Sebastian S. Cocioba
@atinygreencell.bsky.social
Flower Designer at Neoplants 🌺
Researcher at Binomica Labs
Degreeless Heathen
Petunia and Snapdragon Breeder
Open Lab Notebook: http://tinyurl.com/ATGCFFE
Researcher at Binomica Labs
Degreeless Heathen
Petunia and Snapdragon Breeder
Open Lab Notebook: http://tinyurl.com/ATGCFFE
Mom loved watching the pipetting robot run. Also peep the sport fishing shirt; my late dad's she uses as a gardening shirt.
November 20, 2025 at 5:32 PM
Mom loved watching the pipetting robot run. Also peep the sport fishing shirt; my late dad's she uses as a gardening shirt.
Waking up the mixologist...
November 20, 2025 at 3:34 PM
Waking up the mixologist...
I *love* flowers so much. Look at my lil Alice goooooo. Proud dad. 🥰💚
November 19, 2025 at 5:44 PM
I *love* flowers so much. Look at my lil Alice goooooo. Proud dad. 🥰💚
I feel like this could be used for science somehow, but also baking is science and I want an excuse to make statistically significant inroads into the ideal pancake recipe lol
November 19, 2025 at 2:39 PM
I feel like this could be used for science somehow, but also baking is science and I want an excuse to make statistically significant inroads into the ideal pancake recipe lol
Truly the most important thing of this entire gig, by far. I haven't had real health insurance in YEARS. More than anything I'm so thankful for the chance to take care of myself. Worth every penny.
November 19, 2025 at 5:26 AM
Truly the most important thing of this entire gig, by far. I haven't had real health insurance in YEARS. More than anything I'm so thankful for the chance to take care of myself. Worth every penny.
"We have rupture disks at home..."
Found a nice bladed gizmo that cuts perfect circles of anything up to 0.4mm thick with a tungsten blade. Very clean once you get the blade depth dialed in. Identical disks. Time to test all the sheet plastics, lol.
a.co/d/2daeOJK
Found a nice bladed gizmo that cuts perfect circles of anything up to 0.4mm thick with a tungsten blade. Very clean once you get the blade depth dialed in. Identical disks. Time to test all the sheet plastics, lol.
a.co/d/2daeOJK
November 18, 2025 at 9:42 PM
"We have rupture disks at home..."
Found a nice bladed gizmo that cuts perfect circles of anything up to 0.4mm thick with a tungsten blade. Very clean once you get the blade depth dialed in. Identical disks. Time to test all the sheet plastics, lol.
a.co/d/2daeOJK
Found a nice bladed gizmo that cuts perfect circles of anything up to 0.4mm thick with a tungsten blade. Very clean once you get the blade depth dialed in. Identical disks. Time to test all the sheet plastics, lol.
a.co/d/2daeOJK
My battle flag
November 18, 2025 at 1:17 AM
My battle flag
White snapdragon mutant seed pod lookin HEALTHY. Definitely selfable. Fingers crossed a few of the others also take. Gonna self all the other flowers later this evening after my first shift at Neoplants is done. So excited to make some very cool plants!!!
November 17, 2025 at 4:20 PM
White snapdragon mutant seed pod lookin HEALTHY. Definitely selfable. Fingers crossed a few of the others also take. Gonna self all the other flowers later this evening after my first shift at Neoplants is done. So excited to make some very cool plants!!!
Yup, right is RUBY driven by 35s and left petal is RUBY driven by my broken lotus ubiquitin promoter. Gonna fix the 3' intron and try again. Should be very strong once its fixed. Dang introns, lol.
November 16, 2025 at 5:52 PM
Yup, right is RUBY driven by 35s and left petal is RUBY driven by my broken lotus ubiquitin promoter. Gonna fix the 3' intron and try again. Should be very strong once its fixed. Dang introns, lol.
Arrg, 3bp messed up my experiments lol. I was having problems getting any expression from this UBQ1 promoter from lotus, and it turns out the promoter is actually promoter+exon1+INTRON where the YAG acceptor is CRITICAL for proper splicing. Wasted 1 month on 3bp; fixed it now.
