Phil Kyriakakis
@breakliquid.bsky.social
Senior Research Scientist at Stanford Bioengineering | Taking apart and building biology.🧪
Update, I grabbed 10 colonies and combined into one @plasmidsaurus.bsky.social reaction. All of them were too small and most were the same, the TE inserted in the same place. So screening colonies won't likely work.
November 22, 2025 at 5:10 AM
Update, I grabbed 10 colonies and combined into one @plasmidsaurus.bsky.social reaction. All of them were too small and most were the same, the TE inserted in the same place. So screening colonies won't likely work.
I tried to post it here
November 21, 2025 at 5:27 PM
I tried to post it here
Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
November 19, 2025 at 6:27 AM
Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
AI can mess things up real fast! 😭
November 15, 2025 at 1:22 AM
AI can mess things up real fast! 😭
I tried to "squeeze gel purify" DNA, sent it for sequencing, and it worked!
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
November 6, 2025 at 6:33 AM
I tried to "squeeze gel purify" DNA, sent it for sequencing, and it worked!
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
iGEM is not your typical scientific conference! @igemcommunity.bsky.social
October 29, 2025 at 7:12 AM
iGEM is not your typical scientific conference! @igemcommunity.bsky.social
I really want to use "behooves me" in this writing (grant), but Grammarly doesn't want me to!
September 26, 2025 at 5:42 PM
I really want to use "behooves me" in this writing (grant), but Grammarly doesn't want me to!
Lalo says happy whatever day it is
September 21, 2025 at 12:05 AM
Lalo says happy whatever day it is
Mr Kitten says happy Saturday
September 20, 2025 at 11:47 PM
Mr Kitten says happy Saturday
If only it fixed my PCR.
September 15, 2025 at 9:46 PM
If only it fixed my PCR.
Your gel running in the IR!
September 15, 2025 at 9:45 PM
Your gel running in the IR!
September 6, 2025 at 9:53 PM
Love seeing this mCherry in sf9 cells!
August 30, 2025 at 9:16 PM
Love seeing this mCherry in sf9 cells!
How cool is it to get messages like that?! Wow! So much of the joy of science is the mentorship I get to give, its the mentees!
August 30, 2025 at 4:24 AM
How cool is it to get messages like that?! Wow! So much of the joy of science is the mentorship I get to give, its the mentees!
I see, the new model had a diffuser sheet and states the diameter in the instruction manual.
August 29, 2025 at 2:53 PM
I see, the new model had a diffuser sheet and states the diameter in the instruction manual.
Finally! Someone recognized my expertise in “topics”
August 18, 2025 at 2:43 PM
Finally! Someone recognized my expertise in “topics”
What does this part of a cytometer do? (The clear cube part)
August 11, 2025 at 11:06 PM
What does this part of a cytometer do? (The clear cube part)
How strange, no “mechanically separated meat”, EDTA or Whale oil?
July 28, 2025 at 1:21 AM
How strange, no “mechanically separated meat”, EDTA or Whale oil?
AI "Final_final_..."
July 24, 2025 at 10:20 PM
AI "Final_final_..."
Regan appointed judge:
June 17, 2025 at 2:53 AM
Regan appointed judge:
They covered these protests?
June 16, 2025 at 2:35 AM
They covered these protests?
