Joachim Goedhart
@joachimgoedhart.bsky.social
Scientist + Teacher UvA/Amsterdam | Cells | Molecules | Microscopy | Fluorescent Proteins | Biosensors | Lifetime imaging | Open Science | dataViz | R | web apps
Homepage: https://joachimgoedhart.github.io/
DataViz Apps: https://huygens.science.uva.nl
Homepage: https://joachimgoedhart.github.io/
DataViz Apps: https://huygens.science.uva.nl
Pinned
There are now 24 step-by-step guides, explaining how to create these ⬇️ data visualizations with R/ggplot2 - I hope this is a useful resource. Find them all here: joachimgoedhart.github.io/DataViz-prot...
Reposted by Joachim Goedhart
We only EXCLUSIVELY use @reviewcommons.org for our papers. There is no point of doing otherwise. It is soooo much better.
November 27, 2025 at 8:37 PM
We only EXCLUSIVELY use @reviewcommons.org for our papers. There is no point of doing otherwise. It is soooo much better.
Reposted by Joachim Goedhart
New paper from the @rouxlab.bsky.social on Nature Communications! We study how membrane tension is spatially organized in cells. Using the mechanosensitive probe Flipper-TR to visualize tension across the plasma membrane of adherent cells and to dissect the conditions needed for a gradient to happen
November 27, 2025 at 2:14 PM
New paper from the @rouxlab.bsky.social on Nature Communications! We study how membrane tension is spatially organized in cells. Using the mechanosensitive probe Flipper-TR to visualize tension across the plasma membrane of adherent cells and to dissect the conditions needed for a gradient to happen
Reposted by Joachim Goedhart
Wuhuuu, preprints using the #RxivMaker engine are popping 🍾
Also, the PDF or our preprint was created with Rχiv-Maker
@henriqueslab.bsky.social
rxiv-maker.henriqueslab.org
Paper: arxiv.org/html/2508.00...
@henriqueslab.bsky.social
rxiv-maker.henriqueslab.org
Paper: arxiv.org/html/2508.00...
November 27, 2025 at 8:54 AM
Wuhuuu, preprints using the #RxivMaker engine are popping 🍾
Reposted by Joachim Goedhart
Interested in Zebrafish brain imaging? We released a 3D printed mold that's is super helpful for us. Check it out
Customizable FDM-based zebrafish embryo mold for live imaging
www.biorxiv.org/content/10.1...
Work by Marcela and Jaakko in the lab
Customizable FDM-based zebrafish embryo mold for live imaging
www.biorxiv.org/content/10.1...
Work by Marcela and Jaakko in the lab
November 27, 2025 at 6:53 AM
Interested in Zebrafish brain imaging? We released a 3D printed mold that's is super helpful for us. Check it out
Customizable FDM-based zebrafish embryo mold for live imaging
www.biorxiv.org/content/10.1...
Work by Marcela and Jaakko in the lab
Customizable FDM-based zebrafish embryo mold for live imaging
www.biorxiv.org/content/10.1...
Work by Marcela and Jaakko in the lab
Reposted by Joachim Goedhart
So excited to share this as a new junior PI:
My brand-new lab website! 🎉🪰🌀
www.bischofflab.com
Please pass it on to young, motivated researchers looking for PhD positions 😊
And for the #FluorescenceFriday community: don’t miss the SciArt Gallery!
#CellBio #DevBio #PhDjob #PhDposition #Science
My brand-new lab website! 🎉🪰🌀
www.bischofflab.com
Please pass it on to young, motivated researchers looking for PhD positions 😊
And for the #FluorescenceFriday community: don’t miss the SciArt Gallery!
#CellBio #DevBio #PhDjob #PhDposition #Science
November 27, 2025 at 8:26 AM
So excited to share this as a new junior PI:
My brand-new lab website! 🎉🪰🌀
www.bischofflab.com
Please pass it on to young, motivated researchers looking for PhD positions 😊
And for the #FluorescenceFriday community: don’t miss the SciArt Gallery!
