Nitish Dua
@nitishdua.bsky.social
Post-Doc in Grotjahn lab (http://grotjahnlab.org) @scrippsresearch. Previously, PhD in Badrinarayanan lab (http://microgenomes.com) @NCBS_bangalore . 🏳️🌈
#mitochondria #cryoET
#mitochondria #cryoET
Reposted by Nitish Dua
Just installed Doppio on our Linux machine. This is awesome work making relion more accessible and easy to run. I haven't tried submitting jobs yet but it is already making our live easier!
I met Matt Iadanza at the CZI but I guess he doesn't have bluesky to tag!
I met Matt Iadanza at the CZI but I guess he doesn't have bluesky to tag!
November 25, 2025 at 6:26 PM
Just installed Doppio on our Linux machine. This is awesome work making relion more accessible and easy to run. I haven't tried submitting jobs yet but it is already making our live easier!
I met Matt Iadanza at the CZI but I guess he doesn't have bluesky to tag!
I met Matt Iadanza at the CZI but I guess he doesn't have bluesky to tag!
Reposted by Nitish Dua
An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice @natcomms.nature.com @ryanlab.bsky.social @tcddublin.bsky.social @universitypress.cambridge.org
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 20, 2025 at 3:30 PM
An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice @natcomms.nature.com @ryanlab.bsky.social @tcddublin.bsky.social @universitypress.cambridge.org
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Nitish Dua
CryoSift is now out @actacrystf.iucr.org doi.org/10.1107/S205... Thanks to all involved. Happy processing!
November 7, 2025 at 4:58 PM
CryoSift is now out @actacrystf.iucr.org doi.org/10.1107/S205... Thanks to all involved. Happy processing!
Exciting !
BRET-Based Mitochondrial Subcompartment Localization Biosensors https://www.biorxiv.org/content/10.1101/2025.10.27.684728v1
October 28, 2025 at 9:13 PM
Exciting !
Reposted by Nitish Dua
I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)
May 23, 2025 at 7:57 PM
I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)
Reposted by Nitish Dua
mStayRose is published in JBC 🥳🎉
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
StayRose: a photostable StayGold derivative red-shifted by genetic code expansion
Photobleaching of fluorescent proteins often limits the acquisition of high-quality
images in microscopy. StayGold, a novel dimeric green fluorescent protein recently
monomerised through sequence engi...
www.jbc.org
October 22, 2025 at 4:58 PM
mStayRose is published in JBC 🥳🎉
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
Reposted by Nitish Dua
Last weeks #immunometabolism discoveries @biomednews.bsky.social ⬇️⬇️⬇️
biomed.news/bims-imicid/...
Lots of excellent work inside incl.
Backtracking metabolic dynamics in single cells predicts bacterial replication in human macrophages.
biomed.news/bims-imicid/...
Lots of excellent work inside incl.
Backtracking metabolic dynamics in single cells predicts bacterial replication in human macrophages.
October 20, 2025 at 12:27 PM
Last weeks #immunometabolism discoveries @biomednews.bsky.social ⬇️⬇️⬇️
biomed.news/bims-imicid/...
Lots of excellent work inside incl.
Backtracking metabolic dynamics in single cells predicts bacterial replication in human macrophages.
biomed.news/bims-imicid/...
Lots of excellent work inside incl.
Backtracking metabolic dynamics in single cells predicts bacterial replication in human macrophages.
Reposted by Nitish Dua
Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase
Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...
www.science.org
October 16, 2025 at 7:17 PM
Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Nitish Dua
Reposted by Nitish Dua
Feeling mitochondrially challenged lately?
Here are two classic reviews on #mitochondria that should help.
www.cell.com/current-biol...
www.cell.com/current-biol...
Here are two classic reviews on #mitochondria that should help.
www.cell.com/current-biol...
www.cell.com/current-biol...
The Origin and Diversification of Mitochondria
Moving beyond the simplistic view of mitochondria as the power house of the cell,
Roger et al. present the immense diversity of mitochondria and mitochondrial functions
across eukaryotes, and bring th...
www.cell.com
August 28, 2025 at 10:28 PM
Feeling mitochondrially challenged lately?
Here are two classic reviews on #mitochondria that should help.
www.cell.com/current-biol...
www.cell.com/current-biol...
Here are two classic reviews on #mitochondria that should help.
www.cell.com/current-biol...
www.cell.com/current-biol...
📣📣📣📣
🚨 #teamtomo!🚨
Did you know there's a dedicated cryoEM/ET Mini-Symposium at the upcoming ASCB/EMBO meeting in Philly (Dec 6–10)??
Did you know there's a dedicated cryoEM/ET Mini-Symposium at the upcoming ASCB/EMBO meeting in Philly (Dec 6–10)??
