Reyes-Lamothe lab
@reyes-lab.bsky.social
We study DNA replication and genome integrity || live-cell single-molecule microscopy || Bacteria || Budding-yeast || mammalian cells
We are at the Department of Biology, @mcgill.ca
https://reyes-lamothe.lab.mcgill.ca
We are at the Department of Biology, @mcgill.ca
https://reyes-lamothe.lab.mcgill.ca
Happy to share our most recent work www.pnas.org/doi/10.1073/.... Here we describe how efficient DNA replication in E. coli is dependent on an interaction between SSB-ssDNA and the DNA polymerase (Pol III). Thanks to all the co-authors for their hard work!
Genome integrity relies on rapid recycling of DNA Pol III in bacteria | PNAS
DNA replication requires precise coordination between DNA unwinding and DNA synthesis.
In all domains of life, protein–protein interactions at the ...
www.pnas.org
November 20, 2025 at 4:54 PM
Happy to share our most recent work www.pnas.org/doi/10.1073/.... Here we describe how efficient DNA replication in E. coli is dependent on an interaction between SSB-ssDNA and the DNA polymerase (Pol III). Thanks to all the co-authors for their hard work!
Reposted by Reyes-Lamothe lab
Check out our new biosensor technology to study DDR kinase signaling: ProKAS.
We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes
ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.
Just published today: rdcu.be/ePNo0
We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes
ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.
Just published today: rdcu.be/ePNo0
November 13, 2025 at 6:22 PM
Check out our new biosensor technology to study DDR kinase signaling: ProKAS.
We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes
ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.
Just published today: rdcu.be/ePNo0
We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes
ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.
Just published today: rdcu.be/ePNo0
Reposted by Reyes-Lamothe lab
Our latest work is out in PNAS!🎉
We show that nonequilibrium polysome dynamics help drive sister chromosomes apart. The closing division septum also contributes via steric interactions.👇
www.pnas.org/doi/10.1073/...
#MicroSky #Microbiology #Biophysics #CellBiology
We show that nonequilibrium polysome dynamics help drive sister chromosomes apart. The closing division septum also contributes via steric interactions.👇
www.pnas.org/doi/10.1073/...
#MicroSky #Microbiology #Biophysics #CellBiology
November 9, 2025 at 2:05 AM
Our latest work is out in PNAS!🎉
We show that nonequilibrium polysome dynamics help drive sister chromosomes apart. The closing division septum also contributes via steric interactions.👇
www.pnas.org/doi/10.1073/...
#MicroSky #Microbiology #Biophysics #CellBiology
We show that nonequilibrium polysome dynamics help drive sister chromosomes apart. The closing division septum also contributes via steric interactions.👇
www.pnas.org/doi/10.1073/...
#MicroSky #Microbiology #Biophysics #CellBiology
Reposted by Reyes-Lamothe lab
New article online: Modulation of archaeal hypernucleosome structure and stability by Mg2+
In this work, we dissect the effects of Mg2+ on hypernucleosomes formed by the canonical histones from M. fervidus (HMfA and HMfB) and T. kodakarensis (HTkA and HTkB).
www.sciencedirect.com/science/arti...
In this work, we dissect the effects of Mg2+ on hypernucleosomes formed by the canonical histones from M. fervidus (HMfA and HMfB) and T. kodakarensis (HTkA and HTkB).
www.sciencedirect.com/science/arti...
Modulation of archaeal hypernucleosome structure and stability by Mg2+
DNA-wrapping histone proteins play a central role in chromatin organization, gene expression and regulation in most eukaryotes and archaea. While the …
www.sciencedirect.com
November 6, 2025 at 7:53 AM
New article online: Modulation of archaeal hypernucleosome structure and stability by Mg2+
In this work, we dissect the effects of Mg2+ on hypernucleosomes formed by the canonical histones from M. fervidus (HMfA and HMfB) and T. kodakarensis (HTkA and HTkB).
www.sciencedirect.com/science/arti...