#CellBio #DevBio #PhDjob #PhDposition #Science
My brand-new lab website! 🎉🪰🌀
www.bischofflab.com
Please pass it on to young, motivated researchers looking for PhD positions 😊
And for the #FluorescenceFriday community: don’t miss the SciArt Gallery!
#CellBio #DevBio #PhDjob #PhDposition #Science
Reposted by Joachim Goedhart
Excited to share our team’s work on improving FLIM metabolic imaging. NC-PCA selectively removes shot noise without filters or training. The result? Clearer phasors, higher accuracy, & better biological insights.
www.nature.com/articles/s41...
@springernature.com @academic-chatter.bsky.social 💡🧪
www.nature.com/articles/s41...
@springernature.com @academic-chatter.bsky.social 💡🧪
Denoising of fluorescence lifetime imaging data via principal component analysis - Scientific Reports
Scientific Reports - Denoising of fluorescence lifetime imaging data via principal component analysis
www.nature.com
November 26, 2025 at 8:09 PM
Excited to share our team’s work on improving FLIM metabolic imaging. NC-PCA selectively removes shot noise without filters or training. The result? Clearer phasors, higher accuracy, & better biological insights.
www.nature.com/articles/s41...
@springernature.com @academic-chatter.bsky.social 💡🧪
www.nature.com/articles/s41...
@springernature.com @academic-chatter.bsky.social 💡🧪
Reposted by Joachim Goedhart
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
#protistsonsky 🧵
November 25, 2025 at 8:41 PM
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
#protistsonsky 🧵
Reposted by Joachim Goedhart
For some research studies the optimal sample size should be estimated at 0
November 25, 2025 at 10:51 AM
For some research studies the optimal sample size should be estimated at 0
Redesign of Calmodulin for Genetically Encoded Zn2+ Sensing with High Dynamic Range
pubs.acs.org/doi/full/10....
pubs.acs.org/doi/full/10....
Redesign of Calmodulin for Genetically Encoded Zn2+ Sensing with High Dynamic Range
The development of genetically encoded Zn2+ probes (GEZPs) has been hindered by the limited signal transduction efficiency of natural zinc-binding domains, but the encoded Ca2+ indicators utilize the ...
pubs.acs.org
November 25, 2025 at 8:35 AM
Redesign of Calmodulin for Genetically Encoded Zn2+ Sensing with High Dynamic Range
pubs.acs.org/doi/full/10....
pubs.acs.org/doi/full/10....
In our new #Preprint, we pilot a revolutionary new idea: no supplemental data (it has 21 figures, though):
Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR
www.biorxiv.org/content/10.1...
Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR
www.biorxiv.org/content/10.1...
Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR
We have previously shown that the calcium sensing receptor (CaSR) activates different G proteins and second messengers in single cells, and that GPCR and G protein-mediated ERK activity can be highly ...
www.biorxiv.org
November 24, 2025 at 8:29 AM
In our new #Preprint, we pilot a revolutionary new idea: no supplemental data (it has 21 figures, though):
Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR
www.biorxiv.org/content/10.1...
Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR
www.biorxiv.org/content/10.1...
Reposted by Joachim Goedhart
{ripper} dissects your #Quarto documents, extracting code blocks by language and leaving executable scripts behind.
Supports #rstats, #python, #julia, and 13 other victims.
For those who never stopped celebrating Halloween.
📚 quarto.thecoatlessprofessor.com/ripper/
💻 github.com/coatless-qua...
Supports #rstats, #python, #julia, and 13 other victims.
For those who never stopped celebrating Halloween.
📚 quarto.thecoatlessprofessor.com/ripper/
💻 github.com/coatless-qua...
November 22, 2025 at 10:57 PM
{ripper} dissects your #Quarto documents, extracting code blocks by language and leaving executable scripts behind.
Supports #rstats, #python, #julia, and 13 other victims.
For those who never stopped celebrating Halloween.
📚 quarto.thecoatlessprofessor.com/ripper/
💻 github.com/coatless-qua...
Supports #rstats, #python, #julia, and 13 other victims.
For those who never stopped celebrating Halloween.
📚 quarto.thecoatlessprofessor.com/ripper/
💻 github.com/coatless-qua...
