George Pobegalov
@thelastegor.bsky.social
PostDoc in Molodtsov Lab
The Francis Crick Institute, London
Optical tweezers, single-molecule fluorescence microscopy
SMC complexes, DNA repair, CRISPR
The Francis Crick Institute, London
Optical tweezers, single-molecule fluorescence microscopy
SMC complexes, DNA repair, CRISPR
Reposted by George Pobegalov
Glad to see this out after a rather interesting review/publication process!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The SMC Hinge is a Selective Gate for Obstacle Bypass - Nature Communications
SMC complexes are ring-shaped motors that fold DNA by extruding loops, but how they navigate large DNA obstacles is unclear. Here, Liu et al., show that SMC complexes bypass obstacles by threading obs...
www.nature.com
November 25, 2025 at 2:39 PM
Glad to see this out after a rather interesting review/publication process!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by George Pobegalov
We are recruiting a postdoctoral fellow to study mechanisms of DNA replication stress using single-molecule imaging tools. If you are excited about microscopy, replisome dynamics, and genome stability, we’d love to hear from you.
Application deadline: 24 December
www.crick.ac.uk/careers-stud...
Application deadline: 24 December
www.crick.ac.uk/careers-stud...
Postdoctoral Fellow - H Yardimci Lab
www.crick.ac.uk
November 25, 2025 at 11:55 AM
We are recruiting a postdoctoral fellow to study mechanisms of DNA replication stress using single-molecule imaging tools. If you are excited about microscopy, replisome dynamics, and genome stability, we’d love to hear from you.
Application deadline: 24 December
www.crick.ac.uk/careers-stud...
Application deadline: 24 December
www.crick.ac.uk/careers-stud...
Reposted by George Pobegalov
‼️ Excited to share our new paper out now in @science.org ‼️
We describe a new tetrameric RAD51 paralog complex – XRCC3-RAD51C-RAD51D-XRCC2 – which caps the end of RAD51 filaments.
Link: www.science.org/doi/epdf/10....
Thread ⬇️ (1/8)
We describe a new tetrameric RAD51 paralog complex – XRCC3-RAD51C-RAD51D-XRCC2 – which caps the end of RAD51 filaments.
Link: www.science.org/doi/epdf/10....
Thread ⬇️ (1/8)
November 7, 2025 at 10:15 AM
‼️ Excited to share our new paper out now in @science.org ‼️
We describe a new tetrameric RAD51 paralog complex – XRCC3-RAD51C-RAD51D-XRCC2 – which caps the end of RAD51 filaments.
Link: www.science.org/doi/epdf/10....
Thread ⬇️ (1/8)
We describe a new tetrameric RAD51 paralog complex – XRCC3-RAD51C-RAD51D-XRCC2 – which caps the end of RAD51 filaments.
Link: www.science.org/doi/epdf/10....
Thread ⬇️ (1/8)
Reposted by George Pobegalov
How do SMC complexes load onto DNA to get ready for loop extrusion?
@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸♂️🤸♂️🤸♂️.
Check out the new paper: www.cell.com/molecular-ce...
@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸♂️🤸♂️🤸♂️.
Check out the new paper: www.cell.com/molecular-ce...
October 9, 2025 at 2:43 PM
How do SMC complexes load onto DNA to get ready for loop extrusion?
@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸♂️🤸♂️🤸♂️.
Check out the new paper: www.cell.com/molecular-ce...
@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸♂️🤸♂️🤸♂️.
Check out the new paper: www.cell.com/molecular-ce...
Reposted by George Pobegalov
A diverse team of Crick researchers has come together to make some surprising new insights into how copies of DNA are held together by the ring-shaped protein complex cohesin during the replication process.
www.crick.ac.uk/news/2025-09...
www.crick.ac.uk/news/2025-09...
DNA’s double act: how genetic copies stick together during replication
Two leading DNA researchers have come together to confirm long-standing theories and uncover surprising new insights into how life’s code is copied and held together.
www.crick.ac.uk
September 16, 2025 at 3:11 PM
A diverse team of Crick researchers has come together to make some surprising new insights into how copies of DNA are held together by the ring-shaped protein complex cohesin during the replication process.
www.crick.ac.uk/news/2025-09...
www.crick.ac.uk/news/2025-09...
Reposted by George Pobegalov
Our Wadjet-II paper by @roisnehamelinf.bsky.social is out in polished form: www.cell.com/structure/fu...
