Phil Kyriakakis
@breakliquid.bsky.social
Senior Research Scientist at Stanford Bioengineering | Taking apart and building biology.🧪
Update, I grabbed 10 colonies and combined into one @plasmidsaurus.bsky.social reaction. All of them were too small and most were the same, the TE inserted in the same place. So screening colonies won't likely work.
November 22, 2025 at 5:10 AM
Update, I grabbed 10 colonies and combined into one @plasmidsaurus.bsky.social reaction. All of them were too small and most were the same, the TE inserted in the same place. So screening colonies won't likely work.
Reposted by Phil Kyriakakis
Boolean logic-gated protein cargo release! @coledeforest.bsky.social's group previously used the molecular topology with proteases as inputs to release fluorescent proteins from hydrogels, and they now extended the platform for other protein cargoes! 🧬
onlinelibrary.wiley.com/doi/abs/10.1...
onlinelibrary.wiley.com/doi/abs/10.1...
November 20, 2025 at 12:44 PM
Boolean logic-gated protein cargo release! @coledeforest.bsky.social's group previously used the molecular topology with proteases as inputs to release fluorescent proteins from hydrogels, and they now extended the platform for other protein cargoes! 🧬
onlinelibrary.wiley.com/doi/abs/10.1...
onlinelibrary.wiley.com/doi/abs/10.1...
Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
November 19, 2025 at 6:27 AM
Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
AI can mess things up real fast! 😭
November 15, 2025 at 1:22 AM
AI can mess things up real fast! 😭
I tried to "squeeze gel purify" DNA, sent it for sequencing, and it worked!
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
November 6, 2025 at 6:33 AM
I tried to "squeeze gel purify" DNA, sent it for sequencing, and it worked!
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
iGEM is not your typical scientific conference! @igemcommunity.bsky.social
October 29, 2025 at 7:12 AM
iGEM is not your typical scientific conference! @igemcommunity.bsky.social
“Bang-Bang optimal light control for maximum protein
production in yeast” - love this title
hal.science/hal-05323963...
production in yeast” - love this title
hal.science/hal-05323963...
hal.science
October 26, 2025 at 8:48 PM
“Bang-Bang optimal light control for maximum protein
production in yeast” - love this title
hal.science/hal-05323963...
production in yeast” - love this title
hal.science/hal-05323963...
So many beautiful pictures and I love papers that benchmark a bunch of tools.
How can we see the cells that make up a living organism? Membrane-localising tags can drive fluorescent proteins to the cell's outer membrane, making their outlines visible. But the tags don't work well in all organisms. How do you find one for your species of interest? 🧵
Check our latest preprint
Check our latest preprint
A toolkit for testing membrane-localising tags across species
Transgenic markers and tools have revolutionised how we study cells and developing organisms. Some of the elements needed to construct those tools are universally applicable (e.g. fluorescent proteins...
www.biorxiv.org
October 24, 2025 at 3:17 AM
So many beautiful pictures and I love papers that benchmark a bunch of tools.
If you want your research to sound “basic”, call it “basic research”. - I don’t think the general public will think it is very important.
I like “fundamental science” better. Today I saw “frontier science”, which sounds cooler and more important.
I like “fundamental science” better. Today I saw “frontier science”, which sounds cooler and more important.
better.today
October 20, 2025 at 3:01 PM
If you want your research to sound “basic”, call it “basic research”. - I don’t think the general public will think it is very important.
I like “fundamental science” better. Today I saw “frontier science”, which sounds cooler and more important.
I like “fundamental science” better. Today I saw “frontier science”, which sounds cooler and more important.
Reposted by Phil Kyriakakis
BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)
October 15, 2025 at 12:22 PM
BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)
Reposted by Phil Kyriakakis
A paper in Nature Communication reports on a new method to produce strong, biodegradable plastic from bamboo. The bioplastic resembles oil-based plastics in strength, shapeability, and thermal stability but can biodegrade in soil within 50 days. go.nature.com/4h1xv0X 🧪
October 15, 2025 at 1:50 AM
A paper in Nature Communication reports on a new method to produce strong, biodegradable plastic from bamboo. The bioplastic resembles oil-based plastics in strength, shapeability, and thermal stability but can biodegrade in soil within 50 days. go.nature.com/4h1xv0X 🧪
Reposted by Phil Kyriakakis
🧪 I’d have never guessed it either ⬇️
15 years in the making, we confirmed that mitochondria - the powerhouse of the cell - have an unusual localization in patients who experience psychosis (including schizophrenia and bipolar disorders). You’ll never guess what kind of patient cells we used to make this discovery… 🧵
October 11, 2025 at 9:53 AM
🧪 I’d have never guessed it either ⬇️
@zebrafishrock.bsky.social does anyone here study age-related vision loss in fish? Or vision generally?
