Structura Biotechnology
@structurabio.bsky.social
Algorithms, Software and Systems for Cryo-EM. Makers of CryoSPARC.
Pinned
🚀 Introducing fully automated data processing for repeat-target #cryoEM.
Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.
Preprint: www.biorxiv.org/content/10.1...
Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.
Preprint: www.biorxiv.org/content/10.1...
🚀 Hiring: Cryo-EM Application Scientist, Marketing!
Develop and deliver high-impact scientific marketing content, illustrate the value of new #CryoSPARC features and #cryoEM advances, and help scientists understand how our tools can help solve their problems.
structura.bio/careers/appl...
Develop and deliver high-impact scientific marketing content, illustrate the value of new #CryoSPARC features and #cryoEM advances, and help scientists understand how our tools can help solve their problems.
structura.bio/careers/appl...
Structura Bio | Structura Bio
structura.bio
November 20, 2025 at 9:00 PM
🚀 Hiring: Cryo-EM Application Scientist, Marketing!
Develop and deliver high-impact scientific marketing content, illustrate the value of new #CryoSPARC features and #cryoEM advances, and help scientists understand how our tools can help solve their problems.
structura.bio/careers/appl...
Develop and deliver high-impact scientific marketing content, illustrate the value of new #CryoSPARC features and #cryoEM advances, and help scientists understand how our tools can help solve their problems.
structura.bio/careers/appl...
Reposted by Structura Biotechnology
Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem
CAHRA 2025: Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms
A community-wide data processing challenge for cryo-electron microscopy.
heterogeneity.notion.site
November 7, 2025 at 10:45 PM
Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem
Reposted by Structura Biotechnology
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
October 30, 2025 at 3:21 AM
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look?
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
October 30, 2025 at 1:33 PM
On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look?
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
🚀 Introducing fully automated data processing for repeat-target #cryoEM.
Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.
Preprint: www.biorxiv.org/content/10.1...
Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.
Preprint: www.biorxiv.org/content/10.1...
October 20, 2025 at 2:32 PM
🚀 Introducing fully automated data processing for repeat-target #cryoEM.
Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.
Preprint: www.biorxiv.org/content/10.1...
Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.
Preprint: www.biorxiv.org/content/10.1...
Reposted by Structura Biotechnology
End-to-end automation of repeat-target cryo-EM structure determination in CryoSPARC https://www.biorxiv.org/content/10.1101/2025.10.17.682689v1
October 20, 2025 at 1:47 PM
End-to-end automation of repeat-target cryo-EM structure determination in CryoSPARC https://www.biorxiv.org/content/10.1101/2025.10.17.682689v1
Reposted by Structura Biotechnology
Helical Reconstruction of Amyloids in cryoSPARC?
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...
October 5, 2025 at 4:16 AM
Helical Reconstruction of Amyloids in cryoSPARC?
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...
Reposted by Structura Biotechnology
1/14
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Evolutionary analysis reveals the origin of sodium coupling in glutamate transporters - Nature Structural & Molecular Biology
Reddy et al. used ancestral protein reconstruction, cryo-electron microscopy and functional assays to elucidate how a secondary active transporter evolved to harness the energy of sodium gradients to ...
go.nature.com
September 12, 2025 at 12:08 AM
1/14
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Reposted by Structura Biotechnology
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 13, 2025 at 12:05 AM
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...
September 10, 2025 at 3:46 PM
Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...
Reposted by Structura Biotechnology
Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️
August 30, 2025 at 2:11 PM
Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️
Reposted by Structura Biotechnology
🧪 Skip the trial and error for structure determination of soluble proteins. Join the #webinar 10/8 for a gene-to-structure case study of CYP3A4, a 57 kDa cytochrome P450 monooxygenase, solved to 3.1 Å by #cryoEM.
nimgs.zoom.us/webinar/regi...
nimgs.zoom.us/webinar/regi...
Welcome! You are invited to join a webinar: Soluble Proteins in Focus: Preparing for Cryo-EM Success. After registering, you will receive a confirmation email about joining the webinar.
Are you using cryo-EM to look at small soluble proteins?
Join Dr. Claudio Catalano, a Senior Scientist at NanoImaging Services, to get expert advice on how to set your project up for success. He wil...
nimgs.zoom.us
August 27, 2025 at 6:12 PM
🧪 Skip the trial and error for structure determination of soluble proteins. Join the #webinar 10/8 for a gene-to-structure case study of CYP3A4, a 57 kDa cytochrome P450 monooxygenase, solved to 3.1 Å by #cryoEM.
nimgs.zoom.us/webinar/regi...
nimgs.zoom.us/webinar/regi...