With a great preview by @kevincorbett.bsky.social & @amardeeep.bsky.social highlighting the potential of Wadjets:
Wadjet—Keeping a watchful eye on circular DNA.
www.cell.com/structure/fu...
With a great preview by @kevincorbett.bsky.social & @amardeeep.bsky.social highlighting the potential of Wadjets:
Wadjet—Keeping a watchful eye on circular DNA.
www.cell.com/structure/fu...
September 5, 2025 at 6:29 AM
Our Wadjet-II paper by @roisnehamelinf.bsky.social is out in polished form: www.cell.com/structure/fu...
With a great preview by @kevincorbett.bsky.social & @amardeeep.bsky.social highlighting the potential of Wadjets:
Wadjet—Keeping a watchful eye on circular DNA.
www.cell.com/structure/fu...
With a great preview by @kevincorbett.bsky.social & @amardeeep.bsky.social highlighting the potential of Wadjets:
Wadjet—Keeping a watchful eye on circular DNA.
www.cell.com/structure/fu...
Reposted by George Pobegalov
Our paper is now out in Molecular Cell!
Check the thread in this former post:
www.cell.com/molecular-ce...
Check the thread in this former post:
www.cell.com/molecular-ce...
July 17, 2025 at 5:41 PM
Our paper is now out in Molecular Cell!
Check the thread in this former post:
www.cell.com/molecular-ce...
Check the thread in this former post:
www.cell.com/molecular-ce...
Reposted by George Pobegalov
I'm very pleased to share this work, which discovers a new material property of DNA—the bending friction— in essence how "sticky or viscous" DNA is as it bends & loops due to all the complex molecular interactions within itself & water
@imperialcollegeldn.bsky.social
@crick.ac.uk
@ucl.ac.uk
⚛️🧪
@imperialcollegeldn.bsky.social
@crick.ac.uk
@ucl.ac.uk
⚛️🧪
A new material constant of DNA is the major determinant of looping dynamics https://www.biorxiv.org/content/10.1101/2025.07.10.664086v1
July 16, 2025 at 11:56 PM
I'm very pleased to share this work, which discovers a new material property of DNA—the bending friction— in essence how "sticky or viscous" DNA is as it bends & loops due to all the complex molecular interactions within itself & water
@imperialcollegeldn.bsky.social
@crick.ac.uk
@ucl.ac.uk
⚛️🧪
@imperialcollegeldn.bsky.social
@crick.ac.uk
@ucl.ac.uk
⚛️🧪
Reposted by George Pobegalov
A new material constant of DNA is the major determinant of looping dynamics https://www.biorxiv.org/content/10.1101/2025.07.10.664086v1
July 16, 2025 at 9:48 PM
A new material constant of DNA is the major determinant of looping dynamics https://www.biorxiv.org/content/10.1101/2025.07.10.664086v1
Reposted by George Pobegalov
We found a new asymmetry in the large-scale chromosome structure: sister chromatids are systematically shifted by hundreds of kb in the 5′→3′ direction of their inherited strands! The work was led by Flavia Corsi, in close collaboration with the Daniel Gerlich lab.
www.biorxiv.org/content/10.1...
1/
www.biorxiv.org/content/10.1...
1/
July 15, 2025 at 8:11 AM
We found a new asymmetry in the large-scale chromosome structure: sister chromatids are systematically shifted by hundreds of kb in the 5′→3′ direction of their inherited strands! The work was led by Flavia Corsi, in close collaboration with the Daniel Gerlich lab.
www.biorxiv.org/content/10.1...
1/
www.biorxiv.org/content/10.1...
1/
Reposted by George Pobegalov
I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)
May 23, 2025 at 7:57 PM
I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)
Reposted by George Pobegalov
Earnshaw, Goloborodko, Dekker & Mirny labs are excited to present our latest work, "Rules of engagement for condensins and cohesins guide mitotic chromosome formation" - now accepted!!
www.science.org/doi/10.1126/...
A short clip describing the key results:
www.youtube.com/watch?v=pmvO...
www.science.org/doi/10.1126/...
A short clip describing the key results:
www.youtube.com/watch?v=pmvO...
Rules of Engagement
YouTube video by Johan Gibcus
www.youtube.com
April 11, 2025 at 7:45 AM
Earnshaw, Goloborodko, Dekker & Mirny labs are excited to present our latest work, "Rules of engagement for condensins and cohesins guide mitotic chromosome formation" - now accepted!!
www.science.org/doi/10.1126/...
