Author | Lightnews

Reposted by Max Clabbers

Mike Martynowycz @sciencemick.bsky.social · Jul 5

Seed to sub-Å!
We just solved crambin at 0.85Å with #MicroED straight from spontaneous nanocrystals, phased ab-initio from a 5-res helix. Video shows diffraction ➜ ugly start map ➜ beautiful spheres. Preprint: www.biorxiv.org/content/10.1...
#StructuralBiology #CryoEM

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Reposted by Max Clabbers

David A. Delgadillo @daverdude.bsky.social · Jun 3

There's been a rich connection between electron diffraction and the nature of the chemical bond over the years. Here, we've shown how you can leverage electron diffraction to discover and characterize novel natural products.
@caltechcce.bsky.social @uclacb.bsky.social
pubs.acs.org/doi/10.1021/...

Microcrystal Electron Diffraction-Guided Discovery of Fungal Metabolites

Nature remains a vast repository of complex and functional metabolites whose structural characterization continues to drive innovations in pharmaceuticals, agrochemicals, and materials science. The cr...

pubs.acs.org

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Reposted by Max Clabbers

Ken Inge @keninge.bsky.social · Jun 3

Carmine is a red pigment used since antiquity to color textiles, in paintings by Rembrandt and Van Gogh, and today as a food coloring agent (E120).

Very excited that @esvenssongrape.bsky.social finally reveals the crystal structure of carmine by #3DED!

pubs.acs.org/doi/10.1021/...

Brilliantly Red: The Structure of Carmine

Carmine is a red pigment made from dried cochineal, a scale insect that has been a source of brilliant scarlet reds in clothing and art for more than two millennia, with records dating back to 700 BC....

pubs.acs.org

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Reposted by Max Clabbers

William J Nicolas @wjnicol.bsky.social · Mar 27

Happy to share the latest work from the Gonen lab! A collective effort to solve the structure of the lens membrane protein central to keeping our lenses transparent!
Fluorescent targeted FIB milling, LCP and microED made this possible!
www.nature.com/articles/s41...

Structure of the lens MP20 mediated adhesive junction - Nature Communications

Human lens clarity and function depends on well-organized cell junctions. Here, the authors used MicroED to reveal the 3.5 Å structure of MP20, showing that MP20 tetramers form adhesive junctions esse...

www.nature.com

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Reposted by Max Clabbers

Ricardo D. Righetto @lifeonthewedge.bsky.social · Mar 20

WE GOT THE COVER!!!

Science Magazine @science.org · Mar 20

In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion.

Learn more in this week's issue: scim.ag/3FA3Ygq

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Max Clabbers @maxclabbers.bsky.social · Mar 12

Our latest paper is out!

We combine direct electron detection and energy filtering to boost the SNR of our MicroED data

This work was done at HHMI and UCLA with @sciencemick.bsky.social who now runs his own lab at UB-HWI, Johan Hattne, and Tamir Gonen

www.nature.com/articles/s41...

Energy filtering enables macromolecular MicroED data at sub-atomic resolution - Nature Communications

High-resolution data are crucial for accurate structural modeling. Here, authors enhance MicroED data quality using energy filtering, achieving sub-atomic resolution protein data and uncovering diffus...

www.nature.com

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Reposted by Max Clabbers

Nature Synthesis @natsynth.nature.com · Jan 13

Now online & open access:

Article by Yi Luo, Hao Xu, Yue Han, Xiaodong Zou, Peng Wu & co-workers

Atomic-scale insights into topotactic transformations in an extra-large-pore zeolite using time-resolved 3D electron diffraction

www.nature.com/articles/s44...
#chemsky

Atomic-scale insights into topotactic transformations in an extra-large-pore zeolite using time-resolved 3D electron diffraction - Nature Synthesis

Time-resolved three-dimensional electron diffraction captures reaction intermediates at the atomic scale during the topotactic transformations of a zeolite. It shows the structures of the two extra-la...

www.nature.com

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Reposted by Max Clabbers

Stockholm University @stockholm-uni.bsky.social · Feb 13

Wallenberg Scholar Xiaodong Zou is developing analytical methods that image small molecules in three dimensions and in greater detail than ever before. This opens the way for better drugs and new fundamental discoveries in chemistry. @kawresearch.bsky.social www.su.se/english/news...

