Carballido-López's lab
@carballido-lab.bsky.social
Reposted by Carballido-López's lab
An SPFH Protein Couples Membrane Stress to Differentiation in Bacillus subtilis https://www.biorxiv.org/content/10.64898/2026.02.06.704340v1
February 8, 2026 at 8:16 AM
An SPFH Protein Couples Membrane Stress to Differentiation in Bacillus subtilis https://www.biorxiv.org/content/10.64898/2026.02.06.704340v1
Reposted by Carballido-López's lab
The characteristics of autolysins associated with cell separation in Bacillus subtilis
-in #JBacteriology
journals.asm.org/doi/10.1128/...
-in #JBacteriology
journals.asm.org/doi/10.1128/...
July 18, 2024 at 7:30 AM
The characteristics of autolysins associated with cell separation in Bacillus subtilis
-in #JBacteriology
journals.asm.org/doi/10.1128/...
-in #JBacteriology
journals.asm.org/doi/10.1128/...
Reposted by Carballido-López's lab
New in JB: Jorgenson reviews recent advances in cell envelope assembly via the lens of the essential lipid carrier undecaprenyl phosphate and with an eye on targets of novel Abx development.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
February 5, 2026 at 2:27 PM
New in JB: Jorgenson reviews recent advances in cell envelope assembly via the lens of the essential lipid carrier undecaprenyl phosphate and with an eye on targets of novel Abx development.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
Reposted by Carballido-López's lab
Please share!!! I am looking for a postdoctoral researcher to join us. Focus will be on exciting collaborative projects on lipids and membrane biology, pushing the boundaries of in silico "reconstitutions". Previous experience with molecular dynamics simulations of biological systems a strong plus!
January 30, 2026 at 9:48 AM
Please share!!! I am looking for a postdoctoral researcher to join us. Focus will be on exciting collaborative projects on lipids and membrane biology, pushing the boundaries of in silico "reconstitutions". Previous experience with molecular dynamics simulations of biological systems a strong plus!
Reposted by Carballido-López's lab
Fer’s tour de force in B. anthracis is out! Fer got Tn-seq running, built an ordered knockout library, defined all essential sporulation genes, and found a peptidoglycan deacetylase inhibitor critical for engulfment. Including our first one-by-all Alphafold screen! journals.plos.org/plosbiology/...
Identification of sporulation genes in Bacillus anthracis highlights similarities and significant differences with Bacillus subtilis
How good is Bacillus subtilis as a model for the spore-forming pathogen Bacillus anthracis? Using high throughput genetics to identify B. anthracis sporulation genes and cytological analysis of the mu...
journals.plos.org
December 13, 2025 at 4:53 PM
Fer’s tour de force in B. anthracis is out! Fer got Tn-seq running, built an ordered knockout library, defined all essential sporulation genes, and found a peptidoglycan deacetylase inhibitor critical for engulfment. Including our first one-by-all Alphafold screen! journals.plos.org/plosbiology/...
Reposted by Carballido-López's lab
The Bacillus subtilis class A penicillin-binding protein 4 (PBP4) requires an accessory protein RpdA www.biorxiv.org/content/10.6...
The Bacillus subtilis class A penicillin-binding protein 4 (PBP4) requires an accessory protein RpdA
Peptidoglycan (PG) synthesis is essential to maintain cell integrity during bacterial growth and division. In Bacillus subtilis, PG synthesis involves class A PBPs that act independently and class B P...
www.biorxiv.org
January 12, 2026 at 2:22 PM
The Bacillus subtilis class A penicillin-binding protein 4 (PBP4) requires an accessory protein RpdA www.biorxiv.org/content/10.6...
Reposted by Carballido-López's lab
A decade later it’s still fun to see this in the wild
not a misconception, but this yt video has stuck with me over the years from a class on evolution taught by kenny smith at Edinburgh. really nice visual of evolution in progress www.youtube.com/watch?v=plVk...
The Evolution of Bacteria on a “Mega-Plate” Petri Dish (Kishony Lab)
YouTube video by Harvard Medical School
www.youtube.com
January 10, 2026 at 2:45 PM
A decade later it’s still fun to see this in the wild
Reposted by Carballido-López's lab
A teichoic acid-like wall modification associated with immune suppression is socially regulated in Streptococcus pyogenes https://www.biorxiv.org/content/10.64898/2026.01.07.698140v1
January 8, 2026 at 8:19 PM
A teichoic acid-like wall modification associated with immune suppression is socially regulated in Streptococcus pyogenes https://www.biorxiv.org/content/10.64898/2026.01.07.698140v1
Reposted by Carballido-López's lab
🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)!
An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools.
🗓️ Apply online by Jan 30, 2026
An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools.
🗓️ Apply online by Jan 30, 2026
Quantitative Imaging: From Acquisition to Analysis
Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.
meetings.cshl.edu
January 6, 2026 at 7:18 PM
🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)!
