Peter Cullen
@peterjcullen1.bsky.social
University Researcher, All things: Endosomes, Lysosomes, Retromer, Retriever, Sorting nexins, Commander, RABs, et al.
Reposted by Peter Cullen
Add our first pre-print to your reading list?
Using AF2 and MD, we've characterised structures and conformational ensembles of human rhomboid proteases - and revealed some surprises along the way. Feel v.lucky to work alongside Bryony & @robincorey.bsky.social!
Please share: tinyurl.com/3h8r7f5y
Using AF2 and MD, we've characterised structures and conformational ensembles of human rhomboid proteases - and revealed some surprises along the way. Feel v.lucky to work alongside Bryony & @robincorey.bsky.social!
Please share: tinyurl.com/3h8r7f5y
November 25, 2025 at 9:57 AM
Add our first pre-print to your reading list?
Using AF2 and MD, we've characterised structures and conformational ensembles of human rhomboid proteases - and revealed some surprises along the way. Feel v.lucky to work alongside Bryony & @robincorey.bsky.social!
Please share: tinyurl.com/3h8r7f5y
Using AF2 and MD, we've characterised structures and conformational ensembles of human rhomboid proteases - and revealed some surprises along the way. Feel v.lucky to work alongside Bryony & @robincorey.bsky.social!
Please share: tinyurl.com/3h8r7f5y
Excited to announce, the 93rd Biochemical Society Harden Conference will be on "Endosomal-lysosomal network dysfunction in neurodegeneration". Come and join us in early September on the beautiful south coast of England. Lots of speaker slots available from submitted abstracts!
November 20, 2025 at 4:37 PM
Excited to announce, the 93rd Biochemical Society Harden Conference will be on "Endosomal-lysosomal network dysfunction in neurodegeneration". Come and join us in early September on the beautiful south coast of England. Lots of speaker slots available from submitted abstracts!
Latest endosomal publication with @brettcollins.bsky.social: Rebeka Butkovic's beautiful work identifying a new Commander assembly, the RAB32-LRMDA-Commander, and its role in melanosome biogenesis. Reveals molecular mechanism of human oculocutaneous albinism type 7. www.nature.com/articles/s41...
Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7
Nature Communications - Membrane trafficking is essential for organelle biogenesis. Here, the authors discover an alternative assembly of the Commander trafficking complex and show its role in...
www.nature.com
October 2, 2025 at 10:30 AM
Latest endosomal publication with @brettcollins.bsky.social: Rebeka Butkovic's beautiful work identifying a new Commander assembly, the RAB32-LRMDA-Commander, and its role in melanosome biogenesis. Reveals molecular mechanism of human oculocutaneous albinism type 7. www.nature.com/articles/s41...
Reposted by Peter Cullen
Structures of EHD2 filaments on curved membranes provide a model for caveolar neck stabilization https://www.biorxiv.org/content/10.1101/2025.06.05.658037v1
June 7, 2025 at 1:17 PM
Structures of EHD2 filaments on curved membranes provide a model for caveolar neck stabilization https://www.biorxiv.org/content/10.1101/2025.06.05.658037v1
Reposted by Peter Cullen
Pleased to see Kevin's paper on the Retromer complex interaction with yeast Vps5 and Vps17 proteins now online!
Molecular basis for the assembly of the Vps5-Vps17 SNX-BAR proteins with Retromer
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Molecular basis for the assembly of the Vps5-Vps17 SNX-BAR proteins with Retromer - Nature Communications
Retromer complex cooperates with Vps5 and Vps17 in yeast to traffic transmembrane proteins. Here, the authors present structural and functional studies that reveal the mechanism of Retromer and Vps5 i...
www.nature.com
April 16, 2025 at 1:59 AM
Pleased to see Kevin's paper on the Retromer complex interaction with yeast Vps5 and Vps17 proteins now online!
Reposted by Peter Cullen
Moving obituary of David Stephens, "in many ways, the perfect academic" journals.biologists.com/jcs/article/...
April 15, 2025 at 3:33 PM
Moving obituary of David Stephens, "in many ways, the perfect academic" journals.biologists.com/jcs/article/...
Reposted by Peter Cullen
New preprint on kinesin inhibition and activation - collaborative effort across Bristol Biochem and Chemistry with Woolfson and Schaffizel labs and JJ Phillips in Exeter 1/2 www.biorxiv.org/content/10.1...
Molecular mechanism for cargo-mediated initiation of kinesin-1 activation
Kinesin-1 is a dynamic heterotetrameric assembly of two heavy and two light chains (KHC and KLC) that mediates microtubule-based intracellular transport of many different cargoes. The complex adopts a...
www.biorxiv.org
April 10, 2025 at 1:02 PM
New preprint on kinesin inhibition and activation - collaborative effort across Bristol Biochem and Chemistry with Woolfson and Schaffizel labs and JJ Phillips in Exeter 1/2 www.biorxiv.org/content/10.1...
