Author | Lightnews

Ben Barad @baradlab.com · 11d

It's really lovely so far - faster and cleaner than deepdewedge on the in vitro sample I've seen it on.

Reposted by Ben Barad

Florian Schur @schurlab.bsky.social · 19d

ISTA @istaresearch.bsky.social is hiring a tenure-track staff scientist in Computational Structural Biology, to develop tools for cryo-EM, cryo-ET, structural prediction methods and protein interaction analysis!

Please spread the word!

ista.ac.at/en/job/tenur...

Tenure-based Research Staff Scientist Position in Computational Structural Biology

ISTA (www.ista.ac.at) is seeking a highly skilled Staff Scientist in Computational Structural Biology to support and advance the Institute’s structural biology research through the development and imp...

ista.ac.at

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Reposted by Ben Barad

Bronwyn Lucas @bronwynalucas.bsky.social · Aug 30

First pre-print from the Lucas Lab led by Matthew Giammar and @joshdcryoem.bsky.social online today! We develop a new, extensible Python implementation of 2DTM and apply it to build pixel size optimization, constrained search and characterize molecular motions in situ www.biorxiv.org/content/10.1...

Leopard-EM: An extensible 2DTM package to accelerate in situ structural biology

The ability to generate high-resolution views of cells with cryogenic electron microscopy (cryo-EM) can reveal the molecular mechanisms of biological processes in their native cellular context. The re...

www.biorxiv.org

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Reposted by Ben Barad

Katie Downes @dr-downes.bsky.social · Jul 30

Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!!

Make my day and check it out!
www.biorxiv.org/content/10.1...

#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬

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Ben Barad @baradlab.com · Jul 9

Very cool that it works this way!

Ben Barad @baradlab.com · Jun 11

This is obviously outrageous, but also thank you for the service you did before it got ripped away!

Reposted by Ben Barad

Bella Rauch @rauchlab.bsky.social · Jun 6

I'm at the OHSU PREP research symposium and I am so impressed with our trainees, they present and know their project background better then many a graduate student. All the more I'm filled with rage about the cancellation of this wonderful #NIH program 🤬

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Ben Barad @baradlab.com · May 7

Wild but also somehow fair that the Thorns don't move up after a pretty clean win against the former #1 ranked team. So much uncertainty still.

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Ben Barad @baradlab.com · May 7

Anti-gatekeeping workflows is what keeps #teamtomo growing! Love to see it.

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Ben Barad @baradlab.com · May 6

Beautiful work and such a cool mechanism!

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Ben Barad @baradlab.com · May 3

A result that was so cool we had to see more how things looked the rest of the time!

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Ben Barad @baradlab.com · May 3

They are actually still attached to the ER with continuous membrane (and a macromolecular assembly that keeps them budded) which makes it even crazier - presumably there is still lipid exchange.

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Ben Barad @baradlab.com · May 2

Its easy to imagine there being some repetitive artifact in time series data but not quite THAT identical!

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Ben Barad @baradlab.com · May 2

So far, no organelle we've seen comes close to the replication organelles from langatvirus infections: www.biorxiv.org/content/10.1...

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Ben Barad @baradlab.com · May 1

More than beta testing, the flavivirus story is what got us into this in general! Not to mention the existing work of Fred Heberle, Ilya Levental, and Neal Waxham that inspired the actual density scanning and fitting approach we use.

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Ben Barad @baradlab.com · Apr 29

Local workstation is our (imperfect) solution...

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Ben Barad @baradlab.com · Apr 18

Hard launch for the Park lab at Scripps too for the tomo!

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Reposted by Ben Barad

Danielle Grotjahn @nanigrotjahn.bsky.social · Apr 9

I know print journals aren't exactly on everyone's radar these days, but we’re still geeking out that our latest study—led by the brilliant @attychang.bsky.social—landed the cover of the April issue of JCB! 🤩

@zidlab.bsky.social @tomo.science @hamid13r.bsky.social

Journal of Cell Biology @jcb.org · Apr 7

Our April issue is here! rupress.org/jcb/issue/22...
The cover shows a segmented model of cytoplasmic #ribosomes associated with mitochondrial membrane in a Saccharomyces cerevisiae cell. From Ya-Ting Chang, @nanigrotjahn.bsky.social and colleagues (doi.org/10.1083/jcb....)

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Ben Barad @baradlab.com · Apr 3

Marketing aside, that 20 micron AFIS range does look pretty appealing for imaging big lamellae!

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Ben Barad @baradlab.com · Mar 28

Her grandma was in the merch shop before the game last week getting a jersey and telling everyone how excited she was for the home debut - so very sweet!

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Reposted by Ben Barad

James Fraser @fraserlab.com · Mar 16

Since @stephanieaw.bsky.social left the lab to start her group at Vanderbilt, we've been talking regularly on the ups and downs of academic science. We started recording...

The Tortured Proteins Department

open.spotify.com/show/10PHVou...

(science with a small dose of running and Taylor Swift)

The Tortured Proteins Department

Podcast · Stephanie Wankowicz · A deep dive into scientific discovery and the process of doing science, hosted by Stephanie Wankowicz and James Fraser.

open.spotify.com

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Ben Barad @baradlab.com · Mar 16

Dual axis stage vs some sharpie marks, who comes out ahead?

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Ben Barad @baradlab.com · Mar 9

Thanks! Whoever manages to recruit Atty (ya-ting) as a postdoc is going to be living it up - she led sample prep, data collection, processing, and all of the new morphometrics programming.

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Ben Barad @baradlab.com · Mar 8

I want one

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Reposted by Ben Barad

Danielle Grotjahn @nanigrotjahn.bsky.social · Mar 7

For #teamtomo, context is everything.

We highlight the power of #surfacemorphometrics in guiding STA and quantitatively assessing the impact of these structures on the local environment.

1st collab paper w/ @zidlab.bsky.social (hopefully many more to come!)

@tomo.science @hamid13r.bsky.social

Journal of Cell Biology @jcb.org · Mar 6

Ya-Ting Chang, @nanigrotjahn.bsky.social et al. @scripps.edu present a membrane-guided approach for identifying a subset of cytoplasmic #ribosomes oriented for protein import on the surface of #mitochondria in budding yeast using #cryoET: rupress.org/jcb/article/...

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