August 24, 2025 at 4:15 AM
📣📣📣📣
Reposted by Nitish Dua
Kailash Venkatraman, Nicolas-Frédéric Lipp & @ibudin.bsky.social examine the similarities between prokaryotic intracytoplasmic membranes & mitochondrial IMs, & discuss whether cristae evolution has driven specialisation of the #mito lipidome.
journals.biologists.com/jcs/article/...
#JCSMitoSI
journals.biologists.com/jcs/article/...
#JCSMitoSI
May 12, 2025 at 9:05 AM
Kailash Venkatraman, Nicolas-Frédéric Lipp & @ibudin.bsky.social examine the similarities between prokaryotic intracytoplasmic membranes & mitochondrial IMs, & discuss whether cristae evolution has driven specialisation of the #mito lipidome.
journals.biologists.com/jcs/article/...
#JCSMitoSI
journals.biologists.com/jcs/article/...
#JCSMitoSI
Reposted by Nitish Dua
I had some absolutely incredible papers come online this week, and I need to take a minute to post about each of them. First, let me tell you about the CarboTag probes for imaging plant cell walls! From the Sprakel lab (a short thread) www.nature.com/articles/s41...
May 1, 2025 at 8:36 PM
I had some absolutely incredible papers come online this week, and I need to take a minute to post about each of them. First, let me tell you about the CarboTag probes for imaging plant cell walls! From the Sprakel lab (a short thread) www.nature.com/articles/s41...
Reposted by Nitish Dua
So excited for this work to be out!! Thanks to @attychang.bsky.social, @hamid13r.bsky.social, blue sky less Daniel Fuentes, @nanigrotjahn.bsky.social and @tomo.science!!!
🚨New preprint!🚨 #teamtomo
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.
Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.
Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
Surface morphometrics reveals local membrane thickness variation in organellar subcompartments https://www.biorxiv.org/content/10.1101/2025.04.30.651574v1
May 1, 2025 at 8:54 PM
So excited for this work to be out!! Thanks to @attychang.bsky.social, @hamid13r.bsky.social, blue sky less Daniel Fuentes, @nanigrotjahn.bsky.social and @tomo.science!!!
Reposted by Nitish Dua
🚨New preprint!🚨 #teamtomo
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.
Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.
Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
Surface morphometrics reveals local membrane thickness variation in organellar subcompartments https://www.biorxiv.org/content/10.1101/2025.04.30.651574v1
May 1, 2025 at 7:42 PM
🚨New preprint!🚨 #teamtomo
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.
Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.
Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
Reposted by Nitish Dua
#invertefest needs some more marine representation, so here are 2 Liriope sp. hydrozoan and a teeny ctenophore amongst numerous copepods. Data recorded (5 fps) via a benchtop shadowgraph camera from a non-filtering end plankton net tow.
April 25, 2025 at 4:59 PM
#invertefest needs some more marine representation, so here are 2 Liriope sp. hydrozoan and a teeny ctenophore amongst numerous copepods. Data recorded (5 fps) via a benchtop shadowgraph camera from a non-filtering end plankton net tow.
Reposted by Nitish Dua
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
April 11, 2025 at 8:35 AM
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
Reposted by Nitish Dua
I know print journals aren't exactly on everyone's radar these days, but we’re still geeking out that our latest study—led by the brilliant @attychang.bsky.social—landed the cover of the April issue of JCB! 🤩
@zidlab.bsky.social @tomo.science @hamid13r.bsky.social
@zidlab.bsky.social @tomo.science @hamid13r.bsky.social
Our April issue is here! rupress.org/jcb/issue/22...
The cover shows a segmented model of cytoplasmic #ribosomes associated with mitochondrial membrane in a Saccharomyces cerevisiae cell. From Ya-Ting Chang, @nanigrotjahn.bsky.social and colleagues (doi.org/10.1083/jcb....)
The cover shows a segmented model of cytoplasmic #ribosomes associated with mitochondrial membrane in a Saccharomyces cerevisiae cell. From Ya-Ting Chang, @nanigrotjahn.bsky.social and colleagues (doi.org/10.1083/jcb....)
April 9, 2025 at 10:35 PM
I know print journals aren't exactly on everyone's radar these days, but we’re still geeking out that our latest study—led by the brilliant @attychang.bsky.social—landed the cover of the April issue of JCB! 🤩
@zidlab.bsky.social @tomo.science @hamid13r.bsky.social
@zidlab.bsky.social @tomo.science @hamid13r.bsky.social
Reposted by Nitish Dua
Happy to share Asha Joseph and Inchara Adhikashreni's latest work from the lab, describing replisome speed variation in Caulobacter!
www.cell.com/current-biol...
www.cell.com/current-biol...