Three-dimensional images enhance our molecular understanding of nature - Stockholm University

Three-dimensional images enhance our molecular understanding of nature Xiaodong Zou has long dreamed of being able to take pictures of the tiniest constituents of nature, such as aromatic substances –...

www.su.se

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Reposted by Max Clabbers

Sven Klumpe @svenklumpe.bsky.social · Mar 5

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

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Reposted by Max Clabbers

Mike Martynowycz @sciencemick.bsky.social · Mar 6

Our work in Nature Comms demonstrates how energy filtering, plasma FIB, and direct electron detectors in counting mode can push MicroED to sub-atomic resolution!

Proud to have done this work back at UCLA. Hoping to bring similar capabilities to Buffalo.

📄 Read the paper: doi.org/10.1038/s414...

Energy filtering enables macromolecular MicroED data at sub-atomic resolution - Nature Communications

High-resolution data are crucial for accurate structural modeling. Here, authors enhance MicroED data quality using energy filtering, achieving sub-atomic resolution protein data and uncovering diffus...

doi.org

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Reposted by Max Clabbers

Ricardo D. Righetto @lifeonthewedge.bsky.social · Feb 28

To all #TeamTomo #CryoET and #Chlamydomonas aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽‍💻

www.ebi.ac.uk/empiar/EMPIA...

A little thread about what's new... 🧵 1/n

Rare cellular events observed in the cryo-ET dataset. Slices through tomograms (left) with corresponding 3D segmentation (middle and right). Right panels show enlarged views of the boxed regions in the middle panels. A) Mitochondrion at the plasma membrane, tethered to microtubules by thin filaments of unknown identity. Segmented classes: nuclear envelope (light blue), nuclear pore complex (dark blue), 80S ribosomes (orange), mitochondrial membranes (dark red), ATP synthase (royal blue), plasma membranes (grey), microtubules (pink), filaments (yellow), endoplasmic reticulum (purple). Zoomed-in panel shows thin filaments running between microtubules and the mitochondrion at the plasma membrane. Note that this tomogram contains two closely appressed cells, and thus, two plasma membranes with cell walls between. B) Mitochondrial fission event with ER membrane interactions. Segmented classes: mitochondrial membranes (dark red), ATP synthase (royal blue), 80S ribosomes (orange), thylakoid membranes (green), PSII (bright yellow), cell wall (grey), ER (purple), fission site containing filamentous structures perpendicular to the mitochondrial long axis (pale yellow). C) Ciliary transition zone between basal body and axoneme, including assembling IFT train and stellate structure77. Segmented classes: microtubule doublets (light blue), central microtubule pair (light green), stellate structure (pale red), IFT train (yellow). D) Pyrenoid tubule extending from the thylakoids into the phase-separated Rubisco matrix of the pyrenoid. Minitubules originate from thylakoid membranes82. Segmented classes: thylakoid membranes (dark green), PSII (yellow), pyrenoid tubule (lime green), Rubisco (lavender blue), minitubules (orange). Scale bars in A-D: 100 nm. Related to Fig. 2C-H.

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Reposted by Max Clabbers

Ben Engel @cellarchlab.com · Jan 6

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍

Preprint📜: www.biorxiv.org/content/10.1...

A short thread🧵👇

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Reposted by Max Clabbers

Mike Martynowycz @sciencemick.bsky.social · Dec 9

Excited to share our new work! We shed light on the sociology of virus-induced condensates supporting reovirus assembly and replication. Visualized using #CryoET #CryoEM and #FIBmilling

www.nature.com/articles/s41...

Great collab w/ @xianxia13 @zhou_lab Xiaoyu Liu @gonenlab

Molecular sociology of virus-induced cellular condensates supporting reovirus assembly and replication - Nature Communications

Virus-induced cellular condensates are poorly understood. Using cryoET and cryoEM, the authors visualized the 3D “molecular sociology” of host-virus interactions and choreographed mammalian reovirus a...

www.nature.com

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Reposted by Max Clabbers

Mike Martynowycz @sciencemick.bsky.social · Dec 20

Super happy to share our latest work! A detailed protocol for #MicroED sample prep available @NatureProtocols! It covers #FIB, grid making, sample handling, #CryoFLM, and more! Hope it helps!

www.nature.com/articles/s41...

Comprehensive microcrystal electron diffraction sample preparation for cryo-EM - Nature Protocols

We present a protocol for preparing microcrystal samples for cryo-EM diffraction imaging, including room temperature solid-state small molecules and soluble and membrane protein crystals.

www.nature.com

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