In this work, we dissect the effects of Mg2+ on hypernucleosomes formed by the canonical histones from M. fervidus (HMfA and HMfB) and T. kodakarensis (HTkA and HTkB).
www.sciencedirect.com/science/arti...
Reposted by Reyes-Lamothe lab
🚨 Publication alert! We are stoked to report the structural basis for activity of a novel class of antimicrobials targeting Gram positive priority pathogens in @natcomms.nature.com. There is something for everyone on this paper, and I will highlight a few things below 👇 1/9 doi.org/10.1038/s414...
A unique inhibitor conformation selectively targets the DNA polymerase PolC of Gram-positive priority pathogens - Nature Communications
In this work, Urem et al. characterize the mode of action as well as mechanism of reduced susceptibility related to a class of antimicrobials that is in development for the treatment of infections wit...
doi.org
November 6, 2025 at 10:29 AM
🚨 Publication alert! We are stoked to report the structural basis for activity of a novel class of antimicrobials targeting Gram positive priority pathogens in @natcomms.nature.com. There is something for everyone on this paper, and I will highlight a few things below 👇 1/9 doi.org/10.1038/s414...
Reposted by Reyes-Lamothe lab
📢 Excited to share that our study has just been published in Nature Communications! We uncover how CRL4-DCAF12 regulates the cellular levels of MCMBP, a chaperone essential for assembling nascent MCM2-7 complexes, to ensure accurate and error-free genome duplication.
October 28, 2025 at 3:04 PM
📢 Excited to share that our study has just been published in Nature Communications! We uncover how CRL4-DCAF12 regulates the cellular levels of MCMBP, a chaperone essential for assembling nascent MCM2-7 complexes, to ensure accurate and error-free genome duplication.
Reposted by Reyes-Lamothe lab
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
October 27, 2025 at 9:43 PM
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
Reposted by Reyes-Lamothe lab
Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....
A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells
Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....
onlinelibrary.wiley.com
October 25, 2025 at 8:56 AM
Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....
Reposted by Reyes-Lamothe lab
Glad to share our paper out today @NatureEcoEvo: “Serial innovations by Asgard archaea shaped the DNA replication machinery of the early eukaryotic ancestor”. www.nature.com/articles/s41... #microsky #archaeasky
Serial innovations by Asgard archaea shaped the DNA replication machinery of the early eukaryotic ancestor - Nature Ecology & Evolution
Phylogenetic and biochemical analyses show a diversity of components of the DNA replication machinery in different Asgard archaea that contributed to the eukaryotic DNA replication machinery.
www.nature.com
October 21, 2025 at 3:06 PM
Glad to share our paper out today @NatureEcoEvo: “Serial innovations by Asgard archaea shaped the DNA replication machinery of the early eukaryotic ancestor”. www.nature.com/articles/s41... #microsky #archaeasky
Reposted by Reyes-Lamothe lab
Hello! The Jacobs-Wagner lab is finally on Bluesky. We’re excited to celebrate science (and scientists!), share our research, and interact with the community. We look forward to connecting with friends and colleagues across the world.
October 19, 2025 at 8:06 PM
Hello! The Jacobs-Wagner lab is finally on Bluesky. We’re excited to celebrate science (and scientists!), share our research, and interact with the community. We look forward to connecting with friends and colleagues across the world.
Reposted by Reyes-Lamothe lab
This was a fun collaboration between Ophélie & the @seegerlab.bsky.social using sybody libraries to target the SMC complex in living bacteria. With a suprising finding: the 14 isolated sybodies bind to the middle of the SMC coiled coil rather than the more conserved ATPase heads.
