RobertoFeuda
@robertofeuda.bsky.social
Associate Professor at the Univerisity of Bologna. Past Maynooth, Oxford, Caltech, Bristol, Leicester and URF Webpage: https://sites.google.com/view/feudalab/home?authuser=0
This has been a truly collaborative journey with many collaborators (e.g. @jordisolana.bsky.social other are not on bluesky) but I want to highlight @cliftonlewis.bsky.social , who carried out an enormous amount of the work and drove much of the analysis and interpretation.
October 30, 2025 at 9:42 AM
This has been a truly collaborative journey with many collaborators (e.g. @jordisolana.bsky.social other are not on bluesky) but I want to highlight @cliftonlewis.bsky.social , who carried out an enormous amount of the work and drove much of the analysis and interpretation.
Together, our results clarify the temporal dynamics of neuronal maturation and the evolutionary diversification of their regulatory programs.
October 30, 2025 at 9:42 AM
Together, our results clarify the temporal dynamics of neuronal maturation and the evolutionary diversification of their regulatory programs.
Yet conservation is only part of the story.
Each lineage has elaborated the monoaminergic regulatory program by incorporating additional transcription factors.
Each lineage has elaborated the monoaminergic regulatory program by incorporating additional transcription factors.
October 30, 2025 at 9:42 AM
Yet conservation is only part of the story.
Each lineage has elaborated the monoaminergic regulatory program by incorporating additional transcription factors.
Each lineage has elaborated the monoaminergic regulatory program by incorporating additional transcription factors.
This points to a deeply conserved developmental program for monoaminergic neurons - likely originating more than half a billion years ago in the common ancestor of bilaterians.
October 30, 2025 at 9:42 AM
This points to a deeply conserved developmental program for monoaminergic neurons - likely originating more than half a billion years ago in the common ancestor of bilaterians.
To test whether the conservation of this regulatory program
we re-analysed developmental sc-RNA-seq atlases from zebrafish and sea urchin.
We identified a shared set of transcriptional, probably controlling the early cell-fate commitment, in all three species.
we re-analysed developmental sc-RNA-seq atlases from zebrafish and sea urchin.
We identified a shared set of transcriptional, probably controlling the early cell-fate commitment, in all three species.
October 30, 2025 at 9:42 AM
To test whether the conservation of this regulatory program
we re-analysed developmental sc-RNA-seq atlases from zebrafish and sea urchin.
We identified a shared set of transcriptional, probably controlling the early cell-fate commitment, in all three species.
we re-analysed developmental sc-RNA-seq atlases from zebrafish and sea urchin.
We identified a shared set of transcriptional, probably controlling the early cell-fate commitment, in all three species.
Metabolomic profiling confirmed this temporal sequence: neurotransmitter synthesis begins several hours after transcriptional commitment. However, metabolite levels accumulate at distinct times, suggesting that monoaminergic subtypes mature at different rates.
October 30, 2025 at 9:42 AM
Metabolomic profiling confirmed this temporal sequence: neurotransmitter synthesis begins several hours after transcriptional commitment. However, metabolite levels accumulate at distinct times, suggesting that monoaminergic subtypes mature at different rates.
We found that a small group of transcription factors becomes active in the monoaminergic cell lineage before neurotransmitter enzymes appear, revealing the earliest molecular commitment to monoaminergic identity.
October 30, 2025 at 9:42 AM
We found that a small group of transcription factors becomes active in the monoaminergic cell lineage before neurotransmitter enzymes appear, revealing the earliest molecular commitment to monoaminergic identity.
By combining time-resolved scRNA-seq, trajectory modelling, HCR validation, and metabolomics, we reconstructed the developmental of monoaminergic neurons during Drosophila embryogenesis
October 30, 2025 at 9:42 AM
By combining time-resolved scRNA-seq, trajectory modelling, HCR validation, and metabolomics, we reconstructed the developmental of monoaminergic neurons during Drosophila embryogenesis