Luciano A. Masullo
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lumasullo.bsky.social
Luciano A. Masullo
@lumasullo.bsky.social
810 followers 370 following 75 posts
Physicist | single molecules, bioscience, biotech, nanotech, optics, fluorescence | Project Leader at @jungmannlab.bsky.social Max Planck Institute of Biochemistry @mpibiochem.bsky.social
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Luciano A. Masullo @lumasullo.bsky.social · Jul 30
Ångström-resolution imaging of cell-surface glycans

Now published in Nature Nanotechnology! 🤩
@natnano.nature.com

www.nature.com/articles/s41...
Reposted by Luciano A. Masullo
yongdengzhang.bsky.social
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods
4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.
www.nature.com
November 21, 2025 at 3:00 AM
Reposted by Luciano A. Masullo
events.embl.org
We're excited to announce the poster prize winners of #EESImaging 🔬

A special poster prize committee elected three winners:
Alara Kiris, @lumasullo.bsky.social and @wanluzhang.bsky.social. Congratulations!

Read on to learn about their research: s.embl.org/ees25-09post...
October 30, 2025 at 12:21 PM
Reposted by Luciano A. Masullo
natnano.nature.com
Now online: October 2025 Issue.

- Focus Issue on #biosensing,
- DNA moiré superlattices,
- Sugars at Ångström-resolution,
- Solid-state #nanopores,
- Non-aqueous Li #batteries, -
- Neuromorphic vision,
- Peptide #hydrogels,
- Deep learning for #LNPs and more...

www.nature.com/nnano/volume...
The image on the cover shows two sugars from the same cell-surface glycan separated by 9 Å, visualized with RESI (resolution enhancement by sequential imaging) enabled by metabolic labelling with DNA barcodes. IMAGE: Luciano A. Masullo, Max Planck Institute of Biochemistry, Germany. COVER DESIGN: Vanitha Selvarajan Original paper: Masullo, L.A., et al. Ångström-resolution imaging of cell-surface glycans. Nat. Nanotechnol. 20, 1457–1463 (2025). https://doi.org/10.1038/s41565-025-01966-5 Abstract: Glycobiology is rooted in the study of monosaccharides, ångström-sized molecules that are the building blocks of glycosylation. Glycosylated biomolecules form the glycocalyx, a dense coat encasing every human cell with central relevance—among others—in immunology, oncology and virology. To understand glycosylation function, visualizing its molecular structure is fundamental. However, the ability to visualize the molecular architecture of the glycocalyx has remained challenging. Techniques such as mass spectrometry, electron microscopy and fluorescence microscopy lack the necessary cellular context, specificity and resolution. Here we combine resolution enhancement by sequential imaging with metabolic labelling, enabling the visualization of individual sugars within glycans on the cell surface, thus obtaining images of the glycocalyx with a spatial resolution down to 9 Å in an optical microscope.
October 17, 2025 at 6:45 PM
lumasullo.bsky.social
Our article "Ångström-resolution imaging of cell-surface glycans" made it to the cover of Nature Nanotechnology! 🤩 @natnano.nature.com

www.nature.com/nnano/volume...
October 18, 2025 at 2:32 PM
lumasullo.bsky.social
Thrilled to announce some big news!