A short clip describing the key results:
www.youtube.com/watch?v=pmvO...
www.science.org/doi/10.1126/...
A short clip describing the key results:
www.youtube.com/watch?v=pmvO...
Reposted by George Pobegalov
Very pleased to share our new story on SMC complexes, a great team effort from @roisnehamelinf.bsky.social, @taschner.bsky.social in the GruberLab and James of @dnatopologylab.bsky.social We find that SMC hinge domains are ssDNA gates to facilitate obstacle bypass during DNA loop extrusion!
March 18, 2025 at 8:03 AM
Very pleased to share our new story on SMC complexes, a great team effort from @roisnehamelinf.bsky.social, @taschner.bsky.social in the GruberLab and James of @dnatopologylab.bsky.social We find that SMC hinge domains are ssDNA gates to facilitate obstacle bypass during DNA loop extrusion!
Reposted by George Pobegalov
Happy to share the beautiful structure of a Wadjet SMC complex by @roisnehamelinf.bsky.social, with support from the DCI and @drhonsworth.bsky.social ! This is a type II Wadjet with unique characteristics including a tandem KITE subunit, distinct coiled coil architecture and a deviant hinge.
March 11, 2025 at 9:22 AM
Happy to share the beautiful structure of a Wadjet SMC complex by @roisnehamelinf.bsky.social, with support from the DCI and @drhonsworth.bsky.social ! This is a type II Wadjet with unique characteristics including a tandem KITE subunit, distinct coiled coil architecture and a deviant hinge.
Reposted by George Pobegalov
A new PhD position is available in my lab to explore DNA replication mechanisms using single-molecule approaches. Apply by 19th of March:
www.crick.ac.uk/careers-stud...
www.crick.ac.uk/careers-stud...
Yardimci Lab | Single-molecule visualisation of the eukaryotic replication machinery
www.crick.ac.uk
March 4, 2025 at 11:39 AM
A new PhD position is available in my lab to explore DNA replication mechanisms using single-molecule approaches. Apply by 19th of March:
www.crick.ac.uk/careers-stud...
www.crick.ac.uk/careers-stud...
Reposted by George Pobegalov
For anyone that was too busy at the end of last year to think about PhDs or are still looking for the right project/place, I have a project available in the second round of the Yorkshire Biosciences DTP scheme looking at the dynamics of human condensin:
www.findaphd.com/phds/project...
www.findaphd.com/phds/project...
Developing Imaging Solutions to Study the Dynamics of Human Condensin I at University of Sheffield on FindAPhD.com
PhD Project - Developing Imaging Solutions to Study the Dynamics of Human Condensin I at University of Sheffield, listed on FindAPhD.com
www.findaphd.com
February 3, 2025 at 1:52 PM
For anyone that was too busy at the end of last year to think about PhDs or are still looking for the right project/place, I have a project available in the second round of the Yorkshire Biosciences DTP scheme looking at the dynamics of human condensin:
www.findaphd.com/phds/project...
www.findaphd.com/phds/project...
Reposted by George Pobegalov
Congratulations to @mmlab.bsky.social and team, who using the C-Trap, were able to grow microtubules from beads in solution and show that HSET (kinesin-14 family) with microtubule tip-tracking protein can harness forces generated by growing microtubule tips: www.nature.com/articles/s41...
Force-transducing molecular ensembles at growing microtubule tips control mitotic spindle size - Nature Communications
Forces that lead to stable bipolar organization of mitotic spindles are poorly understood. Here the authors show that growing interpolar microtubules make a unique contribution to the overall force ba...
www.nature.com
February 12, 2025 at 3:43 PM
Congratulations to @mmlab.bsky.social and team, who using the C-Trap, were able to grow microtubules from beads in solution and show that HSET (kinesin-14 family) with microtubule tip-tracking protein can harness forces generated by growing microtubule tips: www.nature.com/articles/s41...
Reposted by George Pobegalov
Why do different DNA molecules occupy different places in the nucleus? What drives chromatin compartmentalisation? Read this amazing paper to find out www.science.org/doi/10.1126/...
Many congratulations to all the authors of this brilliant work! @rkoszul.bsky.social
Many congratulations to all the authors of this brilliant work! @rkoszul.bsky.social
February 7, 2025 at 7:18 AM
Why do different DNA molecules occupy different places in the nucleus? What drives chromatin compartmentalisation? Read this amazing paper to find out www.science.org/doi/10.1126/...
