Elias Spiliotis
@septinlab.bsky.social
Professor of Cell Biology (University of Virginia), Research Scientist. Science, music, sports and politics. Opinions are my own and not of my lab or university.
Guess no time to digest that turkey. Back on the grant resubmission wagon...
Here we go. Its ALL #NIHGrant applications. Dec 8 is the new deadline. And a one page prelim data update will be accepted for all May Council submissions. grants.nih.gov/grants/guide...
NOT-OD-26-012: Updated Guidance on Reopening of NIH Extramural Activities Following the October 1, 2025 - Lapse in Appropriations
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Updated Guidance on Reopening of NIH Extramural Activities Following the October 1, 2025 - Lapse in Appropriations NOT-...
grants.nih.gov
November 24, 2025 at 8:29 PM
Guess no time to digest that turkey. Back on the grant resubmission wagon...
New septin paper by our next door lab neighbors @ebrahim-lab.bsky.social, and on the cover of JCI Insight no less.
Septin 9 reinforces intestinal epithelial junctions, preventing leaky gut and inflammation.
Check it out!
Septin 9 reinforces intestinal epithelial junctions, preventing leaky gut and inflammation.
Check it out!
This issue’s Cover: @ebrahim-lab.bsky.social @dominikrobak.bsky.social& team report on the septin cytoskeleton at apical junctions of intestinal epithelial cells, whereby it maintains barrier integrity & protects from inflammation: insight.jci.org/articles/vie...
November 24, 2025 at 7:45 PM
New septin paper by our next door lab neighbors @ebrahim-lab.bsky.social, and on the cover of JCI Insight no less.
Septin 9 reinforces intestinal epithelial junctions, preventing leaky gut and inflammation.
Check it out!
Septin 9 reinforces intestinal epithelial junctions, preventing leaky gut and inflammation.
Check it out!
Reposted by Elias Spiliotis
Revealing acute consequences of rapid degradation of synaptic fusion proteins at individual synapses using Auxin-Inducible Degron 2 technology
www.nature.com/articles/s42...
www.nature.com/articles/s42...
November 22, 2025 at 7:59 PM
Revealing acute consequences of rapid degradation of synaptic fusion proteins at individual synapses using Auxin-Inducible Degron 2 technology
www.nature.com/articles/s42...
www.nature.com/articles/s42...
Pavement never broke into 90s alterna/MTV mainstream with their quirky tongue-in-cheek and off kilter song writing, but expanded the borders, possibilities and cred of a newly emerging indie rock DIY scene.
Formed by UVA alums Stephen Malkmus and Scott Kannberg.
www.youtube.com/watch?v=w2Ww...
Formed by UVA alums Stephen Malkmus and Scott Kannberg.
www.youtube.com/watch?v=w2Ww...
Pavement- "Harness Your Hopes" (Official Music Video)
YouTube video by Pavement
www.youtube.com
November 22, 2025 at 12:09 PM
Pavement never broke into 90s alterna/MTV mainstream with their quirky tongue-in-cheek and off kilter song writing, but expanded the borders, possibilities and cred of a newly emerging indie rock DIY scene.
Formed by UVA alums Stephen Malkmus and Scott Kannberg.
www.youtube.com/watch?v=w2Ww...
Formed by UVA alums Stephen Malkmus and Scott Kannberg.
www.youtube.com/watch?v=w2Ww...
"Going forward, ICOs will be considering peer review information in its entirety. NIH ICOs will NOT rely on funding paylines in developing pay plans. Rather, ICOs will consider these scores in context of their and NIH’s priorities, strategic plans, and budgets..."
grants.nih.gov/news-events/...
grants.nih.gov/news-events/...
Implementing a Unified NIH Funding Strategy to Guide Consistent and Clearer Award Decisions | Grants & Funding
grants.nih.gov
November 21, 2025 at 10:51 PM
"Going forward, ICOs will be considering peer review information in its entirety. NIH ICOs will NOT rely on funding paylines in developing pay plans. Rather, ICOs will consider these scores in context of their and NIH’s priorities, strategic plans, and budgets..."
grants.nih.gov/news-events/...
grants.nih.gov/news-events/...
Reposted by Elias Spiliotis
New paper from the lab on a new Gateway compatible toolkit for transgenesis in eukaryotes, led by former technician Will Gillespie.
Open access @dev-journal.bsky.social
journals.biologists.com/dev/article/...
Open access @dev-journal.bsky.social
journals.biologists.com/dev/article/...
MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox for use in vertebrate model systems
Summary: A comprehensive suite of Gateway-compatible plasmids for in vitro and in vivo transgenesis in vertebrate model systems has been developed, together with a relational, modifiable, open-source ...
journals.biologists.com
November 14, 2025 at 5:33 PM
New paper from the lab on a new Gateway compatible toolkit for transgenesis in eukaryotes, led by former technician Will Gillespie.