Revealing acute consequences of rapid degradation of synaptic fusion proteins at individual synapses using Auxin-Inducible Degron 2 technology
www.nature.com/articles/s42...
www.nature.com/articles/s42...
November 22, 2025 at 7:59 PM
Revealing acute consequences of rapid degradation of synaptic fusion proteins at individual synapses using Auxin-Inducible Degron 2 technology
www.nature.com/articles/s42...
www.nature.com/articles/s42...
Reposted by Joachim Goedhart
Life actually: over taaie systemen en bonte details.
Mijn oratie van 20 november 2025 komt binnenkort nog beschikbaar als PDF maar is nu al terug te kijken via tinyurl.com/OratieRenee
Mijn oratie van 20 november 2025 komt binnenkort nog beschikbaar als PDF maar is nu al terug te kijken via tinyurl.com/OratieRenee
November 21, 2025 at 7:55 PM
Life actually: over taaie systemen en bonte details.
Mijn oratie van 20 november 2025 komt binnenkort nog beschikbaar als PDF maar is nu al terug te kijken via tinyurl.com/OratieRenee
Mijn oratie van 20 november 2025 komt binnenkort nog beschikbaar als PDF maar is nu al terug te kijken via tinyurl.com/OratieRenee
Reposted by Joachim Goedhart
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
November 20, 2025 at 11:42 AM
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
Reposted by Joachim Goedhart
Soliciting recommendations for 3D printers and lab supply blue prints. Please share and RT.
November 21, 2025 at 2:24 AM
Soliciting recommendations for 3D printers and lab supply blue prints. Please share and RT.
Reposted by Joachim Goedhart
quick test with SAM3 - impressed with how well it seems to track long protrusions? prompted for "cell", does not find anything when looking for "fibroblast" or "nucleus"
November 19, 2025 at 8:15 PM
quick test with SAM3 - impressed with how well it seems to track long protrusions? prompted for "cell", does not find anything when looking for "fibroblast" or "nucleus"
Reposted by Joachim Goedhart
Check out this tick I collected ;-)
And by collected, I mean pulled off my pant leg after a walk in the woods
And by collected, I mean pulled off my pant leg after a walk in the woods
November 19, 2025 at 8:29 PM
Check out this tick I collected ;-)
And by collected, I mean pulled off my pant leg after a walk in the woods
And by collected, I mean pulled off my pant leg after a walk in the woods
Uploading >30 files to change our preprint into a VOR for @elife.bsky.social
What could possibly go wrong?
What could possibly go wrong?
November 19, 2025 at 1:58 PM
Uploading >30 files to change our preprint into a VOR for @elife.bsky.social
What could possibly go wrong?
What could possibly go wrong?
The annual course “Basics in Fluorescence Microscopy”, will be organised by the van Leeuwenhoek Centre for Advanced Microscopy from January 27th - 30th, 2026.
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Basics in fluorescence microscopy – Microscopycourse
www.microscopycourse.nl
November 19, 2025 at 1:48 PM
The annual course “Basics in Fluorescence Microscopy”, will be organised by the van Leeuwenhoek Centre for Advanced Microscopy from January 27th - 30th, 2026.
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Reposted by Joachim Goedhart
Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
November 19, 2025 at 6:27 AM
Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
Reposted by Joachim Goedhart
Major new direction in the lab: hacking human cell biology with pathogen effectors (eORFs) - amazing collaboration with @miketilapia.bsky.social lab. Huge congrats to first authors Tomas & He & all co-authors! Check out the pre-print on @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...
www.biorxiv.org
November 18, 2025 at 2:51 PM
Major new direction in the lab: hacking human cell biology with pathogen effectors (eORFs) - amazing collaboration with @miketilapia.bsky.social lab. Huge congrats to first authors Tomas & He & all co-authors! Check out the pre-print on @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...
Reposted by Joachim Goedhart
Reposted by Joachim Goedhart
Reposted by Joachim Goedhart
🔗 to paper: www.sciencedirect.com/science/arti...
When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.
November 14, 2025 at 8:42 PM
🔗 to paper: www.sciencedirect.com/science/arti...
Reposted by Joachim Goedhart
When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.
November 14, 2025 at 8:34 PM
When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.