Thom Hallmark, PhD
@tjhallmark.bsky.social
Postdoc studying novel pAgos @WUR Netherlands 🇳🇱
PhD, Biochemistry studying CRISPR-Cas12a2 @USU
BSc, Biochemistry @BYU-idaho
PhD, Biochemistry studying CRISPR-Cas12a2 @USU
BSc, Biochemistry @BYU-idaho
Honestly, this is a life dream
October 7, 2025 at 11:42 AM
Honestly, this is a life dream
This is so sad. A loss...
March 20, 2025 at 7:58 PM
This is so sad. A loss...
In Exedos, these venomous snakes start biting people, and all they had to do to be healed of the snake bites was look at this brass snake on a stick. Same story, different details.
February 22, 2025 at 6:33 PM
In Exedos, these venomous snakes start biting people, and all they had to do to be healed of the snake bites was look at this brass snake on a stick. Same story, different details.
Wow, that's a pretty structure
January 1, 2025 at 6:08 AM
Wow, that's a pretty structure
These are some fun traditions :)
January 1, 2025 at 5:59 AM
These are some fun traditions :)
Someone should tell academics...
December 19, 2024 at 3:46 PM
Someone should tell academics...
Very insightful
December 18, 2024 at 6:02 PM
Very insightful
Reading a recursive method section with "we performed the method as described in (citation)" going back 10 years
December 11, 2024 at 4:55 AM
Reading a recursive method section with "we performed the method as described in (citation)" going back 10 years
This dissertation describes my work characterizing Cas12a2 to uncover the fundamental principles governing RNA targeted cell killing.
If your interested in targeted cell killing, follow me. There are several cool stories in the works from the Jackson, Beisel and Liu labs (not on 🦋)
If your interested in targeted cell killing, follow me. There are several cool stories in the works from the Jackson, Beisel and Liu labs (not on 🦋)
December 11, 2024 at 4:48 AM
This dissertation describes my work characterizing Cas12a2 to uncover the fundamental principles governing RNA targeted cell killing.
If your interested in targeted cell killing, follow me. There are several cool stories in the works from the Jackson, Beisel and Liu labs (not on 🦋)
If your interested in targeted cell killing, follow me. There are several cool stories in the works from the Jackson, Beisel and Liu labs (not on 🦋)
We think Cas12a2 could be repurposed to selectively kill cells based on specific RNA targets (eg. Pathogens, cancer, autoimmune cells, &c.) while leaving healthy cells unharmed. But before using Cas12a2 for these applications we needed to understand the basics of its activity.
December 11, 2024 at 4:41 AM
We think Cas12a2 could be repurposed to selectively kill cells based on specific RNA targets (eg. Pathogens, cancer, autoimmune cells, &c.) while leaving healthy cells unharmed. But before using Cas12a2 for these applications we needed to understand the basics of its activity.
Cas12a2 is an RNA guided nuclease like Cas9, but instead of targeting dsDNA, Cas12a2 recognizes RNA targets. Target binding activates a collateral nuclease activity that cleaves RNA, ssDNA, and dsDNA! This shreds all the nucleic acid in the cell, causing growth arrest and cell death.
December 11, 2024 at 4:37 AM
Cas12a2 is an RNA guided nuclease like Cas9, but instead of targeting dsDNA, Cas12a2 recognizes RNA targets. Target binding activates a collateral nuclease activity that cleaves RNA, ssDNA, and dsDNA! This shreds all the nucleic acid in the cell, causing growth arrest and cell death.
Watch out for updates as the rest of my data becomes available!
December 11, 2024 at 4:30 AM
Watch out for updates as the rest of my data becomes available!
Contents:
Ch.1 review regulating mechanisms Of CRISPR systems
Ch.2 Detailed methods (submitted)
Ch.3 Cas12a2 activity (www.nature.com/articles/s41...)
Ch.4 Cas12a2 structure (www.nature.com/articles/s41...)
Ch.5 Substrate preference (submitted)
Ch.6 Activation mechanism of Cas12a2 (preparation)
Ch.1 review regulating mechanisms Of CRISPR systems
Ch.2 Detailed methods (submitted)
Ch.3 Cas12a2 activity (www.nature.com/articles/s41...)
Ch.4 Cas12a2 structure (www.nature.com/articles/s41...)
Ch.5 Substrate preference (submitted)
Ch.6 Activation mechanism of Cas12a2 (preparation)
Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA - Nature
RNA targeting by the Sulfuricurvum type V single-effector nuclease SuCas12a2 drives abortive infection through non-specific cleavage of double-stranded DNA—after recognition of an RNA target through a...
www.nature.com
December 11, 2024 at 4:29 AM
Contents:
Ch.1 review regulating mechanisms Of CRISPR systems
Ch.2 Detailed methods (submitted)
Ch.3 Cas12a2 activity (www.nature.com/articles/s41...)
Ch.4 Cas12a2 structure (www.nature.com/articles/s41...)
Ch.5 Substrate preference (submitted)
Ch.6 Activation mechanism of Cas12a2 (preparation)
Ch.1 review regulating mechanisms Of CRISPR systems
Ch.2 Detailed methods (submitted)
Ch.3 Cas12a2 activity (www.nature.com/articles/s41...)
Ch.4 Cas12a2 structure (www.nature.com/articles/s41...)
Ch.5 Substrate preference (submitted)
Ch.6 Activation mechanism of Cas12a2 (preparation)
My PhD research focused on studying new bacterial immune systems, specifically, new CRISPR systems. Most people think of CRISPR as a genome editor, but it started as a way for bacteria to defend against viruses.
December 11, 2024 at 4:20 AM
My PhD research focused on studying new bacterial immune systems, specifically, new CRISPR systems. Most people think of CRISPR as a genome editor, but it started as a way for bacteria to defend against viruses.
Because my dissertation contains some exciting data yet to be published it is currently under embargo (sorry no downloads 'til 2029), I want to give a quick overview of my work.
December 11, 2024 at 4:15 AM
Because my dissertation contains some exciting data yet to be published it is currently under embargo (sorry no downloads 'til 2029), I want to give a quick overview of my work.
Especially this time of year, on the oregon coast, you can never be sure. Always happy for sunny days
November 29, 2024 at 4:14 AM
Especially this time of year, on the oregon coast, you can never be sure. Always happy for sunny days
Common sense is woefully uncommon :/ hopefully we can keep schools around at least until it is...
November 28, 2024 at 5:45 AM
Common sense is woefully uncommon :/ hopefully we can keep schools around at least until it is...
The first two: bottom of the first overlaps with the top of the second and rescaled?
November 22, 2024 at 3:16 AM
The first two: bottom of the first overlaps with the top of the second and rescaled?
I have really loved the starter packs. The other place was so hard to find people to connect with!
November 18, 2024 at 6:37 PM
I have really loved the starter packs. The other place was so hard to find people to connect with!