Colin Conine
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colinconine.bsky.social
Colin Conine
@colinconine.bsky.social
640 followers 460 following 30 posts
Small RNA biologist at UPenn & CHOP
www.coninelab.com
Interested in small RNAs, germlines, inheritance, sports, and Shiba Inu’s
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colinconine.bsky.social
Colin Conine @colinconine.bsky.social · Apr 16
The first C. elegans paper from lab! We find that, similar to mammals, tRNA-fragments (or the new name in the field tDRs) accumulate in worm sperm and can transmit non-genetically inherited phenotypes to offspring. We also find an RNase that regulates their processing, showing that length matters
biorxivpreprint.bsky.social bioRxivpreprint @biorxivpreprint.bsky.social · Apr 16
tRNA-derived RNA processing in sperm transmits non-genetically inherited phenotypes to offspring in C. elegans https://www.biorxiv.org/content/10.1101/2025.04.14.648817v1
colinconine.bsky.social
Check out the spotlight @cambupenn.bsky.social student Lexie Schneider and I wrote about research demonstrating that Dad’s that exercise have sons that are more physically fit which is inherited by regulating miRNAs in sperm
November 21, 2025 at 1:33 PM
Reposted by Colin Conine
fusionconf.bsky.social
Pleased to share the programme for #GenReg26 is now live!

Head to the website to check out the sessions & speakers shaping our second edition➡️ bit.ly/3LGC4Cg

If you haven’t registered yet, now’s the time - final deadlines are fast approaching!⌛️
November 13, 2025 at 10:35 AM
Reposted by Colin Conine
biorxivpreprint.bsky.social
Diverse expression and modification of tRNAs and tRNA-derived RNAs in 19 mouse tissues https://www.biorxiv.org/content/10.1101/2025.11.11.685198v1
November 13, 2025 at 2:33 AM
Reposted by Colin Conine
steeltownworms.bsky.social
I wrote this insight for eLife. A friend said, "Why would you want to step on that rake?". Well, I guess that's just who I am. Enjoy. elifesciences.org/articles/109...
Transgenerational Epigenetic Inheritance: Twists and turns in the story of learned avoidance
Evidence that learned avoidance of a pathogenic bacterium can be transmitted to future generations in C. elegans is growing.
elifesciences.org
November 11, 2025 at 7:37 PM
Reposted by Colin Conine
lucksmith.bsky.social
new Lai lab paper @natsmb.nature.com! Dicer is specifically mutated in cancer, but we don't fully understand its molecular/reg impacts. with @danweihuangfu.bsky.social, we characterized the first knockin Dicer hotspot in hESCs, and found unexpected defects in miRNA biogenesis! 🧬 1/4

rdcu.be/eOc0q
Human DICER1 hotspot mutation induces both loss and gain of miRNA function
Nature Structural & Molecular Biology - Jee et al. study a cancer hotspot allele of DICER1 that disrupts RNaseIIIb activity. Beyond ablating 5p hairpin cleavage, 3p passenger strands are...
rdcu.be
November 10, 2025 at 4:43 PM
Reposted by Colin Conine
crisprflydesign.bsky.social
🤯This is wow! Molecular mechanism of mRNA export. by @juliusbrennecke.bsky.social @plaschkalab.bsky.social