Excited to share that I’ve been awarded a Wellcome Trust Early Career Award to establish my research as a Group Leader in the Glycosciences Program at the Institute of Biology and Experimental Medicine (IBYME) in Buenos Aires, Argentina, beginning in early 2026.
October 6, 2025 at 6:24 AM
Reposted by Luciano A. Masullo
jungmannlab.bsky.social
Within this neuronal atlas we can reveal the three synapse classes, excitatory, inhibitory and the recently discovered mixed synapse. Organelle imaging of Peroxisomes (Pmp70) and the Golgi Apparatus (Golga5) reveals rare contact sides and even fused particles. (5/6)
October 2, 2025 at 11:37 AM
Reposted by Luciano A. Masullo
jungmannlab.bsky.social
To show the power of the technique, we acquired a 13-plex 200 x 200 µm2 neuronal atlas in 3D. With this atlas we map the interaction architecture of three neurons, resolving organelles, cytoskeleton, vesicles and synapses at single-protein resolution. (4/6)
October 2, 2025 at 11:37 AM
Reposted by Luciano A. Masullo
jungmannlab.bsky.social
We demonstrate speed-optimized left-handed DNA-PAINT by characterizing the sequence binding kinetics and resolving three main microscopy benchmarking targets, mitochondria, microtubules and nuclear pore complexes with <5 nm localization precision. (3/6)
October 2, 2025 at 11:37 AM
Reposted by Luciano A. Masullo
jungmannlab.bsky.social
The mirrored design of left-handed oligonucleotides allows the extension of the common 6 speed-sequences R1-R6 with their analogs L1-L6, enabling 12 target multiplexing with a standard secondary label-free DNA-PAINT workflow. (2/6)
October 2, 2025 at 11:37 AM
Reposted by Luciano A. Masullo
jungmannlab.bsky.social
Highly efficient 12-color multiplexing with speed-optimized DNA-PAINT. We are excited to share our latest paper in @natcomms.nature.com, using left-handed DNA to extend speed-optimized DNA-PAINT to 12 targets in a simple and straightforward way! 🧬👈🚀https://www.nature.com/articles/s41467-025-64228-x
October 2, 2025 at 11:37 AM
Reposted by Luciano A. Masullo
mpi-mr.bsky.social
📣 📅 Invitation to two talks at our institute next Tuesday, October 7: 🤩 Luciano Masullo @lumasullo.bsky.social from @mpibiochem.bsky.social at 10 am, Adam Cohen @harvard.edu at 3pm, also online:
us06web.zoom.us/webinar/regi...

#maxplanck #mpimr #colloquium #medicalresearch #sciencetalks #science
One talk by Luciano Masullo at 10 am and another talk at 3 pm by Adam Cohen, both next Tuesday, October 7, at the MPI for Medical Research Heidelberg.
October 2, 2025 at 10:33 AM
lumasullo.bsky.social
Happy to share this article with my views on @elislenders.bsky.social and @vicidominilab.bsky.social work and discussing current developments in single-molecule localization combining structured excitation and detection. So many exciting perspectives in the field!

www.nature.com/articles/s41...
Localization of single molecules with structured illumination and structured detection - Light: Science & Applications
Light: Science & Applications - Localization of single molecules with structured illumination and structured detection
www.nature.com
September 30, 2025 at 6:17 PM
lumasullo.bsky.social
Ångström-resolution imaging of cell-surface glycans

Now published in Nature Nanotechnology! 🤩
@natnano.nature.com

www.nature.com/articles/s41...
July 30, 2025 at 2:58 PM
Reposted by Luciano A. Masullo
natnano.nature.com
New paper online:

Ångström-resolution imaging of cell-surface glycans.

The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.

#Glycotime

www.nature.com/articles/s41...
Ångström-resolution imaging of cell-surface glycans - Nature Nanotechnology
By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...
www.nature.com
July 28, 2025 at 9:44 AM
Reposted by Luciano A. Masullo
jungmannlab.bsky.social
Congratulations to everyone involved: @ipachmayr.bsky.social, @lumasullo.bsky.social, @susannereinhardt.bsky.social, Jisoo Kwon, Ondřej Skořepa, Maite Llop, Sylvia Herter, Marina Bacac and Christian Klein. (6/6)
Read the full story here: www.nature.com/articles/s41...
Resolving the structural basis of therapeutic antibody function in cancer immunotherapy with RESI - Nature Communications
The nanoscale organization of the antigen-antibody complexes influences the therapeutic action of monoclonal antibodies. Here, the authors present a multi-target 3D RESI imaging assay for the nanomete...
www.nature.com
July 28, 2025 at 8:36 AM
Reposted by Luciano A. Masullo
ceciliazaza.bsky.social
Can we break past the usual limits of #SuperResolution #Microscopy—achieving deep-imaging, high-res, large FOV imaging without the trade-offs?
🧬🔬 YES: with #DNA_PAINT + Spinning Disk Confocal + Optical Photon Reassignment (#SDC-OPR)!
📄 www.nature.com/articles/s41... @natcomms.nature.com!
🧵👇
Super-resolution imaging in whole cells and tissues via DNA-PAINT on a spinning disk confocal with optical photon reassignment - Nature Communications
Zaza and colleagues demonstrate that DNA-PAINT on a spinning disk confocal microscope with optical photon reassignment enables high-resolution imaging across large fields and imaging depths, resolving...
www.nature.com
May 30, 2025 at 3:10 PM
Reposted by Luciano A. Masullo
nanotag.bsky.social
A new study by @jungmannlab.bsky.social presents #SPINNA, a model-based tool for quantifying protein stoichiometry, oligomerization state, and spatial arrangement from super-resolution data.🔬For precise labeling of #GFP & #mCherry fusion proteins researchers used our #nanobodies 😊
📄 bit.ly/4jTHfes
Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications
Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...
bit.ly
May 13, 2025 at 10:12 AM
lumasullo.bsky.social
Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩

