Polyamines prevent the action of kinases on acidic phosphorylatable motifs in spliceosomal proteins, thus providing a mechanism for metabolite-mediated regulation of alternative splicing in cells.

Understanding how cells make decisions over time requires the ability to link past molecular states to future phenotypic outcomes. We present TimeVault, a genetically encoded system that records and s...

Several luciferases have been developed for imaging and biosensing, and the collection continues to grow as new applications are pursued. The current workflow for luciferase optimization, while successful, remains laborious and inefficient. Mutant libraries are generated in vitro and screened, “winning” mutants are picked by hand, and the isolated sequences are subjected to additional rounds of mutagenesis and screening. Here, we present a streamlined platform for luciferase engineering that removes the need for manual library generation during each cycle. We purposed an orthogonal DNA replication (OrthoRep) system for continuous hypermutation of a well-known luciferase (GeNL). Short cycles of culturing and screening were sufficient to evolve the enzyme, with no repetitive manual library generation necessary. New GeNL variants were identified that exhibit improved light outputs with a noncognate and inexpensive luciferin. We further characterized the novel luciferases in cell models. Collectively this work establishes OrthoRep and continuous hypermutation as a viable method to engineer luciferases, and sets the stage for more rapid development of bioluminescent reporters.

Daily minimum temperature (Tmin) is increasing faster than maximum temperature. However its impact on the physiology of crop yield and adaptation is barely studied. Using 42 years of annual yield trials at 255 sites representing most spring wheat production regions, the effects of environmental covariates were examined. During grainfilling, average Tmin explained 40% of yield variation, and 52% considering radiation; generally an increase of 1 degree C reduces yield ~0.5 t/ha. The relation between yield was linear over a 14 degree C range. An average increase of 1.2 degree C at the test sites reduced yield by more than 10%. A shortened grainfilling period is part of the explanation; nocturnal rates of dark respiration are also likely factors. Improvement of adaptation to warmer nights could generate a step-change in wheat yield, the most widely-grown crop globally, covering more than 220 million ha, and providing 20% of human calories and protein. ### Competing Interest Statement The authors have declared no competing interest. Digital Transformation and the Climate Action Science Programs of CGIAR International Wheat Yield Partnership (IWYP) funded by the Biotechnology and Biological Research Council of the UK

Background Open chromatin regions host DNA regulatory motifs that are accessible to transcription factors and the transcriptional machinery. In Arabidopsis, responses to light are heavily regulated at...

PhD Project - AI RNA Design for Developing Antiviral Solutions in Sugar Beet (Plant BioDesign JIC project) at University of York, listed on FindAPhD.com

Pattern-triggered immunity (PTI) is initiated when plants detect pathogen-associated molecular patterns (PAMPs) through pattern-recognition receptors (PRRs). How moderate increases in temperature affe...