Single Domain Antibody Inhibitors Target the Coiled Coil Arms of the Bacillus subtilis SMC complex https://www.biorxiv.org/content/10.1101/2025.10.16.682983v1
October 20, 2025 at 8:15 AM
This was a fun collaboration between Ophélie & the @seegerlab.bsky.social using sybody libraries to target the SMC complex in living bacteria. With a suprising finding: the 14 isolated sybodies bind to the middle of the SMC coiled coil rather than the more conserved ATPase heads.
Reposted by Reyes-Lamothe lab
Now in @mdpiopenaccess.bsky.social Bacillus subtilis DinG/XPD-like paralogues, DinG and YpvA, have been implicated in overcoming replication stress. DinG possesses a DEDD exonuclease and DNA helicase domains, whereas YpvA lacks the DEDD exonuclease domain
www.mdpi.com/1422-0067/26...
www.mdpi.com/1422-0067/26...
www.mdpi.com
October 4, 2025 at 10:51 AM
Now in @mdpiopenaccess.bsky.social Bacillus subtilis DinG/XPD-like paralogues, DinG and YpvA, have been implicated in overcoming replication stress. DinG possesses a DEDD exonuclease and DNA helicase domains, whereas YpvA lacks the DEDD exonuclease domain
www.mdpi.com/1422-0067/26...
www.mdpi.com/1422-0067/26...
Reposted by Reyes-Lamothe lab
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle
The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...
www.biorxiv.org
September 25, 2025 at 7:12 AM
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
Reposted by Reyes-Lamothe lab
New PrePrint from the Vecchiarelli and Mosalaganti (@shyamalm.bsky.social) labs! 🧵
Collaboration with the Giessen and Pi labs
Congratulations to co-first authors Y Hoang (@hoanghamyy.bsky.social) and Pankaj Jadhav!
www.biorxiv.org/content/10.1...
Collaboration with the Giessen and Pi labs
Congratulations to co-first authors Y Hoang (@hoanghamyy.bsky.social) and Pankaj Jadhav!
www.biorxiv.org/content/10.1...
Engineering Spatial Control of Bacterial Organelles
Bacteria were once thought to lack organelles, but it is now clear they confine cellular reactions using an array of membrane- and protein-based compartments. A central question, however, is how bacte...
www.biorxiv.org
September 23, 2025 at 5:38 PM
New PrePrint from the Vecchiarelli and Mosalaganti (@shyamalm.bsky.social) labs! 🧵
Collaboration with the Giessen and Pi labs
Congratulations to co-first authors Y Hoang (@hoanghamyy.bsky.social) and Pankaj Jadhav!
www.biorxiv.org/content/10.1...
Collaboration with the Giessen and Pi labs
Congratulations to co-first authors Y Hoang (@hoanghamyy.bsky.social) and Pankaj Jadhav!
www.biorxiv.org/content/10.1...
Reposted by Reyes-Lamothe lab
Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
The eukaryotic replisome intrinsically generates asymmetric daughter chromatin fibers
DNA replication is molecularly asymmetric, due to distinct mechanisms for lagging and leading strand DNA synthesis. Whether chromatin assembly on newly replicated strands is also asymmetric remains un...
www.biorxiv.org
September 20, 2025 at 4:10 PM
Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
Reposted by Reyes-Lamothe lab
Really enjoying reading this primer on single particle tracking #microscopy by @svanteeffelen.bsky.social and colleagues www.nature.com/articles/s43... (pdf on Sven's website)
A guide to single-particle tracking - Nature Reviews Methods Primers
Single-particle tracking is a method for analysing different motion behaviours of individual proteins and protein complexes in live cells. This Primer outlines the experimental setup and labelling tec...
www.nature.com
September 11, 2025 at 8:03 AM
Really enjoying reading this primer on single particle tracking #microscopy by @svanteeffelen.bsky.social and colleagues www.nature.com/articles/s43... (pdf on Sven's website)
Reposted by Reyes-Lamothe lab
New paper out! We show that RecBC remodels single-stranded DNA gaps to protect them from ExoI (sbcB) 3′-5′ exonuclease degradation during replication of damaged DNA.
doi.org/10.1073/pnas...
doi.org/10.1073/pnas...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
doi.org
September 12, 2025 at 7:26 AM
New paper out! We show that RecBC remodels single-stranded DNA gaps to protect them from ExoI (sbcB) 3′-5′ exonuclease degradation during replication of damaged DNA.
doi.org/10.1073/pnas...
doi.org/10.1073/pnas...
