it’s a little transparent, but that’s the scale bar in the bottom left corner. 5 micrometers.

Yup - imaged it yesterday on a laser scanning confocal.

Yup!

Fun fact - this protein belongs to the same family as the hard keratins found in all hair (including the hair you might find in a shower drain).

We should promote IFs and septins to position 1 and 2. Actin filaments and microtubules have had their day in the sun. We can just start calling them the third and fourth components 😂

Sure - these are vimentin intermediate filaments which are part of the cell’s internal scaffolding. They confer mechanical resilience to cells and , among other things, help to anchor and position organelles like mitochondria. These filaments are part of the same family that includes keratins.

Not rude at all - appreciate the suggestion. I’ll give it a shot.

Ah sorry for the lack of context. Cos7 cells are a cultured cell line originally derived from African green monkey kidney cells. They are exceptionally flat at the cell periphery so imagers like to use them. This one is a bit of a monster - it’s about 5x larger than normal.

Love it. I acquired the movie at about 10 seconds per frame and the whole thing is just over 15 minutes.

I mean…does this work? I’m at my wits end.

Thanks! This was actually all dyes. Mito tracker green, SPY555-Tubulin, and SPY650-FastAct-X.

Whoops forgot to add that - 5 microns

It’s sped up - the timer is minutes:seconds