Reposted by Peter Cullen
Pls re-skeet! There's a postdoc job available to join us in Bristol! We're looking for a molecular cell biologist to help us investigate plasma membrane proteostasis in neurons. Apply here by 23 April: www.jobs.ac.uk/job/DMO097/r.... If interested, do contact me and check out grieve-lab.com! Thanks!
April 9, 2025 at 3:42 PM
Pls re-skeet! There's a postdoc job available to join us in Bristol! We're looking for a molecular cell biologist to help us investigate plasma membrane proteostasis in neurons. Apply here by 23 April: www.jobs.ac.uk/job/DMO097/r.... If interested, do contact me and check out grieve-lab.com! Thanks!
Reposted by Peter Cullen
SNX-BAR proteins 5 and 6 are required for NCOA7-AS antiviral activity against influenza A virus https://www.biorxiv.org/content/10.1101/2025.04.01.646557v1
April 2, 2025 at 4:17 AM
SNX-BAR proteins 5 and 6 are required for NCOA7-AS antiviral activity against influenza A virus https://www.biorxiv.org/content/10.1101/2025.04.01.646557v1
Reposted by Peter Cullen
Lysosomal acidification is key for cellular health, with impairment linked to aging & neurodegenerative diseases. Lin et al. identify USP45 as a negative regulator of autophagy, inhibiting V-ATPase localization to autolysosomes via Coronin 1B-actin modulation. rupress.org/jcb/article/...
March 11, 2025 at 5:03 PM
Lysosomal acidification is key for cellular health, with impairment linked to aging & neurodegenerative diseases. Lin et al. identify USP45 as a negative regulator of autophagy, inhibiting V-ATPase localization to autolysosomes via Coronin 1B-actin modulation. rupress.org/jcb/article/...
Reposted by Peter Cullen
Interested how events are coordinated during #endosome maturation? Check out our latest paper! Congrats Daniel, Samit and Jachen!
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
Coordination between ESCRT function and Rab conversion during endosome maturation | The EMBO Journal
imageimageEarly endosome maturation requires Rab conversion and ESCRT function; however, how
these processes are coordinated remains largely elusive. This study proposes that
Rab conversion in both C....
www.embopress.org
February 5, 2025 at 3:53 PM
Interested how events are coordinated during #endosome maturation? Check out our latest paper! Congrats Daniel, Samit and Jachen!
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
Two new lab manuscripts describing new insight into VPS29’s role in Retromer vs Retriever, and the identification of a non-classical Commander pathway in melanosome biogenesis. Both with @BrettCollins.bsky.social lab. www.biorxiv.org/content/10.1... and www.biorxiv.org/content/10.1...
Identification of a VPS29 isoform with restricted association to Retriever and Retromer accessory proteins through auto-inhibition.
The endosomal-lysosomal network is a hub of organelles that orchestrate the dynamic sorting of hundreds of integral membrane proteins to maintain cellular homeostasis. VPS29 is a central conductor of ...
www.biorxiv.org
February 4, 2025 at 9:08 PM
Two new lab manuscripts describing new insight into VPS29’s role in Retromer vs Retriever, and the identification of a non-classical Commander pathway in melanosome biogenesis. Both with @BrettCollins.bsky.social lab. www.biorxiv.org/content/10.1... and www.biorxiv.org/content/10.1...
Reposted by Peter Cullen
Identification of a VPS29 isoform with restricted association to Retriever and Retromer accessory proteins through auto-inhibition. https://www.biorxiv.org/content/10.1101/2025.02.04.636264v1
February 4, 2025 at 4:31 PM
Identification of a VPS29 isoform with restricted association to Retriever and Retromer accessory proteins through auto-inhibition. https://www.biorxiv.org/content/10.1101/2025.02.04.636264v1
Reposted by Peter Cullen
Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7 https://www.biorxiv.org/content/10.1101/2025.02.04.636395v1
February 4, 2025 at 4:30 PM
Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7 https://www.biorxiv.org/content/10.1101/2025.02.04.636395v1
This is a fantastic route for international post docs to perform research in a UK lab. Bristol Biochemistry would be a great home, including our own endosomal-lysosomal lab. royalsociety.org/grants/newto...
Newton International Fellowships | Royal Society
This fellowship is for non-UK scientists who are at an early stage of their research career and wish to conduct research in the UK.
royalsociety.org
January 14, 2025 at 7:22 PM
This is a fantastic route for international post docs to perform research in a UK lab. Bristol Biochemistry would be a great home, including our own endosomal-lysosomal lab. royalsociety.org/grants/newto...