Reposted by Reyes-Lamothe lab
Excited to share another study from my lab @fordhamchem now out @narjournal.bsky.social: doi.org/10.1093/nar/.... Most bacterial DNA polymerases must interact with the sliding clamp DnaN for their function, but we show that it’s more complicated in B. subtilis DNA replication. (1/4)
The B. subtilis replicative polymerases bind the sliding clamp with different strengths to tune their activity in DNA replication
Abstract. Ring-shaped sliding clamp proteins are essential components of the replication machinery across all domains of life. DNA polymerases bind the cla
doi.org
August 5, 2025 at 4:57 PM
Excited to share another study from my lab @fordhamchem now out @narjournal.bsky.social: doi.org/10.1093/nar/.... Most bacterial DNA polymerases must interact with the sliding clamp DnaN for their function, but we show that it’s more complicated in B. subtilis DNA replication. (1/4)
Reposted by Reyes-Lamothe lab
Less than two weeks left to apply!
Postdoc positions in my lab to study
1. initiation of DNA replication.
2. chromatin replication/epigenetic inheritance.
Great for biochemists, biophysicists & structural biologists.
Deadline 3 August 2025.
crick.wd3.myworkdayjobs.com/External/job...
Postdoc positions in my lab to study
1. initiation of DNA replication.
2. chromatin replication/epigenetic inheritance.
Great for biochemists, biophysicists & structural biologists.
Deadline 3 August 2025.
crick.wd3.myworkdayjobs.com/External/job...
Postdoctoral Fellow - Costa Lab
Salary for this Role: From £45,500 with benefits, subject to skills and experience. Job Title: Postdoctoral Fellow - Costa Lab Reports to: Alessandro Costa Closing Date: 03/Aug/2025 23.59 GMT Job Desc...
crick.wd3.myworkdayjobs.com
July 21, 2025 at 2:57 PM
Less than two weeks left to apply!
Postdoc positions in my lab to study
1. initiation of DNA replication.
2. chromatin replication/epigenetic inheritance.
Great for biochemists, biophysicists & structural biologists.
Deadline 3 August 2025.
crick.wd3.myworkdayjobs.com/External/job...
Postdoc positions in my lab to study
1. initiation of DNA replication.
2. chromatin replication/epigenetic inheritance.
Great for biochemists, biophysicists & structural biologists.
Deadline 3 August 2025.
crick.wd3.myworkdayjobs.com/External/job...
Reposted by Reyes-Lamothe lab
It is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS
Abstract. Disassembly of the replication machinery (replisome) from chromatin is an active process driven by two ubiquitin ligases Cul2LRR1 and TRAIP, whic
academic.oup.com
July 10, 2025 at 2:55 PM
It is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
Reposted by Reyes-Lamothe lab
Great opportunity for an established scientist to move and grow their research group in beautiful Montreal!
The Canada Excellence Research Chair (CERC) in Climate Change, Environment and Health at @mcgill.ca
Apply by Aug. 8.
Please share widely!
mcgill.wd3.myworkdayjobs.com/en-US/McGill...
The Canada Excellence Research Chair (CERC) in Climate Change, Environment and Health at @mcgill.ca
Apply by Aug. 8.
Please share widely!
mcgill.wd3.myworkdayjobs.com/en-US/McGill...
July 9, 2025 at 3:51 PM
Great opportunity for an established scientist to move and grow their research group in beautiful Montreal!
