Kathrin Lang
@klanglab.bsky.social
Prof for chemical biology at ETH Zurich
Interested in: genetic code expansion - chembio tools - bioorthogonal chemistries - PTMs - Ub in all its shades - protein engineering
Interested in: genetic code expansion - chembio tools - bioorthogonal chemistries - PTMs - Ub in all its shades - protein engineering
Pinned
Kathrin Lang
@klanglab.bsky.social
· Oct 16
Hijacking a bacterial ABC transporter for genetic code expansion - Nature
Bacterial ATP-binding cassette (ABC) transporters can be utilized and engineered to transport non-canonical amino acids into Escherichia coli for highly efficient synthesis of proteins with novel func...
www.nature.com
🚨Our paper is out! 🥳
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Reposted by Kathrin Lang
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
October 31, 2025 at 9:24 AM
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
How selective is your favourite electrophile? 🤔 🧪 🧫 🎯
Check out this thorough study from the @stephanhacker2.bsky.social lab, now published in Nature Chemistry!
Many congrats to everyone. We are thrilled to have contributed a little! Many congrats to @klanglab.bsky.social alumni Kristina and Marko!
Check out this thorough study from the @stephanhacker2.bsky.social lab, now published in Nature Chemistry!
Many congrats to everyone. We are thrilled to have contributed a little! Many congrats to @klanglab.bsky.social alumni Kristina and Marko!
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)
www.nature.com/articles/s41...
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...
www.nature.com
October 30, 2025 at 2:39 PM
How selective is your favourite electrophile? 🤔 🧪 🧫 🎯
Check out this thorough study from the @stephanhacker2.bsky.social lab, now published in Nature Chemistry!
Many congrats to everyone. We are thrilled to have contributed a little! Many congrats to @klanglab.bsky.social alumni Kristina and Marko!
Check out this thorough study from the @stephanhacker2.bsky.social lab, now published in Nature Chemistry!
Many congrats to everyone. We are thrilled to have contributed a little! Many congrats to @klanglab.bsky.social alumni Kristina and Marko!
Reposted by Kathrin Lang
Super proud of this story! ☺️ Huge thanks to @taruniype.bsky.social for the amazing work - and to @klanglab.bsky.social for the constant support! The first time we saw that phenomenon actually goes all the way back to my Master’s thesis in 2016 🫣 Every now and then, persistence pays off 🤞
🚨Our paper is out! 🥳
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for genetic code expansion - Nature
Bacterial ATP-binding cassette (ABC) transporters can be utilized and engineered to transport non-canonical amino acids into Escherichia coli for highly efficient synthesis of proteins with novel func...
www.nature.com
October 16, 2025 at 3:20 PM
Super proud of this story! ☺️ Huge thanks to @taruniype.bsky.social for the amazing work - and to @klanglab.bsky.social for the constant support! The first time we saw that phenomenon actually goes all the way back to my Master’s thesis in 2016 🫣 Every now and then, persistence pays off 🤞
🚨Our paper is out! 🥳
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for genetic code expansion - Nature
Bacterial ATP-binding cassette (ABC) transporters can be utilized and engineered to transport non-canonical amino acids into Escherichia coli for highly efficient synthesis of proteins with novel func...
www.nature.com
October 16, 2025 at 2:06 PM
🚨Our paper is out! 🥳
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Reposted by Kathrin Lang
new preprint: Ubiquitin is a protein modification linked with degradation but known to regulate other functions. Over 100k ubiquitination sites have been discovered and here we (@julianvangerwen.bsky.social + others) try to prioritize those most critical to the cell www.biorxiv.org/content/10.1...
