Rachael Ott
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rachaelott.bsky.social
Rachael Ott
@rachaelott.bsky.social
2.5K followers 2.5K following 170 posts
Neurobiologist @UofSC 🔬 Super-resolution #microscopy, adipocytes, cytoskeleton, and #neurodegeneration 🦠
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rachaelott.bsky.social
GFAP/NeuN/DAPI localization seem consistent with published data unfortunately (see below)
Data (from Young, et al., 2019) shows GFAP (green) signal localization in cerebellum of mice Data (from Sachs, et al., 2009—original reference image fed to Gemini 3) shows GFAP (red) and DAPI (blue) signal localization in cerebellum of mice Data (from Huang, et al., 2014) shows NeuN (green, in A) signal localization in cerebellum of mice Data (from Yun, et al., 2025) shows NeuN (cyan) signal localization in rat brain and cerebellum
November 24, 2025 at 11:44 PM
rachaelott.bsky.social
Here’s a side-by-side comparison of the reference image input (from Sachs et al., 2009) and the AI generated figure (below)
November 24, 2025 at 7:49 PM
rachaelott.bsky.social
Gemini 3 created this figure in under 30 seconds from a couple reference images and a one sentence prompt

So I’m at a loss for words, honestly #MicroscopyMonday
This is a 100% AI generated figure created using Gemini 3 (Nano Banana Pro) on Adobe Firefly.
November 24, 2025 at 7:11 PM
rachaelott.bsky.social
For #FluorescenceFriday, α-spectrin (red) and lipid droplets (green) are shown in adipocytes 🔬 #Microscopy #CellBiology
α-spectrin (red), lipid droplets (green), and nuclei (blue) are shown in adipocytes. Scale bar is 20 μm.
October 10, 2025 at 6:37 PM
rachaelott.bsky.social
For #FluorescenceFriday, RhoA (blue) and the actin cytoskeleton (magenta) are shown in a set of primary microglia 🔬 #Neuroscience #Microscopy
RhoA (blue) and the actin cytoskeleton (magenta) are shown in a set of primary microglia.
October 3, 2025 at 1:07 PM
rachaelott.bsky.social
For #FluorescenceFriday, microtubule networks are shown in two adipocytes 🔬 #CellBiology #Microscopy
Microtubules (orange; α-tubulin) and nuclei (blue; DAPI) are shown in two adipocytes, imaged by confocal microscopy.
September 5, 2025 at 6:39 PM
rachaelott.bsky.social
Really great questions, and it might be easier to see here. Lipid droplets occupy a major portion of the cell volume, and likely shape both microtubule dynamics and position of nuclei
June 14, 2025 at 5:30 PM
rachaelott.bsky.social
Yes! Cell fusion occurs during precursor differentiation, producing binucleate and even tetranucleate mature adipocytes
Diagram of adult fat body precursor maturation into adipocytes; adapted from Lei et al., 2023 (https://journals.plos.org/plosbiology/article/figures?id=10.1371/journal.pbio.3002050)
June 13, 2025 at 8:47 PM
rachaelott.bsky.social
For #FluorescenceFriday, this z-projection shows extensive microtubule networks (gold) and nuclei (pink) in adipocytes 🔬 #CellBiology #Microscopy
Microtubules (gold) and nuclei (pink) are shown in a z-projection of adipocytes.
June 13, 2025 at 4:07 PM
rachaelott.bsky.social
#FlyWeek fun for #FluorescenceFriday, nuclei and α-spectrin are shown in this stack of adipocytes from adult Drosophila 🔬 #Microscopy #CellBiology
Nuclei (orange) and the cell membrane (α-spectrin; teal) are shown in adipocytes.
May 9, 2025 at 5:17 PM
rachaelott.bsky.social
For #FluorescenceFriday, actin is shown in a medium spiny neuron 🔬 #Microscopy #Neuroscience
The actin cytoskeleton is shown in a primary medium spiny neuron.
May 2, 2025 at 8:53 PM
rachaelott.bsky.social
For #FluorescenceFriday, a GFP reporter shows nuclei (fire) nestled between lots of lipid droplets in this set of adipocytes 🔬 #Microscopy #CellBiology
Nuclei (fire) and lipid droplets (black; seen by negative staining) are shown in adipocytes.
