Piotr Kolata
@piotrkolata.bsky.social
Postdoc at @mrclmb.bsky.social @matteoall.bsky.social group. Studying #nuclearenvelope across scales #teamtomo and team #cryoEM ❄️🔬
previously @viblifesciences.bsky.social, Rouslan Efremov group, respiratory complex I
previously @viblifesciences.bsky.social, Rouslan Efremov group, respiratory complex I
Reposted by Piotr Kolata
"Runctitiononal features"? "Medical fymblal"? "1 Tol Line storee"? This gets worse the longer you look at it. But it's got to be good, because it was published in Nature Scientific Reports last week: www.nature.com/articles/s41... h/t @asa.tsbalans.se
November 27, 2025 at 9:30 AM
"Runctitiononal features"? "Medical fymblal"? "1 Tol Line storee"? This gets worse the longer you look at it. But it's got to be good, because it was published in Nature Scientific Reports last week: www.nature.com/articles/s41... h/t @asa.tsbalans.se
Reposted by Piotr Kolata
How does fever work?
Our new Science paper shows how elevated body temperature can protect against severe influenza and that avian-origin viruses escape this defence.
This is likely one reason why bird flus and some pandemic influenzas can be so severe.🧵
www.science.org/doi/10.1126/...
Our new Science paper shows how elevated body temperature can protect against severe influenza and that avian-origin viruses escape this defence.
This is likely one reason why bird flus and some pandemic influenzas can be so severe.🧵
www.science.org/doi/10.1126/...
Avian-origin influenza A viruses tolerate elevated pyrexic temperatures in mammals
Host body temperature can define a virus’s replicative profile—influenza A viruses (IAVs) adapted to 40° to 42°C in birds are less temperature sensitive in vitro compared with human isolates adapted t...
www.science.org
November 27, 2025 at 7:55 PM
How does fever work?
Our new Science paper shows how elevated body temperature can protect against severe influenza and that avian-origin viruses escape this defence.
This is likely one reason why bird flus and some pandemic influenzas can be so severe.🧵
www.science.org/doi/10.1126/...
Our new Science paper shows how elevated body temperature can protect against severe influenza and that avian-origin viruses escape this defence.
This is likely one reason why bird flus and some pandemic influenzas can be so severe.🧵
www.science.org/doi/10.1126/...
Reposted by Piotr Kolata
Microsecond Time-Resolved Cryo-EM Based on Jet Vitrification https://www.biorxiv.org/content/10.1101/2025.11.21.689681v1
November 22, 2025 at 2:47 AM
Microsecond Time-Resolved Cryo-EM Based on Jet Vitrification https://www.biorxiv.org/content/10.1101/2025.11.21.689681v1
Reposted by Piotr Kolata
Oh wow, this is 🤯 amazing: ~3-fold acceleration of running #relion on A100 GPUs for realistic single-particle refinement by the group of Guangwen Yang at @tsinghuauniversity.bsky.social
.
What a fantastic example of #OpenSoftwareAcceleratesScience, literally! 😍
dl.acm.org/doi/full/10....
.
What a fantastic example of #OpenSoftwareAcceleratesScience, literally! 😍
dl.acm.org/doi/full/10....
T2-RELION: Task Parallelism, Tensor Core Accelerated RELION for Cryo-EM 3D Reconstruction | Proceedings of the International Conference for High Performance Computing, Networking, Storage and Analysis
dl.acm.org
November 21, 2025 at 12:32 PM
Oh wow, this is 🤯 amazing: ~3-fold acceleration of running #relion on A100 GPUs for realistic single-particle refinement by the group of Guangwen Yang at @tsinghuauniversity.bsky.social
.
What a fantastic example of #OpenSoftwareAcceleratesScience, literally! 😍
dl.acm.org/doi/full/10....
.
What a fantastic example of #OpenSoftwareAcceleratesScience, literally! 😍
dl.acm.org/doi/full/10....
Reposted by Piotr Kolata
Tired of knowing what you are looking for in your tomograms?
