Genome editing is a technology that enables targeted mutagenesis. Notably, site-directed nucleation (SDN)-1 genome editing, which does not involve the incorporation of foreign DNA sequences and can introduce the same mutations as naturally occurring mutations, is not subject to genetically modified organism (GMO) regulations in many countries if transgenes are segregated out. This makes it an attractive strategy for crop improvement. Multiple DNA double-strand breaks introduced via genome editing may lead to inversions or translocations. If these genomic alterations involve promoter regions, a promoter/enhancer replacement may occur, thereby altering target gene expression as desired. Because conventional SDN-1 genome editing primarily induces loss-of-function mutations, promoter/enhancer replacement by genome editing (PERGE) represents a new paradigm in genome editing for crop improvement.

Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...

Engineered DNA recombinases efficiently and specifically insert genetic cargos without the use of landing pads.

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The analysis of DNA sequence outcomes provides molecular insights into double-strand break (DSB) repair mechanisms. Using parallel in-pool profiling of Cas9-induced insertions and deletions (indels) w...

Directed evolution can rapidly generate genetic variants with new and enhanced properties, yet efficient platforms for performing such evolution directly in plant cells have been lacking. We developed...

Engineered prime editor systems with reduced occurrences of unwanted insertions or deletions during genome editing are developed.

Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. H...

Abstract. Targeted gene editing can be achieved using CRISPR–Cas9-assisted recombineering. However, high-efficiency editing requires careful optimization f

Profiling tolerated amino acid deletions in proteins can elucidate structure-function relationships, reconstruct intermediate stages in protein evolution, and be used to engineer minimized versions of...

Programmable DNA integration using CRISPR-associated transposons (CASTs) offers powerful capabilities for genome engineering. The single effector Cas12k CAST examples evolved from a fixed guide TnpB n...

WASHINGTON—Claiming the luminous potion would make the nation strong and vigorous, Health and Human Services Secretary Robert F. Kennedy Jr. issued an order Friday directing all Americans to drink a m...