@lucasmkc.bsky.social
5th year graduate student studying genome organization & function | Northwestern University | Backman Lab
Reposted
Our new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1...
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/
March 7, 2025 at 12:37 AM
Our new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1...
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/
Reposted
Among speakers Fri in #seattle,
brain surgeon Jim Olson, MD.
Who helped develop a "tumor paint" from scorpion 🦂
venom with @fredhutch.bsky.social
And @meadekrosby.bsky.social Climate scientist 🌏
Watch thread for more speakers @sufsseattle.bsky.social
🧵
brain surgeon Jim Olson, MD.
Who helped develop a "tumor paint" from scorpion 🦂
venom with @fredhutch.bsky.social
And @meadekrosby.bsky.social Climate scientist 🌏
Watch thread for more speakers @sufsseattle.bsky.social
🧵
March 4, 2025 at 9:33 PM
Among speakers Fri in #seattle,
brain surgeon Jim Olson, MD.
Who helped develop a "tumor paint" from scorpion 🦂
venom with @fredhutch.bsky.social
And @meadekrosby.bsky.social Climate scientist 🌏
Watch thread for more speakers @sufsseattle.bsky.social
🧵
brain surgeon Jim Olson, MD.
Who helped develop a "tumor paint" from scorpion 🦂
venom with @fredhutch.bsky.social
And @meadekrosby.bsky.social Climate scientist 🌏
Watch thread for more speakers @sufsseattle.bsky.social
🧵
Reposted
One of the many, many “pauses” and cancellations devastating our next generation of researchers. “The NIH Intramural Program has paused the recruitment of IRTAs, CRTAs, and Visiting Fellows in all training programs pending guidance from Health and Human Services. Check back daily for updates.”
Postbac Program
www.training.nih.gov
February 18, 2025 at 2:03 AM
One of the many, many “pauses” and cancellations devastating our next generation of researchers. “The NIH Intramural Program has paused the recruitment of IRTAs, CRTAs, and Visiting Fellows in all training programs pending guidance from Health and Human Services. Check back daily for updates.”
Reposted
In effect, our genomes are a very sophisticated computer system. We take measurements of the state (salts, food, stressors), integrate it with our prior measurements (chromatin remodeling from prior exposures, epigenetic memories of our grandparents) and adapt.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Chromatin conformation, gene transcription, and nucleosome remodeling as an emergent system
Gene transcription is an emergent phenomenon of packing domain geometry in situ.
www.science.org
February 6, 2025 at 2:53 PM
In effect, our genomes are a very sophisticated computer system. We take measurements of the state (salts, food, stressors), integrate it with our prior measurements (chromatin remodeling from prior exposures, epigenetic memories of our grandparents) and adapt.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted
Let's start a conversation about this. I keep thinking about how what we do now spans across generations. #Epigenetics and #chromatin is a system for our genomes to tell our cells what to expect in the future and to prepare our children/grandchildren for current-ish conditions.
February 6, 2025 at 2:53 PM
Let's start a conversation about this. I keep thinking about how what we do now spans across generations. #Epigenetics and #chromatin is a system for our genomes to tell our cells what to expect in the future and to prepare our children/grandchildren for current-ish conditions.
Reposted
PREPRINT! Park et al. makes the case that we may be misunderstanding heterochromatin for past 30 years due to ChIP-Seq biases... 1/n
CUT&Tag Identifies Repetitive Genomic Loci that are Excluded from ChIP Assays https://www.biorxiv.org/content/10.1101/2025.02.03.636299v1
February 5, 2025 at 6:34 PM
PREPRINT! Park et al. makes the case that we may be misunderstanding heterochromatin for past 30 years due to ChIP-Seq biases... 1/n
Reposted
Very pleased - on this week of all weeks - to have this paper on the importance of inclusivity in mentorship published. Co-authored with the fantastic Drs. Sadhana Jackson and Jessica Tsai (@jestsai.bsky.social), we do a deep dive on pediatric oncology, but 1/3
www.frontiersin.org/journals/onc...
www.frontiersin.org/journals/onc...