Reposted by Structura Biotechnology
Exciting work from the Franklin, @ox.ac.uk, and @diamondlightsource.bsky.social has led to a new method for imaging small proteins (<50 kDa) using cryoEM. By using bifunctional, bispecific nanobody scaffolds, the team have successfully solved the smallest protein structure to date (14 kDa).
August 18, 2025 at 3:27 PM
Exciting work from the Franklin, @ox.ac.uk, and @diamondlightsource.bsky.social has led to a new method for imaging small proteins (<50 kDa) using cryoEM. By using bifunctional, bispecific nanobody scaffolds, the team have successfully solved the smallest protein structure to date (14 kDa).
Reposted by Structura Biotechnology
Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets!
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
Covalently constrained ‘Di-Gembodies’ enable parallel structure solutions by cryo-EM - Nature Chemical Biology
Disulfide-based dimerization of modified identical and heterologous nanobody scaffolds enables higher-order assembly for high-resolution cryo-electron microscopy structure determination that is widely...
www.nature.com
August 15, 2025 at 10:00 AM
Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets!
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
Reposted by Structura Biotechnology
Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
High-throughput investigation of cyclin docking interactions reveals the complexity of motif binding determinants - Nature Communications
Many protein–protein interactions depend on Short Linear Motifs (SLiMs). In this study, the authors use large-scale binding assays, deep mutational scanning, and structural analysis to map SLiMs recog...
www.nature.com
August 15, 2025 at 9:26 PM
Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Flexibility can be frustrating when processing a #cryoEM dataset. If your target has a wobbly domain, check out our new case study on FaNaC1 for continuous heterogeneity tips in #CryoSPARC!
guide.cryosparc.com/processing-d...
guide.cryosparc.com/processing-d...
August 5, 2025 at 1:46 PM
Flexibility can be frustrating when processing a #cryoEM dataset. If your target has a wobbly domain, check out our new case study on FaNaC1 for continuous heterogeneity tips in #CryoSPARC!
guide.cryosparc.com/processing-d...
guide.cryosparc.com/processing-d...
Reposted by Structura Biotechnology
Get hands-on training on #cryofreezing, #DataAcquisition and more at EMBO Practical Course "Cryo electron microscopy and 3D image processing" in New Delhi, IN, 21 Feb–3 Mar 2026
Registration by 15 Sep
Abstract submission by 30 Sep
meetings.embo.org/event/26-cem...
#cryoEM #EMBOCryo3D #EMBOevents 🧪
Registration by 15 Sep
Abstract submission by 30 Sep
meetings.embo.org/event/26-cem...
#cryoEM #EMBOCryo3D #EMBOevents 🧪
Cryo electron microscopy and 3D image processing (CEM3DIP)
This EMBO Practical Course is for participants who are interested in analysis of macromolecular structures in vitro and in situ using cryo-electron microscopy single particle reconstruction and t…
meetings.embo.org
July 29, 2025 at 9:50 AM
Get hands-on training on #cryofreezing, #DataAcquisition and more at EMBO Practical Course "Cryo electron microscopy and 3D image processing" in New Delhi, IN, 21 Feb–3 Mar 2026
Registration by 15 Sep
Abstract submission by 30 Sep
meetings.embo.org/event/26-cem...
#cryoEM #EMBOCryo3D #EMBOevents 🧪
Registration by 15 Sep
Abstract submission by 30 Sep
meetings.embo.org/event/26-cem...
#cryoEM #EMBOCryo3D #EMBOevents 🧪
Reposted by Structura Biotechnology
That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
biorxiv.org/content/10.110
biorxiv.org/content/10.110
biorxiv.org
July 23, 2025 at 9:43 PM
That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
biorxiv.org/content/10.110
biorxiv.org/content/10.110
When particles have orientation bias, the resulting maps are often streaked along the missing directions. In this case study, we cover why orientation bias produces anisotropic #cryoEM maps and how you may be able to recover from it!
guide.cryosparc.com/processing-d...
guide.cryosparc.com/processing-d...
July 23, 2025 at 12:29 PM
When particles have orientation bias, the resulting maps are often streaked along the missing directions. In this case study, we cover why orientation bias produces anisotropic #cryoEM maps and how you may be able to recover from it!
guide.cryosparc.com/processing-d...
guide.cryosparc.com/processing-d...