Why not try bluesky-less Frosina's new self-supervised algorithm for semantic segmentation and particle picking for #teamtomo (includes a new denoiser without the need for odd/even tomos)
Code is on GitHub if you want to try it out
Why not try bluesky-less Frosina's new self-supervised algorithm for semantic segmentation and particle picking for #teamtomo (includes a new denoiser without the need for odd/even tomos)
Code is on GitHub if you want to try it out
November 19, 2025 at 2:04 PM
Tired of knowing what you are looking for in your tomograms?
Why not try bluesky-less Frosina's new self-supervised algorithm for semantic segmentation and particle picking for #teamtomo (includes a new denoiser without the need for odd/even tomos)
Code is on GitHub if you want to try it out
Why not try bluesky-less Frosina's new self-supervised algorithm for semantic segmentation and particle picking for #teamtomo (includes a new denoiser without the need for odd/even tomos)
Code is on GitHub if you want to try it out
Reposted by Piotr Kolata
Nature research paper: Rare microbial relict sheds light on an ancient eukaryotic supergroup
go.nature.com/486YtjW
go.nature.com/486YtjW
Rare microbial relict sheds light on an ancient eukaryotic supergroup - Nature
The discovery of an unusual protist named Solarion arienae, which has a mitochondrial genome with some intriguing features, provides insight into the early radiation of eukaryotic groups.
go.nature.com
November 20, 2025 at 11:04 AM
Nature research paper: Rare microbial relict sheds light on an ancient eukaryotic supergroup
go.nature.com/486YtjW
go.nature.com/486YtjW
Reposted by Piotr Kolata
Science has become obsessed with publishing numbers. With this new satirical proposal, have we reached peak metric?
go.nature.com/3JUWNSs
go.nature.com/3JUWNSs
Introducing the j-metric: a true measure of what matters in academia
Science has become obsessed with publishing numbers. With this new satirical proposal, have we reached peak metric?
go.nature.com
November 18, 2025 at 2:37 PM
Science has become obsessed with publishing numbers. With this new satirical proposal, have we reached peak metric?
go.nature.com/3JUWNSs
go.nature.com/3JUWNSs
Reposted by Piotr Kolata
Peanut allergies are plummeting in children, and you can thank science. Early exposure has turned out to be one of the best prevention methods, by @picardonhealth.bsky.social www.theglobeandmail.com/opinion/arti... via @theglobeandmail.com
Opinion: Peanut allergies are plummeting in children, and you can thank science
Early exposure has turned out to be one of the best prevention methods for allergies in kids
www.theglobeandmail.com
November 18, 2025 at 12:57 PM
Peanut allergies are plummeting in children, and you can thank science. Early exposure has turned out to be one of the best prevention methods, by @picardonhealth.bsky.social www.theglobeandmail.com/opinion/arti... via @theglobeandmail.com
Reposted by Piotr Kolata
How does the well tolerated anti-hyperglycemic drug #metformin inhibit mitochondrial complex I?
In our new paper, we use structures, MD and kinetics to show that metformin inhibits by a distinct mechanism that more hydrophobic, less well tolerated, biguanides.
www.nature.com/articles/s41...
In our new paper, we use structures, MD and kinetics to show that metformin inhibits by a distinct mechanism that more hydrophobic, less well tolerated, biguanides.
www.nature.com/articles/s41...
Hydrophilic metformin and hydrophobic biguanides inhibit mitochondrial complex I by distinct mechanisms - Nature Structural & Molecular Biology
He, Teng and Yang et al. report how metformin, the widely used antidiabetic drug, inhibit its target, the respiratory complex I, through a distinct state-dependent, inhibitor trapping mechanism, thus ...
www.nature.com
November 10, 2025 at 6:35 PM
How does the well tolerated anti-hyperglycemic drug #metformin inhibit mitochondrial complex I?
In our new paper, we use structures, MD and kinetics to show that metformin inhibits by a distinct mechanism that more hydrophobic, less well tolerated, biguanides.
www.nature.com/articles/s41...
In our new paper, we use structures, MD and kinetics to show that metformin inhibits by a distinct mechanism that more hydrophobic, less well tolerated, biguanides.
www.nature.com/articles/s41...
Reposted by Piotr Kolata
How is the integrated stress response terminated?
@annebertolotti.bsky.social’s group have revealed the mechanism of termination of the ISR, answering a decades-old question surrounding the ISR pathway, which is crucial to cellular homeostasis.
www2.mrc-lmb.cam.ac.uk/how-is-the-i...