Open access @dev-journal.bsky.social
journals.biologists.com/dev/article/...
Open access @dev-journal.bsky.social
journals.biologists.com/dev/article/...
Ummmm... whatuh whu?
NIH grant applicants:
October and November grant application submission *deadlines* will be rescheduled (🤯). Specific dates TBD.
grants.nih.gov/grants/guide...
October and November grant application submission *deadlines* will be rescheduled (🤯). Specific dates TBD.
grants.nih.gov/grants/guide...
NOT-OD-26-005: Interim Guidance on Reopening of NIH Extramural Activities
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Interim Guidance on Reopening of NIH Extramural Activities NOT-OD-26-005. NIH
grants.nih.gov
November 15, 2025 at 2:50 AM
Ummmm... whatuh whu?
RIP penny! I guess the all American .99 price gimmick is dead with it too?
www.nytimes.com/2025/11/12/b...
www.nytimes.com/2025/11/12/b...
The Penny Dies at 232
www.nytimes.com
November 12, 2025 at 10:55 PM
RIP penny! I guess the all American .99 price gimmick is dead with it too?
www.nytimes.com/2025/11/12/b...
www.nytimes.com/2025/11/12/b...
Reposted by Elias Spiliotis
One of my science heroes, Joan Brugge, talks about how science funding cuts are slowing their work on breast cancer prevention--share this widely
www.facebook.com/60minutes/vi...
www.facebook.com/60minutes/vi...
439K views · 15K reactions | Harvard researcher Joan Brugge says her work has the potential to prevent breast cancer, but she was notified last spring that her federal funding was terminated. “It was ...
Harvard researcher Joan Brugge says her work has the potential to prevent breast cancer, but she was notified last spring that her federal funding was terminated. “It was just like a gut punch. My...
www.facebook.com
November 10, 2025 at 11:12 AM
One of my science heroes, Joan Brugge, talks about how science funding cuts are slowing their work on breast cancer prevention--share this widely
www.facebook.com/60minutes/vi...
www.facebook.com/60minutes/vi...
Bradknam style!
The new meme for all exciting neuronal cell bio results.
Use in a sentence: My axon extension movies are so sick, I am hopping around the lab Bradknam style.
The new meme for all exciting neuronal cell bio results.
Use in a sentence: My axon extension movies are so sick, I am hopping around the lab Bradknam style.
November 9, 2025 at 10:23 AM
Bradknam style!
The new meme for all exciting neuronal cell bio results.
Use in a sentence: My axon extension movies are so sick, I am hopping around the lab Bradknam style.
The new meme for all exciting neuronal cell bio results.
Use in a sentence: My axon extension movies are so sick, I am hopping around the lab Bradknam style.
6 am ice cream breakfast in Doha, Qatar? Why not!?!
November 9, 2025 at 3:05 AM
6 am ice cream breakfast in Doha, Qatar? Why not!?!
I didn’t expect to run into Gritty in Pune India 😳
November 8, 2025 at 7:27 AM
I didn’t expect to run into Gritty in Pune India 😳
Reposted by Elias Spiliotis
In @jcb.org, Peiyao Fan, Yang Chen et al. @pku1898.bsky.social show that tubular ER extends into retraction fibers and #migrasomes through #microtubule-dependent ER extension and ER-plasma membrane contact sites in migrating cells. rupress.org/jcb/article/...
#ER_literature #Migration #Organelles
#ER_literature #Migration #Organelles
November 6, 2025 at 5:45 PM
In @jcb.org, Peiyao Fan, Yang Chen et al. @pku1898.bsky.social show that tubular ER extends into retraction fibers and #migrasomes through #microtubule-dependent ER extension and ER-plasma membrane contact sites in migrating cells. rupress.org/jcb/article/...
#ER_literature #Migration #Organelles
#ER_literature #Migration #Organelles
In Pune, India for EMBO Cytoskeleton.
Didnt have sitar performance and Filament cocktail bar in my bingo card but… hey!
Come think of it every conference should feature a music performance by a local act and a bar called filament or tubule!
Didnt have sitar performance and Filament cocktail bar in my bingo card but… hey!
Come think of it every conference should feature a music performance by a local act and a bar called filament or tubule!
November 4, 2025 at 6:12 PM
In Pune, India for EMBO Cytoskeleton.
Didnt have sitar performance and Filament cocktail bar in my bingo card but… hey!
Come think of it every conference should feature a music performance by a local act and a bar called filament or tubule!
Didnt have sitar performance and Filament cocktail bar in my bingo card but… hey!
Come think of it every conference should feature a music performance by a local act and a bar called filament or tubule!
UVA Cell Biology got talent!
Happy Halloween 🎃
Happy Halloween 🎃
October 31, 2025 at 2:16 PM
UVA Cell Biology got talent!
