Pablo Carravilla
@pablo-carravilla.bsky.social
Group leader at Biofisika Institute biofisika.org
Cell membranes, biophysics, microscopy, rare diseases.
Cell membranes, biophysics, microscopy, rare diseases.
Reposted by Pablo Carravilla
Out today, we report how small chemical modifications in the metabolism of ergosterol are needed for yeast membranes to phase seperate into ordered yet fluid domains www.science.org/doi/10.1126/... (1/n)
Structural dissection of ergosterol metabolism reveals a pathway optimized for membrane phase separation
The metabolism of ergosterol shapes this lipid to allow the formation of fluid membrane domains in yeast cells.
www.science.org
April 24, 2025 at 3:04 PM
Out today, we report how small chemical modifications in the metabolism of ergosterol are needed for yeast membranes to phase seperate into ordered yet fluid domains www.science.org/doi/10.1126/... (1/n)
Reposted by Pablo Carravilla
Hey gang, stop what you’re doing, new organelle just dropped!!
Amazing the kind of stuff one finds inside cells by just looking at them (with magic like cryoET). What the heck are all these strange hemifused vesicles?
Amazing the kind of stuff one finds inside cells by just looking at them (with magic like cryoET). What the heck are all these strange hemifused vesicles?
Early for #MicroscopyMonday: #Hemifusomes- Newly discovered ESCRT-independent intermediates of intraluminal vesicle and multivesicular body formation! In collaboration with the Kachar Lab at the NIH and out today in @natcomms.nature.com : rdcu.be/emtQu
#cryoET #trafficking #organelle #hemifusion
#cryoET #trafficking #organelle #hemifusion
May 19, 2025 at 4:06 PM
Hey gang, stop what you’re doing, new organelle just dropped!!
Amazing the kind of stuff one finds inside cells by just looking at them (with magic like cryoET). What the heck are all these strange hemifused vesicles?
Amazing the kind of stuff one finds inside cells by just looking at them (with magic like cryoET). What the heck are all these strange hemifused vesicles?
Reposted by Pablo Carravilla
🚨 Postdoc Opportunity #2 🚨
We are looking for candidates with a strong background in molecular dynamics simulations of membrane protein interactions to unravel the role of lipids in CD95 oligomerization and signaling!
Please apply by March 28!
#LipidTime @bzh-hd.bsky.social
We are looking for candidates with a strong background in molecular dynamics simulations of membrane protein interactions to unravel the role of lipids in CD95 oligomerization and signaling!
Please apply by March 28!
#LipidTime @bzh-hd.bsky.social
#PostDocCalls Project #85:
˚⋆🔬Lipid-Mediated Regulation of CD95 #Oligomerization and Signaling🧪🥽🧠⋆˚
Applications on the Alliance website:
www.health-life-sciences.de/opportunitie...
@dkfz.bsky.social
@fabiololicato.com @bzh-hd.bsky.social
@hlsalliance.bsky.social
#LipidTime #ProteinDesign
˚⋆🔬Lipid-Mediated Regulation of CD95 #Oligomerization and Signaling🧪🥽🧠⋆˚
Applications on the Alliance website:
www.health-life-sciences.de/opportunitie...
@dkfz.bsky.social
@fabiololicato.com @bzh-hd.bsky.social
@hlsalliance.bsky.social
#LipidTime #ProteinDesign
March 6, 2025 at 3:43 PM
🚨 Postdoc Opportunity #2 🚨
We are looking for candidates with a strong background in molecular dynamics simulations of membrane protein interactions to unravel the role of lipids in CD95 oligomerization and signaling!
Please apply by March 28!
#LipidTime @bzh-hd.bsky.social
We are looking for candidates with a strong background in molecular dynamics simulations of membrane protein interactions to unravel the role of lipids in CD95 oligomerization and signaling!
Please apply by March 28!
#LipidTime @bzh-hd.bsky.social
Reposted by Pablo Carravilla
📢 SAVE THE DATE: Sept 29 - Oct 1, 2025
and come to Berlin to the
BIOMEMBRANE DAYS
Excellent line of speakers and slots for contributed talks.
Registration will open in Spring.
biomembrane-days-2025.mpikg.mpg.de
Organized by [email protected] Thomas Weikl and myself
@mpici.bsky.social
and come to Berlin to the
BIOMEMBRANE DAYS
Excellent line of speakers and slots for contributed talks.
