Ming Tommy Tang
@tommytang.bsky.social
Director of bioinformatics at AstraZeneca. subscribe to my youtube channel @chatomics. On my way to helping 1 million people learn bioinformatics. Educator, Biotech, single cell. Also talks about leadership.
tommytang.bio.link
tommytang.bio.link
Priming with DNMT Inhibitors Potentiates PD-1 Immunotherapy by Triggering Viral Mimicry in Relapsed/Refractory NK/T-cell Lymphoma aacrjournals.org/cancerdisco...
November 19, 2025 at 3:15 PM
Priming with DNMT Inhibitors Potentiates PD-1 Immunotherapy by Triggering Viral Mimicry in Relapsed/Refractory NK/T-cell Lymphoma aacrjournals.org/cancerdisco...
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What the heck is an "object" (like a Seurat object) in programming?
If you're in bioinformatics, this term shows up a lot.
Let’s break it down with examples that actually make sense 🧵
What the heck is an "object" (like a Seurat object) in programming?
If you're in bioinformatics, this term shows up a lot.
Let’s break it down with examples that actually make sense 🧵
November 19, 2025 at 2:45 PM
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What the heck is an "object" (like a Seurat object) in programming?
If you're in bioinformatics, this term shows up a lot.
Let’s break it down with examples that actually make sense 🧵
What the heck is an "object" (like a Seurat object) in programming?
If you're in bioinformatics, this term shows up a lot.
Let’s break it down with examples that actually make sense 🧵
Why understanding biology matters in bioinformatics
One big lesson: RNA and protein levels aren’t always correlated. If you don’t know this, you might draw the wrong conclusions. 🧵👇
One big lesson: RNA and protein levels aren’t always correlated. If you don’t know this, you might draw the wrong conclusions. 🧵👇
November 19, 2025 at 2:15 PM
Why understanding biology matters in bioinformatics
One big lesson: RNA and protein levels aren’t always correlated. If you don’t know this, you might draw the wrong conclusions. 🧵👇
One big lesson: RNA and protein levels aren’t always correlated. If you don’t know this, you might draw the wrong conclusions. 🧵👇
it is more fragile than you think
November 19, 2025 at 1:24 AM
it is more fragile than you think
Comprehensive tumor-immune profiling reveals mediators of paradoxical immune sensitivity in sarcomatoid renal cell carcinoma www.cell.com/cancer-cell...
November 18, 2025 at 3:15 PM
Comprehensive tumor-immune profiling reveals mediators of paradoxical immune sensitivity in sarcomatoid renal cell carcinoma www.cell.com/cancer-cell...
🧵 RNA-seq Normalization: What You Need to Know
November 18, 2025 at 2:45 PM
🧵 RNA-seq Normalization: What You Need to Know
The Secret to Recreating Any Bioinformatics Figure (It’s easier than You Think). The other day, I was on Bioinformatics subReddit and saw someone ask how to generate the figure below
November 18, 2025 at 2:15 PM
The Secret to Recreating Any Bioinformatics Figure (It’s easier than You Think). The other day, I was on Bioinformatics subReddit and saw someone ask how to generate the figure below
Paneth-like transition drives resistance to dual targeting of KRAS and EGFR in colorectal cancer www.cell.com/cancer-cell...
November 17, 2025 at 3:15 PM
Paneth-like transition drives resistance to dual targeting of KRAS and EGFR in colorectal cancer www.cell.com/cancer-cell...
Want to master bioinformatics data visualization? Learn ggplot2! 🧵👇
November 17, 2025 at 2:45 PM
Want to master bioinformatics data visualization? Learn ggplot2! 🧵👇
Thread: Multi-omics sounds cool—until you actually try it. Here's are the nuances.
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You’ve got RNA-seq.
Methylation.
Proteomics.
Time to “integrate” the data.
But how? And why?
Let’s break it down.
1/
You’ve got RNA-seq.
Methylation.
Proteomics.
Time to “integrate” the data.
But how? And why?
Let’s break it down.
November 17, 2025 at 2:15 PM
Thread: Multi-omics sounds cool—until you actually try it. Here's are the nuances.
1/
You’ve got RNA-seq.
Methylation.
Proteomics.
Time to “integrate” the data.
But how? And why?
Let’s break it down.
1/
You’ve got RNA-seq.
Methylation.
Proteomics.
Time to “integrate” the data.
But how? And why?
Let’s break it down.
Making a heatmap is an essential skill for a bioinformatician. But you probably do not understand heatmap. 7 reading resources to understand heatmap! 🧵
November 16, 2025 at 2:45 PM
Making a heatmap is an essential skill for a bioinformatician. But you probably do not understand heatmap. 7 reading resources to understand heatmap! 🧵
1/ "How can I start learning bioinformatics?" As a former wet lab scientist, I hear this question a lot. Here's my journey—and lessons learned.
November 16, 2025 at 2:15 PM
1/ "How can I start learning bioinformatics?" As a former wet lab scientist, I hear this question a lot. Here's my journey—and lessons learned.
Extrachromosomal DNA associates with nuclear condensates and reorganizes chromatin structures to enhance oncogenic transcription www.cell.com/cancer-cell...