October 11, 2025 at 5:49 PM
@zebrafishrock.bsky.social does anyone here study age-related vision loss in fish? Or vision generally?
Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope - Nature Communications
Loretan and colleagues present a low-cost smartphone-based microscope capable of detecting single-molecule fluorescence. This approach opens doors to personalised and widely distributed applications in diagnostics, biosensing, and science education.
www.nature.com
October 10, 2025 at 3:08 AM
Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Smile 😃
#APOD Astronomy Picture of the Day
SN Encore: A Second Supernova Seen Several Times
Credits: NASA, ESA, CSA, STScI, J. Pierel (STScI) & A. Newman (Carnegie Inst. for Science) 🧪🔭
www.star.ucl.ac.uk/~apod/apod/a...
SN Encore: A Second Supernova Seen Several Times
Credits: NASA, ESA, CSA, STScI, J. Pierel (STScI) & A. Newman (Carnegie Inst. for Science) 🧪🔭
www.star.ucl.ac.uk/~apod/apod/a...
October 7, 2025 at 3:02 PM
Smile 😃
Reposted by Phil Kyriakakis
🧪"In doing so, Arnold has mastered the art of a good apology: one that doesn’t veer into excusing oneself; that gives context where relevant; but accepts full responsibility and moves on".
www.theguardian.com/science/2020...
www.theguardian.com/science/2020...
Nobel prize winner demonstrates the best way to apologize
Unfortunately there aren’t Nobel prizes for good apologies – but Dr Frances Arnold’s words should be an example to all of us
www.theguardian.com
October 7, 2025 at 2:07 AM
🧪"In doing so, Arnold has mastered the art of a good apology: one that doesn’t veer into excusing oneself; that gives context where relevant; but accepts full responsibility and moves on".
www.theguardian.com/science/2020...
www.theguardian.com/science/2020...
Reposted by Phil Kyriakakis
Their efficacy was confirmed against LIVE viruses including Ebola, Marburg, Nipah, Hendra, SARS-CoV, SARS-CoV-2 (COVID-19), AND Middle East Respiratory Syndrome (MERS).
The study has been published in Science Advances. YES, it is PEER-REVIEWED.
• www.science.org/doi/10.1126/...
🧪🧵⬇️
The study has been published in Science Advances. YES, it is PEER-REVIEWED.
• www.science.org/doi/10.1126/...
🧪🧵⬇️
October 4, 2025 at 4:02 PM
Their efficacy was confirmed against LIVE viruses including Ebola, Marburg, Nipah, Hendra, SARS-CoV, SARS-CoV-2 (COVID-19), AND Middle East Respiratory Syndrome (MERS).
The study has been published in Science Advances. YES, it is PEER-REVIEWED.
• www.science.org/doi/10.1126/...
🧪🧵⬇️
The study has been published in Science Advances. YES, it is PEER-REVIEWED.
• www.science.org/doi/10.1126/...
🧪🧵⬇️
Reposted by Phil Kyriakakis
Got to love studies that were clearly conducted "for the love of the game" (“self-decapitating sea slugs”, “plants that see and use that for mimicry”, “worms that jump in the air”, aka papers you sometimes find in @currentbiology.bsky.social
) References to all of this bellow👇
) References to all of this bellow👇
September 29, 2025 at 6:15 AM
Got to love studies that were clearly conducted "for the love of the game" (“self-decapitating sea slugs”, “plants that see and use that for mimicry”, “worms that jump in the air”, aka papers you sometimes find in @currentbiology.bsky.social
) References to all of this bellow👇
) References to all of this bellow👇
I hope my algorithm can regenerate too
It's probably the deterioration of social media (I hope) but i'm exposed to a lot less cool science. It used to feel like a new cool study is being preprinted/published every week, and lately it's rare (at least so it seems). Share a recent study that's worth knowing!
September 27, 2025 at 6:15 PM
I hope my algorithm can regenerate too
I really want to use "behooves me" in this writing (grant), but Grammarly doesn't want me to!
September 26, 2025 at 5:42 PM
I really want to use "behooves me" in this writing (grant), but Grammarly doesn't want me to!
Lalo says happy whatever day it is
September 21, 2025 at 12:05 AM
Lalo says happy whatever day it is
Mr Kitten says happy Saturday
September 20, 2025 at 11:47 PM
Mr Kitten says happy Saturday
What else should I record with IR?
September 16, 2025 at 2:25 AM
What else should I record with IR?