Registration will open in Spring.
biomembrane-days-2025.mpikg.mpg.de
Organized by [email protected] Thomas Weikl and myself
@mpici.bsky.social
February 26, 2025 at 12:52 PM
📢 SAVE THE DATE: Sept 29 - Oct 1, 2025
and come to Berlin to the
BIOMEMBRANE DAYS
Excellent line of speakers and slots for contributed talks.
Registration will open in Spring.
biomembrane-days-2025.mpikg.mpg.de
Organized by [email protected] Thomas Weikl and myself
@mpici.bsky.social
and come to Berlin to the
BIOMEMBRANE DAYS
Excellent line of speakers and slots for contributed talks.
Registration will open in Spring.
biomembrane-days-2025.mpikg.mpg.de
Organized by [email protected] Thomas Weikl and myself
@mpici.bsky.social
Reposted by Pablo Carravilla
New paper out 🎇
It has been 13 years since @owenlabuob.bsky.social and co. published a stellar protocol on how to measure membrane order with smart probes! Since then, there has been new dyes, imaging modes and biology! We wrote a protocol for Nature Protocols:
www.nature.com/articles/s41...
It has been 13 years since @owenlabuob.bsky.social and co. published a stellar protocol on how to measure membrane order with smart probes! Since then, there has been new dyes, imaging modes and biology! We wrote a protocol for Nature Protocols:
www.nature.com/articles/s41...
Measuring plasma membrane fluidity using confocal microscopy - Nature Protocols
Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...
www.nature.com
February 19, 2025 at 3:58 PM
New paper out 🎇
It has been 13 years since @owenlabuob.bsky.social and co. published a stellar protocol on how to measure membrane order with smart probes! Since then, there has been new dyes, imaging modes and biology! We wrote a protocol for Nature Protocols:
www.nature.com/articles/s41...
It has been 13 years since @owenlabuob.bsky.social and co. published a stellar protocol on how to measure membrane order with smart probes! Since then, there has been new dyes, imaging modes and biology! We wrote a protocol for Nature Protocols:
www.nature.com/articles/s41...
We wrote a protocol to quantify plasma membrane fluidity using a confocal microscope 🔬
Hopefully those interested in measuring membrane biophysical properties in live cells find it useful.
We also provide an ImageJ/Fiji tool to make the analysis as easy as possible.
www.nature.com/articles/s41...
Hopefully those interested in measuring membrane biophysical properties in live cells find it useful.
We also provide an ImageJ/Fiji tool to make the analysis as easy as possible.
www.nature.com/articles/s41...
Measuring plasma membrane fluidity using confocal microscopy - Nature Protocols
Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...
www.nature.com
February 19, 2025 at 4:54 PM
We wrote a protocol to quantify plasma membrane fluidity using a confocal microscope 🔬
Hopefully those interested in measuring membrane biophysical properties in live cells find it useful.
We also provide an ImageJ/Fiji tool to make the analysis as easy as possible.
www.nature.com/articles/s41...
Hopefully those interested in measuring membrane biophysical properties in live cells find it useful.
We also provide an ImageJ/Fiji tool to make the analysis as easy as possible.
www.nature.com/articles/s41...
Reposted by Pablo Carravilla
The most frequently asked question we get: do #lipidtime genetically encoded lipid biosensors compete out cells’ native effector proteins?
Victoria Holmes and Morgan Ricci led a paper in JCB that answers this question:
rupress.org/jcb/article/...
Read on for a brief thread.
Victoria Holmes and Morgan Ricci led a paper in JCB that answers this question:
rupress.org/jcb/article/...
Read on for a brief thread.
Single-molecule lipid biosensors mitigate inhibition of endogenous effector proteins | Journal of Cell Biology | Rockefeller University Press
Holmes and colleagues show that AKT activation is blocked by commonly used PIP3 biosensors, but that this can be overcome by expressing biosensors at singl
rupress.org
February 11, 2025 at 4:03 PM
The most frequently asked question we get: do #lipidtime genetically encoded lipid biosensors compete out cells’ native effector proteins?
Victoria Holmes and Morgan Ricci led a paper in JCB that answers this question:
rupress.org/jcb/article/...
Read on for a brief thread.
