I like this movie, but a friend of mine likes to complain about the obvious stitching artifacts. I'll try harder next time, Michael. Vimentin (orange) and ER (blue) in an overnight acquisition.

Still posting cytoskeleton videos, it seems. Actin this time.

Sample: Lifeact-eGFP in HeLa cells.
Modality: Airyscan confocal

Timestamp is mm:ss and the scale bar is 5 µm.

I mean…does this work? I’m at my wits end.

Attempt number seven at uploading this video of intermediate filaments in an enormous COS7 cell. I have a feeling the BlueSky compression will not do it any favors.

This is an airyscan confocal movie of mitochondria (white) moving around in a mouse astrocyte. Actin filaments are in orange and microtubules are in blue.

This is a cumulative maximum intensity projection movie of the endoplasmic reticulum labeled with the membrane marker mEmerald-Sec61B.

Actin sun.

FilaBuster - Vimentin IF fragmentation

Happy to officially introduce FilaBuster - a strategy for rapid, light-mediated intermediate filament disassembly. Compatible with multiple IF types, modular in design, and precise enough to induce localized filament disassembly in live cells.

www.biorxiv.org/content/10.1...

In the paper, we introduce and characterize a new tool for tracking single vimentin IFs in live cells. After noticing single filaments moving independently within ostensibly tight bundles, we used FIB-SEM to take a closer look at vimentin bundle organization and vimentin-microtubule interactions.

Poor little vimentin stick man 😱

A couple of months later than expected, but I'm finally gearing up to share *FilaBuster* - our approach for light-mediated IF disassembly. Stay tuned!

Really bummed to have missed ASCB this year due to some last-minute circumstances, but excited that our manuscript describing an IF disassembly tool will (hopefully) be posted soon. Here's a movie of vimentin color-coded by orientation.

Vimentin-mSG FRAP

This is a movie of actin filaments in a cultured cell. I like watching it and I hope you do to.

It's not a repost if it's a new platform, right? right??
Lattice light sheet movie of actin in mitosis. Blebby.

FFmpeg spent substantially more time on this one. Let's see if the upload looks any better.

MP4 test. Please ignore.

These are intermediate filaments reconstructed from FIB-SEM but it doesn't matter. Let's say it's pasta. Just trying to work out which videos upload best.

OK swing and a miss on the GIF. Trying Mp4 again but smaller.