Here is an example of the reversibility. The first 30 min the cell is pulsed with blue light (when blue dot is shown) causing the tagged vimentin to associate with - end oriented kinesins that pull the vimentin to the cell centre. Then blue light pulsing is stopped and vimentin redistributes.

Excited to share that our fast & reversible chemical and optogenetic vimentin pulling tools are now published in JCB! 🥳
We show a subset of organelles are displaced with vimentin pulling to the cell centre and cells become much softer despite little or no effect on actin/MTs
doi.org/10.1083/jcb....

Is this an animated Jackson Pollock painting or live cell tracking movie? 😍

Come join us in Utrecht for some cutting edge #microscopy and 3D cell biology!! #3Dcellbio

Excited to share our new pre-print showcasing tools (chemical & optogenetic) for repositioning Vimentin in cells! Movie shows ER (Halo-KDEL) sheets being relocalized together with vimentin (Vim) upon local vimentin pulling in U2OS. @gijsjekoenderink.bsky.social
www.biorxiv.org/content/10.1...

This video shows the smallest area I've managed to sever inside a U2OS cell. The goal for this experiment was to sever microtubules around a subset of the Golgi (labelled here in cyan, together with exocytic vesicles) to see what would happen to it's morphology/localization.

Hi! My name is Joyce and I love building and using tools to manipulate the #cytoskeleton of cells to better understand their function. 🔬
Below you can see my movie of #opto-katanin ✂️(SspB-mCh-p60) being used to locally disassemble #microtubules (labelled by SiR-tubulin) inside a neuron.