While everyone was having a listen to and a laugh at Barnaby Joyce over his push to repeal net-zero laws,…

Support Belinda to study a Masters in Infectious disease and One Heal… Belinda Omino needs your support for Support Belinda to study her Masters in One Health

Infectious Diseases and One Health (IDOH) is a 2-year full time ERASMUS Mundus Joint Master Degree. Mobility includes France (Tours), Spain (Barcelona) and Germany (Hannover).

Infectious Diseases and One Health (IDOH) is a 2-year full time ERASMUS Mundus Joint Master Degree. Mobility includes France (Tours), Spain (Barcelona) and Germany (Hannover).

AbstractBackground. The escalating global threat of antimicrobial resistance (AMR) necessitates the development of novel antimicrobial agents, innovative s

AbstractBackground. The escalating global threat of antimicrobial resistance (AMR) necessitates the development of novel antimicrobial agents, innovative s

The escalating global threat of antimicrobial resistance (AMR) necessitates the development of novel antimicrobial agents, innovative strategies, and representative infection models to combat AMR bacterial infections. Host defence peptides (HDPs) and their derivatives have been proposed as complements to conventional antibiotics due to their antibacterial activity and modulation of the immune response.ObjectivesThis study investigated the novel use of the HDP-derived synthetic antibacterial and anti-biofilm peptide (SAAP)-148 as a pretreatment in epithelial tissue models to prevent colonization by AMR bacteria. The combined activities of SAAP-148 pretreatment with post-infection halicin to treat infections were also explored.MethodsEmploying cultured human skin equivalents (HSEs) and primary bronchial epithelial cells (PBECs) as models of tissue infection, we examined the prophylactic and therapeutic effects of SAAP-148, both singularly and in combination with the repurposed antibiotic halicin, against AMR bacteria. We additionally interrogated the response of HSE and PBEC cultures to SAAP-148 treatment via confocal microscopy and quantitative PCR of native HDPs and inflammatory cytokine genes.ResultsOur findings demonstrated that pretreatment with SAAP-148 significantly reduces colonization of HSEs and PBECs by AMR Staphylococcus aureus and Pseudomonas aeruginosa. Confocal microscopy revealed differential uptake and localization of SAAP-148 in these tissues, correlating with its distinct activity in these tissues. SAAP-148 exposure temporarily increased expression of the HDPs cathelicidin (CAMP) and β-defensin 1 (DEFB1), and the cytokine IL-8 (CXCL8), which did not correlate with the transient antibacterial activity observed. Sequential treatment with SAAP-148 prior to infection with AMR S. aureus and post-infection halicin treatment demonstrated synergistic activity in HSEs, whereas this combined activity was indifferent in PBEC cultures.ConclusionsThese results support SAAP-148 as a candidate for pre-infection prophylaxis and synergistic antibiotic therapy with halicin in skin, broadening the potential of both agents to address AMR bacterial infection.

The deputy director of a liberal project at Yale Law School was put on leave over allegations that she is linked to Samidoun, a group the U.S. government has said funds terrorists.

A staffer for Elon Musk’s Department of Government Efficiency whose access to U.S. Treasury payment systems was approved by a federal judge on Thursday has links to a deleted social-media account that...