Luiz Passalacqua
@luizpassalacqua.bsky.social
RNA and DNA geek | Structure-function of nucleic acids | Assistant Professor at the University of Florida | My views. Website: https://sites.google.com/view/passalacqualab/
Reposted by Luiz Passalacqua
Reposted by Luiz Passalacqua
Excited to share my latest research. In this paper in PNAS, I use #cryoEM and #crystallography to solve multiple new structures of a viral RNA motif that controls frameshifting.
#virus #retrovirus #structuralbiology #science #biochemistry
www.pnas.org/doi/10.1073/...
#virus #retrovirus #structuralbiology #science #biochemistry
www.pnas.org/doi/10.1073/...
Structural switching dynamically controls the doubly pseudoknotted Rous sarcoma virus–programmed ribosomal frameshifting element | PNAS
A hallmark of retrovirus replication is the translation of two different polyproteins
from one RNA through programmed –1 frameshifting. This is a m...
www.pnas.org
April 2, 2025 at 4:36 PM
Excited to share my latest research. In this paper in PNAS, I use #cryoEM and #crystallography to solve multiple new structures of a viral RNA motif that controls frameshifting.
#virus #retrovirus #structuralbiology #science #biochemistry
www.pnas.org/doi/10.1073/...
#virus #retrovirus #structuralbiology #science #biochemistry
www.pnas.org/doi/10.1073/...
Reposted by Luiz Passalacqua
Our paper describing our Transcription Elongation Complex Display platform for high-throughput cotranscriptional RNA functional assays is now online at @naturecomms.bsky.social: www.nature.com/articles/s41.... We used TECdisplay to assess the activity of >1M ZTP riboswitch variants!
Systematic analysis of cotranscriptional RNA folding using transcription elongation complex display - Nature Communications
Understanding how RNA folds into functional structures requires methods that can systematically assess how sequence perturbations affect RNA structure and function. Here, the authors develop a broadly...
www.nature.com
March 10, 2025 at 2:04 PM
Our paper describing our Transcription Elongation Complex Display platform for high-throughput cotranscriptional RNA functional assays is now online at @naturecomms.bsky.social: www.nature.com/articles/s41.... We used TECdisplay to assess the activity of >1M ZTP riboswitch variants!
Reposted by Luiz Passalacqua
Our latest preprint, led by Skyler Kelly: we developed a platform for high-throughput cotranscriptional RNA biochemistry called Transcription Elongation Complex display. www.biorxiv.org/content/10.1...
Systematic analysis of cotranscriptional RNA folding using transcription elongation complex display
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
December 26, 2023 at 3:57 PM
Our latest preprint, led by Skyler Kelly: we developed a platform for high-throughput cotranscriptional RNA biochemistry called Transcription Elongation Complex display. www.biorxiv.org/content/10.1...
Reposted by Luiz Passalacqua
The final version of our article describing a modernized procedure for cotranscriptional RNA structure probing is online! www.nature.com/articles/s41...
Observation of coordinated RNA folding events by systematic cotranscriptional RNA structure probing ...
RNA begins to fold as the nascent transcript emerges from a transcribing RNA polymerase. Here, the authors develop a concise method for mapping RNA folding pathways that couples in vitro transcription...
www.nature.com
November 29, 2023 at 2:46 PM
The final version of our article describing a modernized procedure for cotranscriptional RNA structure probing is online! www.nature.com/articles/s41...