Pablo J. Sáez
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pjsaez.bsky.social
Pablo J. Sáez
@pjsaez.bsky.social
Husband - Dad of 2 - Scientist 🇨🇱 Chilean expat.
I love to study how cells walk & talk.
PI of the @cellcommlab.bsky.social
#CellMigration #CellComm #calciumsignaling #organelles 🧪🔬
Even if "provisionally" accepted, those are the best words/news I've heard for quite a while...

Here some #blebs to "provisionally" cellebrate.
Let's see this #cell dance under the microscope.

@focalplane.bsky.social #fluorescencefriday #microscopy 🧪🔬
November 21, 2025 at 2:55 PM
I guess it never gets old...bc we love #CellMigration

ATP has many roles: energy currency, hydrotrope, and *danger signal*

Immune cells are faster after ATP exposure, F-actin (cyan) goes to the rear for nitro boost!

www.science.org/doi/full/10....
@focalplane.bsky.social #fluorescencefriday 🧪🔬
November 14, 2025 at 8:38 PM
I love to see the endoplasmic reticulum (ER, cyan) hitchhiking over microtubules (MTs, orange).

Also the membrane dynamics are lovely.

It's been a while, but here we are for a late #fluorescencefriday with some #microscopy
#scicomm #scisky @cellcommlab.bsky.social focalplane.bsky.social 🧪🔬
November 7, 2025 at 9:25 PM
Kudos to @eliasbarrigalab.bsky.social for the Innovation DGZ Prize for his work on #CellMigration

A great Chilean 🇨🇱 in #STEM 💪🏽🔥

@dgz.bsky.social #DGZ50
October 27, 2025 at 5:26 PM
Today 20 years after I started working in a scientific lab while still in 🇨🇱, my lab, the @cellcommlab.bsky.social has its 5th labversary.

What a ride ! I'm thankful for the support of my family, friends, & so many great collabs & colleagues.

Fig legend 👇🏽 + pending longer thread #PIlife #Science
September 1, 2025 at 10:00 PM
Post an image from your summer that confers peace
August 31, 2025 at 6:11 PM
Barely made ir for another #FluorescenceFriday

Ahhh the #Nucleus ... it never gets old.
This #cell has 2 bc why not !! It seems they are refusing to separate.

This 👇🏽 is an excellent chance to put your last work in the nucleus in this issue edited by @abbybuch.bsky.social @jcellsci.bsky.social
July 25, 2025 at 10:01 PM
#HotFromThePress !!

What if instead of analyzing 4-6 pathology markers you could check >100 in the *same* sample?

@maltekuehl.com & co @puellesv.bsky.social lab introduce #PathoPlex, method that could even predict disease

Full www.nature.com/articles/s41...
@focalplane.bsky.social @nature.com 🧪🔬
July 18, 2025 at 1:14 PM
This is why we love #cellmigration

#Leukocytes: #Neutrophils (cyan) #Macrophages (orange) chemotaxis.

I couldn't share it before the retirement of @zebrafishrock.bsky.social, but cool #microscopy in #zebrafish continue to rock !

#scicomm #scisky @cellcommlab.bsky.social @focalplane.bsky.social 🧪🔬
July 8, 2025 at 10:18 AM
Post yourself from another era.

(20 years ago)
June 29, 2025 at 6:38 PM
Do you know what happens if you squeeze a #cell?

If the #confinement is high, the nucleus (green) will be super tense & the compressed cells will react like this👇🏽changing their #cytoskeleton (magenta)

Full: www.science.org/doi/10.1126/...
#scicomm #microscopy #sciencesky @focalplane.bsky.social 🧪🔬
June 28, 2025 at 12:32 PM
#FluorescenceFriday

#Mitochondria, powerhouse of the #cell were labeled (cyan) and it's possible to visualize how they reach *every* corner to provide energy.

All recorded under the microscope @cellcommlab.bsky.social

#scicomm #sciencesky #microscopy #organelles @focalplane.bsky.social 🧪🔬
June 20, 2025 at 7:24 PM
This is why we love #cell dynamics !

