Publication date: Available online 8 January 2026 Source: Acta Tropica Author(s): Matias Pablo Juan Szabó, Adriane Suzin, Alexandre Vogliotti, Rodrigo da Costa Maia, Marcelo B. Labruna

Publication date: Available online 8 January 2026 Source: Acta Tropica Author(s): Eva Bártová, Ali Halajian, Marie Budíková, Alena Žákovská, Heloise Heyne

Publication date: Available online 8 January 2026 Source: Acta Tropica Author(s): Matias Pablo Juan Szabó, Adriane Suzin, Alexandre Vogliotti, Rodrigo da Costa Maia, Marcelo B. Labruna

Publication date: Available online 8 January 2026 Source: Acta Tropica Author(s): Eva Bártová, Ali Halajian, Marie Budíková, Alena Žákovská, Heloise Heyne

Abstract. Ticks present a significant risk to people in the southern United States, particularly those who spend time outdoors, as ticks can transmit agent

Publication date: January 2026 Source: #ticks and #tick-borne Diseases, Volume 17, Issue 1 Author(s): OV Anyango, OW Aool, M Lukindu, T Nakayiki, J Lutwama, JK Kayondo, CD Phillips, J Lutomiah, J Mutisya, CL Brelsfoard, MG Onyango

Publication date: January 2026 Source: #ticks and #tick-borne Diseases, Volume 17, Issue 1 Author(s): OV Anyango, OW Aool, M Lukindu, T Nakayiki, J Lutwama, JK Kayondo, CD Phillips, J Lutomiah, J Mutisya, CL Brelsfoard, MG Onyango

Pathogenic Rickettsia species are obligate intracellular bacteria that must reside in a mammalian host or arthropod vector cell to survive. Although these bacteria transition between different intracellular environments during infection, they encode few putative transcription factors, and their gene regulatory networks are largely unknown. Because of their inextricable relationship with eukaryotic cells, transcriptional profiling of the pathogen is complicated by the abundance of contaminating host RNA, especially in infection conditions or stages where the bacterial burden is inherently low. Here, we employ a hybrid capture technique (PatH-Cap) to improve library preparation by enriching bacterial transcripts while depleting host and rRNA molecules. Using PatH-Cap, we explored transcriptional changes throughout the first 24 hours of infection, including infection initiation - an infection stage which has been difficult to profile with standard library preparation methods. We then clustered genes based on their temporal trends, revealing cohorts of genes whose expression is up- or downregulated at different stages of infection. We also highlighted the diverse temporal expression trends of genes with known roles in growth and pathogenesis, including translation and cell division genes, secreted effectors, and secretion system components. Lastly, we identified 310 antisense RNA molecules, many of which also showed strong temporal trends. This work demonstrates that sensitive transcriptional profiling approaches like PatH-Cap hold great promise for dissecting gene expression networks driving infection in intracellular pathogens that have historically posed significant technical challenges.

Publication date: Available online 3 January 2026 Source: Acta Tropica Author(s): Yuan-Ping Deng, Yi-Liu Liu, Ya Zhang, Yi-Tian Fu, Meng Qi, Guo-Hua Liu

Pathogenic Rickettsia species are obligate intracellular bacteria that must reside in a mammalian host or arthropod vector cell to survive. Although these bacteria transition between different intracellular environments during infection, they encode few putative transcription factors, and their gene regulatory networks are largely unknown. Because of their inextricable relationship with eukaryotic cells, transcriptional profiling of the pathogen is complicated by the abundance of contaminating host RNA, especially in infection conditions or stages where the bacterial burden is inherently low. Here, we employ a hybrid capture technique (PatH-Cap) to improve library preparation by enriching bacterial transcripts while depleting host and rRNA molecules. Using PatH-Cap, we explored transcriptional changes throughout the first 24 hours of infection, including infection initiation - an infection stage which has been difficult to profile with standard library preparation methods. We then clustered genes based on their temporal trends, revealing cohorts of genes whose expression is up- or downregulated at different stages of infection. We also highlighted the diverse temporal expression trends of genes with known roles in growth and pathogenesis, including translation and cell division genes, secreted effectors, and secretion system components. Lastly, we identified 310 antisense RNA molecules, many of which also showed strong temporal trends. This work demonstrates that sensitive transcriptional profiling approaches like PatH-Cap hold great promise for dissecting gene expression networks driving infection in intracellular pathogens that have historically posed significant technical challenges.

Publication date: Available online 3 January 2026 Source: Acta Tropica Author(s): Yuan-Ping Deng, Yi-Liu Liu, Ya Zhang, Yi-Tian Fu, Meng Qi, Guo-Hua Liu

Background Rickettsia typhi is the causative agent of epidemic murine typhus and Rocky Mountain spotted fever. The infection can affect multiple vital organs, including the heart, lungs, kidneys, and ...