Reto Fiolka
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retof.bsky.social
Reto Fiolka
@retof.bsky.social
Optical imaging, interested in STEM topics.
First generation graduate from ETH Zürich
Associate Professor at UT
Southwestern Medical Center
Felix Zhou's u-Segment3D is out:

www.nature.com/articles/s41...

It leverages 2D cell segmentations from orthoviews to generate a ‘consensus’ 3D segmentation.

It does a great job on segmenting densely packed samples, and we have used the algorithm in many applications.
November 12, 2025 at 2:41 AM
3D tracking of mitochondria DNA (green dots) in a HeLa cell.
October 7, 2025 at 3:43 PM
Volumetric imaging of 384 wells in a contact free format using an air lens.

Importantly, the 3D imaging remains diffraction limited and distortion-free, despite the large refractive index mismatch from air to water.
October 6, 2025 at 6:47 PM
Segmented nuclei (gray) and nucleoli (color) in an expanded liver slice:
October 6, 2025 at 6:36 PM
Movies:

Microvili formation and dynamics in T-cells.
October 6, 2025 at 6:35 PM