Frontiers | Inclusive mentorship of pediatric trainees: pediatric oncology as a microcosm
www.frontiersin.org
January 31, 2025 at 8:45 PM
Very pleased - on this week of all weeks - to have this paper on the importance of inclusivity in mentorship published. Co-authored with the fantastic Drs. Sadhana Jackson and Jessica Tsai (@jestsai.bsky.social), we do a deep dive on pediatric oncology, but 1/3
www.frontiersin.org/journals/onc...
www.frontiersin.org/journals/onc...
Reposted
Our new manuscript up now! journals.biologists.com/jcs/article/... Nuclear blebs are hallmarks of cancer and aging. The 3-D structure of #chromatin domains inside blebs is completely deformed.
Nuclear blebs are associated with destabilized chromatin packing domains
Disrupted nuclear shape is associated with multiple pathological processes including premature aging disorders, cancer-relevant chromosomal rearrangements, and DNA damage. Nuclear blebs (i.e., herniat...
journals.biologists.com
February 3, 2025 at 3:52 PM
Our new manuscript up now! journals.biologists.com/jcs/article/... Nuclear blebs are hallmarks of cancer and aging. The 3-D structure of #chromatin domains inside blebs is completely deformed.
Reposted
We hypothesized that this unique cohesin-mediated structural change in nascent chromatin domains may be caused by cohesin activity on the surface of mature chromatin domains. Using SMLM to image RAD21 and EDU-labeled chromatin, we found RAD21 primarily localized in DNA-poor areas between domains.
February 3, 2025 at 4:15 PM
We hypothesized that this unique cohesin-mediated structural change in nascent chromatin domains may be caused by cohesin activity on the surface of mature chromatin domains. Using SMLM to image RAD21 and EDU-labeled chromatin, we found RAD21 primarily localized in DNA-poor areas between domains.
Reposted
CPGE's most recent publication on cohesin function and chromatin domain organization is out now in Science Advances. This thread will take a look at some of the key observations from the paper!
#chromatin #genomics #epigenetics #3Dgenome #northwestern #dna #cpge
www.science.org/doi/10.1126/...
#chromatin #genomics #epigenetics #3Dgenome #northwestern #dna #cpge
www.science.org/doi/10.1126/...
Mature chromatin packing domains persist after RAD21 depletion in 3D
RAD21 contributes to nascent packing domain formation, but maturation requires nucleosome posttranslational modifications.
www.science.org
February 3, 2025 at 4:15 PM
CPGE's most recent publication on cohesin function and chromatin domain organization is out now in Science Advances. This thread will take a look at some of the key observations from the paper!
#chromatin #genomics #epigenetics #3Dgenome #northwestern #dna #cpge
www.science.org/doi/10.1126/...
#chromatin #genomics #epigenetics #3Dgenome #northwestern #dna #cpge
www.science.org/doi/10.1126/...
Our paper on cohesin regulation of mesoscale chromatin organization is out
Today in Science Advances: "Mature chromatin packing domains persist after RAD21 depletion in 3D". Researchers at CPGE integrated measurements of genome connectivity and physical structure to deepen our understanding of chromatin organization
#chromatin #genomics
www.science.org/doi/10.1126/...
#chromatin #genomics
www.science.org/doi/10.1126/...
Mature chromatin packing domains persist after RAD21 depletion in 3D
RAD21 contributes to nascent packing domain formation, but maturation requires nucleosome posttranslational modifications.
www.science.org
January 25, 2025 at 12:57 AM
Our paper on cohesin regulation of mesoscale chromatin organization is out
Reposted
There’s a lot to unpack in our recent Science Advances paper - come check it out! Here’s a thread on what we found.
#chromatin #genomics #biophotonics #dna #epigenetics #imaging #northwestern #science #biology #aging #longevity #biomedicine #cpge
www.science.org/doi/10.1126/...
#chromatin #genomics #biophotonics #dna #epigenetics #imaging #northwestern #science #biology #aging #longevity #biomedicine #cpge
www.science.org/doi/10.1126/...
Chromatin conformation, gene transcription, and nucleosome remodeling as an emergent system
Gene transcription is an emergent phenomenon of packing domain geometry in situ.
www.science.org
January 14, 2025 at 5:20 PM
There’s a lot to unpack in our recent Science Advances paper - come check it out! Here’s a thread on what we found.