www.nature.com/articles/s41...
Illustration of the molecular mechanism controlling mRNA export.
November 6, 2025 at 5:20 PM
Reposted by Colin Conine
bartellab.bsky.social
Check out related work by the Mendell lab (@mendell-lab.bsky.social) and the Xie lab: www.biorxiv.org/content/10.1..., www.biorxiv.org/content/10.1... (3/3)
Plagl1 and Lrrc58 control mammalian body size by triggering target-directed microRNA degradation of miR-322 and miR-503
Precise control of microRNA (miRNA) expression is critical during development. An important mechanism of miRNA regulation is target-directed microRNA degradation (TDMD), a pathway in which the binding...
www.biorxiv.org
November 7, 2025 at 6:33 PM
Reposted by Colin Conine
bartellab.bsky.social
Check out the latest work from our lab, led by Daniel Lin and Lara Elcavage: www.biorxiv.org/content/10.1... (1/3)
mRNA 3′ UTRs direct microRNA degradation to participate in imprinted gene networks and regulate growth
MicroRNAs direct downregulation of target mRNAs. Sometimes, however, this regulatory paradigm inverts, and a target RNA triggers the degradation of a microRNA. This target-directed microRNA degradatio...
www.biorxiv.org
November 7, 2025 at 6:33 PM
Reposted by Colin Conine
patrickphillips.bsky.social
Make males live more than 400% longer and prevent sexual disfunction late in life?! We're down with it. Our latest preprint: Disruption of the insulin signaling pathway in C. elegans dramatically increases male longevity and enhances reproductive health late in life. www.biorxiv.org/content/10.1...
November 6, 2025 at 11:14 PM
Reposted by Colin Conine
kurianlab.bsky.social
www.biorxiv.org/content/10.1...
Pseudouridine selects RNAs for extracellular transport
RNAs move through the extracellular space to transmit information between cells, including mammalian neurons, yet how specific RNAs are channeled into these extracellular routes is unknown. Using geno...
www.biorxiv.org
November 5, 2025 at 2:57 PM
Reposted by Colin Conine
genesdev.bsky.social
🎥 G&D Tapes 🎥

G&D author, Haoming Yu tells us about their new study in #genesdev that uses epigenetic editing to probe the role of H3K4me3 at intergenic regulatory elements.

Learn more here:
➡️ https://genesdev.cshlp.org/content/39/21-22/1290.full

Bluma Lesch
@haomingyu.bsky.social

November 4, 2025 at 7:02 PM
Reposted by Colin Conine
raflynn5.bsky.social
And another interesting paper from Bonasio lab @upenn.edu www.biorxiv.org/content/10.1... "Pseudouridine selects RNAs for extracellular transport" noting among other things "..that MYL6 is a pU-binding protein required for secretion of RNAs." notable MYL6 is found in the Kossiakoff work as a csRBP!
Pseudouridine selects RNAs for extracellular transport
RNAs move through the extracellular space to transmit information between cells, including mammalian neurons, yet how specific RNAs are channeled into these extracellular routes is unknown. Using geno...
www.biorxiv.org
November 2, 2025 at 7:44 PM
Reposted by Colin Conine
distefanolab.bsky.social
1/ Excited to share our new study with @brumbaugh-lab.bsky.social, out in @natbiotech.nature.com! P-bodies selectively sequester RNAs encoding cell fate regulators, often from the preceding developmental stage. Releasing these RNAs can drive changes in cell identity. 🧵 www.nature.com/articles/s41...
Selective RNA sequestration in biomolecular condensates directs cell fate transitions - Nature Biotechnology
Stem cell differentiation is controlled by manipulating RNA condensates.
www.nature.com
October 28, 2025 at 4:02 PM
Reposted by Colin Conine
biorxivpreprint.bsky.social
Argonautes and small RNAs associated with nematode programmed DNA elimination https://www.biorxiv.org/content/10.1101/2025.10.23.684123v1
October 24, 2025 at 2:19 AM
Reposted by Colin Conine
juliecocquet.bsky.social
Big thanks to all the speakers for their excellent talks: Adelheid Soubry @jamiehackett.bsky.social @nkotaja.bsky.social @romainbarres.bsky.social
@epitep.bsky.social
Anais Vitorino-Carvalho, kaylee Holleman, amrita Raja-ravi-shankar and Blair Schneider from the @colinconine.bsky.social lab
October 20, 2025 at 4:08 PM
Reposted by Colin Conine
natbiotech.nature.com
microRNAs blaze into the clinic www.nature.com/articles/s41...
microRNAs blaze into the clinic - Nature Biotechnology
After decades-long struggles with microRNA-based therapeutics, Regulus hits paydirt in the clinic and in the marketplace. Is it the beginning of a trend or a one-off?
www.nature.com
October 16, 2025 at 6:13 PM
Reposted by Colin Conine
averydb.bsky.social
Did you know that the genes for critical, ancient components of protein synthesis machinery - tRNAs - are bombarded with mutations, leading to remarkable allelic diversity that likely has functional consequences? I didn't until we did this project!
Preprint: www.biorxiv.org/content/10.1...
(1/2)
Nuclear-encoded tRNA genes harbor substantial allelic diversity in three nematode species
Cytosolic transfer RNAs, which are encoded as hundreds of genes in eukaryotic nuclear genomes, experience exceptionally high rates of mutation and have been hypothesized to carry significant mutationa...
www.biorxiv.org
October 16, 2025 at 3:42 PM
Reposted by Colin Conine
science.org
Male mice that exercise can pass their newly gained fitness on to male offspring.