The functional landscape of the human ubiquitinome
Protein ubiquitination regulates cell biology through diverse avenues, from quality control-linked protein degradation to signaling functions such as modulating protein-protein interactions and enzyme...
www.biorxiv.org
October 9, 2025 at 7:07 AM
new preprint: Ubiquitin is a protein modification linked with degradation but known to regulate other functions. Over 100k ubiquitination sites have been discovered and here we (@julianvangerwen.bsky.social + others) try to prioritize those most critical to the cell www.biorxiv.org/content/10.1...
Reposted by Kathrin Lang
Today, I would like to honor the memory of Roger Y. Tsien, who died on August 24, 2016. His legacy lives with all who use his technologies, including calcium sensors, fluorescent proteins, the acetoxymethyl (AM) ester, & many more! #FluorescenceFriday
www.nature.com/articles/nme...
www.nature.com/articles/nme...
August 22, 2025 at 1:17 PM
Today, I would like to honor the memory of Roger Y. Tsien, who died on August 24, 2016. His legacy lives with all who use his technologies, including calcium sensors, fluorescent proteins, the acetoxymethyl (AM) ester, & many more! #FluorescenceFriday
www.nature.com/articles/nme...
www.nature.com/articles/nme...
Reposted by Kathrin Lang
1/16 New pre-print from the Sternberg Lab!
We uncover how temperate phages can use RNA-guided transcription factors to remodel the flagellar composition of their bacterial host and enhance their fitness.
Find the preprint and full story here: tinyurl.com/mshwjd77
We uncover how temperate phages can use RNA-guided transcription factors to remodel the flagellar composition of their bacterial host and enhance their fitness.
Find the preprint and full story here: tinyurl.com/mshwjd77
July 24, 2025 at 7:58 PM
1/16 New pre-print from the Sternberg Lab!
We uncover how temperate phages can use RNA-guided transcription factors to remodel the flagellar composition of their bacterial host and enhance their fitness.
Find the preprint and full story here: tinyurl.com/mshwjd77
We uncover how temperate phages can use RNA-guided transcription factors to remodel the flagellar composition of their bacterial host and enhance their fitness.
Find the preprint and full story here: tinyurl.com/mshwjd77
Reposted by Kathrin Lang
Reposted by Kathrin Lang
New Preprint: We have solved the structure of the proteasome in complex with two different ubiquitin chains: www.biorxiv.org/content/10.1... This work is spearheaded by PhD Student Sascha Amann
Let me walk you through it 🧵 1/9
Let me walk you through it 🧵 1/9
Structural basis for the ubiquitin chain recognition of the human 26S proteasome
Proteasomal degradation is a fundamental process for all eukaryotic life. A protein destined for degradation is first tagged with a polyubiquitin chain, which is selected by the proteasome. Different ...
www.biorxiv.org
April 9, 2025 at 8:59 AM
New Preprint: We have solved the structure of the proteasome in complex with two different ubiquitin chains: www.biorxiv.org/content/10.1... This work is spearheaded by PhD Student Sascha Amann
Let me walk you through it 🧵 1/9
Let me walk you through it 🧵 1/9
Reposted by Kathrin Lang
Nature research paper: A broad-spectrum lasso peptide antibiotic targeting the bacterial ribosome
https://go.nature.com/4iECjJz
https://go.nature.com/4iECjJz
A broad-spectrum lasso peptide antibiotic targeting the bacterial ribosome - Nature
A new lasso peptide antibiotic exhibits broad-spectrum activity against Gram-negative and Gram-positive bacteria by interfering with bacterial protein synthesis, is unaffected by common resistance mechanisms and shows no toxicity towards human cells.
go.nature.com
March 26, 2025 at 5:40 PM
Nature research paper: A broad-spectrum lasso peptide antibiotic targeting the bacterial ribosome
https://go.nature.com/4iECjJz
https://go.nature.com/4iECjJz
Reposted by Kathrin Lang
Cells are filled with toxic stuff that damages healthy proteins. Is that garbage just left to rot on the curb? No way! Ubiquitin ligases have evolved to recognize chemical damage and clean it up! www.nature.com/articles/s41...