We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨
May 7, 2025 at 2:56 PM
Reposted by Luciano A. Masullo
jungmannlab.bsky.social
Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution

We are excited to present our latest work published in @natcomms.nature.com

www.nature.com/articles/s41...
Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications
Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...
www.nature.com
May 7, 2025 at 2:43 PM
Reposted by Luciano A. Masullo
adamkglaser.bsky.social
To look into: a Python alternative to ZEMAX... github.com/HarrisonKram...
GitHub - HarrisonKramer/optiland: Comprehensive optical design, optimization, and analysis in Python, including GPU-accelerated and differentiable ray tracing via PyTorch.
Comprehensive optical design, optimization, and analysis in Python, including GPU-accelerated and differentiable ray tracing via PyTorch. - HarrisonKramer/optiland
github.com
May 2, 2025 at 10:56 PM
lumasullo.bsky.social
I had a blast visiting the lab of Prof. Peng Xi at Peking University!!

Prof. Xi and his team are doing amazing work on various type of SIM and other imaging modalities!

Thanks so much for the warm welcome, the stimulating discussions, and the campus tour!!
April 30, 2025 at 7:43 AM
lumasullo.bsky.social
Amazing visit to the lab of Prof. Wei Ji at the Institute of Biophysics (CAS) in Beijing today!

They’re doing top-notch research, including awesome interferometric single-molecule localization methods (ROSE)

thanks so much for the invitation, warm welcome and hospitality!! 😍🤩
April 28, 2025 at 11:51 AM
lumasullo.bsky.social
#FOM2025 in Taiwan starting now!! Looking forward to great talks and also to tell about RESI and our latest work!
April 13, 2025 at 6:08 AM
Reposted by Luciano A. Masullo
moniquehonsa.bsky.social
IMAGING LIGAND-RECEPTOR INTERACTIONS AT SINGLE-PROTEIN RESOLUTION WITH DNA-PAINT🔬
Ever wonder how cells "talk"? It starts when ligands bind to receptors on cell surfaces. We have cracked the challenge of imaging small ligands on cell surfaces. #DNAPAINT #celltalk 💬
doi.org/10.1002/smtd...
April 10, 2025 at 11:40 AM
lumasullo.bsky.social
Check-out this powerful application of DNA-PAINT and RESI: imaging ligand-receptor interactions at molecular resolution!

Project led by my extremely talented colleagues Monique Honsa, Larissa Heinze and Isabelle Pachmayr

Very happy to have contributed to this beautiful work! 🤩
moniquehonsa.bsky.social Monique Honsa @moniquehonsa.bsky.social · Apr 10
IMAGING LIGAND-RECEPTOR INTERACTIONS AT SINGLE-PROTEIN RESOLUTION WITH DNA-PAINT🔬
Ever wonder how cells "talk"? It starts when ligands bind to receptors on cell surfaces. We have cracked the challenge of imaging small ligands on cell surfaces. #DNAPAINT #celltalk 💬
doi.org/10.1002/smtd...
April 10, 2025 at 2:05 PM