Reto Fiolka
@retof.bsky.social
Optical imaging, interested in STEM topics.
First generation graduate from ETH Zürich
Associate Professor at UT
Southwestern Medical Center
First generation graduate from ETH Zürich
Associate Professor at UT
Southwestern Medical Center
I may still ask Thorlabs if the controller “knows” the absolute position of the actuator.
It would be annoying if you first had to “zero” the slug each time.
It would be annoying if you first had to “zero” the slug each time.
November 19, 2025 at 11:00 PM
I may still ask Thorlabs if the controller “knows” the absolute position of the actuator.
It would be annoying if you first had to “zero” the slug each time.
It would be annoying if you first had to “zero” the slug each time.
Does it “know” absolute slit position (width)?
The thorlabs stick and slip actuators are normally only relative motion.
The thorlabs stick and slip actuators are normally only relative motion.
November 19, 2025 at 6:34 PM
Does it “know” absolute slit position (width)?
The thorlabs stick and slip actuators are normally only relative motion.
The thorlabs stick and slip actuators are normally only relative motion.
@kevin-dean.bsky.social We can finally nuke those superbugs in the light-sheet chambers!
a man with glasses and a mustache is wearing a white shirt that says finally on it
ALT: a man with glasses and a mustache is wearing a white shirt that says finally on it
media.tenor.com
November 17, 2025 at 4:02 AM
@kevin-dean.bsky.social We can finally nuke those superbugs in the light-sheet chambers!
Nature article: www.nature.com/articles/d41...
Powerful new antibiotic that can kill superbugs discovered in soil bacteria
Surprise discovery could pave the way for new treatments against drug-resistant infections.
www.nature.com
November 16, 2025 at 6:52 PM
Nature article: www.nature.com/articles/d41...
Yes, large fan angles require short focal length lenses.
We made a Plössel lens from two 30mm achromatic doublets.
The short focal length then also results in a tighter lightsheet (for a given aspect ratio).
Light losses are relative. Still better than with massive Gaussian beam expansion.😳
We made a Plössel lens from two 30mm achromatic doublets.
The short focal length then also results in a tighter lightsheet (for a given aspect ratio).
Light losses are relative. Still better than with massive Gaussian beam expansion.😳
November 5, 2025 at 11:35 PM
Yes, large fan angles require short focal length lenses.
We made a Plössel lens from two 30mm achromatic doublets.
The short focal length then also results in a tighter lightsheet (for a given aspect ratio).
Light losses are relative. Still better than with massive Gaussian beam expansion.😳
We made a Plössel lens from two 30mm achromatic doublets.
The short focal length then also results in a tighter lightsheet (for a given aspect ratio).
Light losses are relative. Still better than with massive Gaussian beam expansion.😳
How about we discuss details offline? I have a hard time fitting stuff into character limit here.
November 5, 2025 at 10:51 PM
How about we discuss details offline? I have a hard time fitting stuff into character limit here.
Fan angle matters in the sense that too much angle is hard to pair with existing achromats (and their angular range). We use laser line powell lenses (IIRC 10 and 20 deg).
You indeed can change NA with an adjustable slit. Usually you need to make a relay lens pair to access a Fourier plane.
You indeed can change NA with an adjustable slit. Usually you need to make a relay lens pair to access a Fourier plane.
November 5, 2025 at 8:36 PM
Fan angle matters in the sense that too much angle is hard to pair with existing achromats (and their angular range). We use laser line powell lenses (IIRC 10 and 20 deg).
You indeed can change NA with an adjustable slit. Usually you need to make a relay lens pair to access a Fourier plane.
You indeed can change NA with an adjustable slit. Usually you need to make a relay lens pair to access a Fourier plane.
Let us know if you need any more information.
Our system essentially has a fiberlaser collimator (~1mm beam size), then a powell lens in a rotation mount followed by a short focal length (30-50mm, depending on desired NA) achromatic doublet lens.
After that you already have your sheet.
Our system essentially has a fiberlaser collimator (~1mm beam size), then a powell lens in a rotation mount followed by a short focal length (30-50mm, depending on desired NA) achromatic doublet lens.
After that you already have your sheet.
November 4, 2025 at 5:09 PM
Let us know if you need any more information.
Our system essentially has a fiberlaser collimator (~1mm beam size), then a powell lens in a rotation mount followed by a short focal length (30-50mm, depending on desired NA) achromatic doublet lens.
After that you already have your sheet.
Our system essentially has a fiberlaser collimator (~1mm beam size), then a powell lens in a rotation mount followed by a short focal length (30-50mm, depending on desired NA) achromatic doublet lens.
After that you already have your sheet.
We have built all our current lightsheet systems with a Powell lens.
Much more compact set up and more uniform line intensity distribution compared to cylindrical lenses ( for beam expansion and focusing to a sheet).
Only drawback is that the sheet diverges a bit laterally for different colors.
Much more compact set up and more uniform line intensity distribution compared to cylindrical lenses ( for beam expansion and focusing to a sheet).
Only drawback is that the sheet diverges a bit laterally for different colors.
November 4, 2025 at 1:36 AM
We have built all our current lightsheet systems with a Powell lens.
Much more compact set up and more uniform line intensity distribution compared to cylindrical lenses ( for beam expansion and focusing to a sheet).
Only drawback is that the sheet diverges a bit laterally for different colors.
Much more compact set up and more uniform line intensity distribution compared to cylindrical lenses ( for beam expansion and focusing to a sheet).
Only drawback is that the sheet diverges a bit laterally for different colors.
New Captcha has just dropped: proof that you are not injective.
November 1, 2025 at 6:45 AM
New Captcha has just dropped: proof that you are not injective.
LLMs are injective; one prompt maps to one output, and true also for other way.
So you can get exact prompt back for any LLM generated blurp.
#SparklyLinearAlgebra
So you can get exact prompt back for any LLM generated blurp.
#SparklyLinearAlgebra
November 1, 2025 at 3:37 AM
LLMs are injective; one prompt maps to one output, and true also for other way.
So you can get exact prompt back for any LLM generated blurp.
#SparklyLinearAlgebra
So you can get exact prompt back for any LLM generated blurp.
#SparklyLinearAlgebra
Well done James Hayes, Dylan Burnette, Zach Sanchez and Amy Engevik!
Great microscopy and sample preparation skills!
Great microscopy and sample preparation skills!
October 27, 2025 at 3:07 PM
Well done James Hayes, Dylan Burnette, Zach Sanchez and Amy Engevik!
Great microscopy and sample preparation skills!
Great microscopy and sample preparation skills!