C-terminal amides mark proteins for degradation via SCF–FBXO31 - Nature
SCF–FBXO31 scans proteins for C-terminal amidation and marks them for subsequent proteasomal degradation.
www.nature.com
January 30, 2025 at 12:30 PM
Cells are filled with toxic stuff that damages healthy proteins. Is that garbage just left to rot on the curb? No way! Ubiquitin ligases have evolved to recognize chemical damage and clean it up! www.nature.com/articles/s41...
Reposted by Kathrin Lang
Elise and Tarah's work on engineering leucyl-tRNA synthetase to incorporate numerous ncAAs in mammalian cells now published! A bit of good news amidst these chaotic times onlinelibrary.wiley.com/doi/10.1002/...
Optimized Directed Evolution of E. coli leucyl‐tRNA Synthetase adds many Noncanonical Amino Acids into the Eukaryotic Genetic Code Including Ornithine and Nϵ‐Acetyl‐Methyllysine
The pyrrolysyl pair has dominated noncanonical amino acids (ncAAs) incorporation in eukaryotes, but additional engineerable pairs are needed to further expand this toolbox. Although E. coli leucyl-tR....
onlinelibrary.wiley.com
January 29, 2025 at 7:45 PM
Elise and Tarah's work on engineering leucyl-tRNA synthetase to incorporate numerous ncAAs in mammalian cells now published! A bit of good news amidst these chaotic times onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Kathrin Lang
📢 The Louis-Jeantet Foundation is happy to announce the 2025 #LouisJeantetPrizes, awarded to VEIT HORNUNG @v-hornung.bsky.social @genecenter-lmu.bsky.social and to GILLES LAURENT @ maxplanck.de
CONGRATULATIONS!!!
👉 jeantet.ch/en
👇 follow thread
CONGRATULATIONS!!!
👉 jeantet.ch/en
👇 follow thread
January 21, 2025 at 10:18 AM
📢 The Louis-Jeantet Foundation is happy to announce the 2025 #LouisJeantetPrizes, awarded to VEIT HORNUNG @v-hornung.bsky.social @genecenter-lmu.bsky.social and to GILLES LAURENT @ maxplanck.de
CONGRATULATIONS!!!
👉 jeantet.ch/en
👇 follow thread
CONGRATULATIONS!!!
👉 jeantet.ch/en
👇 follow thread
Reposted by Kathrin Lang
Overjoyed and honestly relieved to share a Chem Rev magnum opus from Brian Chen & Julia Li titled Synthetic Lipid Biology! In it, we organize efforts across numerous fields to create, manipulate, and analyze lipids and biomembranes. Check it out! doi.org/10.1021/acs.... #lipidtime #chemsky #chembio
January 14, 2025 at 1:49 PM
Overjoyed and honestly relieved to share a Chem Rev magnum opus from Brian Chen & Julia Li titled Synthetic Lipid Biology! In it, we organize efforts across numerous fields to create, manipulate, and analyze lipids and biomembranes. Check it out! doi.org/10.1021/acs.... #lipidtime #chemsky #chembio
Reposted by Kathrin Lang
A new tool FUSEP profiles the E2-specific ubiquitome and enables systematic studies of non-lysine ubiquitin conjugation, here used to identify the existence of tyrosine ubiquitination
www.nature.com/articles/s41...
www.nature.com/articles/s41...
E2–Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells - Nature Chemical Biology
Shen and Yin developed a technology named FUSEP to profile the E2-specific ubiquitome that makes use of a fusion between E2 and ubiquitin carrying a R74G point mutation, which leaves an LGGG remnant f...
www.nature.com
January 10, 2025 at 3:07 PM
A new tool FUSEP profiles the E2-specific ubiquitome and enables systematic studies of non-lysine ubiquitin conjugation, here used to identify the existence of tyrosine ubiquitination
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Kathrin Lang
Exciting paper by the groups of Ronald Micura and Alexandra Lusser in @nchembio.bsky.social. Covalent binders to structured RNA based on alkyl halides and alkyl mesylates show engagement of the preQ1 and Pepper aptamers in vitro and in cells. www.nature.com/articles/s41...