#chromatin #genomics #biophotonics #dna #epigenetics #imaging #northwestern #science #biology #aging #longevity #biomedicine #cpge
www.science.org/doi/10.1126/...
#chromatin #genomics #biophotonics #dna #epigenetics #imaging #northwestern #science #biology #aging #longevity #biomedicine #cpge
www.science.org/doi/10.1126/...
Reposted
mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription
#RNAbiology #RNAlocalization
www.cell.com/molecular-ce...
#RNAbiology #RNAlocalization
www.cell.com/molecular-ce...
mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription
Transcription inhibitors also disrupt nuclear export. Here, Williams et al. reveal that mRNA export factors sense transcription inhibition and adapt by storing mature export-competent mRNA in nuclear speckles. This enables rapid release when transcription resumes and ensures retention of cellular identity and viability during a transient global transcription insult.
www.cell.com
January 2, 2025 at 11:49 PM
mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription
#RNAbiology #RNAlocalization
www.cell.com/molecular-ce...
#RNAbiology #RNAlocalization
www.cell.com/molecular-ce...
Our latest paper on chromatin domains and genome function is out!
Today in Science Advances: "Chromatin Conformation, Gene Transcription, and Nucleosome Remodeling as an Emergent System" in which CPGE researchers demonstrate that the human genome is an emergent, self-assembling, reinforcement learning system.
#chromatin #genomics
www.science.org/doi/10.1126/...
#chromatin #genomics
www.science.org/doi/10.1126/...
Chromatin conformation, gene transcription, and nucleosome remodeling as an emergent system
Gene transcription is an emergent phenomenon of packing domain geometry in situ.
www.science.org
January 10, 2025 at 8:13 PM
Our latest paper on chromatin domains and genome function is out!
Reposted
Hi Bluesky! I made a 3D chromatin starter pack.
Let me know if you would like to be added.
go.bsky.app/6tTQdqQ
Let me know if you would like to be added.
go.bsky.app/6tTQdqQ
November 13, 2024 at 5:55 PM
Hi Bluesky! I made a 3D chromatin starter pack.
Let me know if you would like to be added.
go.bsky.app/6tTQdqQ
Let me know if you would like to be added.
go.bsky.app/6tTQdqQ
Out here at the #4DNucleome meeting in San Diego with a poster on our recent preprint where we explore the impact of Pol 1 and Pol 2 transcriptional inhibition on mesoscale chromatin organization.
#3DGenome
December 13, 2024 at 2:41 PM
Out here at the #4DNucleome meeting in San Diego with a poster on our recent preprint where we explore the impact of Pol 1 and Pol 2 transcriptional inhibition on mesoscale chromatin organization.
#3DGenome
Reposted
CPGE's archive of recorded talks can be viewed on our YouTube channel at www.youtube.com/@PhysGenCenter.
Visit us at www.physicalgenomics.northwestern.edu for details on our transdisciplinary research platform, training programs, and initiatives such as the Monthly Seminar on Physical Genomics!
Visit us at www.physicalgenomics.northwestern.edu for details on our transdisciplinary research platform, training programs, and initiatives such as the Monthly Seminar on Physical Genomics!
Center for Physical Genomics and Engineering
The mission of the Center for Physical Genomics and Engineering at Northwestern University's McCormick School of Engineering is to create new strategies for the treatment of disease and the reversible...
www.youtube.com
December 12, 2024 at 4:23 AM
CPGE's archive of recorded talks can be viewed on our YouTube channel at www.youtube.com/@PhysGenCenter.
Visit us at www.physicalgenomics.northwestern.edu for details on our transdisciplinary research platform, training programs, and initiatives such as the Monthly Seminar on Physical Genomics!
Visit us at www.physicalgenomics.northwestern.edu for details on our transdisciplinary research platform, training programs, and initiatives such as the Monthly Seminar on Physical Genomics!
Reposted
Single-molecule states link transcription factor binding to gene expression www.nature.com/articles/s41...
Single-molecule states link transcription factor binding to gene expression - Nature
A study uses single-molecule footprinting to measure protein occupancy at regulatory elements on individual molecules in human cells and describes how different properties of transcription factor bind...
www.nature.com
November 20, 2024 at 8:34 PM
Single-molecule states link transcription factor binding to gene expression www.nature.com/articles/s41...