Animalcules
@bacteriophile.bsky.social
Bacteria lover. Good, bad, or other wise. Beneficial, commensal, or pathogenic alike.
Authorized GMB (Genetically Modified Bacteria) scientist
Authorized GMB (Genetically Modified Bacteria) scientist
Pleased to share our recent article in PNAS - a collaboration with @jessicaandreani.bsky.social & Pablo Radicella, with important roles played by many members of each team.
A tripartite protein complex promotes DNA transport during natural transformation in Firmicutes www.pnas.org/doi/10.1073/...
A tripartite protein complex promotes DNA transport during natural transformation in Firmicutes www.pnas.org/doi/10.1073/...
A tripartite protein complex promotes DNA transport during natural transformation in Firmicutes | PNAS
Natural genetic transformation is a conserved mechanism of bacterial horizontal gene
transfer, which is directed entirely by the recipient cell and...
www.pnas.org
November 26, 2025 at 9:22 AM
Reposted by Animalcules
Accelerating science with AI
"By empowering researchers across all disciplines and institutions to accelerate science and engineering, AI could increase the productivity and impact of research, boost innovation, fuel economic growth, and improve people’s lives."
www.science.org/doi/10.1126/...
"By empowering researchers across all disciplines and institutions to accelerate science and engineering, AI could increase the productivity and impact of research, boost innovation, fuel economic growth, and improve people’s lives."
www.science.org/doi/10.1126/...
November 26, 2025 at 3:45 AM
Accelerating science with AI
"By empowering researchers across all disciplines and institutions to accelerate science and engineering, AI could increase the productivity and impact of research, boost innovation, fuel economic growth, and improve people’s lives."
www.science.org/doi/10.1126/...
"By empowering researchers across all disciplines and institutions to accelerate science and engineering, AI could increase the productivity and impact of research, boost innovation, fuel economic growth, and improve people’s lives."
www.science.org/doi/10.1126/...
ribosomologists (bacteria dept.) take note 👇
Evolutionary Flexibility of Ribosome Biogenesis in Bacteria | Molecular Biology and Evolution | Oxford Academic https://academic.oup.com/mbe/article/42/11/msaf288/8340222?login=false
November 25, 2025 at 11:06 AM
Reposted by Animalcules
High-throughput cultivation and isolation of environmental anaerobes using selectively permeable hydrogel capsules. academic.oup.com/ismecommun/a...
High-throughput cultivation and isolation of environmental anaerobes using selectively permeable hydrogel capsules
Abstract. Over the past two decades, metagenomics has greatly expanded our understanding of microbial phylogenetic and metabolic diversity. However, most m
academic.oup.com
November 25, 2025 at 8:56 AM
High-throughput cultivation and isolation of environmental anaerobes using selectively permeable hydrogel capsules. academic.oup.com/ismecommun/a...
Reposted by Animalcules
#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
November 24, 2025 at 4:57 PM
#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
Reposted by Animalcules
Arbitrium systems control lysis/lysogeny through the regulation of small antirepressor proteins www.biorxiv.org/content/10.1...
November 25, 2025 at 5:59 AM
Arbitrium systems control lysis/lysogeny through the regulation of small antirepressor proteins www.biorxiv.org/content/10.1...
Reposted by Animalcules
Sang Yup Lee’s research is at another level.
November 16, 2025 at 1:11 PM
Sang Yup Lee’s research is at another level.
Reposted by Animalcules
Molecular Microbiology | Microbiology Journal | Wiley Online Library onlinelibrary.wiley.com/doi/10.1111/...
#MicroSky
#MicroSky
Cyclic Di‐AMP Affects Cell Membrane Integrity of Streptococcus pneumoniae
When Streptococcus pneumoniae produces low amounts of the second messenger cyclic di-AMP (c-di-AMP), they do not survive in the presence of competence-stimulating peptide (CSP), which is used for tra...
onlinelibrary.wiley.com
November 24, 2025 at 8:02 PM
Molecular Microbiology | Microbiology Journal | Wiley Online Library onlinelibrary.wiley.com/doi/10.1111/...