Link here! guide.cryosparc.com/processing-d...
July 14, 2025 at 5:43 PM
Link here! guide.cryosparc.com/processing-d...
Working with symmetric or pseudosymmetric particles in #cryoEM?
This TRPV5 and CaM case study includes practical considerations for using symmetry relaxation and 3D classification when analyzing a pseudosymmetric particle in #CryoSPARC!
This TRPV5 and CaM case study includes practical considerations for using symmetry relaxation and 3D classification when analyzing a pseudosymmetric particle in #CryoSPARC!
July 14, 2025 at 12:48 PM
Working with symmetric or pseudosymmetric particles in #cryoEM?
This TRPV5 and CaM case study includes practical considerations for using symmetry relaxation and 3D classification when analyzing a pseudosymmetric particle in #CryoSPARC!
This TRPV5 and CaM case study includes practical considerations for using symmetry relaxation and 3D classification when analyzing a pseudosymmetric particle in #CryoSPARC!
Reposted by Structura Biotechnology
When I arrived to NYC as a postdoc, NYSBDG was my first local meeting, and I was blown way by the world-class quality of the science.
It's a great opportunity to meet the local #structbio & #cryoem community, and it's coming up in two weeks, so register now at the eventbrite link below!
It's a great opportunity to meet the local #structbio & #cryoem community, and it's coming up in two weeks, so register now at the eventbrite link below!
July 7, 2025 at 2:13 PM
When I arrived to NYC as a postdoc, NYSBDG was my first local meeting, and I was blown way by the world-class quality of the science.
It's a great opportunity to meet the local #structbio & #cryoem community, and it's coming up in two weeks, so register now at the eventbrite link below!
It's a great opportunity to meet the local #structbio & #cryoem community, and it's coming up in two weeks, so register now at the eventbrite link below!
Looking for a deep dive into 2D classification in #cryoEM?
These recent guide pages cover practical aspects & parameter choices when running these jobs in #CryoSPARC, along with the theory behind expectation maximization: guide.cryosparc.com/processing-d... and guide.cryosparc.com/expectation-...
These recent guide pages cover practical aspects & parameter choices when running these jobs in #CryoSPARC, along with the theory behind expectation maximization: guide.cryosparc.com/processing-d... and guide.cryosparc.com/expectation-...
June 24, 2025 at 2:44 PM
Looking for a deep dive into 2D classification in #cryoEM?
These recent guide pages cover practical aspects & parameter choices when running these jobs in #CryoSPARC, along with the theory behind expectation maximization: guide.cryosparc.com/processing-d... and guide.cryosparc.com/expectation-...
These recent guide pages cover practical aspects & parameter choices when running these jobs in #CryoSPARC, along with the theory behind expectation maximization: guide.cryosparc.com/processing-d... and guide.cryosparc.com/expectation-...
Reposted by Structura Biotechnology
1/x New story - the structure of the Vault cap, at 2.3Å! Matching a beautiful preprint from @sjorsscheres.bsky.social, we show that the cap of the vault particle has C13 symmetry, in contrast to the 39-fold symmetry of the body. Study lead by talented postdoc @huanli00.bsky.social from my group! ⬇️
June 12, 2025 at 1:44 PM
1/x New story - the structure of the Vault cap, at 2.3Å! Matching a beautiful preprint from @sjorsscheres.bsky.social, we show that the cap of the vault particle has C13 symmetry, in contrast to the 39-fold symmetry of the body. Study lead by talented postdoc @huanli00.bsky.social from my group! ⬇️
Reposted by Structura Biotechnology
Thank you to all the presenters and scientists from around the country who attended the LSI's 2025 #CryoEM Workshop and made the event such a successful hands-on learning experience!
Thanks also to our event sponsors, SubAngstrom, @globusorg.bsky.social, @mitegen.com and @structurabio.bsky.social
Thanks also to our event sponsors, SubAngstrom, @globusorg.bsky.social, @mitegen.com and @structurabio.bsky.social
June 9, 2025 at 5:56 PM
Thank you to all the presenters and scientists from around the country who attended the LSI's 2025 #CryoEM Workshop and made the event such a successful hands-on learning experience!
Thanks also to our event sponsors, SubAngstrom, @globusorg.bsky.social, @mitegen.com and @structurabio.bsky.social
Thanks also to our event sponsors, SubAngstrom, @globusorg.bsky.social, @mitegen.com and @structurabio.bsky.social