Here is an example of the reversibility. The first 30 min the cell is pulsed with blue light (when blue dot is shown) causing the tagged vimentin to associate with - end oriented kinesins that pull the vimentin to the cell centre. Then blue light pulsing is stopped and vimentin redistributes.

Is this an animated Jackson Pollock painting or live cell tracking movie? 😍

Excited to share our new pre-print showcasing tools (chemical & optogenetic) for repositioning Vimentin in cells! Movie shows ER (Halo-KDEL) sheets being relocalized together with vimentin (Vim) upon local vimentin pulling in U2OS. @gijsjekoenderink.bsky.social
www.biorxiv.org/content/10.1...

This video shows the smallest area I've managed to sever inside a U2OS cell. The goal for this experiment was to sever microtubules around a subset of the Golgi (labelled here in cyan, together with exocytic vesicles) to see what would happen to it's morphology/localization.

Hi! My name is Joyce and I love building and using tools to manipulate the #cytoskeleton of cells to better understand their function. 🔬
Below you can see my movie of #opto-katanin ✂️(SspB-mCh-p60) being used to locally disassemble #microtubules (labelled by SiR-tubulin) inside a neuron.