B Thuronyi (they/them)
@thuronyi.bsky.social
Synthetic biologist, V. natriegens booster, Sheets torturer & asst prof Chem @ Williams College
Marketing is very important! The inertia of previously established methods -- and even previously established GAPS, i.e. "I know that's not doable so I'm not interested in trying" -- is enormous...
November 23, 2025 at 6:56 PM
Marketing is very important! The inertia of previously established methods -- and even previously established GAPS, i.e. "I know that's not doable so I'm not interested in trying" -- is enormous...
Thank you for bringing this energy
November 22, 2025 at 12:31 AM
Thank you for bringing this energy
Hiii!! Thank you! It's just B now! I'm in the last year before tenure submission and sweating bullets but it'll probably all be ok......
Hope you're doing good too!
Hope you're doing good too!
November 22, 2025 at 12:10 AM
Hiii!! Thank you! It's just B now! I'm in the last year before tenure submission and sweating bullets but it'll probably all be ok......
Hope you're doing good too!
Hope you're doing good too!
Woohoo! Let me know how it goes! If you join the Slack we can help with onboarding
November 21, 2025 at 10:14 PM
Woohoo! Let me know how it goes! If you join the Slack we can help with onboarding
Reposted by B Thuronyi (they/them)
this looks like a dream come true for my lab. Can't wait to try it out!
November 21, 2025 at 9:52 PM
this looks like a dream come true for my lab. Can't wait to try it out!
Would love to have some boosts to help with visibility for this project as I start from scratch here on Bluesky... great to see some old friends like @atinygreencell.bsky.social here and I hope to rediscover more!
November 21, 2025 at 7:40 PM
Would love to have some boosts to help with visibility for this project as I start from scratch here on Bluesky... great to see some old friends like @atinygreencell.bsky.social here and I hope to rediscover more!
Let's make it easier to document what we're doing so we can learn from our own and others' experiences with cloning and all the many operations and decisions involved in handling DNA.
clonecoordinate.org
clonecoordinate.org
CloneCoordinate | Free, open source lab-scale DNA foundry software for collaborative cloning
clonecoordinate.org
November 21, 2025 at 7:40 PM
Let's make it easier to document what we're doing so we can learn from our own and others' experiences with cloning and all the many operations and decisions involved in handling DNA.
clonecoordinate.org
clonecoordinate.org
Building DNA that's never existed before is still central to synbio and lots of life sciences. No matter how much you outsource, there's still tons to keep track of and manage, and lots of projects still get hung up on cloning!
November 21, 2025 at 7:40 PM
Building DNA that's never existed before is still central to synbio and lots of life sciences. No matter how much you outsource, there's still tons to keep track of and manage, and lots of projects still get hung up on cloning!
Yes, DNA synthesis is getting cheaper and easier and cloning as a fee-for-service is increasingly common. Yes, @plasmidsaurus.bsky.social is making it easier than ever to verify your materials and find out how your assemblies went wrong. (thanks @dna.bsky.social!)
BUT...
BUT...
November 21, 2025 at 7:40 PM
Yes, DNA synthesis is getting cheaper and easier and cloning as a fee-for-service is increasingly common. Yes, @plasmidsaurus.bsky.social is making it easier than ever to verify your materials and find out how your assemblies went wrong. (thanks @dna.bsky.social!)
BUT...
BUT...
Cloning at scale at a primarily, or exclusively, undergrad institution is very possible with the right tools. Now that we have those, we want to share them with everyone! CC let us work as a collaborative team, even across generations of students.
November 21, 2025 at 7:40 PM
Cloning at scale at a primarily, or exclusively, undergrad institution is very possible with the right tools. Now that we have those, we want to share them with everyone! CC let us work as a collaborative team, even across generations of students.
We've cloned four hundred or so constructs over the last 5 years (a bunch of them recently published with @fritzlab.bsky.social as part of the Vnat Collection) with an undergrad-only team *while* we built CC from scratch...
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Expanding genetic engineering capabilities in Vibrio natriegens with the Vnat Collection
Abstract. Vibrio natriegens, with its exceptionally fast growth rate, has great promise as a revolutionary chassis for synthetic biology, yet the realizati
academic.oup.com
November 21, 2025 at 7:40 PM
We've cloned four hundred or so constructs over the last 5 years (a bunch of them recently published with @fritzlab.bsky.social as part of the Vnat Collection) with an undergrad-only team *while* we built CC from scratch...
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
CC is a framework for recording the granular details of DNA assembly (in familiar spreadsheet format) so they can be part of publishing -- joining other neglected elements like, uh, construct sequences?? (as I wrote w/@erika-alden.bsky.social and Jeff Barrick):
journals.plos.org/plosbiology/...
journals.plos.org/plosbiology/...
