The Hsp70 molecular chaperone system is the front line of defense in maintaining cellular proteostasis. In eukaryotes, ATP/ADP nucleotide exchange in the Hsp70 chaperone cycle is stimulated by Hsp110,...

Calvo et al. describe a new bioluminescent sensor for measuring luminal Ca2+ in a mildly acidic subcompartment of the endo-lysosomal system. This Ca2+ stor

The structural mechanism of a natively isolated GRP94 folding intermediate tethered to a CCDC134-bound translocon is described, revealing how the nascent chain remodels the translocon to control N-glycosylation by OST.

Epithelial cell polarization is essential for many physiological processes, including tissue morphogenesis, nutrient absorption, barrier integrity, and directional secretion. A defining feature of suc...

Integrin-based adhesion complexes serve as primary sites for actomyosin force transmission to the extracellular matrix, providing traction that drives cell mechanical responses including adhesion, mig...

Nature Reviews Molecular Cell Biology, Published online: 20 November 2025; doi:10.1038/s41580-025-00897-2Biomolecular condensates are non-membrane-encapsulated compartments that control various biological processes. Recent studies have revealed that condensates change in response to stimuli and over time. This Review discusses the heterogeneity and composition changes of nuclear and cytoplasmic condensates, their regulation and how the changes affect cellular biochemical reactions.

Li et al. uncover a connection between metabolic cues and mitochondrial protein degradation, showing that specifically leucine stabilizes outer mitochondrial membrane proteins by inhibiting ubiquityla...

Lu et al. show that, under proteotoxic stress, TDP-43 inclusions of skein-like morphology are guided by the chaperone HSP70 and its nucleotide exchange factor BAG3 to induce TDP-43 co-aggregation with...