Engineering covalent small molecule–RNA complexes in living cells - Nature Chemical Biology
Small-molecule ligands have been developed that covalently attach to their cognate RNA aptamers in vitro and in living cells. This strategy opens up new avenues for RNA imaging applications (for examp...
www.nature.com
January 8, 2025 at 3:34 PM
Exciting paper by the groups of Ronald Micura and Alexandra Lusser in @nchembio.bsky.social. Covalent binders to structured RNA based on alkyl halides and alkyl mesylates show engagement of the preQ1 and Pepper aptamers in vitro and in cells. www.nature.com/articles/s41...
Reposted by Kathrin Lang
Die Fachgruppe Biochemie der @gdch.bsky.social wünscht ein frohes neues Jahr!
Wir hatten ein großartiges 2024 mit einer fantastischen Biochemie-Tagung. Wenn Sie in 2024 eine herausragende Doktorarbeit oder Veröffentlichung in Ihrer Gruppe hatten, nominieren Sie für unseren Förderpreis!
Wir hatten ein großartiges 2024 mit einer fantastischen Biochemie-Tagung. Wenn Sie in 2024 eine herausragende Doktorarbeit oder Veröffentlichung in Ihrer Gruppe hatten, nominieren Sie für unseren Förderpreis!
January 1, 2025 at 1:43 PM
Die Fachgruppe Biochemie der @gdch.bsky.social wünscht ein frohes neues Jahr!
Wir hatten ein großartiges 2024 mit einer fantastischen Biochemie-Tagung. Wenn Sie in 2024 eine herausragende Doktorarbeit oder Veröffentlichung in Ihrer Gruppe hatten, nominieren Sie für unseren Förderpreis!
Wir hatten ein großartiges 2024 mit einer fantastischen Biochemie-Tagung. Wenn Sie in 2024 eine herausragende Doktorarbeit oder Veröffentlichung in Ihrer Gruppe hatten, nominieren Sie für unseren Förderpreis!
Reposted by Kathrin Lang
Efficient circular RNA synthesis for potent rolling circle translation. Congratulations to Yifei and the team in Venki's lab at @mrclmb.bsky.social!
www.nature.com/articles/s41...
#RNA #RNABiology #RNAsky #Translation #mRNA
www.nature.com/articles/s41...
#RNA #RNABiology #RNAsky #Translation #mRNA
Efficient circular RNA synthesis for potent rolling circle translation - Nature Biomedical Engineering
Trans-splicing-based methods that are independent of bacterial sequences and that allow the incorporation of RNA modifications enable the reliable synthesis of circular RNAs and enhance the efficiency...
www.nature.com
December 18, 2024 at 1:01 AM
Efficient circular RNA synthesis for potent rolling circle translation. Congratulations to Yifei and the team in Venki's lab at @mrclmb.bsky.social!
www.nature.com/articles/s41...
#RNA #RNABiology #RNAsky #Translation #mRNA
www.nature.com/articles/s41...
#RNA #RNABiology #RNAsky #Translation #mRNA
Reposted by Kathrin Lang
WOW!!! Beautiful new Nature paper from Burkart Lab UCSD reveals how non-ribosomal peptide natural product synthetases work together.
They use tetrazine click chemistry to capture these huge protein complexes mid-reaction. 3 different snapshots show different states.
www.nature.com/articles/s41...
They use tetrazine click chemistry to capture these huge protein complexes mid-reaction. 3 different snapshots show different states.
www.nature.com/articles/s41...
December 14, 2024 at 3:47 AM
WOW!!! Beautiful new Nature paper from Burkart Lab UCSD reveals how non-ribosomal peptide natural product synthetases work together.