No assembly required: Time for stronger, simpler publishing standards for DNA sequences
Describing how DNA constructs were assembled is no longer necessary when it is possible to fully sequence the final results to validate them. In this Perspective, the authors suggest that outdated met...
journals.plos.org
November 21, 2025 at 7:40 PM
CC is a framework for recording the granular details of DNA assembly (in familiar spreadsheet format) so they can be part of publishing -- joining other neglected elements like, uh, construct sequences?? (as I wrote w/@erika-alden.bsky.social and Jeff Barrick):
journals.plos.org/plosbiology/...
journals.plos.org/plosbiology/...
If you're a responsible researcher who wants their carefully collected data to benefit the broadest possible community, why not document the details of how you MAKE all those constructs you're testing so you can share that valuable experience with others?
November 21, 2025 at 7:40 PM
If you're a responsible researcher who wants their carefully collected data to benefit the broadest possible community, why not document the details of how you MAKE all those constructs you're testing so you can share that valuable experience with others?
If you've thought about working cloning into undergrad research or summer projects or teaching labs or your iGEM @igemhq.bsky.social team but it feels like an uphill battle getting everyone up to speed, keeping them on the same page, and having them work productively as a unit – consider CC!
November 21, 2025 at 7:40 PM
If you've thought about working cloning into undergrad research or summer projects or teaching labs or your iGEM @igemhq.bsky.social team but it feels like an uphill battle getting everyone up to speed, keeping them on the same page, and having them work productively as a unit – consider CC!
If you're in the early stages of starting a lab (hang in there!!) this is a great time to start off on the right foot with a highly organized system that will make sure as little as possible slips through the cracks and take a lot of cognitive load off your team.
November 21, 2025 at 7:40 PM
If you're in the early stages of starting a lab (hang in there!!) this is a great time to start off on the right foot with a highly organized system that will make sure as little as possible slips through the cracks and take a lot of cognitive load off your team.
We all have our takes on what makes cloning work well or poorly. We might all be right: every lab's techniques and constructs are different, so it seems like the best way to go is use data about YOUR cloning to make decisions about how to do YOUR cloning.
November 21, 2025 at 7:40 PM
We all have our takes on what makes cloning work well or poorly. We might all be right: every lab's techniques and constructs are different, so it seems like the best way to go is use data about YOUR cloning to make decisions about how to do YOUR cloning.
Even with my years of training I still couldn't keep the progress and to-do list for more than a dozen or so constructs organized at once. Now I just outsource all that to a computer and focus on the science.
November 21, 2025 at 7:40 PM
Even with my years of training I still couldn't keep the progress and to-do list for more than a dozen or so constructs organized at once. Now I just outsource all that to a computer and focus on the science.
CloneCoordinate is the cloning management tool I wish I'd had during my postdoc in the Liu lab -- would've made the 200-odd plasmids I reported, and the hundreds of others I dropped along the way or couldn't finish, a lot easier to manage.
November 21, 2025 at 7:40 PM
CloneCoordinate is the cloning management tool I wish I'd had during my postdoc in the Liu lab -- would've made the 200-odd plasmids I reported, and the hundreds of others I dropped along the way or couldn't finish, a lot easier to manage.
If you're an experienced cloner wanting to track your dozens of constructs-in-progress – or manage your thousands of physical samples – or start accumulating data to back up (or overturn!) all the hunches about how to do it best that you've developed over the years – try CC!
November 21, 2025 at 7:40 PM
If you're an experienced cloner wanting to track your dozens of constructs-in-progress – or manage your thousands of physical samples – or start accumulating data to back up (or overturn!) all the hunches about how to do it best that you've developed over the years – try CC!
I have been teasing this paper for years now and am so excited it's in a place to broadly share.
Besides the paper itself, you can find out more about CloneCoordinate's features and mission at clonecoordinate.org. But here's a quick pitch! 🧵
Besides the paper itself, you can find out more about CloneCoordinate's features and mission at clonecoordinate.org. But here's a quick pitch! 🧵
November 21, 2025 at 7:40 PM
I have been teasing this paper for years now and am so excited it's in a place to broadly share.
Besides the paper itself, you can find out more about CloneCoordinate's features and mission at clonecoordinate.org. But here's a quick pitch! 🧵
Besides the paper itself, you can find out more about CloneCoordinate's features and mission at clonecoordinate.org. But here's a quick pitch! 🧵
(This thread is crossposted from... that other place... where I returned after years away just to spread the word, and am happy to see many folks over HERE who I've really missed seeing!)
November 21, 2025 at 7:40 PM
(This thread is crossposted from... that other place... where I returned after years away just to spread the word, and am happy to see many folks over HERE who I've really missed seeing!)