November 15, 2025 at 11:04 PM
Arrg, 3bp messed up my experiments lol. I was having problems getting any expression from this UBQ1 promoter from lotus, and it turns out the promoter is actually promoter+exon1+INTRON where the YAG acceptor is CRITICAL for proper splicing. Wasted 1 month on 3bp; fixed it now.
I heard y'all like cells...
Petunia leaf disk cells undergoing swelling due to exogenous phyohormones. Quite the departure from the jigsaw puzzle piece shape of normal plant leaf cells.
Petunia leaf disk cells undergoing swelling due to exogenous phyohormones. Quite the departure from the jigsaw puzzle piece shape of normal plant leaf cells.
November 15, 2025 at 8:10 PM
I heard y'all like cells...
Petunia leaf disk cells undergoing swelling due to exogenous phyohormones. Quite the departure from the jigsaw puzzle piece shape of normal plant leaf cells.
Petunia leaf disk cells undergoing swelling due to exogenous phyohormones. Quite the departure from the jigsaw puzzle piece shape of normal plant leaf cells.
I know we are shutdown but wow are US court document databases slow to register new users. Waiting for my PACER account to be updated so I can read the actual Center For Food Safety vs USDA APHIS case that changed everything. Meanwhile my account authorized for days now...
November 15, 2025 at 1:45 AM
I know we are shutdown but wow are US court document databases slow to register new users. Waiting for my PACER account to be updated so I can read the actual Center For Food Safety vs USDA APHIS case that changed everything. Meanwhile my account authorized for days now...
Like this is exactly how the previous whisker run began to form at around the same 48hr mark. Seen here is the most notable leaf disk from the PLAS POS cohort, which was incubated for 30mins in plasmolysis buffer followed by 60sec vortex at max speed. Vortexed all the treatments so....interestinggg
November 14, 2025 at 4:00 PM
Like this is exactly how the previous whisker run began to form at around the same 48hr mark. Seen here is the most notable leaf disk from the PLAS POS cohort, which was incubated for 30mins in plasmolysis buffer followed by 60sec vortex at max speed. Vortexed all the treatments so....interestinggg
I love the industrial atmo and string synergy in Nigel's work. One of my favorite albums for deep work.
November 14, 2025 at 3:55 PM
I love the industrial atmo and string synergy in Nigel's work. One of my favorite albums for deep work.
Oooooh, so it seems the plasmolysis buffer + vortexing, even without any whiskers or binding buffer, seems to be causing some necrosis.
MS NEG = MS media, no vortex
MS POS = MS media, yes vortex
PLAS NEG = plasmolysis, no vortex
PLAS POS = plasmolysis, yes vortex
Plot thickens!
MS NEG = MS media, no vortex
MS POS = MS media, yes vortex
PLAS NEG = plasmolysis, no vortex
PLAS POS = plasmolysis, yes vortex
Plot thickens!
November 14, 2025 at 3:12 PM
Oooooh, so it seems the plasmolysis buffer + vortexing, even without any whiskers or binding buffer, seems to be causing some necrosis.
MS NEG = MS media, no vortex
MS POS = MS media, yes vortex
PLAS NEG = plasmolysis, no vortex
PLAS POS = plasmolysis, yes vortex
Plot thickens!
MS NEG = MS media, no vortex
MS POS = MS media, yes vortex
PLAS NEG = plasmolysis, no vortex
PLAS POS = plasmolysis, yes vortex
Plot thickens!
Precious lil petunia paws
November 14, 2025 at 4:45 AM
Precious lil petunia paws
OMG MY ACYANIC SNAPDRAGON MUTANT IS SELF COMPATIBLE!!!
All my other ones need to be crossed since they have an s-locus self incompatibility.
THIS ONE DOESN'T????
Reference:
academic.oup.com/mbe/article/...
All my other ones need to be crossed since they have an s-locus self incompatibility.
THIS ONE DOESN'T????
Reference:
academic.oup.com/mbe/article/...
November 12, 2025 at 8:09 PM
OMG MY ACYANIC SNAPDRAGON MUTANT IS SELF COMPATIBLE!!!
All my other ones need to be crossed since they have an s-locus self incompatibility.
THIS ONE DOESN'T????