November 15, 2025 at 3:15 PM
Extrachromosomal DNA associates with nuclear condensates and reorganizes chromatin structures to enhance oncogenic transcription www.cell.com/cancer-cell...
An introduction to Python for R users occasionaldivergences.com/posts/pytho...
November 15, 2025 at 2:45 PM
An introduction to Python for R users occasionaldivergences.com/posts/pytho...
1/ Exploratory Data Analysis (EDA) is the first step in any data analysis journey. When working with RNA-seq data, one of the most commonly used techniques is Principal Component Analysis (PCA). But what exactly is PCA, and why does it matter? Let’s break it down. 🧵👇
November 15, 2025 at 2:15 PM
1/ Exploratory Data Analysis (EDA) is the first step in any data analysis journey. When working with RNA-seq data, one of the most commonly used techniques is Principal Component Analysis (PCA). But what exactly is PCA, and why does it matter? Let’s break it down. 🧵👇
8 Resources to study Transcription factor binding, enhancers and histone modification distribution
1. ENCODE www.encodeproject.org/
1. ENCODE www.encodeproject.org/
November 14, 2025 at 3:15 PM
8 Resources to study Transcription factor binding, enhancers and histone modification distribution
1. ENCODE www.encodeproject.org/
1. ENCODE www.encodeproject.org/
1/ You think RNA equals protein?
Not always.
If you're doing bioinformatics without understanding biology, you're flying blind. 🧵
Not always.
If you're doing bioinformatics without understanding biology, you're flying blind. 🧵
November 14, 2025 at 2:15 PM
1/ You think RNA equals protein?
Not always.
If you're doing bioinformatics without understanding biology, you're flying blind. 🧵
Not always.
If you're doing bioinformatics without understanding biology, you're flying blind. 🧵
1/ Everyone’s building deep learning pipelines.
But no one’s asking:
Does this data even make sense?
Let’s talk about the basics. 🧵
But no one’s asking:
Does this data even make sense?
Let’s talk about the basics. 🧵
November 13, 2025 at 3:15 PM
1/ Everyone’s building deep learning pipelines.
But no one’s asking:
Does this data even make sense?
Let’s talk about the basics. 🧵
But no one’s asking:
Does this data even make sense?
Let’s talk about the basics. 🧵
1/ You copy-paste text.
You load a file.
Nothing looks wrong—until your code breaks because of invisible whitespace.
Here’s how to fix it 🧵
You load a file.
Nothing looks wrong—until your code breaks because of invisible whitespace.
Here’s how to fix it 🧵
November 13, 2025 at 2:45 PM
1/ You copy-paste text.
You load a file.
Nothing looks wrong—until your code breaks because of invisible whitespace.
Here’s how to fix it 🧵
You load a file.
Nothing looks wrong—until your code breaks because of invisible whitespace.
Here’s how to fix it 🧵
GSEA is one of the highly cited methods (> 50K! ) for gene set enrichment analysis. Do you really understand it?
November 13, 2025 at 2:15 PM
GSEA is one of the highly cited methods (> 50K! ) for gene set enrichment analysis. Do you really understand it?
1/ Many bioinformatics students don’t know much about NGS pre-processing. But trust me, understanding the raw data is essential. Here’s why.
November 12, 2025 at 3:15 PM
1/ Many bioinformatics students don’t know much about NGS pre-processing. But trust me, understanding the raw data is essential. Here’s why.
1/ You’ve got dozens of VCFs from a sequencing project.
But here’s the problem:
Which variants are shared across multiple samples? 🧵
But here’s the problem:
Which variants are shared across multiple samples? 🧵
November 12, 2025 at 2:45 PM
1/ You’ve got dozens of VCFs from a sequencing project.
But here’s the problem:
Which variants are shared across multiple samples? 🧵
But here’s the problem:
Which variants are shared across multiple samples? 🧵
1/ AI won’t save sloppy science.
Before you dive into deep learning, master your foundations.
Here’s why basic bioinformatics still rules 🧵
Before you dive into deep learning, master your foundations.
Here’s why basic bioinformatics still rules 🧵
November 12, 2025 at 2:15 PM
1/ AI won’t save sloppy science.
Before you dive into deep learning, master your foundations.
Here’s why basic bioinformatics still rules 🧵
Before you dive into deep learning, master your foundations.
Here’s why basic bioinformatics still rules 🧵
Mutation of CMTR2 in Lung Adenocarcinoma Alters RNA Alternative Splicing and Reveals Therapeutic Vulnerabilities www.nature.com/articles/s4...
November 11, 2025 at 3:15 PM
Mutation of CMTR2 in Lung Adenocarcinoma Alters RNA Alternative Splicing and Reveals Therapeutic Vulnerabilities www.nature.com/articles/s4...
1/ Working with big data in R?
Your wrangling just got a massive upgrade.
duckplyr is now in the tidyverse—and it’s fast. Really fast. 🧵
Your wrangling just got a massive upgrade.
duckplyr is now in the tidyverse—and it’s fast. Really fast. 🧵
November 11, 2025 at 2:45 PM
1/ Working with big data in R?
Your wrangling just got a massive upgrade.
duckplyr is now in the tidyverse—and it’s fast. Really fast. 🧵
Your wrangling just got a massive upgrade.
duckplyr is now in the tidyverse—and it’s fast. Really fast. 🧵