Happy Halloween 🎃
Happy Halloween 🎃
Happy Halloween y’all… from the victors of the Creepiest award of the 2025 Spooktacular Carving Contest
October 31, 2025 at 2:12 PM
Happy Halloween y’all… from the victors of the Creepiest award of the 2025 Spooktacular Carving Contest
Reposted by Elias Spiliotis
Today @anthropic.com released PubMed integration for Claude. No hallucinations. Just real science, real data. As a beta tester, this has been game changing— a supercharged research tool. Here are 6 prompts that will transform how you search the literature. A 🧵
www.anthropic.com/news/claude-...
www.anthropic.com/news/claude-...
Claude for Life Sciences
Discover how Claude accelerates life sciences research with new scientific connectors, skills, and improved performance for drug discovery and clinical work.
www.anthropic.com
October 20, 2025 at 5:27 PM
Today @anthropic.com released PubMed integration for Claude. No hallucinations. Just real science, real data. As a beta tester, this has been game changing— a supercharged research tool. Here are 6 prompts that will transform how you search the literature. A 🧵
www.anthropic.com/news/claude-...
www.anthropic.com/news/claude-...
Do not fold, do not sign
October 17, 2025 at 8:11 PM
Do not fold, do not sign
Ooo-la-la!
Learn all things #cytoskeleton #research in an interdisciplinary environment at EMBO Workshop "Structure and Function of the Cytoskeleton" in Paris, FR, 7–10 April 2026.
Deadline: 20 December 2025
https://meetings.embo.org/event/26-cytoskeleton
#EMBOCytoskeletalFunction #EMBOevents #conference 🧪
Deadline: 20 December 2025
https://meetings.embo.org/event/26-cytoskeleton
#EMBOCytoskeletalFunction #EMBOevents #conference 🧪
Structure and Function of the Cytoskeleton
The cytoskeleton, a dynamic and intricate network of protein filaments, is found within the cytoplasm of all cells, from bacteria and archaea to complex eukaryotes. This essential cellular component …
meetings.embo.org
October 7, 2025 at 11:16 PM
Ooo-la-la!
Reposted by Elias Spiliotis
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
October 2, 2025 at 5:11 PM
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
Exciting news!
Submissions are now open for the first @ascbiology.bsky.social #MBOC Spercial Issue on #Septins
Submission deadline: April 30, 2026
RT and spread the word at #CellBio2025
www.molbiolcell.org/cell-biology...
Submissions are now open for the first @ascbiology.bsky.social #MBOC Spercial Issue on #Septins
Submission deadline: April 30, 2026
RT and spread the word at #CellBio2025
www.molbiolcell.org/cell-biology...
October 1, 2025 at 8:43 PM
Exciting news!
Submissions are now open for the first @ascbiology.bsky.social #MBOC Spercial Issue on #Septins
Submission deadline: April 30, 2026
RT and spread the word at #CellBio2025
www.molbiolcell.org/cell-biology...
Submissions are now open for the first @ascbiology.bsky.social #MBOC Spercial Issue on #Septins
Submission deadline: April 30, 2026
RT and spread the word at #CellBio2025
www.molbiolcell.org/cell-biology...
NYTimes biopic and chronicle on Stephen Starr who can be credited for the start of Philly's culinary scene.
Good source of restaurant names for your Philly ASCB lab dinners.
www.nytimes.com/2025/09/30/d...
Good source of restaurant names for your Philly ASCB lab dinners.
www.nytimes.com/2025/09/30/d...
The $400 Million Restaurant Man
www.nytimes.com
October 1, 2025 at 2:05 PM
NYTimes biopic and chronicle on Stephen Starr who can be credited for the start of Philly's culinary scene.
Good source of restaurant names for your Philly ASCB lab dinners.
www.nytimes.com/2025/09/30/d...
Good source of restaurant names for your Philly ASCB lab dinners.
www.nytimes.com/2025/09/30/d...
Reposted by Elias Spiliotis
Happy to share the inaugural paper from the lab. We describe a molecular mechanism for the activation of outer dynein arm motors that power the vital motion of cilia.
Open access link below:
www.nature.com/articles/s41...
Here's a cool animated summary
Open access link below:
www.nature.com/articles/s41...
Here's a cool animated summary
September 29, 2025 at 7:09 PM
Happy to share the inaugural paper from the lab. We describe a molecular mechanism for the activation of outer dynein arm motors that power the vital motion of cilia.
Open access link below:
www.nature.com/articles/s41...
Here's a cool animated summary
Open access link below:
www.nature.com/articles/s41...
Here's a cool animated summary
👏
The fiscal year ends tomorrow.
Here are results from NIH Reporter downloaded an hour ago.
The total amount of funding committed for FY2025 at this point is 99.0% of that for FY2024. The same difference could be due to a variety of technical factors.
1/3
Here are results from NIH Reporter downloaded an hour ago.
The total amount of funding committed for FY2025 at this point is 99.0% of that for FY2024. The same difference could be due to a variety of technical factors.
1/3
September 29, 2025 at 11:24 PM
👏