The Canada Excellence Research Chair (CERC) in Climate Change, Environment and Health at @mcgill.ca
Apply by Aug. 8.
Please share widely!
mcgill.wd3.myworkdayjobs.com/en-US/McGill...
The Canada Excellence Research Chair (CERC) in Climate Change, Environment and Health at @mcgill.ca
Apply by Aug. 8.
Please share widely!
mcgill.wd3.myworkdayjobs.com/en-US/McGill...
Reposted by Reyes-Lamothe lab
Finally published! Many thanks to the reviewers and editor whose comments & suggestions improved this work significantly more than the first preprint version!!!
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
Expanding the diversity of bacterial DNA partitioning: A CTP-independent ParABS system for plasmid partitioning in Streptomyces | PNAS
The ATP- and CTP-dependent ParA-ParB-parS segrosome is a macromolecular complex that
segregates chromosomes/plasmids in most bacterial species. CTP...
www.pnas.org
July 2, 2025 at 5:49 PM
Finally published! Many thanks to the reviewers and editor whose comments & suggestions improved this work significantly more than the first preprint version!!!
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
Reposted by Reyes-Lamothe lab
A must-read for histone afficionados: our new review on 'Histone-mediated chromatin organization in prokaryotes and viruses' www.cell.com/trends/bioch... @samuelschwab.bsky.social @vikramalva.bsky.social
Histone-mediated chromatin organization in prokaryotes and viruses
Histones are fundamental chromatin-organizing proteins in eukaryotes and archaea,
where they assemble into (hyper)nucleosomes that wrap DNA. Recent studies have expanded
the known repertoire of histon...
www.cell.com
June 30, 2025 at 7:35 AM
A must-read for histone afficionados: our new review on 'Histone-mediated chromatin organization in prokaryotes and viruses' www.cell.com/trends/bioch... @samuelschwab.bsky.social @vikramalva.bsky.social
Reposted by Reyes-Lamothe lab
I'm very excited that my first postdoc paper is out now at @nature.com!
www.nature.com/articles/s41...
I developed new single cell CDK sensors to investigate how CDK orchestrates mitosis including investigating bistability/hysteresis, how activity is propagated, and the role of centrosomes.
www.nature.com/articles/s41...
I developed new single cell CDK sensors to investigate how CDK orchestrates mitosis including investigating bistability/hysteresis, how activity is propagated, and the role of centrosomes.
Spatiotemporal orchestration of mitosis by cyclin-dependent kinase - Nature
Experiments in fission yeast show that cyclin-dependent kinase is first activated in the nucleus, from which the mitotic signal is propagated from CDK at the centrosome to the cytoplasm.
www.nature.com
June 25, 2025 at 5:12 PM
I'm very excited that my first postdoc paper is out now at @nature.com!
www.nature.com/articles/s41...
I developed new single cell CDK sensors to investigate how CDK orchestrates mitosis including investigating bistability/hysteresis, how activity is propagated, and the role of centrosomes.
www.nature.com/articles/s41...
I developed new single cell CDK sensors to investigate how CDK orchestrates mitosis including investigating bistability/hysteresis, how activity is propagated, and the role of centrosomes.
Reposted by Reyes-Lamothe lab
CdpA-an archaeal anchor for FtsZ! Excited to be part of this. (FtsZ is easily my favourite protein and the whole larger family in Archaea are intriguing).
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Cell division protein CdpA organises and anchors the midcell ring in haloarchaea - Nature Communications
Cell division in many archaea requires the coordinated activities of two distinct FtsZ proteins, which are part of the midcell division ring. Here, Liao et al. show that an additional protein, CdpA, o...
www.nature.com
June 3, 2025 at 10:44 PM
CdpA-an archaeal anchor for FtsZ! Excited to be part of this. (FtsZ is easily my favourite protein and the whole larger family in Archaea are intriguing).
www.nature.com/articles/s41...
www.nature.com/articles/s41...