#LMBReserch
@annebertolotti.bsky.social’s group have revealed the mechanism of termination of the ISR, answering a decades-old question surrounding the ISR pathway, which is crucial to cellular homeostasis.
www2.mrc-lmb.cam.ac.uk/how-is-the-i...
#LMBReserch
November 14, 2025 at 9:30 AM
How is the integrated stress response terminated?
@annebertolotti.bsky.social’s group have revealed the mechanism of termination of the ISR, answering a decades-old question surrounding the ISR pathway, which is crucial to cellular homeostasis.
www2.mrc-lmb.cam.ac.uk/how-is-the-i...
#LMBReserch
@annebertolotti.bsky.social’s group have revealed the mechanism of termination of the ISR, answering a decades-old question surrounding the ISR pathway, which is crucial to cellular homeostasis.
www2.mrc-lmb.cam.ac.uk/how-is-the-i...
#LMBReserch
Reposted by Piotr Kolata
MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony.
Or from a single dish of HEK cell culture in the case of two membrane proteins.
Out in Nature Methods now! lnkd.in/gpyBSceg
Wonderful collaboration with the Efremov lab.
Or from a single dish of HEK cell culture in the case of two membrane proteins.
Out in Nature Methods now! lnkd.in/gpyBSceg
Wonderful collaboration with the Efremov lab.
November 14, 2025 at 6:38 PM
MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony.
Or from a single dish of HEK cell culture in the case of two membrane proteins.
Out in Nature Methods now! lnkd.in/gpyBSceg
Wonderful collaboration with the Efremov lab.
Or from a single dish of HEK cell culture in the case of two membrane proteins.
Out in Nature Methods now! lnkd.in/gpyBSceg
Wonderful collaboration with the Efremov lab.
Reposted by Piotr Kolata
New paper alert! Scientists in Clemens Plaschka’s lab at the IMP and @juliusbrennecke.bsky.social's lab at
@imbavienna.bsky.social solved a decade-old puzzle, uncovering how the information molecule mRNA travels from the cell’s nucleus to its periphery. More: bit.ly/4nHcvys
@imbavienna.bsky.social solved a decade-old puzzle, uncovering how the information molecule mRNA travels from the cell’s nucleus to its periphery. More: bit.ly/4nHcvys
November 6, 2025 at 4:04 PM
New paper alert! Scientists in Clemens Plaschka’s lab at the IMP and @juliusbrennecke.bsky.social's lab at
@imbavienna.bsky.social solved a decade-old puzzle, uncovering how the information molecule mRNA travels from the cell’s nucleus to its periphery. More: bit.ly/4nHcvys
@imbavienna.bsky.social solved a decade-old puzzle, uncovering how the information molecule mRNA travels from the cell’s nucleus to its periphery. More: bit.ly/4nHcvys
Reposted by Piotr Kolata
Helicon: Helical parameter determination and 3D reconstruction from one image pubmed.ncbi.nlm.nih.gov/41130582/ #cryoem
October 24, 2025 at 2:50 PM
Helicon: Helical parameter determination and 3D reconstruction from one image pubmed.ncbi.nlm.nih.gov/41130582/ #cryoem
Reposted by Piotr Kolata
Excited to share a new chapter of my scientific journey in Chris Russo's group — developing a way to freeze proteins faster than they diffuse, paving the way for cryo-EM without air–water interface damage.
Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
October 22, 2025 at 9:33 PM
Excited to share a new chapter of my scientific journey in Chris Russo's group — developing a way to freeze proteins faster than they diffuse, paving the way for cryo-EM without air–water interface damage.
Reposted by Piotr Kolata
Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
October 22, 2025 at 4:41 PM
Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Reposted by Piotr Kolata
Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Icecream: High-Fidelity Equivariant Cryo-Electron Tomography
Cryo-electron tomography (cryo-ET) visualizes 3D cellular architecture in near-native states. Recent deep-learning methods (CryoCARE, IsoNet, DeepDeWedge, CryoLithe) improve denoising and artifact cor...
www.biorxiv.org
October 23, 2025 at 11:13 AM
Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Reposted by Piotr Kolata
I just made an account and voted for this biology laboratory made of LEGO to "promote biological research and inspire more people into the world of biology". They need 10K votes. Reposting and/or voting below would be helpful. 🤗
beta.ideas.lego.com/product-idea...
beta.ideas.lego.com/product-idea...