Reposted by Peter Cullen
And now onto @carlosantonplagaro.bsky.social from the Pete Cullen lab using BioID to understand the role of Rabs in fate determination in endosomal sorting - great work, Carlos! #UKMT2024
December 16, 2024 at 3:05 PM
And now onto @carlosantonplagaro.bsky.social from the Pete Cullen lab using BioID to understand the role of Rabs in fate determination in endosomal sorting - great work, Carlos! #UKMT2024
Reposted by Peter Cullen
This is an great opportunity. Andrew, his science, and his team are amazing!
Exciting 3-year postdoc opportunity with @carter-lab.bsky.social within a programme aimed at identifying the links between microtubule motors and axonal cargos.
Read more about the role here:
www.nature.com/naturecareer...
Apply by 30 DEC
#ScienceJobs #CambridgeJobs #PostdocJobs #GenomeEngineering
Read more about the role here:
www.nature.com/naturecareer...
Apply by 30 DEC
#ScienceJobs #CambridgeJobs #PostdocJobs #GenomeEngineering
December 12, 2024 at 3:30 PM
This is an great opportunity. Andrew, his science, and his team are amazing!
Reposted by Peter Cullen
Endosome maturation is orchestrated by inside-out proton signaling through a Na+/H+ exchanger and pH-dependent Rab GTPase cycling https://www.biorxiv.org/content/10.1101/2024.12.09.627558v1
December 12, 2024 at 3:32 PM
Endosome maturation is orchestrated by inside-out proton signaling through a Na+/H+ exchanger and pH-dependent Rab GTPase cycling https://www.biorxiv.org/content/10.1101/2024.12.09.627558v1
Reposted by Peter Cullen
Finally my first author preprint at Cullen's lab is out! Amazing collaboration with Kevin and @brettcollins.bsky.social where, by using dedicated proximity proteomics, Biochemistry, crystallography and structural prediction, we show new aspects of human Retromer complex acting as hub for RAB GTPases
Mapping the endosomal proximity proteome reveals Retromer as a hub for RAB GTPase regulation. https://www.biorxiv.org/content/10.1101/2024.11.22.622898v1
Mapping the endosomal proximity proteome reveals Retromer as a hub for RAB GTPase regulation. https://www.biorxiv.org/content/10.1101/2024.11.22.622898v1
Endosomal retrieval and recycling of integral cargo proteins is essential for cell, tissue and organ
www.biorxiv.org
November 24, 2024 at 6:37 PM
Finally my first author preprint at Cullen's lab is out! Amazing collaboration with Kevin and @brettcollins.bsky.social where, by using dedicated proximity proteomics, Biochemistry, crystallography and structural prediction, we show new aspects of human Retromer complex acting as hub for RAB GTPases
Our latest paper with @brettcollins.bsky.social is on bioRxiv. Fantastic work driven by Carlos Anton-Plagaro with unbelievable help from Kai-en Chen in Brett’s lab. A data-rich study with lots of new insight into Retromer, Retriever and potential AD/PD connections. www.biorxiv.org/content/10.1...
Mapping the endosomal proximity proteome reveals Retromer as a hub for RAB GTPase regulation.
Endosomal retrieval and recycling of integral cargo proteins is essential for cell, tissue and organism-level development and homeostasis and is orchestrated through a specialised retrieval sub-domain...
www.biorxiv.org
November 23, 2024 at 1:07 PM
Our latest paper with @brettcollins.bsky.social is on bioRxiv. Fantastic work driven by Carlos Anton-Plagaro with unbelievable help from Kai-en Chen in Brett’s lab. A data-rich study with lots of new insight into Retromer, Retriever and potential AD/PD connections. www.biorxiv.org/content/10.1...
Reposted by Peter Cullen
Very proud to be involved in this work with the Cullen lab. Carlos's proteomics is some of the nicest I've seen! Very carefully controlled proximity labelling reveals a number of novel Rab GTPase activators and regulatory proteins are directly associated with Retromer on the endosome.
Mapping the endosomal proximity proteome reveals Retromer as a hub for RAB GTPase regulation. https://www.biorxiv.org/content/10.1101/2024.11.22.622898v1
Mapping the endosomal proximity proteome reveals Retromer as a hub for RAB GTPase regulation. https://www.biorxiv.org/content/10.1101/2024.11.22.622898v1
Endosomal retrieval and recycling of integral cargo proteins is essential for cell, tissue and organ
www.biorxiv.org
November 23, 2024 at 3:44 AM
Very proud to be involved in this work with the Cullen lab. Carlos's proteomics is some of the nicest I've seen! Very carefully controlled proximity labelling reveals a number of novel Rab GTPase activators and regulatory proteins are directly associated with Retromer on the endosome.