They use tetrazine click chemistry to capture these huge protein complexes mid-reaction. 3 different snapshots show different states.
www.nature.com/articles/s41...
They use tetrazine click chemistry to capture these huge protein complexes mid-reaction. 3 different snapshots show different states.
www.nature.com/articles/s41...
Reposted by Kathrin Lang
Curious what we do in my new lab at D-CHAB @ethzurich.bsky.social 🔬⚗️? Save the date for my inaugural lecture on April 3, 2025 and if you can’t wait to know more check out the news article and the associated video. chab.ethz.ch/en/news-and-...
December 5, 2024 at 12:05 PM
Curious what we do in my new lab at D-CHAB @ethzurich.bsky.social 🔬⚗️? Save the date for my inaugural lecture on April 3, 2025 and if you can’t wait to know more check out the news article and the associated video. chab.ethz.ch/en/news-and-...
Reposted by Kathrin Lang
Happy to see my Medicinal Chemistry and Chemical Biology Highlight out in the current CHIMIA and online. Thank you to @klanglab.bsky.social for the invitation!
www.chimia.ch/chimia/artic...
www.chimia.ch/chimia/artic...
A New Era for Peptide Therapeutics: Innovations, Challenges, and Future Directions: Medicinal Chemistry and Chemical Biology Highlights
| CHIMIA
www.chimia.ch
November 29, 2024 at 12:39 PM
Happy to see my Medicinal Chemistry and Chemical Biology Highlight out in the current CHIMIA and online. Thank you to @klanglab.bsky.social for the invitation!
www.chimia.ch/chimia/artic...
www.chimia.ch/chimia/artic...
Reposted by Kathrin Lang
First paper of our lab today @natureportfolio.bsky.social by Nussi!
We provide a real-time movie on how #transcription and #translation cooperate using #single-molecule FM: we find long-range #ribosome/ RNAP communication mediated by #RNA looping!
@embl.org #RNAbiology #RNASky
t.co/a24Xxcxdo0
We provide a real-time movie on how #transcription and #translation cooperate using #single-molecule FM: we find long-range #ribosome/ RNAP communication mediated by #RNA looping!
@embl.org #RNAbiology #RNASky
t.co/a24Xxcxdo0
December 4, 2024 at 8:53 PM
First paper of our lab today @natureportfolio.bsky.social by Nussi!
We provide a real-time movie on how #transcription and #translation cooperate using #single-molecule FM: we find long-range #ribosome/ RNAP communication mediated by #RNA looping!
@embl.org #RNAbiology #RNASky
t.co/a24Xxcxdo0
We provide a real-time movie on how #transcription and #translation cooperate using #single-molecule FM: we find long-range #ribosome/ RNAP communication mediated by #RNA looping!
@embl.org #RNAbiology #RNASky
t.co/a24Xxcxdo0
Reposted by Kathrin Lang
Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from gene expression and real-time fluorescence to modulation..
December 4, 2024 at 4:05 PM
Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from gene expression and real-time fluorescence to modulation..
Reposted by Kathrin Lang
I’m thrilled to share a collaborative story from @Mendell_lab and Jan Erzberger labs, led by postdoc extraordinaire Xiaoqiang Zhu. We showed that in addition to their canonical decoding function, tRNAs play a key role in regulating mRNA stability during translation!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes
The CCR4-NOT complex is a major regulator of eukaryotic messenger RNA (mRNA) stability. Slow decoding during translation promotes association of CCR4-NOT with ribosomes, accelerating mRNA degradation....
www.science.org
November 22, 2024 at 6:09 PM
I’m thrilled to share a collaborative story from @Mendell_lab and Jan Erzberger labs, led by postdoc extraordinaire Xiaoqiang Zhu. We showed that in addition to their canonical decoding function, tRNAs play a key role in regulating mRNA stability during translation!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...