#MicroSky
#MicroSky
Molecular Microbiology | Microbiology Journal | Wiley Online Library onlinelibrary.wiley.com/doi/10.1111/...
#MicroSky
#MicroSky
A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi
We developed a resource set and protocols that allow researchers to quickly alter or silence genes in Group B Streptococcus. Using flexible plasmids and a drug-inducible Cas12a system, the approach e....
onlinelibrary.wiley.com
November 24, 2025 at 7:59 PM
Molecular Microbiology | Microbiology Journal | Wiley Online Library onlinelibrary.wiley.com/doi/10.1111/...
#MicroSky
#MicroSky
Reposted by Animalcules
It's the season for being thankful - THANK A MICROBE returns! Here's the first of several student flyers appreciating the wonders of our microbial world!
November 23, 2025 at 11:31 PM
It's the season for being thankful - THANK A MICROBE returns! Here's the first of several student flyers appreciating the wonders of our microbial world!
Reposted by Animalcules
Reposted by Animalcules
Sharing this important article... for no reason whatsoever.
"We must also continue to deepen and refine our understanding of fundamental biological processes because these details frequently hold the keys to major advances in applied research."
elifesciences.org/articles/102...
"We must also continue to deepen and refine our understanding of fundamental biological processes because these details frequently hold the keys to major advances in applied research."
elifesciences.org/articles/102...
November 23, 2025 at 12:21 AM
Sharing this important article... for no reason whatsoever.
"We must also continue to deepen and refine our understanding of fundamental biological processes because these details frequently hold the keys to major advances in applied research."
elifesciences.org/articles/102...
"We must also continue to deepen and refine our understanding of fundamental biological processes because these details frequently hold the keys to major advances in applied research."
elifesciences.org/articles/102...
Reposted by Animalcules
Our latest T7SS study is now out in Science Advances!
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
A ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system
Cryo-EM structure reveals how a ubiquitin-like protein orchestrates assembly of a bacterial weapon system.
www.science.org
November 21, 2025 at 7:05 PM
Our latest T7SS study is now out in Science Advances!
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
Delighted to report that our group's passion project over the last 6+ years to make DNA cloning more accessible, efficient, and scalable using a software-assisted workflow called CloneCoordinate is now out in ACS Synbio!
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
CloneCoordinate: Open-Source Software for Collaborative DNA Construction
Custom DNA constructs have never been more common or important in the life sciences. Many researchers therefore devote substantial time and effort to molecular cloning, aided by abundant computer-aide...
pubs.acs.org
November 22, 2025 at 12:24 AM
Reposted by Animalcules
Hot off the press! Our latest paper led by @fernpizza.bsky.social, understanding how plasmids evolve inside cells. These small, self-replicating DNA circles live inside bacteria and carry antibiotic resistance genes, but also compete with one another to replicate. 1/
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Intracellular competition shapes plasmid population dynamics
From populations of multicellular organisms to selfish genetic elements, conflicts between levels of biological organization are central to evolution. Plasmids are extrachromosomal, self-replicating g...
www.science.org
November 20, 2025 at 9:42 PM
Hot off the press! Our latest paper led by @fernpizza.bsky.social, understanding how plasmids evolve inside cells. These small, self-replicating DNA circles live inside bacteria and carry antibiotic resistance genes, but also compete with one another to replicate. 1/
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Animalcules
🚨Preprint alert - this is a big one! We transfer the revolutionary power of TnSeq to bacteriophages.
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
November 20, 2025 at 8:40 PM
🚨Preprint alert - this is a big one! We transfer the revolutionary power of TnSeq to bacteriophages.
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
Reposted by Animalcules
New preprint! We measured temperature- and pH-induced aggregation for over 18,000 natural and de novo designed protein domains!
November 19, 2025 at 9:16 PM
New preprint! We measured temperature- and pH-induced aggregation for over 18,000 natural and de novo designed protein domains!