Live tracking of replisomes reveals nutrient-dependent regulation of replication elongation rates in Caulobacter crescentus
Adhikashreni et al. use quantitative live-cell imaging to track cell-cycle dynamics
in hundreds of individual bacteria. They observe nutrient-dependent regulation of
replication elongation rates that ...
www.cell.com
April 4, 2025 at 3:57 PM
Happy to share Asha Joseph and Inchara Adhikashreni's latest work from the lab, describing replisome speed variation in Caulobacter!
www.cell.com/current-biol...
www.cell.com/current-biol...
Reposted by Nitish Dua
Excited to share our latest paper in Science Advances! We finally dissected a new pathway for mitochondrial quality control upon mtDNA replication stress! Let’s start a short thread! 🧵
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Retromer promotes the lysosomal turnover of mtDNA
Lysosomal uptake of mtDNA controls mitochondrial quality.
www.science.org
April 4, 2025 at 6:42 PM
Excited to share our latest paper in Science Advances! We finally dissected a new pathway for mitochondrial quality control upon mtDNA replication stress! Let’s start a short thread! 🧵
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Nitish Dua
1/ Transposable elements are often called "jumping genes" because they mobilize within genomes. 🧬
But did you know they can also jump 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 cells? 🤯
Our new study reveals how retrotransposons invade the germline directly from somatic cells.
www.biorxiv.org/content/10.1...
A short thread 🧵👇
But did you know they can also jump 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 cells? 🤯
Our new study reveals how retrotransposons invade the germline directly from somatic cells.
www.biorxiv.org/content/10.1...
A short thread 🧵👇
March 17, 2025 at 11:56 AM
1/ Transposable elements are often called "jumping genes" because they mobilize within genomes. 🧬
But did you know they can also jump 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 cells? 🤯
Our new study reveals how retrotransposons invade the germline directly from somatic cells.
www.biorxiv.org/content/10.1...
A short thread 🧵👇
But did you know they can also jump 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 cells? 🤯
Our new study reveals how retrotransposons invade the germline directly from somatic cells.
www.biorxiv.org/content/10.1...
A short thread 🧵👇
Reposted by Nitish Dua
At @standupforscience.bsky.social and the signs are statistically significantly excellent
March 7, 2025 at 6:51 PM
At @standupforscience.bsky.social and the signs are statistically significantly excellent
Reposted by Nitish Dua
Happy to share that the final version of record for our (
@nanigrotjahn.bsky.social) #teamtomo mitochondrial cotranslation story is online at @jcb.org! We combined STA with surface morphometrics to learn how ribosomes bind to and alter mitochondrial membranes in yeast.
rupress.org/jcb/article/...
@nanigrotjahn.bsky.social) #teamtomo mitochondrial cotranslation story is online at @jcb.org! We combined STA with surface morphometrics to learn how ribosomes bind to and alter mitochondrial membranes in yeast.
rupress.org/jcb/article/...
March 7, 2025 at 12:32 AM
Happy to share that the final version of record for our (
@nanigrotjahn.bsky.social) #teamtomo mitochondrial cotranslation story is online at @jcb.org! We combined STA with surface morphometrics to learn how ribosomes bind to and alter mitochondrial membranes in yeast.
rupress.org/jcb/article/...
@nanigrotjahn.bsky.social) #teamtomo mitochondrial cotranslation story is online at @jcb.org! We combined STA with surface morphometrics to learn how ribosomes bind to and alter mitochondrial membranes in yeast.
rupress.org/jcb/article/...
Reposted by Nitish Dua
🚀 Excited to share our latest work on Mrx6 and mtDNA copy number regulation, now available on @biorxivpreprint.bsky.social ! We uncover how Mrx6 and Mam33 interact with the Lon protease Pim1 to control substrate degradation. 🔬 Check it out here: www.biorxiv.org/content/10.1... #mitochondria #mtDNA
Mrx6 binds the Lon protease Pim1 N-terminal domain to confer selective substrate specificity and regulate mtDNA copy number
Mitochondrial DNA (mtDNA) copy number regulation remains incompletely understood, despite its importance in cellular function. In Saccharomyces cerevisiae, Mrx6 belongs to the Pet20-domain-containing ...
www.biorxiv.org
February 16, 2025 at 2:04 PM
🚀 Excited to share our latest work on Mrx6 and mtDNA copy number regulation, now available on @biorxivpreprint.bsky.social ! We uncover how Mrx6 and Mam33 interact with the Lon protease Pim1 to control substrate degradation. 🔬 Check it out here: www.biorxiv.org/content/10.1... #mitochondria #mtDNA
Reposted by Nitish Dua
Had the incredible opportunity to present my work at #EMBOinsitu workshop in Heidelberg. It was filled with fantastic science and new friends! #teamtomo
February 8, 2025 at 1:00 PM
Had the incredible opportunity to present my work at #EMBOinsitu workshop in Heidelberg. It was filled with fantastic science and new friends! #teamtomo