4d

Systemic shock can lead to vascular dysfunction and organ failure.

This issue’s cover features work by Alejandro P. Adam @aleadam.bsky.social showing inflammatory cytokines drives endothelial stress via cytoplasmic DNA: www.jci.org/articles/vie...

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Alejandro Adam @aleadam.bsky.social · 3d

Very proud of this work that is now out on JCI describing a new #signaling pathway downstream of the #cytokine IL-6 in the #endothelium with implications in #sepsis and #shock. Short thread with a summary coming later today.
For now just enjoy that gorgeous image taken by my student Fatma Awadalla!

Journal of Clinical Investigation @jci.org · 4d

Systemic shock can lead to vascular dysfunction and organ failure.

This issue’s cover features work by Alejandro P. Adam @aleadam.bsky.social showing inflammatory cytokines drives endothelial stress via cytoplasmic DNA: www.jci.org/articles/vie...

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Reposted by Alejandro Adam

JMCC Plus @jmccplus.bsky.social · 4d

New work from Daoud et al. shows that removal of YAP/TAZ from vascular smooth muscle cells triggers inflammation and immune activation- indicating these factors as essential in vascular health.#vascularbiology

sciencedirect.com/science/arti...

#CardioSky

YAP/TAZ deletion in vascular smooth muscle cells mirrors atherosclerosis-associated transcriptional programs

The transcriptional co-activators YAP (YAP1) and TAZ (WWTR1) are central regulators of vascular smooth muscle cell (VSMC) phenotype and vascular homeo…

sciencedirect.com

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Reposted by Alejandro Adam

josh farkas 💊 @pulmcrit.bsky.social · 9d

The BICAR-ICU-2 trial just dropped!

RCT involving IV bicarb in metabolic acidosis among patients with mod-severe AKI

Bicarb didn't affect mortality but it reduced the need for dialysis & risk of bacteremia

VERY similar conclusions to BICAR-ICU-1

🧵 #1/

jamanetwork.com/journals/jam... #EMIMCC

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Reposted by Alejandro Adam

neuralstemcells.bsky.social @neuralstemcells.bsky.social · 14d

Stem cell research is opening new doors for treating age-related #MacularDegeneration (AMD), a leading cause of vision loss.

Join our co-founders, Drs. Jeffrey Stern and Sally Temple on @BrightFocus Foundation's Macular Chat on Wed, Oct 29.

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Reposted by Alejandro Adam

Richard McElreath 🐈‍⬛ @rmcelreath.bsky.social · 15d

Monty Python understood p-hacking

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Reposted by Alejandro Adam

Rep. Paul Tonko @reppaultonko.bsky.social · 20d

Today, I’m joining millions of Americans participating in No Kings rallies across the nation to reject Trump’s corruption and cruelty – and to stand up for democracy. Read more in my latest newsletter, and visit nokings.org to find a rally near you:

tonko.house.gov/news/email/s...

Representative Paul D. Tonko

tonko.house.gov

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Reposted by Alejandro Adam

The Histochemical Society (HCS) and Journal (JHC) @histochemicalsoc.bsky.social · 21d

New this month in JHC: "Expression of Glial Cell Line–derived Neurotrophic Factor and Its Receptors in Glioblastoma."

Click to read: bit.ly/3L1NIHz

#glioblastoma #immunohistochemistry #insituhybridization

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Reposted by Alejandro Adam

NY-CURES @nycures.bsky.social · 23d

Add your name ✍ at www.nycures.org so Albany sees 10,000 scientists calling for the future of biomedical research to be built right here in New York.

Our first email to members goes out soon—introducing the statewide biomedical institute we’re proposing and how you can help power it forward.

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Alejandro Adam @aleadam.bsky.social · Oct 6

Impressive demonstration of how a leukocyte separates the two ECs to squeeze by.
Time to update my TEM lecture slides!

preLights @prelights.bsky.social · Oct 6

Excellent #postLight uploaded by @fadelvalle.bsky.social!

He revisits work from Werner van der Meer & colleagues originally posted as a preprint and now published in @cp-cellreports.bsky.social

postLight: prelights.biologists.com/highlights/l...

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Reposted by Alejandro Adam

Max Kozlov @maxkozlov.bsky.social · Sep 30

Staff members at the NIH seem to have done the impossible: spend the agency's $48 billion budget.

“Everyone has been rallying together to clean up the mess, but it’s a mess that did not need to be made,” an NIH program officer told me.

www.nature.com/articles/d41...

NIH races to spend its 2025 grant money — but fewer projects win funding

Despite political obstacles, officials are on track to disburse all of the research funds allocated to US biomedical behemoth.

www.nature.com

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Alejandro Adam @aleadam.bsky.social · Sep 29

@gabbyfredman.bsky.social

EPA+DHA may be considered as part of the management of patients with NYHA functional class II-IV HF, irrespective of etiology or LVEF.

Eric Topol @erictopol.bsky.social · Sep 29

A long-awaited consensus statement on inflammation and heart disease
"We have thus entered an era when the evidence linking inflammation with ASCVD is no longer exploratory but is
compelling and clinically actionable."
@jaccjournals.bsky.social open-access
www.jacc.org/doi/10.1016/...

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Reposted by Alejandro Adam

Stephan Huveneers @huveneerslab.bsky.social · Sep 25

New vascular seminars coming up @evborg.bsky.social

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Reposted by Alejandro Adam

The Onion @theonion.com · Sep 24

Trump Administration Attempts To Link Autism To Tylenol
https://theonion.com/trump-administration-attempts-to-link-autism-to-tylenol/

“I guess it’s back to laudanum.”

Tre Duncan, Sauce Mixer

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