Many congratulations to all the authors of this brilliant work! @rkoszul.bsky.social
Many congratulations to all the authors of this brilliant work! @rkoszul.bsky.social
Reposted by George Pobegalov
Exciting news 📃🧬🔬
Today we report on the dynamics of a fascinating class of molecular motors, so-called SMC proteins in @cp-cell.bsky.social
doi.org/10.1016/j.ce...
Today we report on the dynamics of a fascinating class of molecular motors, so-called SMC proteins in @cp-cell.bsky.social
doi.org/10.1016/j.ce...
January 16, 2025 at 4:24 PM
Exciting news 📃🧬🔬
Today we report on the dynamics of a fascinating class of molecular motors, so-called SMC proteins in @cp-cell.bsky.social
doi.org/10.1016/j.ce...
Today we report on the dynamics of a fascinating class of molecular motors, so-called SMC proteins in @cp-cell.bsky.social
doi.org/10.1016/j.ce...
Reposted by George Pobegalov
Come and do your PhD with us @TheCrick and @UCL_IPLS starting October 2025. Apply here: www.ucl.ac.uk/epsrc-doctor...
December 20, 2024 at 2:29 PM
Come and do your PhD with us @TheCrick and @UCL_IPLS starting October 2025. Apply here: www.ucl.ac.uk/epsrc-doctor...
Reposted by George Pobegalov
We measured forces generated in bipolar spindles replacing spindle poles with optical traps!
Read more here: www.nature.com/articles/s41...
Read more here: www.nature.com/articles/s41...
December 17, 2024 at 10:45 AM
We measured forces generated in bipolar spindles replacing spindle poles with optical traps!
Read more here: www.nature.com/articles/s41...
Read more here: www.nature.com/articles/s41...
Reposted by George Pobegalov
Please share: Still plenty of time to apply for PhD projects in my lab using single-molecule methods to look at disordered regions and condensin complexes!
Contact me at [email protected]
For details on how to apply: www.findaphd.com/phds/project...
Contact me at [email protected]
For details on how to apply: www.findaphd.com/phds/project...
Developing Imaging Solutions to Study the Dynamics of Human Condensin I at University of Sheffield on FindAPhD.com
PhD Project - Developing Imaging Solutions to Study the Dynamics of Human Condensin I at University of Sheffield, listed on FindAPhD.com
www.findaphd.com
December 16, 2024 at 3:37 PM
Please share: Still plenty of time to apply for PhD projects in my lab using single-molecule methods to look at disordered regions and condensin complexes!
Contact me at [email protected]
For details on how to apply: www.findaphd.com/phds/project...
Contact me at [email protected]
For details on how to apply: www.findaphd.com/phds/project...
Reposted by George Pobegalov
👀SMC 🛵 on 🧬 induce 🥨 at each➰
www.science.org/doi/10.1126/...
by @ceesdekker.bsky.social @romanbarth.bsky.social et al.
www.science.org/doi/10.1126/...
by @ceesdekker.bsky.social @romanbarth.bsky.social et al.
December 13, 2024 at 8:54 PM
👀SMC 🛵 on 🧬 induce 🥨 at each➰
www.science.org/doi/10.1126/...
by @ceesdekker.bsky.social @romanbarth.bsky.social et al.
www.science.org/doi/10.1126/...
by @ceesdekker.bsky.social @romanbarth.bsky.social et al.
Reposted by George Pobegalov
Happy to see our research on SMC Wadjet published in Molecular Cell. We showed DNA loop extrusion (LE) by a bacterial SMC, confirming LE is conserved across all life forms. Wadjet's LE activity is crucial for recognizing and restricting foreign plasmids, serving as a defense mechanism. 🧬🔬🧪
December 2, 2024 at 4:59 PM
Happy to see our research on SMC Wadjet published in Molecular Cell. We showed DNA loop extrusion (LE) by a bacterial SMC, confirming LE is conserved across all life forms. Wadjet's LE activity is crucial for recognizing and restricting foreign plasmids, serving as a defense mechanism. 🧬🔬🧪
Reposted by George Pobegalov
First attempt in a (loosely defined) Single-Molecule Biophysics starter pack. Please share and (self-) nominate. go.bsky.app/CteFZM5
November 13, 2024 at 8:23 AM
First attempt in a (loosely defined) Single-Molecule Biophysics starter pack. Please share and (self-) nominate. go.bsky.app/CteFZM5