Curate your database. 🧵4/5
- GTDB, NCBI, ICTV, or custom taxonomy supported.
- Add genomes to a pre-built DB to save time (benchmark in figure)
- Expand taxonomy. e.g., integrate ICTV viruses into a GTDB prokaryote DB.
⏱️Building a DB of 8,520 GTDB species took 106 min on a MacBook M2 Pro (32G)

Explore results with interactive visualization. 🧵3/5
- Generate customized Sankey plots.
- Search for taxa of interest.
- Filter by classified reads or proportion.
- Click taxon nodes for subtree views.
- Extract reads classified to a taxon.
- Access NCBI Taxonomy and genome browser.

The app runs desktop-optimized Metabuli. 🧵2/5
Classifying 2X22M human gut reads vs. 36K genomes over 8,465 species took:
🖥️46 min on a Windows desktop (i9-9900, 32GB RAM)
💻39 min on a MacBook M2 Pro (32GB RAM)

Easy and interactive taxonomic profiling with Metabuli App.
It integrates database curation, read QC, taxonomic profiling, and visualization right on your desktop.
No command line, server, or internet required.
Now published in Bioinformatics! 🧵1/5
doi.org/10.1093/bioi...
github.com/steineggerla...

⚡Metabuli is now even faster on Linux servers and Windows/macOS computers.
Annotating 22M paired-end human gut reads vs. 36K genomes (8,465 GTDB species) took:
🖥️57 min on a Windows desktop (i9-9900, 32GB RAM)
💻39 min on a MacBook M2 Pro (32GB RAM) 🧵4/5

🛠️Curate your database.
- Build DBs with GTDB, NCBI, ICTV, or custom taxonomy.
- Add genomes to pre-built DBs to save time (benchmark in figure)
- Expand taxonomy—e.g., integrate ICTV viruses into a GTDB prokaryote DB.
⏱️Building a DB of 8520 GTDB species took 104 min on a MacBook M2 Pro (32G). 🧵3/5

🔍Explore results with interactive visualization.
- Generate customized Sankey plots.
- Search for taxa of interest.
- Filter by classified reads or proportion.
- Click taxon nodes for subtree views.
- Extract reads classified to a taxon.
- Access NCBI Taxonomy and genome browser. 🧵2/5

Metabuli App preprint is out!
💻Taxonomic classification & interactive visualization—right on your laptop
🛠️Create new databases or update existing ones with new sequences. 🧵1/5
github.com/steineggerla...
www.biorxiv.org/content/10.1101/2025.03.10.642298v1

🛠️Curate your database.
- Build DBs with GTDB, NCBI, ICTV, or custom taxonomy.
- Add genomes to pre-built DBs to save time (benchmark in figure)
- Expand taxonomy—e.g., integrate ICTV viruses into a GTDB prokaryote DB.
⏱️Building a DB of 8,520 GTDB species took 104 min on a MacBook M2 Pro (32G)

🔍Explore results with interactive visualization.
- Generate customized Sankey plots.
- Search for taxa of interest.
- Filter by classified reads or proportion.
- Click taxon nodes for subtree views.
- Extract reads classified to a taxon.
- Access NCBI Taxonomy and genome browser.

Easy taxonomic profiling with the Metabuli App! Explore your samples with publication-ready Sankey and Krona plots. We accelerated Metabuli further, classifying 2x22M reads vs. GTDB in just 43 min on a MacBook Pro M2. 🧵1/4
🚀https://github.com/steineggerlab/Metabuli-App