Look at this #macrophage branching exploring the surroundings videoed under the microscope

This what immune cells do in our body in search of bacteria, viruses and death cells

#microscopy #cellbio #sciencesky @focalplane.bsky.social @cellcommlab.bsky.social 🧪🔬
June 7, 2025 at 10:14 PM
It was a great @dgz.bsky.social session with a lot of #CellMigration and trafficking of #ImmuneCells

Thanks to all the speakers: @liz-kurtz.bsky.social, #ZahraaA, @labliston.bsky.social and #StefanU
April 29, 2025 at 12:26 PM
100 years ago it was founded the most successful and popular football team in Chile 🇨🇱: #ColoColo ⚽🏆

This year I had the chance to be in, Valladolid, where David Arellano (the the founder of the club) died (Hotel Inglaterra).
He died while playing for Colo-Colo.

#centenarioalbo #ColoColo100
April 19, 2025 at 3:44 PM
Hot from the press: #CellMigration #vesicles #organelles

Jeppesen et al @mag2art.bsky.social lab introduce blebbisomes: organelle-rich motile vesicles, have immune checkpoint inhibitors, released by #cancer cells & used for #cellcomm

@naturecellbiology.bsky.social www.nature.com/articles/s41... 🧪🔬
February 22, 2025 at 6:53 AM
An oldie (10 y!) but goodie: cellular interaction at the top! #cellcomm

Video of a T cell (orange) interacting with a target cell (blue)

From Chen et al. @ Betzig lab (@hhmijanelia.bsky.social) game changing paper !!

Full science.org/doi/10.1126/... @focalplane.bsky.social #scicomm #microscopy 🧪🔬
February 18, 2025 at 8:38 PM
Hot from the press for #CellMigration + #Nucleus aficionados

Karling @helenweavers.bsky.social lab show *in vivo* how immune cell adapt quickly to confined microenvironments during motility.

Live-cell imaging, image analysis, #Drosophila !

Full @emboreports.org
www.embopress.org/doi/full/10....
February 10, 2025 at 5:50 AM
#CellMigration happens all the time in our bodies. This may (or not) rely on adhesion molecules

#Cancer cells switch strategies during metastasis to invade other tissues

Naomi Wee (naomiwee.com) did this neat movie to explain it:
www.youtube.com/watch?v=D9aD...
#SciSky 🧪🔬 @cellcommlab.bsky.social
February 6, 2025 at 10:44 AM
The #nucleus contains the info of a #cell, but it does much more than that !!

Since the nucleus is *big*, acts as the main intracellular obstacle, and during #CellMigration can get 'trapped'/stuck until the cell pushes it FWD.

#scicomm @focalplane.bsky.social @cellcommlab.bsky.social
#Skyscience
February 1, 2025 at 3:45 PM
How cells decide where to go? #CellMigration

Last year we published our 1st paper @cellcommlab.bsky.social, showing how actin-based membrane oscillations define a new direction ( #cancer & non-cancer #cells )

Full www.nature.com/articles/s41...
@naturephysics.bsky.social @focalplane.bsky.social 🧪🔬
January 18, 2025 at 11:24 PM
#CellMigration meets #SciArt

Naomi Wee (naomiwee.com) did this great movie to explain how amoeboid migration works (also mesenchymal & cancer migration)

Wanna know what happens if this leukocyte chooses the wrong path? Watch this !

www.youtube.com/watch?v=mPwn... @focalplane.bsky.social #SciSky
January 13, 2025 at 9:01 AM
#CellMigration aficionados, it would be cool to have a similar video for amoeboid cell migration too, no?

Well come tomorrow and see the nice work done by Naomi Wee.

Early teaser below, you can already see membrane dynamics and the leading edge vs uropod shape

#GRCCellMigration #SciArt #GRC
January 12, 2025 at 4:34 PM
How from the #preprint, super cool story on the role of #cytoskeleton by my former lab

Spearheaded by dear friend and cell biologist extraordinaire Mathieu Deygas (Piel, Vargas lab @institutcurie.bsky.social)

Data from in vitro to in vivo.

Full at www.biorxiv.org/content/10.1...
#scicomm #SciSky
January 10, 2025 at 4:24 PM
Warming up for the upcoming #GRCCellMigration

For me the best video to explain adhesion-dependent #CellMigration by Felix Donghwi Son

More in the website felixvis.artstation.com #sciart #scicomm #ScienceSky #SciSky #cytoskeleton #FocalAdhesion
January 9, 2025 at 11:53 PM