As a final note, this whole process is highly relevant not only for killing pathogens, but also in autoimmune diseases.

We could also show that sputum of cystic fibrosis patients contains high levels of MPO functionalised NET-like structures.

MPO monomers, a minor fraction of the native MPO pool, can also bind to nucleosomes.

But, unlike dimers, they cannot disassemble the nucleosomes and remain stably associated.

This means that most nucleosomes will be evicted during NETosis, but some are protected by MPO monomers and end up in NETs

Finally, we went back into "real" NETs and saw by #CryoET that (1) NETs are highly complex and contain more than just decondensed chromatin, and (2) that they contain nucleosomes decorated with something that might be MPO.

We see this disorder of the DNA terminus in all our reconstructions when MPO dimers are present, and only then. A strong indication that this is what actually destabilises the nucleosome!

Indeed, only MPO dimers and not monomers can lead to unwrapping of DNA, making it accessible for nucleases:

A tiny difference in the nucleosome structures is that once the second MPO unit is present, there would be a clash with the DNA terminus.

As a consequence, in the presence of MPO dimer the DNA has to locally unwrap and becomes disordered, as visible in this morph:

MPO dimers bind to the nucleosome in an identical way as the monomers we had seen before.

But the second MPO unit contacts the DNA at several positions. And this is important for understanding how it can destabilise the nucleosome.

Okay, great. But what about native MPO? Does it behave similarly as the recombinant one?

Not exactly! Instead, it rapidly disassembles nucleosomes when we reconstitute both, leaving behind only MPO bound to DNA! That was a surprise, since we did not add any other factors such as ATP.

We then reconstituted nucleosomes with a recombinant MPO that resembles a monomeric precursor of native (dimeric) MPO.

By #CryoEM, we saw that MPO stably binds to the acidic patch of the nucleosome, an a binding mode similar to many other nucleosome interactors.

The first thing we found is that MPO associates with nucleosomes in mature NETs.

This interaction is direct and requires intact nucleosomes.

Intriguingly, #myeloperoxidase (MPO) is a key player both for the chromatin decondensation during NETosis and it also decorates mature NETs.

It produces reactive oxygen species and is one of the most important bactericidal enzymes of neutrophils.

During #NETosis, neutrophils decondense their #chromatin by disassembling #nucleosomes. Then, their nucleus breaks down and the cells burst open. The decondensed chomatin then forms the NET.

As #NETs are sticky and functionalised with bactericidal enzymes, they can trap and kill bacteria.

Neutrophils are the master killers of the innate immune response. They can hunt down bacteria and eliminate them using a wide range of mechanisms, including phagocytosis and the release of ROS-generating enzymes including.

And they can form so-called Neutrophil Extracellular Traps (NETs).

2/3 The most striking feature of the structure highly positively charged polybasic stretch on the intracellular side of the membrane.

This stretch could locally change the voltage potential across the membrane and allow Slo1 to open without membrane depolarization!

1/3 Today I can proudly present the first preprint from my project group!

biorxiv.org/content/10.1...

We determined the CryoEM structure of the Slo1 potassium channel in complex with the regulatory subunit γ1 and propose a mechanism of how γ1 can activate Slo1.

Together with Stefan Raunser, I wrote a little review on neurotoxic insecticides acting on ion channels.

We focus on structures and mechanisms and try to reveal similarities in modes of action between chemically diverse compounds. In the end, many act surprisingly similar!

rdcu.be/doHyK