Reference:
academic.oup.com/mbe/article/...
All my other ones need to be crossed since they have an s-locus self incompatibility.
THIS ONE DOESN'T????
Reference:
academic.oup.com/mbe/article/...
I then plated the disks onto MS media with hormones (BAP 1mg, NAA 0.1mg), one set of 6 disks per plate with corresponding labeling to track treatments. This is obviously a low powered experiment intended as a feeler for a larger run later. Let's see what happens!
November 12, 2025 at 5:01 PM
I then plated the disks onto MS media with hormones (BAP 1mg, NAA 0.1mg), one set of 6 disks per plate with corresponding labeling to track treatments. This is obviously a low powered experiment intended as a feeler for a larger run later. Let's see what happens!
To the tubes labeled M+ and P+ I added an extra step of vortexing for 60 seconds immediately following the 30min room temp incubation.
November 12, 2025 at 5:01 PM
To the tubes labeled M+ and P+ I added an extra step of vortexing for 60 seconds immediately following the 30min room temp incubation.
In this experiment I tested if the plasmolysis buffer incu for 30 mins with or without vortexing for 60sec caused any similar wounding. I sterilized some leaves with NaDCC, made four sets of 6 disks, placed each set in a tube of either MS liquid media or plasmolysis buffer.
November 12, 2025 at 5:01 PM
In this experiment I tested if the plasmolysis buffer incu for 30 mins with or without vortexing for 60sec caused any similar wounding. I sterilized some leaves with NaDCC, made four sets of 6 disks, placed each set in a tube of either MS liquid media or plasmolysis buffer.
Carbide Whisker Experiment #2 underway!
Exploring the effects of plasmolysis and/or vortexing on necrosis in petunia leaf disks. I want to systematically go through the variables and attempt to link cause to the necrosis I saw in the first experiment. 24hrs in and disks healthy!
Exploring the effects of plasmolysis and/or vortexing on necrosis in petunia leaf disks. I want to systematically go through the variables and attempt to link cause to the necrosis I saw in the first experiment. 24hrs in and disks healthy!
November 12, 2025 at 4:55 PM
Carbide Whisker Experiment #2 underway!
Exploring the effects of plasmolysis and/or vortexing on necrosis in petunia leaf disks. I want to systematically go through the variables and attempt to link cause to the necrosis I saw in the first experiment. 24hrs in and disks healthy!
Exploring the effects of plasmolysis and/or vortexing on necrosis in petunia leaf disks. I want to systematically go through the variables and attempt to link cause to the necrosis I saw in the first experiment. 24hrs in and disks healthy!
Blarg, I needed a positive control for my whacky tungsten gene gun experiments so I caved and bought some premade gold. Enough for about 100 shots. Expensive but adds a critical control to my experiments so I'm not literally shooting in the dark. My wallet tho 😭
November 12, 2025 at 12:39 AM
Blarg, I needed a positive control for my whacky tungsten gene gun experiments so I caved and bought some premade gold. Enough for about 100 shots. Expensive but adds a critical control to my experiments so I'm not literally shooting in the dark. My wallet tho 😭
Big scam's afoot. Block and report for clear ToS violation. Fly safe, friends!
November 11, 2025 at 11:51 PM
Big scam's afoot. Block and report for clear ToS violation. Fly safe, friends!
Decent minimalist vortexer with touch activation. $25 on Amazon. Fixed speed but it can sling a 50mL to a full tornado and thats enough for me. Force needed low enough to touch vortex an open eppi tube with one hand (my very specific genegun prep needs). 12v generic barrel jack. Nice
a.co/d/bkrYeEz
a.co/d/bkrYeEz
November 11, 2025 at 11:16 PM
Decent minimalist vortexer with touch activation. $25 on Amazon. Fixed speed but it can sling a 50mL to a full tornado and thats enough for me. Force needed low enough to touch vortex an open eppi tube with one hand (my very specific genegun prep needs). 12v generic barrel jack. Nice
a.co/d/bkrYeEz
a.co/d/bkrYeEz
Crossing blooms is my happy place 💚🌺
November 11, 2025 at 11:04 PM
Crossing blooms is my happy place 💚🌺