Biological Laboratory | LEGO® Ideas
This is a two-floor realistic Biological Laboratory building. In this design, I try to tell stories about biologists, introduce biology concepts to general publ…
beta.ideas.lego.com
October 16, 2025 at 8:07 AM
I just made an account and voted for this biology laboratory made of LEGO to "promote biological research and inspire more people into the world of biology". They need 10K votes. Reposting and/or voting below would be helpful. 🤗
beta.ideas.lego.com/product-idea...
beta.ideas.lego.com/product-idea...
Reposted by Piotr Kolata
Huge congratulations to Wieczorek Lab PhD student Bin Cai and now-PI Jingwei Xu @xujwet.bsky.social on figuring out the surprisingly complex structure of the A-C linker crosslinking microtubule triplets in Tetrahymena basal body centrioles! www.science.org/doi/10.1126/...
Structure and assembly of the A-C linker connecting microtubule triplets in centrioles
Multiscale cryo-EM of centrioles reveals the structure and polymorphism of the A-C linker connecting microtubule triplets.
www.science.org
October 10, 2025 at 1:14 PM
Huge congratulations to Wieczorek Lab PhD student Bin Cai and now-PI Jingwei Xu @xujwet.bsky.social on figuring out the surprisingly complex structure of the A-C linker crosslinking microtubule triplets in Tetrahymena basal body centrioles! www.science.org/doi/10.1126/...
Reposted by Piotr Kolata
Correlative MS Imaging for cellular identification and analysis of in situ cryo-ET www.biorxiv.org/content/10.1101/2025.09.16.676641v1 #cryoem
September 19, 2025 at 9:16 AM
Correlative MS Imaging for cellular identification and analysis of in situ cryo-ET www.biorxiv.org/content/10.1101/2025.09.16.676641v1 #cryoem
Reposted by Piotr Kolata
New Online! The advent of confocal laser scanning microscopy in biological research
The advent of confocal laser scanning microscopy in biological research
Nature Reviews Molecular Cell Biology, Published online: 01 October 2025; doi:10.1038/s41580-025-00905-5The advent of laser-scanning confocal microscopy revolutionized cell biology, offering unprecedented resolution and depth and enabling scientists to visualize cellular structures in 3D.
bit.ly
October 1, 2025 at 12:03 PM
New Online! The advent of confocal laser scanning microscopy in biological research
Reposted by Piotr Kolata
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoem
September 17, 2025 at 8:49 AM
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoem
Reposted by Piotr Kolata
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 13, 2025 at 12:05 AM
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
Reposted by Piotr Kolata
PickET: An unsupervised method for localizing macromolecules in cryo-electron tomograms pubmed.ncbi.nlm.nih.gov/40894555/ #cryoem
September 4, 2025 at 5:08 AM
PickET: An unsupervised method for localizing macromolecules in cryo-electron tomograms pubmed.ncbi.nlm.nih.gov/40894555/ #cryoem
Reposted by Piotr Kolata
Now published @cp-cellreports.bsky.social! Antibody-mediated TGF-β1 activation for the treatment of diseases caused by deleterious T cell activity: www.cell.com/cell-reports...
Fantastic collaboration & massive effort from Sophie Lucas Lab @ de Duve Institute. #CryoEM with @savvideslab.bsky.social.
Fantastic collaboration & massive effort from Sophie Lucas Lab @ de Duve Institute. #CryoEM with @savvideslab.bsky.social.
September 2, 2025 at 11:25 AM
Now published @cp-cellreports.bsky.social! Antibody-mediated TGF-β1 activation for the treatment of diseases caused by deleterious T cell activity: www.cell.com/cell-reports...
Fantastic collaboration & massive effort from Sophie Lucas Lab @ de Duve Institute. #CryoEM with @savvideslab.bsky.social.
Fantastic collaboration & massive effort from Sophie Lucas Lab @ de Duve Institute. #CryoEM with @savvideslab.bsky.social.
Reposted by Piotr Kolata
Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️
August 30, 2025 at 2:11 PM
Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️