Victoria Holmes and Morgan Ricci led a paper in JCB that answers this question:
rupress.org/jcb/article/...
Read on for a brief thread.
Thanks for your support throughout my research journey Erdinc, you have been a true inspiration for me!
New PI born 🎁 In case you are one of the few who doesn't know @pablo-carravilla.bsky.social , he is starting his lab in Bilbao! Having Pablo in the lab as postdoc was a true privilege! Vividly remembering the first time we discussed this (Fig 1a) and today when he is ready to be a leader (Fig 1b).
January 26, 2025 at 6:46 PM
Thanks for your support throughout my research journey Erdinc, you have been a true inspiration for me!
Reposted by Pablo Carravilla
How does nano-scale plasma membrane structure underpin tissue development?
Let’s find out!
First opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP and University of Warwick.
🔬✨🐟📸
More info here:
www.findaphd.com/phds/project...
Let’s find out!
First opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP and University of Warwick.
🔬✨🐟📸
More info here:
www.findaphd.com/phds/project...
December 16, 2024 at 2:29 PM
How does nano-scale plasma membrane structure underpin tissue development?
Let’s find out!
First opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP and University of Warwick.
🔬✨🐟📸
More info here:
www.findaphd.com/phds/project...
Let’s find out!
First opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP and University of Warwick.
🔬✨🐟📸
More info here:
www.findaphd.com/phds/project...
Reposted by Pablo Carravilla
‼️3 postdoc positions open‼️
We are building a quantitative imaging team at Warwick!
Join an interdisciplinary endeavor with Mishima, Balasubramanian, and Mayor labs on a @wellcometrust.bsky.social funded project to push probe and microscopy development!
warwick-careers.tal.net/vx/lang-en-G...
We are building a quantitative imaging team at Warwick!
Join an interdisciplinary endeavor with Mishima, Balasubramanian, and Mayor labs on a @wellcometrust.bsky.social funded project to push probe and microscopy development!
warwick-careers.tal.net/vx/lang-en-G...
a logo that says let 's do this in blue and yellow
ALT: a logo that says let 's do this in blue and yellow
media.tenor.com
December 13, 2024 at 11:51 AM
‼️3 postdoc positions open‼️
We are building a quantitative imaging team at Warwick!
Join an interdisciplinary endeavor with Mishima, Balasubramanian, and Mayor labs on a @wellcometrust.bsky.social funded project to push probe and microscopy development!
warwick-careers.tal.net/vx/lang-en-G...
We are building a quantitative imaging team at Warwick!
Join an interdisciplinary endeavor with Mishima, Balasubramanian, and Mayor labs on a @wellcometrust.bsky.social funded project to push probe and microscopy development!
warwick-careers.tal.net/vx/lang-en-G...
Reposted by Pablo Carravilla
Hi Bluesky! 👋
Here is our latest collaborative work on long-term (up to 20 min) single-molecule tracking in living cells using exchangeable HaloTag Ligands 🔬
#ChemBio #microscopy
onlinelibrary.wiley.com/doi/10.1002/...
Here is our latest collaborative work on long-term (up to 20 min) single-molecule tracking in living cells using exchangeable HaloTag Ligands 🔬
#ChemBio #microscopy
onlinelibrary.wiley.com/doi/10.1002/...
Long‐Term Single‐Molecule Tracking in Living Cells using Weak‐Affinity Protein Labeling
A self-labeling protein tag in combination with exchangeable fluorophore ligands bypasses photobleaching and enables long-term single-molecule imaging in living cells. This extends observation times ...
onlinelibrary.wiley.com
November 27, 2024 at 8:24 AM
Hi Bluesky! 👋
Here is our latest collaborative work on long-term (up to 20 min) single-molecule tracking in living cells using exchangeable HaloTag Ligands 🔬
#ChemBio #microscopy
onlinelibrary.wiley.com/doi/10.1002/...
Here is our latest collaborative work on long-term (up to 20 min) single-molecule tracking in living cells using exchangeable HaloTag Ligands 🔬
#ChemBio #microscopy
onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Pablo Carravilla
The dark side of fluorescent protein tagging: the impact of protein tags on biomolecular condensation
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 25, 2024 at 12:19 PM
The dark side of fluorescent protein tagging: the impact of protein tags on biomolecular condensation
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...