If the same holds true in humans, researchers say, fathers could help improve the health of any future children by staying in shape themselves. https://scim.ag/47igpZm
Well-exercised male mice appear to pass fitness to their male offspring
Surprising epigenetic effect relies on snippets of RNA packaged within sperm
scim.ag
October 13, 2025 at 1:30 PM
Reposted by Colin Conine
obog.bsky.social
www.embopress.org/doi/full/10....
A single-cell multiomics roadmap of zebrafish spermatogenesis reveals regulatory principles of male germline formation | Molecular Systems Biology
imageimageA multiomics study of zebrafish spermatogenesis integrating scRNA-seq, scATAC-seq, and base-resolution DNA methylomes defines the chromatin and transcriptional states of germ cells, revealing the regulatory dynamics of this continuous ...
www.embopress.org
October 14, 2025 at 6:32 PM
Reposted by Colin Conine
natrevgenet.nature.com
The somatic DNA methylation landscape
Figure from our #Review by Zachary D. Smith, Sara Hetzel & Alexander Meissner: DNA methylation in mammalian development and disease go.nature.com/3DlswZn
Free to read here: rdcu.be/dQEND
Magnified view of partially methylated domains or highly methylated domains over CpG islands and DNA methylation valleys (DMVs)
May 28, 2025 at 11:25 AM
Reposted by Colin Conine
rti-umasschan.bsky.social
Hot off the press from the RTI: mRNA initiation and termination are spatially coordinated
buff.ly/vwSuPWC #RNA #RNATherapeutics
mRNA initiation and termination are spatially coordinated - PubMed
Transcriptional initiation and termination decisions drive messenger RNA (mRNA) isoform diversity but the relationship between them remains poorly understood. By systematically profiling joint usage of...
buff.ly
October 10, 2025 at 5:02 PM
Reposted by Colin Conine
genesdev.bsky.social
RESEARCH PAPER: A noncanonical Pol III-dependent, Microprocessor-independent biogenesis pathway generates a germline-enriched miRNA family
By Sakhawala et al. and Katherine McJunkin

Learn more here:
➡️ https://genesdev.cshlp.org/content/39/19-20/1198.abstract
October 10, 2025 at 3:00 PM
Reposted by Colin Conine
mattneville.bsky.social
Now published! Our paper on:
(1) Accurate sequencing of sperm at scale
(2) Positive selection of spermatogenesis driver mutations across the exome
(3) Offspring disease risks from male reproductive aging
[1/n]
www.nature.com/articles/s41...
Sperm sequencing reveals extensive positive selection in the male germline - Nature
A combination of whole-genome NanoSeq with deep whole-exome and targeted NanoSeq is used to accurately characterize mutation rates and genes under positive selection in sperm cells.
www.nature.com
October 8, 2025 at 3:51 PM
Reposted by Colin Conine
vseplyarskiy.bsky.social
Our paper on clonal expansions in Sperm is out in Nature www.nature.com/articles/s41...
If you are interested in working at an intersection of Mendelian genomics/Population genetics/Clonal expansions +Cancer genetics/ and of course mutagenesis, please rich out about postdoc in my lab
October 8, 2025 at 4:52 PM
Reposted by Colin Conine
natbiotech.nature.com
Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells go.nature.com/42wdD05
Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells - Nature Biotechnology
Haploid embryonic stem cells for cattle and sheep are produced, opening new reproductive possibilities.
go.nature.com
October 7, 2025 at 2:28 PM