April 25, 2025 at 3:29 PM
rachaelott.bsky.social
For #FluorescenceFriday, tau (cyan) and neurofilament proteins (fire) are shown in these primary hippocampal neurons 🔬 #Neuroscience #Microscopy
Neurofilament proteins (fire) and tau (cyan) are shown in two primary hippocampal neurons.
April 11, 2025 at 8:31 PM
rachaelott.bsky.social
For #FluorescenceFriday, lipid droplets (orange) and α-spectrin (blue) are shown in adipocytes 🔬 #Microscopy #CellBiology
Lipid droplets (orange; Nile Red), nuclei (cyan; DAPI), and the cell membrane (blue; α-spectrin) are shown in adipocytes.
April 4, 2025 at 3:07 PM
rachaelott.bsky.social
For #FluorescenceFriday, microtubule networks are shown in adipocytes 🔬 #Microscopy #CellBiology
Microtubules (green; alpha-tubulin) and nuclei (blue; DAPI) are shown in adipocytes.
March 28, 2025 at 5:53 PM
rachaelott.bsky.social
For #FluorescenceFriday, tau is shown in a primary hippocampal neuron, using a div LUT from @kwolbachia.bsky.social 🔬 #Microscopy #Neuroscience
Tau protein (green) is shown in a primary hippocampal neuron. The LUT used in this photo is “KTZ_Grey_Div_Green” from the KTZ-LUTs and/or NeuroCytoLUTs update sites on Fiji/ImageJ.
March 14, 2025 at 6:25 PM
rachaelott.bsky.social
For #FluorescenceFriday, microtubules are shown in this massive oligodendrocyte from a primary hippocampal culture 🔬 #Microscopy #Neuroscience
Microtubules (green; α-tubulin) are shown in a primary oligodendrocyte.
February 21, 2025 at 8:29 PM
rachaelott.bsky.social
Happy Valentine’s Day! 💞 For this #FluorescenceFriday, lots of lovely lipid droplets (pink) are shown in adipocytes 🔬 #Microscopy #Science #Valentines
Lipid droplets (pink), nuclei (purple), and the cell membrane (grey; α-spectrin) are shown in adipocytes, edited to create a heart for Valentine’s Day.
February 14, 2025 at 8:17 PM
rachaelott.bsky.social
For #FluorescenceFriday, large lipid droplets (orange) and alpha-spectrin (blue) are shown in adipocytes 🔬 #Microscopy #CellBiology
Lipid droplets (orange), nuclei (cyan; DAPI), and the cell membrane (blue; α-spectrin) are shown in adipocytes.
February 7, 2025 at 4:20 PM
rachaelott.bsky.social
For #FluorescenceFriday, microtubules (magenta) are shown with tau in a primary hippocampal neuron 🔬 #Neuroscience #Microscopy
Microtubules (magenta) and tau protein (cyan/green) are shown in a primary hippocampal neuron.
January 24, 2025 at 8:21 PM
rachaelott.bsky.social
For #FluorescenceFriday, tau (blue) and long loops of neurofilament proteins are shown in a primary hippocampal neuron 🔬 #Neuroscience #Microscopy
Tau (blue) and neurofilament (fire) are shown in a primary hippocampal neuron.
January 17, 2025 at 9:22 PM
rachaelott.bsky.social
Trying out new LUTs from @kwolbachia.bsky.social this #FluorescenceFriday, actin cytoskeleton is shown here in a primary hippocampal neuron 🔬 #Neuroscience #Microscopy
The actin cytoskeleton (blue/green) is shown in a primary hippocampal neuron. The LUT used here is KTZ_bw_SeaWeed.
January 10, 2025 at 7:17 PM
rachaelott.bsky.social
It’s been quite the year on here! Wishing you all a wonderful evening and a happy new year! 💫 #Neuroscience #Microscopy
Tau protein (blue) is shown in a primary hippocampal neuron.
December 31, 2024 at 9:41 PM
rachaelott.bsky.social
For #FluorescenceFriday, tau (cyan) is shown along with microtubules in primary hippocampal neurons 🔬 #neuroscience #microscopy
Tau (cyan) and tubulin (fire) are shown in a primary hippocampal neuron (or two).
December 20, 2024 at 4:40 PM
rachaelott.bsky.social
Tau is one of the most captivating cytoskeletal accessory proteins, shown here in hippocampal neurons for #FluorescenceFriday 🔬
Tau (gold) is shown in primary hippocampal neurons.
December 6, 2024 at 10:15 AM