An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools.
🗓️ Apply online by Jan 30, 2026
An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools.
🗓️ Apply online by Jan 30, 2026
Reposted by Carballido-López's lab
RodZ acts through MreBCD to activate the elongasome in Escherichia coli https://www.biorxiv.org/content/10.64898/2026.01.05.697639v1
January 5, 2026 at 6:17 PM
RodZ acts through MreBCD to activate the elongasome in Escherichia coli https://www.biorxiv.org/content/10.64898/2026.01.05.697639v1
Reposted by Carballido-López's lab
Wetting-Mediated Remodeling of the Cytoskeletal Cortex by Phase-Separated Condensates in Cell-Free Reactions
doi.org/10.1021/acs....
doi.org/10.1021/acs....
Wetting-Mediated Remodeling of the Cytoskeletal Cortex by Phase-Separated Condensates in Cell-Free Reactions
Living cells dynamically coordinate gene expression with the spatial organization of their membranes and cytoskeletons. Unraveling how membrane-less organelles arise and interact with cytoskeletal net...
doi.org
January 2, 2026 at 6:45 PM
Wetting-Mediated Remodeling of the Cytoskeletal Cortex by Phase-Separated Condensates in Cell-Free Reactions
doi.org/10.1021/acs....
doi.org/10.1021/acs....
Reposted by Carballido-López's lab
What is better than one? Two connected papers!
#NewResearch
S protein of Streptococcus pneumoniae activates PBP1a and coordinates with a wider GpsB-associated multi-protein complex to regulate peptidoglycan remodelling and cell division.
#MicroSky 🦠
www.nature.com/articles/s41...
#NewResearch
S protein of Streptococcus pneumoniae activates PBP1a and coordinates with a wider GpsB-associated multi-protein complex to regulate peptidoglycan remodelling and cell division.
#MicroSky 🦠
www.nature.com/articles/s41...
December 19, 2025 at 2:06 PM
What is better than one? Two connected papers!
#NewResearch
S protein of Streptococcus pneumoniae activates PBP1a and coordinates with a wider GpsB-associated multi-protein complex to regulate peptidoglycan remodelling and cell division.
#MicroSky 🦠
www.nature.com/articles/s41...
#NewResearch
S protein of Streptococcus pneumoniae activates PBP1a and coordinates with a wider GpsB-associated multi-protein complex to regulate peptidoglycan remodelling and cell division.
#MicroSky 🦠
www.nature.com/articles/s41...
Reposted by Carballido-López's lab
JB Editor's Choice: Anderson, Khursigara et al. review the role of peptidoglycomics in understanding the structure and function of peptidoglycan.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
December 2, 2025 at 8:54 PM
JB Editor's Choice: Anderson, Khursigara et al. review the role of peptidoglycomics in understanding the structure and function of peptidoglycan.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
Reposted by Carballido-López's lab
wohoo! FtsZ aficionadas/os take note 👇
#MicroSky
#MicroSky
Happy to share a new preprint from the lab: Marko became interested in whether the disordered linker of FtsZ might influence how the protein organizes into the Z-ring. And this led to some surprising findings!
Preprint: www.biorxiv.org/content/10.1...
Preprint: www.biorxiv.org/content/10.1...
Charge segregation in the C-terminal linker of FtsZ enables Z-ring scaling with prokaryotic cell width
Bacterial cytokinesis is orchestrated by the Z-ring, a cytoskeletal structure formed by treadmilling filaments of the tubulin-like GTPase FtsZ. During assembly, filament curvature must match the cell ...
www.biorxiv.org
November 26, 2025 at 10:09 PM
wohoo! FtsZ aficionadas/os take note 👇
#MicroSky
#MicroSky
Reposted by Carballido-López's lab
Really cool paper from @vincentdebakker.bsky.social @jonbakerlab.bsky.social now published @natcomms.nature.com #MicroSky www.nature.com/articles/s41...
Multi-omics profiling reveals atypical sugar utilization and a key membrane composition regulator in Streptococcus pneumoniae - Nature Communications
The pathogen Streptococcus pneumoniae can adapt to diverse microenvironments in the human body. Here, De Bakker et al. study these adaptation responses, showing unusual sugar utilization and identifyi...
www.nature.com
November 25, 2025 at 1:44 PM
Really cool paper from @vincentdebakker.bsky.social @jonbakerlab.bsky.social now published @natcomms.nature.com #MicroSky www.nature.com/articles/s41...
Reposted by Carballido-López's lab
Reposted by Carballido-López's lab
A two-component system signaling hub controls enterococcal membrane remodeling in response to daptomycin https://www.biorxiv.org/content/10.1101/2025.11.16.688641v1
November 17, 2025 at 6:17 PM
A two-component system signaling hub controls enterococcal membrane remodeling in response to daptomycin https://www.biorxiv.org/content/10.1101/2025.11.16.688641v1
Reposted by Carballido-López's lab
Interesting long read.
November 17, 2025 at 7:08 AM
Interesting long read.
Reposted by Carballido-López's lab
Spatial deconstruction of the plasma membrane https://www.biorxiv.org/content/10.1101/2025.11.14.688519v1
November 15, 2025 at 3:48 AM
Spatial deconstruction of the plasma membrane https://www.biorxiv.org/content/10.1101/2025.11.14.688519v1
Reposted by Carballido-López's lab
The cell envelope of diderm bacteria: a unified scaffold, not a stack of layers www.sciencedirect.com/science/arti... #jcampubs
November 10, 2025 at 4:39 PM
The cell envelope of diderm bacteria: a unified scaffold, not a stack of layers www.sciencedirect.com/science/arti... #jcampubs
Reposted by Carballido-López's lab
🔊 New story!
This GlmR (of hope) article has it all. Cell shape, cytokinesis, c-di-AMP, and catalysis - the famous 4 Cs 💎?
There is also antibiotic resistance, phosphorylation, acetylation, and a (cool) model to explain what shapes Bacillus cells.
Excellent group effort. Congrats team!
#Microsky
This GlmR (of hope) article has it all. Cell shape, cytokinesis, c-di-AMP, and catalysis - the famous 4 Cs 💎?
There is also antibiotic resistance, phosphorylation, acetylation, and a (cool) model to explain what shapes Bacillus cells.
Excellent group effort. Congrats team!
#Microsky
Staying in the loop to make ends meet: roles and regulation of GlmR in Bacillus subtilis https://www.biorxiv.org/content/10.1101/2025.11.05.686802v1
November 6, 2025 at 5:06 AM
🔊 New story!
This GlmR (of hope) article has it all. Cell shape, cytokinesis, c-di-AMP, and catalysis - the famous 4 Cs 💎?
There is also antibiotic resistance, phosphorylation, acetylation, and a (cool) model to explain what shapes Bacillus cells.
Excellent group effort. Congrats team!
#Microsky
This GlmR (of hope) article has it all. Cell shape, cytokinesis, c-di-AMP, and catalysis - the famous 4 Cs 💎?
There is also antibiotic resistance, phosphorylation, acetylation, and a (cool) model to explain what shapes Bacillus cells.
Excellent group effort. Congrats team!
#Microsky
Reposted by Carballido-López's lab
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!
www.fpbase.org/spectra/
www.fpbase.org/spectra/
FPbase Fluorescence Spectra Viewer
An interactive fluorescence spectra viewer to evaluate the spectral properties of fluorescent proteins, organic dyes, filters, and detectors.
www.fpbase.org
November 4, 2025 at 3:03 PM
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!
www.fpbase.org/spectra/
www.fpbase.org/spectra/
Reposted by Carballido-López's lab
Genome-wide screen identifies novel factors for surface protein cross-wall trafficking and cell envelope homeostasis in Staphylococcus aureus https://www.biorxiv.org/content/10.1101/2025.11.03.686427v1
November 5, 2025 at 2:16 AM
Genome-wide screen identifies novel factors for surface protein cross-wall trafficking and cell envelope homeostasis in Staphylococcus aureus https://www.biorxiv.org/content/10.1101/2025.11.03.686427v1
Reposted by Carballido-López's lab
Recruitment of the outer-membrane lipoprotein DolP to thedivision site via cardiolipin-mediated diffusion-stateswitching https://www.biorxiv.org/content/10.1101/2025.10.29.685289v1
October 30, 2025 at 2:17 AM
Recruitment of the outer-membrane lipoprotein DolP to thedivision site via cardiolipin-mediated diffusion-stateswitching https://www.biorxiv.org/content/10.1101/2025.10.29.685289v1
Reposted by Carballido-López's lab
Excited to share my latest postdoctoral work in the Shen Lab at Tufts! In this study, we follow up on an exciting finding by former grad student @shailab.bsky.social that C diff uses its Class A PBP (PBP1) to drive cell division (unlike previously studied bacteria)! journals.plos.org/plosgenetics...
Molecular dissection of Class A PBP function uncovers novel features of the non-canonical Clostridioides difficile divisome complex
Author summary Bacterial cell division is an ancient and essential process, but our molecular understanding of this process is primarily based on studies in a select few model systems. Recent work fou...
journals.plos.org
October 28, 2025 at 1:25 AM
Excited to share my latest postdoctoral work in the Shen Lab at Tufts! In this study, we follow up on an exciting finding by former grad student @shailab.bsky.social that C diff uses its Class A PBP (PBP1) to drive cell division (unlike previously studied bacteria)! journals.plos.org/plosgenetics...